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1.
ＩＮＴＲＯＤＵＣＴＩＯＮＡｍｐｈｉｏｘｕｓｏｒｌａｎｃｅｌｅｔ,ａｃｅｐｈａｌｏｃｈｏｒｄａｔｅ,ｈａｓｌｏｎｇｂｅｅｎｒｅｇａｒｄｅｄａｓｔｈｅｌｉｖｉｎｇｉｎｖｅｒｔｅｂｒａｔｅｍｏｓｔｒｅｓｅｍｂｌｉｎｇｔｈｅａｒｃｈｅｔｙｐｅｏｆｖｅｒｔｅｂｒａｔｅｓ.Ｉｔｈａｓｂｅｅｎｗｉｄｅｌｙｋｎｏｗｎａｓｏｎｅｏｆｔｈｅｍｏｓｔｉｍｐｏｒｔａｎｔｓｐｅｃｉｅｓｉｎｔｈｅｚｏｏｌｏｇｉｃａｌｗｏｒｌｄ,ａｎｄｈａｓｂｅｅｎｅｍｂｒｙｏｌｏｇｉｃａｌｌｙｓｔｕｄｉｅｄｆｏｒｏｖｅｒ130ｙｅａｒｓ(Ｚｈａｎ… 相似文献
2.
Heat shocks effectively produced triploids inPenaeus chinensis. Fertilized eggs heat shocked (28–32°C) for 8 to 16 minutes, starting from 8 to 20 minutes after spawning, resulted in triploidy
induction rates of 39%–75%. Several triploid populations were cultured to 10 cm. In a triploid population, two kinds of ovaries
were observed. Histological examination showed apparent differences between these two kinds of ovaries, whereas among male
shrimp, there were no such differences.
Contribution No. 3465 from the Institute of Oceanology. Chinese Academy of Sciences.
The study was supported by the International Foundation for Science, Asian Fisheries Society and Chinese Climbing Project
PDB 6–2, and Chinese National 863 Project (No. 863-819-01-05). 相似文献
3.
Induced nest spawning and artificial hatching of the fertilized eggs of mudskipper, Boleophthalmus pectinirostris 总被引：2，自引：0，他引：2
In this study, nest spawning was successfully induced by exogenous hormone injections and seawater flow stimulation, and optimum condition for hatching fertilized eggs of burrow fish mudskipper,Boleophthalmus pectinirostris, was searched. Apart from spawning inside the nests, females also spawned outside the nests. The percentages of spawned nests were 8.0% to 24.2%. Most eggs were observed adhered to the inner wall of the top half of the nest. Fertilization rates of the nest-spawned eggs varied from 17.3% to 80.8% Females could spawn after being artificially confined inside the‘nests with males at ratios of 1:1 or 1:2, but the spawned eggs were not fertilized. Mean hatching rates of artificially fertilized eggs incubated in round plastic buckets were 32.7%-70.6%, and in the net cages, were 4.2%-20.5%, respectively. Mean hatching rates of nestfertilized eggs incubated in the round plastic buckets were 33.6%-76.3%, and in the net cages, were 5.9%-25.2%.Results showed that round bucket incubation was the best way for hatching fertilized eggs of mudskipper.Keeping the hatching seawater flowing is an important way for increasing the hatching rates of the mudskipper fertilized eggs. 相似文献
4.
Mature amphioxus (Branchiostoma belcheri tsingtauense) collected by trawl in June, 1994 from Qingdao Shazikou sea area bottom sands, were cultured under controlled indoor conditions, where they spawned and their eggs were fertilized and hatched into larvae which gradually settled at the bottom and dug into the sands after 40-50 days culture in a water trough outdoors. The total survival rate of the larvae(LSR)was 5.5% before they went into the sands, 1.9% in 4 months, 0.7% in 10 months. The survival rate of the young fish which had dug into the sands (YSR) was 35.6% in 4 months, and 12.6% in 10 months. The amphioxus number tended to be constant from the 11th month on. In the first five months after incubation, the amphioxus body length increased by an average of about 1.5 mm a month, and about 0.4 mm a month from Dec. to May of next year. The maximum length after ten months was 24 mm; the average was about 11 mm. After two years culture in the water trough, the maximum length could reach 34-35 mm, when the amphioxus gonads began to develop. 相似文献
5.
Triploidy was induced in Chinese shrimpFenneropenaeus chinensis by 30±0.5°C heat shock treatment (initiated at 20 min after fertilization) for 10 min to inhibit the release of PB2 at 18.0°C.
The highest triploid rate obtained was 84.5% in nauplius stage. The effect of heat shock treatment on meiosis and cleavage
of eggs was investigated in this work aimed to establish efficient procedures for triploid induction and to gain understanding
of the mechanism of triploid production. Three pronuclei that could be observed in the treated eggs under fluorescence microscope
developed into triploid embryos. Some abnormal chromosome behavior was observed in heat shocked eggs.
Contribution No. 4407 from the Institute of Oceanology, Chinese Academy of Sciences.
This work was supported by Project under Major State Basic Development Program (G1999012009) and Knowledge Innovation Program
of the Chinese Academy of Sciences (KZCX 2-211) 相似文献
6.
In this study, nest spawning was successfully induced by exogenous hormone injections and seawater flow stimulation, and optimum
condition for hatching fertilized eggs of burrow fish mudskipper,Boleophthalmus pectinirostris, was searched. Apart from spawning inside the nests, females also spawned outside the nests. The percentages of spawned nests
were 8.0% to 24.2%. Most eggs were observed adhered to the inner wall of the top half of the nest. Fertilization rates of
the nest-spawned eggs varied from 17.3% to 80.8%. Females could spawn after being artificially confined inside the nests with
males at ratios of 1∶1 or 1∶2, but the spawned eggs were not fertilized. Mean hatching rates of artificially fertilized eggs
incubated in round plastic buckets were 32.7%–70.6%, and in the net cages, were 4.2%–20.5%, respectively. Mean hatching rates
of nest-fertilized eggs incubated in the round plastic buckets were 33.6%–76.3%, and in the net cages, were 5.9%–25.2%. Results
showed that round bucket incubation was the best way for hatching fertilized eggs of mudskipper. Keeping the hatching seawater
flowing is an important way for increasing the hatching rates of the mudskipper fertilized eggs.
Supported by the NSFC (No. 30170739), and also supported by the Provincial Natural Science Foundation of Fujian Province (No.
B0210003). 相似文献
7.
《中国海洋湖沼学报》2017,(6)
The reproductive biology of bream A bramis brama(L.) was studied from 546 fish collected from the lower reaches of the Irtysh River in Xinjiang, north-west China, from March to November 2013. The overall sex ratio(M/F) was 1.06:1, and was not significantly diff erent from the theoretical 1:1 ratio( P 0.5). However, there was a dominance of males during the pre-spawning season(1.33 in March and 1.56 in April, P 0.5), while females were dominant during the peak spawning season(0.88 in June, P 0.5). The monthly variation in gonadosomatic index(GSI) and proportion of gonads at each macroscopic maturity stage, indicated that A. brama spawned once a year with peak spawning occurring from late May to June. The unimodal distribution of oocyte diameter each month indicated that A. brama is a single spawner, with a high degree of spawning synchronicity. The standard lengths(SL 50) and ages( A 50) at first maturity for males and females, were 178 and 204 mm, and 5.6 and 6.8 years, respectively. The mean absolute fecundity(AF) was 77 311 eggs per fish, and mean relative fecundity(RF) was 162 eggs per gram of body weight(BW). The AF of A. brama increased linearly with increasing of gonad weight(GW), eviscerated weight(EW) and standard length(SL), but was not significantly correlated with age. As, A. brama in the lower reaches of the Irtysh River reaches reproductive maturity relatively late in their life span, is mature for a short period and spawns in aggregations, this fish is vulnerable to overexploitation. 相似文献
8.
Tetraploid induction was carried out by inhibiting mitosis I in fertilized eggs ofChlamys farreri. Mitosis I was blocked with cold shock (5–7°C), Cytochalasin B (0.75 mg/L) and 6-dimethylaminopurine (6-DMAP) (60–75 mg/L)
when 60% fertilized eggs released polar body II at 20°C. At 4-cells embryo stage, the ploidy was determined by counting chromosome
number. In control groups, most embryos were diploids (72.22%) and aneuploids (24.78%). In Cytochalasin B, cold shock and
6-DMAP treated groups, tetraploids were respectively 10.51%, 4.08%, and 13.34%; aneuploids were 43.10%, 35.93% and 29.16%,
and triploids were 7.84%, 8.52% and 18.33%. At D-larva stage, ploidy was determined by flow cytometry (FCM). The ploidy analysis
of day 2 larvae showed diploids in control group and also in three treated groups. Juvenile scallops (0.2–0.3cm) which were
harvested in two control groups and two CB treated groups were all diploids through checking ploidy individually by FCM.
Contribution No. 3648 from the Institute of Oceanology, Chinese Academy of Sciences.
Contribution No. 238 from the Experimental Marine Biology Laboratory, Chinese Academy of Sciences.
Project funded by grant (No. 819-01-07) from the Chinese Science and Technology Ministry and also by “Hundred Talents Plan”
grant from the Chinese Academy of Sciences. 相似文献
9.
以综合催熟措施和移植精荚手术相结合的技术方法，解决了鱼塭产斑节对虾催熟效应期长，产卵率低，易蜕壳失去精荚等难题。使用 ２７８ 尾雌虾，催熟率达９０％ 以上，在４０ ｄ 内共产卵６０８ 尾次，卵量１８ ５３０ 万粒，孵出无节幼体１１ ９９８ 万尾，平均孵化率 ６４．７％ ，平均每尾雌虾孵出无节幼体 ４３．１ 万尾，育成商品虾苗 １ ０２８．５ 万尾，育苗平均成活率 １３．６％ ，试养成活率５７．１％ 相似文献
10.
STUDY ON MIXIS POTENTIAL OF ROTIFER RESTING EGGS（ BRACHIONUS PLICATILIS ） WITH DIFFERENT COLLECTION TIMES AND DIFFERENT PRESERVATION PERIODS 总被引：1，自引：0，他引：1
The present study investigated the possible changes in the mixis potential of rotifer resting eggs produced by a single stock of Brachionus plicatilis and collected and preserved annually from 1985 - 1998. Several clones derived from each batch of resting eggs were cultured under the same conditions for 21 days. The percentage of clones appearing resting eggs and the average yield of resting eggs produced from each clone were recorded and statistically analyzed to find the differences between the mixis potential of those resting egg batches. Results showed that different batches of resting eggs had different mictic levels among their descendent clones; but no regular relationship was found between the mixis potential of resting eggs and their collection times/preservation periods. Several internal and external factors that might affect the mixis potential of resting eggs were discussed. 相似文献
11.
The present trial was conducted with starry fl ounder Platichthys stellatus broodstock to evaluate the effects of dietary vitamin A,ascorbic acid,and α-tocopherol on the gonadal development and reproductive performance.8 000 IU/kg diet vitamin A(VA group),500 mg/kg diet ascorbic acid(Vcpp group),or 250 mg/kg diet α-tocopherol(α-TA group)was added into basal diet to create 3 vitamin experimental diets,respectively.Each diet was fed to 450 starry fl ounder broodstock for 104 days.Samples were collected weekly.The gonadosomatic index(GSI)of 3 vitamin experimental groups fi rst decreased and then increased.Maximum GSI of Vcpp group was higher than that of α-TA group but lower than that of VA group.The spawning periods of 3 vitamin experimental groups lasted 49,56,and 45 days,respectively.No mature eggs were observed in the control group during the trial.The absolute fecundity(AF)and relative fecundity(RF)of α-TA group was higher than that of Vcpp group but lower than that of VA group.The results suggest that different vitamins play different roles in the fi sh reproductive process.Vitamin A stimulated the maturation of the ovary,ascorbic acid prolonged the spawning period,and α-tocopherol affected the development of the eggs. 相似文献
12.
The effects of 60-day starvation on survival rate. condition index (CI) , changes ofnutrient composition of different tissues, respiration and excretion of scallop ChlartnCs farreri were studiedin laboratory from Oct. 17 to Dec. 15 ,1997. Two groups (control and starvation with 200 individualseach) were cultured in two 2 m3 tanks, with 31 to 32 salinity water at 1 7 “C . Starvation effects were mea-sured alter 1 0, 20, 40 and 60 days. There was no mass mortality of scallops cf the two tanks and survivalrates of the control and starvation groups were 93 .5 % and 92.0 %, respectively. Starvation had strongeffect on the meat condition of the scallops, especially after 10 days; when relative lipid percentagedropped sharply while relative protein percentage increased. The impact of starvation on the oxygen consumption mtc (OCR) and the ammonia-N cxcretion mtc (AER) was obvious. 相似文献
13.
The limited knowledge about the relationships between environmental factors and the maturation and reproductive seasonality of nemerteans is mostly obtained from field studies, and in many cases is conjectural. Few species have been studied under well-controlled laboratory conditions. In this work, the effects of temperature and photoperiod on gonad development and spawning of Cephalothrix hongkongiensis were observed by rearing worms under different temperatures and photoperiods. Worms reared at 4℃ and 6℃ showed no growth or gonadal development, and all died within 109 days. At 8℃ and 12℃, gonads could be seen from 90 days to the termination of the 360-day experiment, but no spawning was observed. Worms maintained at 16, 24, and 28℃ showed both gonadal development and spawning, and matured faster at higher temperatures. Under a fixed temperature(16℃), photoperiod did not have any significant impact on maturation and spawning. It is likely that temperature plays a major role in regulating the maturation and reproductive cycle of C. hongkongiensis. 相似文献
14.
SEAWEED CULTIVATION: TRADITIONAL WAY AND ITS REFORMATION 总被引：4，自引：0，他引：4
INTRODUCTIONSeaweedcultivationisthemosteffectivewayforobtaininglargeamountofseaweedproducts.Thoughseaweedcultivationhasahistoryofonlyafewdecades,itsdevelopmentisfast (Fei,1 996) .Thetotalproductionofcultivatedseaweedatpresentisabout 62 50× 1 0 3tonsfreshweight,ofwhichLaminariaaccountsfor 3 1 0 0× 1 0 3tons,Undaria 2 2 0 0x 1 0 3tons,Porphyra 750× 1 0 3tons,Gracilariasp .1 0 0× 1 0 3tons,EucheumaandKappaphycus 1 0 0× 1 0 3tons,otherkindofseaweeds 1 0 0× 1 0 3tons.Theestimatedtotal… 相似文献
15.
《中国海洋大学学报(英文版)》2015,(6)
We applied solution-based ICP-MS method to quantify the trace-elemental signatures in statoliths of jumbo flying squid, Dosidius gigas, which were collected from the waters off northern and central Chile during the scientific surveys carried out by Chinese squid jigging vessels in 2007 and 2008. The age and spawning date of the squid were back-calculated based on daily increments in statoliths. Eight elemental ratios(Sr/Ca, Ba/Ca, Mg/Ca, Mn/Ca, Na/Ca, Fe/Ca, Cu/Ca and Zn/Ca) were analyzed. It was found that Sr is the second most abundant element next to Ca, followed by Na, Fe, Mg, Zn, Cu, Ba and Mn. There was no significant relationship between element/Ca and sea surface temperature(SST) and sea surface salinity(SSS), although weak negative or positive tendency was found. MANOVA analysis showed that multivariate elemental signatures did not differ among the cohorts spawned in spring, autumn and winter, and no significant difference was found between the northern and central sampling locations. Classification results showed that all individuals of each spawned cohorts were correctly classified. This study demonstrates that the elemental signatures in D. gigas statoliths are potentially a useful tool to improve our understanding of its population structure and habitat environment. 相似文献
16.
The present paper reports 7 species of pontoniine shrimps collected from Hainan Island,South China Sea,by the Joint Chinese-German Marine Biology Expeditions,in which,Conchodytes nipponensis(De Haan,1844)and Coralliocaris brevirostris Borradaile,1898 are new records from Chinese waters. 相似文献
17.
The Al—basic sacrificial anode is widely used to protect steel construction from corrosion in seawater, but is not suitable
for protecting steel in freshwater. In the estuary area, seawater and freshwater mix and different seawater salinity can be
formed in different mixing sites between freshwater and seawater. Based on the requirements of the Shengli Petroleum Administrative
Bureau of China, the effect of seawater salinity on electrochemical properties of Al—basic anode was studied under laboratory
conditions by the method given in National Standard GB 4948-85 (China Starndard Bureau, 1985). No obvious effect was found
when seawater salinity was 30 to 10, but obvious effect on anodic open circuit potential, closed circuit potential and current
efficiency was found when seawater salinity was 5. These values are lower than those given in the National Standard.
Contribution No. 2681 from the Institute of Oceanology, Chinese Academy of Sciences. This study is a major project (Ky85-11-5)
and a key project (A14920416) of the Chinese Academy of Sciences, and also received support from the Shengli Oil Field. 相似文献
18.
Localization of a putative epiboly-determining factor in oocytes of the goldfish (Carassius auratus)
It is unknown whether cytoplasmic determinants in goldfish eggs are asymetrically localized before maturation. In this study
fully grown goldfish oocytes with intact germinal vesiles (GVs) were ligated with baby hair loops along desired planes into
two parts, matured in vitro, and inseminated. Embryos developed from the animal halves with GV of oocytes ligated along the
equator were unable to undergo epiboly, while those developed from the GV-containing parts of oocytes ligated along the animal-vegetal
axis were able to. Also, embryos developed from the animal halves obtained from fertilized eggs could undergo epiboly. From
these results, it was postulated that the goldfish's premature oocyte possessed a putative factor in the vegetal hemisphere
which was responsible for epiboly of the embryonic cells.
This work was supported by the NSFC and the “Climb Plan B” (PD-B6-1-1) of the Chinese Commission of Science and Technology.
Contribution No. 2915 from the Institute of Oceanology, Chinese Academy of Sciences. 相似文献
19.
In this paper, the morphological characters of eggs and larvae ofSardinella aurita (Cuvier & Valenciennes), its spawning ground, spawning seasons and spawning conditions have been studied.
Sardinella aurita likes to breed in the upwelling area and selects the Waixie fishing ground as its main spawning ground. The months from February
to September are its spawning seasons, reaching its peak in April. In the main spawning ground, the temperature of the surface
layer was found to be 24.4–25.2°C, the salinity 33.87–34.07%. and the depth of water between 34–60m.
The distribution of the larvae is closely related to the distribution of plankton, the path of migration of adult fish, and
the current direction of the water system.
In order to protect fishery resources, it is necessary to prohibit catching the spawning fish in the Waixie fishing ground
in April, and the catching of immatures from March to June.
This paper was published in Chinese inOcean. Limn. Sinica
14(3): 240–248, 1983. 相似文献
20.
A new species of cymothoid Isopeda, Nerocila donghaiensis, collected from the body of a sciaenid fish, Johnius sp., is described in the present paper. The new species can be distinguished from the other species of genus Nerocila by its endoped of uropods with deep notch, sharply serrated on the lateral margin and strongly serrated on the medial margin; antennule with 1 st article swollen; dactyli of pereopods Ⅰ, Ⅱ, Ⅳ, Ⅴ swollen. 相似文献