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1.
目的 研究鞘内注射细胞外调节蛋白激酶（ERK1/2）抑制剂（SCH772984）对骨癌痛Sprague-Dawley（SD）大鼠疼痛行为学及脊髓背角Fos蛋白表达的影响,探讨ERK-P90RSK-Fos信号通路在骨癌痛中的作用。方法 取鞘内置管后5天的雌性SD大鼠40只,随机分成5组（n=8）,包括Sham假手术组和BNP模型组（分别为对照组,SCH772984给药组SCH 0.1组,SCH 1组,SCH 10组）。在造模后第9天,Sham组不给药,BNP模型组鞘内分别给5% DMSO 10μl、SCH772984抑制剂 0.1、1.0、10μg（SCH772984抑制剂溶于10μl 5%的DMSO中）。测定造模前1天、造模后3、6、9、12、15、18天以及给药后1、3、6、9、12、18、24h的机械缩足阈值（MWT）、热缩足潜伏期（PWL）以及2min自发缩足次数。取鞘内置管后5天的SD大鼠40只,随机分为5组（n=8）,其中B1、B2、B3组在造模后第9天,鞘内注射SCH772984抑制剂10μg后分别于1、9、24h取材,M组为模型对照组,鞘内注射5% DMSO后9h取材,S组为空白对照组。通过免疫印迹（Western blot）法及免疫荧光测定脊髓背角p-ERK、p-p90RSK以及Fos蛋白表达情况。结果 鞘内注射ERK1/2抑制剂（SCH772984）对骨癌痛大鼠有镇痛作用,并且该效应随着剂量的增加而增大;鞘内注射ERK1/2抑制剂（SCH772984）10μg可明显减少脊髓背角Fos蛋白的表达。结论 ERK-p90RSK-Fos通路可能影响骨癌痛。 相似文献
2.
目的 初步探讨P16INK4A联合HPV L1壳蛋白检测在宫颈鳞状上皮内病变诊断中的应用价值。方法 选择在温州市人民医院就诊患者,收集69例宫颈鳞状上皮内病变标本,行P16INK4A、HPV L1壳蛋白检测,分析P16INK4A、HPV L1壳蛋白及P16INK4A联合HPV L1壳蛋白在各级宫颈病变中的表达差异。结果 P16INK4A在LSIL组、HSIL组、SCC组中的阳性率分别为45.5%、85.2%、100.0%。同LSIL组比较,P16INK4A在HSIL组及SCC组阳性率明显升高（P<0.01）。HPV L1壳蛋白在LSIL组、HSIL组、SCC组中的阳性率分别为63.6%、18.5%、0%,HPV L1壳蛋白在LSIL组中阳性率明显低于HSIL组及SCC组（P<0.01）。随着宫颈病变加重,P16+/L1-阳性率有升高趋势（P<0.01）,P16-/L1+阳性率有下降趋势（P<0.01）。LSIL组中P16+/L1-阳性率明显低于HSIL组和SCC组（P<0.01）,SCC组P16+/L1-阳性率较HSIL组有明显提高（P<0.05）。LSIL组中P16-/L1+阳性率明显低于HSIL组和SCC组（P<0.01）。P16+/L1-较P16INK4、HPV L1壳蛋白单一检测筛查HSIL+特异性明显升高（P<0.01）。结论 P16INK4A联合HPV L1壳蛋白有望成为诊断宫颈鳞状上皮内病变的有效指标。 相似文献
3.
目的 探讨平均血小板体积等参数在急性胰腺炎病程中变化的临床意义及其对治疗效果评估的价值。方法 回顾性分析53例轻症急性胰腺炎（MAP）患者、25例重症急性胰腺炎（SAP）患者和40例健康者（对照组）的临床资料,检测平均血小板体积（MPV）、血小板计数（PLT）、血小板分布宽度（PDW）、白细胞计数（WBC）、C反应蛋白（CRP）及血清钙离子浓度（Ca2+）等指标。比较并分析上述指标在急性胰腺炎病程中及治疗前后的变化。结果 与对照组比较,急性胰腺炎患者入院时MPV显著升高,差异具有统计学意义（P<0.05）;与MAP组比较,SAP组入院时MPV明显升高,差异具有统计学意义（P<0.05）;SAP组中,MPV在入院后第3天较入院时进一步升高,至入院第5天、第7天逐渐下降;SAP组中,MPV在病程中各时间点与MAP组比较均显著升高,差异有统计学意义（P<0.05）;与治疗前比较,急性胰腺炎患者治疗后MPV显著降低,差异有统计学意义（P<0.05）。结论 急性胰腺炎患者平均血小板体积与病情严重程度及治疗效果相关。 相似文献
4.
目的 探究持续封闭负压引流技术（VSD）对瘢痕切除创面成纤维细胞生长环境的影响。方法 回顾性分析2014年1月~2015年11月于笔者医院行植皮手术的四肢瘢痕切除术后患者60例,根据植皮手术前是否接受VSD治疗分为负压组（n=30）和包扎组（n=30）,负压组均于一期植皮前接受VSD治疗,包扎组未接受VSD治疗。回顾性分析对比两组患者并发症发生率、首次植皮存活率、创面感染率、住院时间之间的差异。并用TUNEL染色、CD34染色法对比瘢痕标本中成纤维细胞凋亡指数及血管生成情况。结果 负压组菌培养阳性率明显低于包扎组（P<0.05）,植皮存活率明显高于包扎组（P<0.05）。两组并发症发生率、住院时间、疼痛评分、随访VSS评分间比较,负压组均明显较低（P<0.05）。负压组成纤维细胞凋亡指数明显较低,微血管计数明显较高（P<0.05）。结论 负压封闭引流可以减轻瘢痕切除创面感染率,有效减轻炎性水肿,且可以通过改变局部血液循环微环境以及机械应力作用促进成纤维细胞增殖分裂,提高创面恢复效果。 相似文献
5.
目的 比较三叉神经感觉根梳理术和半月节射频术治疗原发性三叉神经痛的临床效果,揭示其临床意义。方法 对37例没有明显血管压迫的且行颅内段三叉神经感觉根梳理术治疗的原发性三叉神经痛患者（梳理术组）和37例行三叉神经半月节射频术治疗的原发性三叉神经痛患者（射频术组）进行3年随访,记录和比较两组患者术后疼痛治疗有效率、复发率和颜面部麻木发生率。结果 梳理术组和射频术组术后3年有效率分别为78.4%（29/37）和73.0%（27/37）,术后3年复发率分别是21.6%（8/37）和27.0%（10/37）,术后颜面部麻木发生率分别为91.9%（34/37）和100%（37/37）,组间比较差异均无统计学意义（P均>0.05）。结论 三叉神经感觉根梳理术和半月节射频术是治疗原发性三叉神经痛较为理想的手段,考虑到开颅手术的风险,三叉神经半月节射频术可能更合适于治疗没有明显血管压迫的原发性三叉神经痛老年患者。 相似文献
6.
目的 评价甲强龙联合右美托咪定对体外循环（CPB）心脏瓣膜置换术患者脑认知功能的保护作用。方法 选择内蒙古医科大学附属医院CPB下行心脏瓣膜置换术患者60例,完全随机化分为4组（n=15）,分别为对照组（A组）、甲强龙组（M组）、右美托咪定组（D组）和甲强龙联合右美托咪定组（B组）,在麻醉诱导后气管插管,机械通气。M组在体外循环预冲液加入甲强龙20mg/kg,D组麻醉在诱导后静脉泵注右美托咪定1μg/kg负荷量,之后以0.05μg/（kg·h）速率静脉泵注至术毕,B组既麻醉诱导后静脉注射右美托咪定1μg/kg负荷量,然后以0.05μg/（kg·h）速率静脉输注至术毕又在体外循环预冲液加入甲强龙20mg/kg,A组给于等容量生理盐水。在麻醉后手术前（T1）、CPB即刻（T2）、CPB30min（T3）、CPB结束10min（T4）、术毕（T5）、CPB结束后6h（T6）时采集颈内静脉球部血样,使用酶联免疫吸附实验（ELISA）法测定血清IL-6、IL-10、S100β蛋白和神经元特异性烯醇化酶（NSE）的浓度。记录术前1天术后2天、3天、7天采用简化智能评分量表（MMSE）评估患者认知功能。结果 M组、D组和B组在T1~6各个时间点IL-6、S100β蛋白和NSE均比对照组明显降低,IL-10均比对照组升高,差异有统计学差异（P<0.05）。B组在T1~6各个时间点IL-6、S100β蛋白和NSE均比其他组更低,IL-10均比其他组更高,差异有统计学意义（P<0.05）。M组、D组和B组术后发生认知功能障碍的发生率均高于对照组,B组发生认知功能障碍的发生率最低,差异有统计学意义（P<0.05）。结论 甲强龙和右美托嘧啶均可以有效降低体外循环中IL-6、S100β蛋白和NSE的浓度,降低脑损伤,减少术后认知功能障碍的发生率,联合应用甲强龙和右美托嘧啶对体外循环后脑损伤的保护效果更佳。 相似文献
7.
XiMing Chen Ling Zhang HaiLi Sun ShuYan Li SiJing Chang QingFeng Zhang BingLin Zhang Tuo Chen GuangXiu Liu Paul Dyson 《寒旱区科学》2017,9(5):503-510
目的 探讨脓毒症患者中横纹肌溶解综合征（RM）与病情严重程度及预后的关系。方法 采用前瞻性研究方法,入选2014年10月~2015年9月重庆医科大学附属第一医院中心ICU连续收治的脓毒症患者151例作为研究对象。检测入ICU当天的血清肌酸激酶（CK）,以CK≥1000U/L作为RM的诊断标准,比较发生RM的患者与未发生RM患者的感染标志物、危重症评分、器官功能障碍和28天病死率等。结果 151例脓毒症患者中37例（24.5%）发生RM。RM组的PCT水平、APACHEⅡ评分和SOFA评分较非RM组高（P<0.05）。RM组ICU期间肝脏功能障碍、循环障碍、肾脏功能障碍的发生率、人均器官障碍数、血肌酐峰值水平、接受连续肾脏替代治疗（CRRT）患者的比例和28天病死率较非RM组高（P<0.05）,而呼吸功能障碍、凝血功能障碍和神经系统障碍的发生率比较,差异无统计学意义（P>0.05）。CK对预测脓毒症患者28天死亡风险具有统计学意义,ROC曲线下面积（AUC）为0.694（P<0.05）,低于APACHEⅡ评分（0.841）和SOFA评分（0.805）（P<0.05）。结论 脓毒症患者中RM的发生率高,发生RM的患者的病情更危重,其ICU期间肝脏功能障碍、循环障碍、肾脏功能障碍的发生率和28天病死率均显著增加,CK可以作为预测脓毒症患者28天病死率的参考指标。 相似文献
8.
目的 在前期蛋白质组学研究中,明确了一系列候选血浆差异蛋白指标,包括RelB、PSMD10 等含量上调可能是宫颈癌的候选血浆蛋白质指标。本研究对上述指标进行定量验证,探讨宫颈癌发生及进展与血浆蛋白质表达调控的关系及临床意义。方法 收集维吾尔族女性宫颈病变患者外周血标本共70例,其中宫颈炎（cervicitis,CV）20例,宫颈内上皮瘤变（cervical intraepithelial neoplasisa,CIN）Ⅱ~Ⅲ 20例及宫颈鳞癌（cervical squamous cell carcinoma,CSCC）30例,采用酶联免疫法对血浆RelB、PSMD10 表达水平进行定量鉴定。结果 宫颈病变进展伴有血浆RelB、PSMD10 蛋白质含量梯度性上调,其差异有统计学意义（P<0.05）。通过不同血浆蛋白质含量变化的相关性分析,发现血浆PSMD10与RelB呈低度正相关（r<0.4,P<0.01）。对不同蛋白质指标的检测对宫颈癌及癌前病变的预测能力分析,发现血浆RelB蛋白质检测的敏感度、特异性和准确度较高（依次为90.0%、50.0%和75.7%）,对宫颈癌诊断可能有较高的预测价值。结论 宫颈癌发生与血浆蛋白质表达调控存在密切关系,血浆RelB、PSMD10 含量上调可能是宫颈癌发生的早期预警的血浆检测指标,对其检测对宫颈癌诊断具有较高的预测价值。 相似文献
9.
目的 观察内科胸腔镜在不明原因胸腔积液中的诊断价值及操作方法。方法 自2009年4月~2015年6月,共149例患者于笔者医院呼吸科行胸腔镜检查,根据病因分为肺炎旁积液、脓胸、结核性胸膜炎、恶性胸腔积液4组,观察各组的胸腔镜下胸膜表现,胸腔积液外观、常规、生化、肿瘤标志物、细胞学及结核涂片检查的特点;观察胸腔镜操作前人工气胸以及操作后置管引流的情况;并观察并发症的发生情况。结果 内科胸腔镜的确诊率为87.2%。不同病因所导致的胸腔积液,其胸腔镜下表现是不同的。粘连最重的是脓胸和结核性胸膜炎。结核性胸膜炎和恶性胸腔积液的胸膜结节表现不同。胸腔积液的各项检查有助于进一步确诊。术前通过胸腔引流管行人工气胸,注气量平均为588±276ml。术后留置细管比例为69.4%（109/157）,带针粗引流管比例为21.0%（33/157）。最常见并发症为皮下气肿（38/157,24.2%）,少量患者出现胸痛（24/157,15.3%）、低热（2/157,1.3%）。结论 胸腔镜检查能够明显提高不明原因胸腔积液的诊断率;在局麻镇静下即可完成操作,术前建议给予人工气胸,术后建议留置细管,并且操作过程安全,并发症少,值得临床推广。 相似文献
10.
目的 探讨铁过载对原代培养脐静脉内皮细胞（HUVECS）超氧化物歧化酶（SOD）、活性氧表达的影响及川芎嗪的干预效应。方法 使用酶联免疫法（ELISA）检测体外培养铁过载脐静脉内皮细胞表达的超氧化物歧化酶和活性氧的含量。结果 与空白组比较,模型组超氧化物歧化酶的含量显著降低（P<0.01）,给药组超氧化物歧化酶与模型组比较,差异有统计学意义（P<0.05）;模型组活性氧含量显著高于空白组（P<0.01）,与模型组相比,给药组活性氧含量有明显降低（P<0.05）。结论 川芎嗪能抑制脐静脉内皮细胞铁过载导致的氧化应激,有通过抗氧化应激发挥抗动脉粥样硬化的作用。 相似文献
11.
Evidence of late Holocene climate variability in the western Nanling Mountains,South China 总被引：1，自引：0，他引：1
Wei Zhong Jibin Xue Jun Ouyang Jiayuan Cao Zhanghong Peng 《Journal of Paleolimnology》2014,52(1-2):1-10
A 95-cm-thick peat sequence obtained from Daping Swamp in the western Nanling Mountains provides evidence for climate variability in the past ~3,000 year. Multi-proxy records (including organic carbon isotopes, humification degree, organic matter content, and dry bulk density) revealed three intensified Asian summer monsoon (ASM) intervals (i.e.~2900–2700, 2500–1700 and 1000–600 cal. yr BP) and three weakened ASM intervals (i.e.~2800–2500, 1700–1000 and 600–200 cal. yr BP). Our δ13C record shows a possible correlation with the sunspot number and residual atmospheric 14C records on multi-centennial scale, especially for the period between 2960 and 2200 cal. yr BP. A spectral analysis of δ13C record reveals three significant cycles (i.e., 396, 110 and 102 yr) and all these cycles could be related to solar activity, suggesting that solar output may have influenced the late Holocene climate variability in the study region. 相似文献
12.
《The Journal of geography》2012,111(6):266-267
Abstract Imtnigrazione e Multicultura nell'Italia di Oggi: il Territorio, i Problemi, la Didattica, Carlo Brusa, editor, Milan, Italy: Franco Angelli. 1997. ISBN 88-464- 0246-4. (paper). 相似文献
13.
《The Professional geographer》2013,65(4):452-458
The foundations of modern Romanian geography were laid by one man, Simion Mehedin?i, at the beginning of the twentieth century. His systematic view of a unified geography developed from the ideas formed during his education in France and Germany, particularly under the influence of Ratzel. His ideas, propagated by his followers, are still regarded as valid by the present generation of Romanian geographers. 相似文献
14.
《The Journal of geography》2012,111(3):100-103
Abstract Computer technology has a rich history in geography education. The Internet represents the latest in technological advancements that continue to have important effects on geography curriculum and instruction. Many geographers are involved with Internet-based instruction, which some educators believe has potential to facilitate changes in how we teach and even what we teach. This article describes how the Internet is being used by some geographers for instructional purposes and discusses opportunities for improving teaching and learning with the Internet. 相似文献
15.
16.
《The Journal of geography》2012,111(3):33-45
Abstract Growth of industrial/office parks in metropolitan areas is the result of the truck-auto freeway complex, changes in technology, increased i market orientation of activity, and the desire for facilities with favorable public images. This article reviews the historical development of industrial and office parks, and speculates about their behavioral characteristics at the national and metropolitan scale, including the process of decentralization. The locational and functional characteristics of these parks in Atlanta, Georgia are treated as being typical of those in a regional capital. Readers no doubt will be familiar with similar developments in their own communities and can use this paper as a resource to guide their investigations. 相似文献
17.
According to the glacial landforms and deposits with the optically stimulated luminescence (OSL) dating results, two glacial
stages of the last glacial cycle (LGC) and Late Glacial were identified. The Late Glacial stage (Meteorological Station glacier
advance) took place about 11 ka (11.3±1.2 ka), and the last glacial maximum (LGM), named Black Wind Mouth glacier advance,
occurred at 20 ka (20.0±2.1 ka). Based on the Ohmura’s formula in which there is a relationship between summer (JJA) atmospheric
temperature (T) and the annual precipitation (P) at ELA, the present theoretical equilibrium line altitude (ELAt) in Changbai Mountains was 3380±100 m. Six methods of accumulation-area ratio (AAR), maximum elevation of lateral moraines
(MELM), toe-to headwall altitude ratios (THAR), the terminal to summit altitudinal (TSAM), the altitude of cirque floor (CF),
and the terminal to average elevation of the catchment area (Hofer) were used for calculation of the former ELAs in different
stages. These methods provided the ELA for a range of 2250–2383 m with an average value of 2320±20 m during the LGM, which
is 200 m higher than the value of previous investigation. The snowlines during the Late Glacial are 2490 m on northern slope,
and 2440 m on western slope. The results show that the snowline on northern slope is 50 m higher than that on western slope
during the Late Glacial, and the average snowline is 2465m. The ΔELA values were more than 1000 m during the LGM, and about
920 m lower than now during the Late Glacial stage respectively. Compared with Taiwanese and Japanese mountains in East Asia
during the LGM, the effect of the uplift on ELA in Changbai Mountains during the glaciations (i.e. 20 m uplift in the LGM
and 11 m in the Late Glacial) is not obvious.
Foundation: National Natural Science Foundation of China, No.40571016
Author: Zhang Wei (1969–), Ph.D and Professor, specialized in Quaternary environment and climate geomorphology. 相似文献
18.
cand. scient. NIELS NIELSEN 《Geografisk tidskrift / udgivet af Bestyrelsen for Det Kongelige danske geografiske selskab》2013,113(1):67-73
Nielsen, Niels: Periglaciale former på kyster opbygget af løse sedimenter—nogle iagttagelser fra Disko, Vestgrønland. Geografisk Tidsskrift 82: 67–73, København, November 12, 1982. The dynamics of ice-foot and sea ice and the resulting morphological effect on arctic coasts are described, focussing upon the ice as sediment-transporting factor and the thermokarst-like development of forms on the foreshore during the melt period. SAMMENDRAG Om vinteren islægges havet omkring arktiske kyster, hvilket betyder en midlertidig afbrydelse af bølgernes påvirkning af kysterne. Men på trods heraf er den littorale zone ikke morfodynamisk inaktiv i denne periode. Observationer foretaget på Disko, Gr0nland, viser, at såvel isfoden som havisen umiddelbart foran isfoden kan have en betydelig effekt på kyster udformet i klassiske sedimenter. Tidevandsforskellen omkring Disko er 2,9 m ved springtid, ogden karakteristiske isfodsdannelse er af typen »tidal platform ice-foot«. lsfodsdannelse på sedimentkyster anses ofte som konserverende for kysten. lagttagelser har dog vist, at bølgeopkastede isblokke og isflager, lastet med sediment, forårsager en thermokarst-lignende formudvikling på stranden. I samme forbindelse er der observeret struktur- og teksturfænomener i såvel sand- som rullestenkysters sedimenter, som kan henf0res til isens påvirkning. 相似文献
19.
《The Journal of geography》2012,111(5):179-190
Abstract Current regionalizations of Africa have limitations in that they are attribute-based and regions are delineated according to national boundaries. Taking the world city network approach as starting point, it is possible to use relational data (i.e., information about the relationships between cities) rather than attribute data, and moreover, it becomes possible to ignore state boundaries by delineating the regions based on the location of the interaction structure between cities. This research uses airline data. A network analysis is performed on the number of passengers who fly between cities in Africa. A subregional map is created based on the results. 相似文献
20.
柴达木盆地东缘祁连圆柏轮宽序列标准化 总被引：3，自引：0，他引：3
Standardization is one of the important procedures in dendroclimatology. We used abundant Qilian juniper （Sabina przewalskii Kom.） tree-ring samples from the eastern margin of the Qaidam Basin to develop a new standardization method, i.e. total growth curve （TGC）. The samples that contained the complete pith and reached to the growing culmination around the 40th-60th year were used to fit TGC, and the generalized negative exponential function was used to fit the curve. Usually, most cores cannot reach the arboreal pith for some reasons and it is difficult to determine the arboreal cambial age. The empirical model of initial radial growth （IRG） was employed to estimate the number of rings missing from the pith by the same data and IRG model explained 90.9% of the variance. When developing the chronology, the cambial ages of cores that contained the complete pith were regarded as beginning from the first year and others were determined by the numbers of missing and included rings in the core. Standardization was accomplished by dividing each tree-ring series by corresponding TGC. The chronologies developed by TGC can retain more low-frequency variational information and TGC helps to develop more reliable tree-ring width chronology. 相似文献