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1.
把 4种微量金属离子以不同的浓度添加到绿色巴夫藻的培养液中 ,结果表明 :Hg2 +和Pb2 +浓度分别在 5μg/L和 2 0 0 μg/L时 ,对绿色巴夫藻的生长有较好的促进作用 ,同时也使叶绿素a的含量提高 ;Hg2 +≥ 2 0 μg/L时 ,绿色巴夫藻的生长受到抑制 ,叶绿素a的含量降低。Pb2 +所有的试验浓度都使绿色巴夫藻生长加快 ,叶绿素a的含量提高 ;Co2 +在 1μg/L时对绿色巴夫藻的生长有最好的促进效果 ,但当Co2 +≥ 5μg/L时 ,开始对生长出现抑制作用 ,而Co2 +所有的试验浓度都使叶绿素a的含量降低 ;Mn2 +浓度在 10 0 μg/L时 ,对促进绿色巴夫藻生长的效果最佳 ,4 0 0 μg/L的浓度开始对生长出现抑制作用 ,但所有Mn2 +的试验浓度对叶绿素a含量的变化影响不显著。 4种微量金属元素都使绿色巴夫藻略微变小。  相似文献   

2.
为研究生物酶采油解堵剂中产蛋白酶菌株的初、复筛选及培养条件优化,从大庆原油样品中筛选菌种,通过水解酪素的透明圈实验及福林酚测蛋白酶酶活的方法进行菌株的初、复筛选;以蛋白酶酶活为优化指标,采用单因素实验对筛选的产蛋白酶菌株的培养基及培养条件进行优化,优化最适培养基:可溶性淀粉为15g/L,蛋白胨为20g/L,酵母膏为20g/L,NaCl为1.0g/L,CaCl2为0.02g/L,Na2HPO4为0.2g/L,NaH2PO4为0.1g/L;在初始pH为6.0、接种量为5%(体积分数)、温度为31℃、摇床转速为160r/min的条件下,培养72h后,菌株的蛋白酶酶活为551.0U/mL,为复筛选菌株的蛋白酶酶活的22.92倍,即为菌株生长繁殖及代谢的最佳条件,能够获得更高的蛋白酶酶活,有利于后续实验的进行.结果表明:菌株产蛋白酶对原油作用效果为发酵液表面张力从作用前的56.2mN/m降低到作用后的30.5mN/m,表面张力显著降低,还有降解降黏原油等效果,具有一定的研究价值.  相似文献   

3.
研究了3种无机盐对隐甲藻(Crypthecodinium cohnii ATCC30556)生长及DHA产量的影响.结果表明隐甲藻可在NaCl为唯一无机盐的培养基中生长;培养基中NaCl质量浓度为6 g/L,Mg2+浓度为13.23 mmol/L,PO43-浓度为7.19 mmol/L可满足藻细胞生长和DHA积累的需求,此时隐甲藻有着最大的生物量和DHA产量,分别为3.31 g/L和499.93 mg/L.  相似文献   

4.
将150条斑马鱼随机等分为5组:2,3,7,8-四氯代二苯并二噁英(TCDD)4个染毒组(染毒剂量分别为0.1μg/L、0.2μg/L、0.4μg/L和0.8μg/L)和空白对照组,水质接触染毒5d后,采用分光光度法测定其肝组织中丙二醛(MDA)含量和超氧化物歧化酶(SOD)、谷胱甘肽-S-转移酶(GST)的活力,研究TCDD对斑马鱼的脂质过氧化作用。结果发现,TCDD各剂量染毒组MDA含量均有所增加,其中0.2μg/L组差异显著(P<0.05),0.4μg/L和0.8μg/L剂量组差异极显著(P<0.01);各染毒组SOD活性均有所下降,其中0.2μg/L、0.4μg/L和0.8μg/L剂量组差异极显著(P<0.01);各染毒组GST活力均降低且差异极显著(P<0.01)。一定剂量的TCDD能引起MDA含量增加,使SOD和GST活力降低,对斑马鱼具有脂质过氧化作用。  相似文献   

5.
Trare amounts of benzene hydrocarbons obtained in Jiaozhou Bay (Qindao) were enriched bysorption on a GDX-102 column and eluted by carbon disulfide. The eluted was concenttaled and then de-temened by capillary column gas cbornatognphy.The contents of virious kinds of benzene hydrocarbons in Jiaozhou Bay coastal water were benzene(22.3-141.6)× 10~(-9)g/L, toluate (15.2-94.0) × 10~(-9) g/L, ethyl benzene(11.8-85.1)×10~(-9) g/L, p -xylene(15.2-78.5) ×10(-9) g/L, m-xylene (10.9-79.4) ×10(-9) g/L, o -xylene (12.4-80.1) x ×10(-9)g/L; iso-propyl(8.4- 73.1) x ×10(-9)g/L, n -propyl (6.9-76.4) ×10(-9) g/L, 1, 3, 5-trimethylbenzene (10.9- 35.9)×10(-9) g/L, 1,2, 4-trimethybenzene (10.0- 38.0)×10(-9) g/L, n - butydriare (8. 1 - 34.6) ×10(-9)g/L. The recovery of benzenehydrocarbons was (85.1 -95.6)%.  相似文献   

6.
从湛江近海海水和海泥分离的229株细菌中,选出对芒果炭疽菌(Colletotrichum gloeosporioides)具有较强拮抗作用的海洋细菌BSW03,抑菌圈直径为14.0 mm。根据菌体形态、生理生化特性和16s rDNA序列分析,BSW03鉴定为枯草芽孢杆菌(Bacillus subtilis)。从9种培养基中筛选出BDPB作为BSW03的发酵培养基,通过单因子碳源、氮源试验和L9(34)正交试验对发酵培养基成分进行优化。结果表明:发酵培养基最佳配方为乳糖20 g/L,牛肉膏15 g/L,K2HPO3.3H2O 0.75 g/L,MgSO4 0.75 g/L,pH自然。转速170 r/min、温度28℃的恒温摇床培养3 d后的无菌发酵液抑菌效果最佳,抑菌圈直径为27.3 mm。  相似文献   

7.
植物生长调节剂对绿色巴夫藻生长的影响   总被引:7,自引:0,他引:7  
研究了二种植物生长调节剂对绿色巴夫藻 ( Pavlova viridis)的生长、叶绿素含量和干重的影响。结果表明 ,在试验浓度范围内 ,2 ,4— D对绿色巴夫藻生长的影响在培养早期比后期明显 ,最适浓度为 0 .1 m g/L ;6-BA质量浓度≥ 1 mg/L时对绿色巴夫藻有致死作用 ,质量浓度为 0 .1~ 0 .8mg/L时 ,对绿色巴夫藻的生长有促进作用 ,促进生长的最适质量浓度为 0 .5mg/L。在一定浓度范围内 ,二种植物生长调节剂都可促进绿色巴夫藻的生长 ,提高叶绿素含量 ,增加试验藻的干重。  相似文献   

8.
文章探讨了含铜土壤中铜的赋存状态 ,并对土壤中铜的物相分析方法进行了研究。结果表明 :用乙二胺浓度为 0 .0 5mol/L(pH =10 )的溶液浸取自由氧化铜 ,用含有Na2 SO3 和NH4 HF2 的H2 SO4 (0 .9mol/L)溶液浸取结合氧化铜 ,用含有硫脲 (2 0 0g/L)的HCl(1.0mol/L)溶液浸取次生硫化铜能获得满意的结果。在最佳浸取条件下对含铜土壤中的铜进行物相分析和基准矿物回收试验 ,结果满意。  相似文献   

9.
把4种微量金属离子以不同的浓度添加到绿色巴夫藻的培养液中,结果表明:Hg^2 和Pb^2 浓度分别在5μg/L和200μg/L时,对绿色巴夫藻的生长有较好的促进作用,同时也使叶绿素a的含量提高;Hg^2≥20μg/L时,绿色巴夫藻的生长受到抑制,叶绿素a的含量降低。Pb^2 所有的试验浓度都使绿色巴夫藻生长加快,叶绿素a的含量提高;C0^2 在1μg/L时对绿色巴夫藻的生长有最好的促进效果,但当C0^2 ≥5μg/L时,开始对生长出现抑制作用,而C0^2 所有的试验浓度都使叶绿素a的含量降低;Mn^2 浓度在100μg/L时,对促进绿色巴夫藻生长的效果最佳,400μg/L的浓度开始对生长出现抑制作用,但所有Mn^2 的试验浓度对叶绿素a含量的变化影响不显著。4种微量金属元素都使绿色巴夫藻略微变小。  相似文献   

10.
研究了3种无机盐对隐甲藻(Crypthecodinium cohnii ATCC30556)生长及DHA产量的影响。结果表明:隐甲藻可在NaCl为唯一无机盐的培养基中生长;培养基中NaCl质量浓度为6g/L,Mg^2+浓度为13.23mmol/L,PO4^3-浓度为7.19mmol/L可满足藻细胞生长和DHA积累的需求,此时隐甲藻有着最大的生物量和DHA产量,分别为3.31g/L和499.93mg/L。  相似文献   

11.
The electrical heating experiments on oil shale sample from Huadian of Jilin were carried out by the pyrolysis method at three different heating rate 2℃/ min,5℃/ min and 10℃/ min in the temperature range of 30℃-750℃ . Heating rate 2℃/ min is considered low,while intermediate one covers the range 5℃/ min and high heating rate is 10℃/ min. The controlling parameters studied were the final pyrolysis temperature and the influence of the heating rate as well as type. The heating rate has an important effect on the pyrolysis of oil shale and the amount of residual carbon obtained therefore. It is found that increasing the heating rate and pyrolysis temperature also increases the production of oil and the total weight loss. Higher heating rates resulted in higher rates of accumulation. The rate of oil and water collection passed through the maximum of different heating rates at different pyrolysis temperatures. Heating rate affected density,oil conversion and oil yield.  相似文献   

12.
1INTRODUCTION With the acceleration of the urbanization and industrial- ization of China, it is inevitable that cultivated land con- vertsto built-up land for industrial, commercial and resi- dential uses. During 1986- 2002 around 168.4×103ha cultivated land had converted to non-agriculture use an- nually (QU et al., 2005). Though the urbanization rate had increased from 17% in the late 1970s to 41% in 2004, China has a long way to go compared with the de- veloped countries whose urbani…  相似文献   

13.
Zoige Plateau wetlands are located in the northeastern corner of the Qinghai-Tibet Plateau.The landscape pattern evolution processes in the Zoige Plateau and their driving factors were identified by analyzing the dynamic changes in landscape modification and conversion and their dynamic rates of alpine wetlands over the past four decades.The results showed that the landscape conversion between wetlands and non-wetlands mainly occurred during the period from 1966 to 1986.The marsh wetland area converted from lake and river wetlands was larger because of swamping compared to other wetland landscapes.Meanwhile,the larger area of marsh wetlands was also converted to lake wetlands more than other types of wetlands.The modification processes mainly occurred among natural wetland landscapes in the first three periods.Obvious conversions were observed between wetland and nonwetland landscapes(i.e.,forestland,grassland,and other landscapes) in the Zoige Plateau.These natural wetland landscapes such as river,lake and marsh wetlands showed a net loss over the past four decades,whereas artificial wetland landscapes(i.e.,paddy field and reservoir and pond wetlands) showed a net decrease.The annual dynamic rate of the whole wetland landscape was 0.72%,in which the annual dynamic rate of river wetlands was the highest,followed by lake wetlands,while marsh wetlands had the lowest dynamic rate.The integrated landscape dynamic rate showed a decreasing trend in the first three periods.The changes in wetland landscape patterns were comprehensively controlled by natural factors and human activities,especially human activities play an important role in changing wetland landscape patterns.  相似文献   

14.
15.
以精制鱼油和豆油作为脂肪源,配制质量分数0.48%、0.97%、1.50%和1.97%等4种不同水平n-3高度不饱和脂肪酸(n-3 HUFA)的饲料,并饲喂斜带石斑鱼幼鱼50 d,研究不同水平n-3 HUFA对斜带石斑鱼(Epinephlus coioides)幼鱼生长及肌肉脂肪酸组成的影响.结果表明:饲料中n-3 HUFA水平对实验鱼增重率、饲料系数、蛋白质效率都有显著影响(p<0.05).以增重率、饲料系数、蛋白质效率为参考指标,通过回归分析,斜带石斑鱼幼鱼对饲料中n-3 HUFA的适宜需要量(质量分数)为1.27%~1.42%;各实验组的鱼体肥满度不受饲料中n-3 HUFA水平的影响,保持在1.4左右;内脏比与肝体比随饲料中n-3 HUFA水平升高而呈现降低趋势;各实验组鱼体肌肉常规营养成分不受饲料中n-3 HUFA水平的影响,肌肉中的n-3 HUFA随饲料n-3 HUFA水平增加而增加.  相似文献   

16.
引入温度递减率,和地表温度一起作为独立参数对加权平均温度进行拟合,并根据所建立的温度递减率模型,分析其对加权平均温度的影响。研究表明,高精度的温度递减率有利于改善加权平均温度模型拟合值的分布,使其不再与地表温度呈现简单的线性关系,即使不同探空站的地表温度相同,它们计算的加权平均温度也不相同,从而提高GPS气象学中PWV的转换精度。  相似文献   

17.
水汽输送及降水模式对流域水文模型及水资源研究具有重要意义。汉江流域水资源对南水北调以及下游生态环境有重要影响,研究汉江流域水汽输送和降水的时空分布特征是水文模拟和水资源环境管理的基础。本文利用NCEP/NCAR再分析资料和汉江流域19个气象站点的降水资料,以及GIS数据库和分析工具,分析了汉江流域的水汽通量、可降水量、实测降水量和降水转换率的计算方法;以汉江流域1998年7月的气候再分析资料和降水资料为例,计算并分析了汉江流域1998年7月可降水量与实测降水量的空间分布特征,以及降水转化率的分布特征;探讨了水汽输送、可降水量、实测降水量,以及降水转化率的相关性和空间分布特征。通过分析汉江流域1981-2010年的可降水量和实际降水量的分布表明,汉江流域可降水量与降水量变化趋势基本相同,降水转换率近年有所增加。  相似文献   

18.
Internal tides generated upon two-dimensional Gaussian topographies of different sizes and steepness are investigated theoretically in a numerical methodology. Compared with previous theoretical works, this model is not restricted by weak topography, but provides an opportunity to examine the influence of topography. Ten typical cases are studied using different values of height and/or width of topography. By analyzing the baroclinic velocity fields, as well as their first eight baroclinic modes, it is found that the magnitude of baroclinic velocity increases and the vertical structure becomes increasingly complex as height increases or width decreases. However, when both height and width vary, while parameter s (the ratio of the topographic slope to the characteristic slope of the internal wave ray) remains invariant, the final pattern is influenced primarily by width. The conversion rate is studied and the results indicate that width determines where the conversion rate reaches a peak, and where it is positive or negative, whereas height affects only the magnitude. High and narrow topography is considerably more beneficial to converting energy from barotropic to baroclinic fields than low and wide topography. Furthermore, parameter s, which is an important non-dimensional parameter for internal tide generation, is not the sole parameter by which the baroclinic velocity fields and conversion rate are determined.  相似文献   

19.
Wind erosion, or the transportation and deposition of sand into desert dunes and aeolian loess, is one of the most important aeolian activities. The progression of aeolian landforms expands arid and barren landscapes, leading to the degradation of adjacent areas. The Gonghe Basin, as a typical plateau with abundant sand sources, is highly sensitive to changes in the local climate conditions. In order to quantify the spatial-temporal variations in the aeolian landforms in the Gonghe Basin, we conducted field surveys and also analyzed twelve remote sensing(Landsat5 TM and Landsat8 OLI) images that sample the Gonghe Basin from 1989 to 2019. In the Gonghe Basin, we identified aeolian landforms such as climbing dunes on the windward slopes of the foothills, checkerboard dunes in the southeastern part of the basin, flat dunes, parabolic dunes and crescent dunes on the east and west sides of Longyangxia Reservoir, shrubby sandbanks on the valley slope in Shazhuyu, Tanggemu, and Indel, and sandy thickets at the bottom of the valley near the Dalian Sea, the Longyangxia Reservoir, and the tributaries of the Yellow River. From 1989 to 2005, the area of theaeolian regions expanded by 816.7 km~2, with an annual conversion rate of 0.05%. From 2015 to 2019, the area of the aeolian regions shrunk by 2411.9 km~2, with an annual conversion rate of-0.15%.The number and size of the fixed and semi-fixed dunes(e.g. the shrubby sandbanks on the valley slope and the sandy thickets at the bottom of the valley) were more stable than those of the mobile dunes(e.g. the checkerboard dunes, the flat dunes, the crescent dunes, the parabolic dunes, and the climbing dunes). The fixed and semi-fixed dunes were arranged in an irregular ring shape, and the location of the center of gravity of this ring did not change significantly from 1989 to 2019; in this time, the mobile dunes migrated to the northwest.  相似文献   

20.
以鱼粉和酪蛋白为蛋白源,添加鱼油、豆油、α-马铃薯淀粉、纤维素以及适量的维生素和矿物质配制成实验饲料,蛋白质质量分数设置41%、47%、53%3个水平,能量设置12 561、14 655、16 748 kJ/kg 3个水平,饲养点带石斑鱼幼鱼59 d后,以增重率、蛋白质效率、饲料系数为评价指标,分析不同能量蛋白比的饲料对点带石斑鱼体内消化酶活性的影响。结果表明:当能量蛋白比分别为31.30、33.50、32.26 kJ/g时,点带石斑鱼增重率、蛋白质效率达到极大值,饲料系数达到极小值;能量蛋白比在31.62~35.54 kJ/g范围内,胃、肠道的蛋白酶、淀粉酶、脂肪酶活性较强。点带石斑鱼幼鱼配合饲料中合适的能量蛋白比为31.62~33.50 kJ/g。  相似文献   

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