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1.
Studies were carried out in the Ob River estuary and at the adjacent shelf of the Kara Sea. The concentrations of organic carbon, lipids, major elements (Na, Mg, P, S, K, and Ca), trace elements (Li, Be, B, Al, Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, As, Se, Rb, Sr, Y, Mo, Ag, Cd, Sb, Cs, Ba, Hg, Tl, Pb, Bi, Th, and U), and rare-earth elements (La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, and Lu) were determined in the dominant species of mesozooplankton (Senecella siberica, Limnocalanus macrurus, and Calanus spp.). The similarities and differences are shown for the chemical compositions of the specimens. Calanus spp. are characterized by a large Li accumulation with concentrations ~350 times higher than those in S. siberica and L. macrurus. The total accumulation of chemical elements per unit volume is higher in L. macrurus than in S. siberica and Calanus spp., amounting to 6.63, 0.69, and 0.41 mg, respectively. The intensity of biological accumulation and the spatial disposition of the area of maximum accumulation of elements in the zooplankton community within the boundaries of the Ob River estuary depend on the hydrophysical conditions. Postmortem variations in the concentrations of chemical elements in dead L. macrurus are characterized by a multidirectional nature. The revealed distinctions of the chemical compositions in live and dead L. macrurus represent the features of lifetime and postmortem concentrations of elements.  相似文献   

2.
Passive sampling could provide the solution to problems associated with costly and time consuming sampling programmes and biomonitoring. Mussels (Mytilus edulis) and Chemcatcher passive sampler were simultaneously analyzed for sequestered pesticides and polychlorinated biphenyls (PCBs) by gas chromatography with mass spectrometry (GC/MSD). The Chemcatcher passive sampler based on a solid phase extraction disc with two types of diffusion-limiting membranes (polyethelene and polysulphone), were also compared. Diuron, atrazine, irgarol and lindane were accumulated in the polysulphone passive sampler in greater concentration than in the mussels or in the other passive samplers with polyethelene limiting-membrane. Mussels can accumulate in their tissue high concentration of non polar compounds such as PCB 52, dieldren and PCB 153; more than the passive sampler with polysulphone membrane. The device with polyethelene limiting membrane has high affinity capacity to concentrate high amount of phenenthrene, dieldrin, PCB 153 and PCB 52, so it acts as a sampler for non-polar compounds. The highest uptake rate of hydrophobic compounds by Chemcatcher was observed for analytes with log octanol-water partition coefficient (KOW) between 4.5 and 7.5. Laboratory-based studies using passive samplers to assess the potential for bioaccumulation could provide robust and reliable information at relatively low cost compared to biomonitoring data. Laboratory data obtained using passive samplers could be related to accumulation under field conditions where field assessments are required.  相似文献   

3.
The spatial variability of chlorophyll a concentrations was studied from the data of two near-shore expeditions and the cruise of the R/V Akademik M.A. Lavrent’ev in October–November 2010 over the northwestern part of the Sea of Japan. The sections across eddies showed a maximum of chlorophyll a at a depth of 40 m. According to the data from the cruise, the chlorophyll a concentration was maximum in the north of the sea and decreased to the south. In parallel, the procedures for chlorophyll a determination were compared for spectrophotometry with a fluorescence probe and a fluorescence flow system. The probe data of chlorophyll a fluorescence showed a high correlation with the chlorophyll a concentrations by spectrophotometry. On the contrary, data on chlorophyll a concentrations from spectrophotometry did not agree with those from the flow system. It was shown that a fluorimeter in the flow system recorded dissolved organic matter along with the chlorophyll a fluorescence.  相似文献   

4.
In 2012, expeditions of the Institute of Microbiology, Russian Academy of Sciences, delivered samples of algo-bacterial mats from Kulunda steppe alkaline lakes (Petukhovskoe alkaline lake, Tanatar VI, and Gorchina III). The filamentous alga Ctenocladus circinnatus (Chlorophyta) acted as an edificator of the mats. The composition of cenoses algocomponents also included chlorophytes Dunaliella viridis and Picocystis salinarum as well as diatoms Anomeoneis sphaerophora, Brachysira brebissonii, B. zellensis, Mastogloia pusilla var. subcapitata, Nitzschia amphibia, N. cf. communis, and Nitzschia sp. 1. The composition and structure of phototrophic algae cenoses (including diatom taxocenes) were described for the investigated lakes for the first time. For the period from 2011 to 2012, the total mineralization significantly increased in lakes. This involved sensible alterations of cenoses. B. zellensis was the most permanent component of diatom taxocenes in both seasons. In the summer of 2011, it was often accompanied by A. sphaerophora and B. brebissonii. In the summer of 2012, A. sphaerophora was found only singularly in Lake Gorchina III, and some biotopes of Lake Tanatar VI were massively inhabited by N. cf. communis, including colonies that had not been previously described for the species. The genetic analysis of three diatoms, which are markedly different from each other in their appearance and were sampled from different lakes but were all determined as Nitzschia cf. communis, showed their complete similarity to each other with the 18S rRNA gene fragment and the highest similarity of all the three diatoms with the species Nitzschia communis.  相似文献   

5.
Little is known regarding how harmful algal bloom species respond to different temperatures in terms of fatty acid production. This study examined the effects of temperature on the growth rates, cell volumes, and fatty acid concentrations and compositions of four harmful algal bloom species (HABs), Akashiwo sanguinea, Alexandrium tamarense, Chattonella ovata, and Prorocentrum minimum. The HABs species were cultured at 15, 20, 25, and 30°C in a nutrient-enriched medium. Three of the species maintained optimal growth rates over a wide range of temperatures, but A. tamarense did not. The cell volumes of each species showed little change over the temperature range. The total fatty acid concentrations in A. sanguinea, A. tamarense and C. ovata decreased as the temperature increased, but P. minimum showed no trend in this respect. Polyunsaturated fatty acids (PUFAs), the key biochemical components that maintain cell membrane fluidity and which are associated with toxicity, decreased in both concentration and proportion of total fatty acids as temperature increased, except in A. sanguinea, in which the proportion of PUFAs to the total fatty acids increased. These reductions in PUFA concentration and proportion could reduce cell membrane fluidity and toxicity in HABs; however, enhanced growth and/or ruptured cells, which are considered more toxic than intact cells, could compensate for the reduced per-cell toxicity. This phenomenon might impact on the marine ecosystem and aquaculture industry.  相似文献   

6.
认识海洋在全球碳循环中的作用及其对环境变化的响应,需要高时空分辨率的观测数据。由于轨道宽度、云雨天气、太阳耀斑等的影响,单一的水色传感器的观测能力十分有限,将多源海洋水色卫星进行融合是提高水色数据时空覆盖的一种有效途径。SeaWiFS和MERIS分别于2010年12月11日和2012年5月9日停止运行,在很大程度上降低了水色融合产品时空覆盖的提升。我们在融合过程中加入了FY-3 MERSI数据,生成了全球海洋叶绿素浓度遥感融合产品数据集。数据源包括SeaWiFS、MERIS、MODIS-Aqua、VIIRS和MERSI。结果表明：加入MERSI后,融合产品的日平均有效空间覆盖提高了9%;采样频率（同一区域一年中获取有效数据的次数）由57天/年提高到109天/年。利用实测数据和国外同类融合产品（ESA GlobColour和NASA MEaSUREs）对新的数据集进行了质量评价。与实测数据相比,加入MERSI的融合产品精度与未加入MERSI的融合产品基本一致;与国外同类融合产品的偏差小于10%。新数据集的时间序列特性与未加入MERSI的融合产品以及单传感器的一致。  相似文献   

7.
Based on long-term (1985–1995) monitoring data, the paper considers the peculiarities of seasonal variability in the spatial and vertical distribution of particulate organic phosphorus (РPOM) in the surface layer and in the photosynthetic zone in the northwestern Black Sea. Regression equations, experimental data, and satellite observations for the chlorophyll a concentration allowed us to evaluate the seasonal longterm (1979–1995) variability in РPOM in the surface layer and photosynthesis zone. The ratios of the concentrations of particulate organic carbon, nitrogen, phosphorus, and chlorophyll a are calculated and statistical estimates of seasonal changes in the РPOM in the areas with different degrees of influence of river runoff and water of open seas are obtained. The consistency of intra-annual changes in the concentrations of РPOM, chlorophyll a, and phytoplankton biomass is shown, which indicates the role of phytoplankton in the formation of РPOM and in its intra- and interannual variability in the northwestern part of the sea. It is shown that long-term seasonal variations in РPOM and related changes in the concentration of chlorophyll a depend on the variability of bulk river runoff, the extent of its abundance in the northwestern shelf, and regional hydrometeorological conditions.  相似文献   

8.
创伤弧菌是一种可感染人类的河口病原菌。建立快速,特异而敏感的检测方法,有助于创伤弧菌感染的早期疾病诊断和及时治疗。本研究设计了针对vvhA基因的一系列引物（包括两对外部引物和两对内部引物）,采用环介导等温扩增技术（LAMP）来检测创伤弧菌。结果显示,本方法的最适扩增温度是63℃,反应仅需35分钟。扩增产物不仅可以用含有DNA ladder的琼脂糖凝胶电泳检出,也可借助钙黄绿素直接肉眼观察。采用45株菌株检测该方法的特异性,其中所有创伤弧菌均被检出而其他菌株检测结果皆为阴性。本方法的敏感性是普通PCR扩增的100倍,同时利用该方法可以准确检测出所有的模拟样品、临床标本及环境样品。与其他已知方法比较,针对vvhA基因的介导等温扩增技术可以快速、简单、敏感及特异地鉴定创伤弧菌。  相似文献   

9.
Time series changes in sea surface temperature (SST), chlorophyll a (Chl a), nutrients (PO4, NO3), and sea winds, which correlated with the passage of Typhoon Shanshan in the East/Japan Sea (EJS), are illustrated using satellite data for Chl a, SST, sea winds, and in situ data for nutrients and water temperature. The sea-surface cooling (SSC) effect by the passage of the typhoon was higher at stations nearer to the center compared to stations further from the center. The SSC effect at stations in the colder water region (on the left side of the typhoon’s track) was higher than at stations in the Tsushima Warm Current region (on the right side of the typhoon). The SSC effect continued for approximately 10 days after the passage of the typhoon. The Chl a concentration at all stations increased after the passage of the typhoon. This increase continued for a period of approximately 10 days, but the duration period at each station varied with distance from the typhoon center. Changes in Chl concentrations at stations within a 2° distance on both sides from the typhoon’s center were higher than that at other stations. The changes in Chl a by the passage of the typhoon were measured at approximately 0.3–1.0 mg/m3 along the moving path of the typhoon. Phosphate and nitrate changes were inversely correlated with the water temperature changes; the nutrient concentration increased with the passage of the typhoon. Like the changes in SST, changes in nutrient concentrations on the left side of the typhoon’s track were higher compared to those at the center and the right side.  相似文献   

10.
We investigated the relationship between chlorophyll a (Chl-a) concentrations estimated from satellite observations and the activity of eddies in the Kuroshio Extension region. High (low) area-averaged Chl-a concentrations were frequently observed in the core of cyclonic (anticyclonic) eddies. Such relationships between Chl-a concentrations and eddy cores were not frequently observed in the southern part of the recirculation gyre, and advection of background meridional gradient of Chl-a by eddy-edge currents accounted for Chl-a spatial variability. Decadal-scale changes of Chl-a concentrations around the Kuroshio Extension were strongly affected by eddy activity and transport but not by large-scale near-surface isopycnal heaving. We also found that decadal changes of nutrient concentrations near the main stream could affect Chl-a concentrations in the southern part of the recirculation gyre via southward transport of eddies and mean flow.  相似文献   

11.
河口有色溶解有机物（colored dissolved organic matter,CDOM）的分布是各种物理-生物地球化学过程共同作用的结果。为实现河口高动态变化CDOM的监测,遥感是一种重要的手段。由珠江口四个不同季节的航次获得的实测数据,本文构建了一个遥感算法以反演CDOM在400 nm的吸收系数（aCDOM （400））。该算法使用以波段反射率比值Rrs （667）/Rrs （443）和Rrs （748）/Rrs （412）为自变量。将构建的算法应用于2002-2014年的MODIS/Aqua数据,本文计算了珠江口不同季节的aCDOM （400）气候态分布。CDOM的分布主要受珠江径流量和区域水下地形特征的影响。沿着垂直于水深梯度的断面,气候态aCDOM （400）呈指数减少（y=aebx,b<0）,但不同季节差异很大。珠江口CDOM主要是河流淡水输运而来。其中,富里酸比例随盐度的增加而降低。基于构建的算法、CDOM保守混合方程和径流量,本文由MODIS/Aqua数据进一步估算了2002-2014年夏季和冬季珠江DOC的有效入海浓度和有效入海通量。珠江的有效入海浓度和有效入海通量都与流量存在正相关关系,且在夏季的相关性更明显,R2分别为0.698和0.9657。  相似文献   

12.
The frequency spectrum of surface elevations in the presence of wind waves is well known. On this basis, one can estimate the frequency spectrum of vertical velocities in sea-surface waves. Owing to liquid incompressibility, the spectrum of horizontal velocities should have the same frequency dependence. The use of the dispersion equation for waves on the surface of a heavy liquid allows one to obtain to the spatial spectrum of velocities. Therefore, one can estimate the spatial structure function of the velocity field. For short waves and large depths, the structure function increases as r 1/2, where r is the distance between the points of observations. For long waves and shallow depths h, this increase is proportional to r. The coefficient of turbulent mixing K(r) of pollution spots of size r on the sea surface is now estimated as the product of the spot size and the rms difference of velocities. As a result, depending on r and h, the exponent in the r n dependence of K(r) may vary between 1.25 and 1.5. This outcome provides an explanation for a scatter in the values of the exponent n, a phenomenon that has been observed by many experimentalists.  相似文献   

13.
This study investigated alkaline phosphatase (APase) activity and dissolved organic and inorganic phosphorus utilization by the harmful dinoflagellate Gymnodinium impudicum (Fraga et Bravo) Hansen et Moestrup isolated from the South Sea of Korea. Under conditions of limited phosphorus, observation of growth kinetics in batch culture yielded a maximum growth rate (μmax) of 0.41 /day and a half saturation constant (Ks) of 0.71 μM. In time-course experiments, APase was induced as dissolved inorganic phosphorus (DIP) concentrations fell below 0.83 μM, a threshold near the estimated Ks; APase activity increased with further DIP depletion to a maximum of 0.70 pmol/cell/h in the senescent phase. Thus, Ks may be an important index of the threshold DIP concentration for APase induction. G. impudicum utilizes a wide variety of dissolved organic phosphorus compounds in addition to DIP. These results suggest that DIP limitation in the Southern Sea of Korea may have led to the spread of G. impudicum along with the harmful dinoflagellate Cochlodinium polykrikoides in recent years.  相似文献   

14.
As a major aldehyde pollutant widely existing in industry and our daily life, acetaldehyde is more and more harmful to human health. As characteristic habitat niche, bacteria from deep sea environments are abundant and distinctive in heredity, physiology and ecological functions. Thus, the development of acetaldehyde-degrading bacteria from deep sea provides a new method to harness acetaldehyde pollutant. Firstly, in this study,acetaldehyde-degrading bacteria in the deep sea water of the West Pacific Ocean were enriched in situ and in the laboratory respectively, and then the diversity of uncultured bacteria was studied by using 16 S r RNA genes. Then acetaldehyde-degrading strains were isolated from two samples, including enrichment in situ and enrichment in laboratory samples of deep sea water from the West Pacific Ocean using acetaldehyde as the sole carbon source,and then the ability of acetaldehyde degradation was detected. Our results showed that the main uncultured bacteria of two samples with different enrichment approaches were similar, including Proteobacteria,Actinobacteria, Firmicutes, Cyanobacteria, but the structure of bacterial community were significant different.Four subgroups, α, γ, δ and ε, were found in Proteobacteria group. The γ-Proteobacteria was dominant(63.5%clones in laboratory enriched sample, 75% clones in situ enriched sample). The species belonged to γ-Proteobacteria and their proportion was nearly identical between the two enrichment samples, and Vibrio was the predominant genus(45% in laboratory enriched sample, 48.5% in situ enriched sample), followed by Halomonas(9% in situ enriched sample) and Streptococcus(6% in laboratory enriched sample). A total of 12 acetaldehyde-degrading strains were isolated from the two samples, which belonged to Vibrio, Halomonas,Pseudoalteromonas, Pseudomonas and Bacillus of γ-Proteobacteria. Strains ACH-L-5, ACH-L-8 and ACH-S-12,belonging to Vibrio and Halomonas, have strong ability of acetaldehyde degradation, which could tolerate 1.5 g/L acetaldehyde and degrade 350 mg/L acetaldehyde within 24 hours. Our results indicated that bacteria of γ-Proteobacteria may play an important role in carbon cycle of deep sea environments, especial the bacteria belonging to Vibrio and Halomonas and these strains was suggested for their potentials in government of aldehyde pollutants.  相似文献   

15.
To understand the temporal and spatial variation of the prokaryotic community in the East Sea, their composition was determined by polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE)-sequencing techniques. The investigations were conducted twice annually in 2007 and 2009 in coastal and offshore stations. Prokaryotic abundance (PA), leucine incorporation rate, and other environmental parameters were also measured. By using the DGGE approach, we obtained 283 bacterial sequences and 160 archaeal sequences. The most frequently detected bacterial phylotypes during the investigations belonged to Alphaproteobacteria, Gammaproteobacteria, and Bacteroidetes. However, their relative compositions differed in time and space. Although Alphaproteobacteria and Bacteroidetes were the dominant groups in the surface water in May 2007 and in May and October 2007, Gammaproteobacteria was dominant in mesopelagic samples. However, Gammaproteobacteria was overwhelmingly dominant in most samples in August 2009. Although Deltaproteobacteria was rarely found as a dominant bacterial group, it occupied the highest fraction in a mesopelagic sample in October 2007. Epsilonproteobacteria also showed a similar trend, although its maximal dominance was found in a mesopelagic sample in August 2009. The archaeal community was dominated overwhelmingly by members of the Euryarchaeota in most of the investigations. However, Nitrosopumilales was dominant in aphotic samples in August 2009. Further, their spatiotemporal composition at the family level changed more dynamically in the East Sea. These temporal and spatial distributions of the prokaryotic community were influenced mainly by seawater temperature and depth in the East Sea.  相似文献   

16.
The spatial distributions of δ13C, δ15N, and n-alkanes were investigated to determine the source and transportation of allochthonous organic matter from the mouth of the Seomjin River to the southern inner shelf break of Korea. Total organic carbon (%) ranged from 0.3% to 1.6% (average = 0.80%, n = 81), and the C/N ratio varied from 2.4 to 12.4 (average = 6.76, n = 81). The δ13C values ranged from ?25.86 to ?20.26‰ (average = ?21.47‰, n = 81), and δ15N values ranged from 4.37‰ to 8.57‰ (average = 6.72‰, n = 81). The contribution of the terrestrial fraction of organic matter to the total ranged from 4.4% to 97.7% (average = 24.4%, n = 81), suggesting higher amounts around the catchment area and lower amounts in the offshore area. The concentration of total n-alkanes (nC25 ? nC35) was higher at the boundary between the outer bay and inner shelf break (BOBIS). Average chain length and the carbon preference index both indicated that major leaf wax n-alkanes accounted for the observed distribution of terrestrial organic matter, and were dominant in the inner shelf break (around BOBIS) and outer shelf break. Based on the spatial distribution of the total n-alkanes and the sum of nC27, nC29, and nC31, the terrestrial organic matter distribution was considered to be controlled by local oceanographic conditions, especially at the center of the BOBIS. In addition to enabling the distribution and source of terrestrial organic matter to be identified, the n-alkanes indicated that minor anthropogenic allochthonous organic materials were superimposed on the total organic materials in the central part of Yeosu Bay and the catchment area. The n-alkane indices revealed weathered petroleum contamination, with contamination levels being relatively low at the present time.  相似文献   

17.
The paper presents a new model of electronic-vibrational kinetics of the products of ozone and molecular oxygen photodissociation in the terrestrial middle atmosphere. The model includes 45 excited states of the oxygen molecules O2(b 1, Σ g + ,v= 0−2), O2 (a 1Δ g , v= 0−5), and O2(X 3Σ g − , v= 1−35) and of the metastable atom O (1 D) and over 100 aeronomic reactions. The model takes into account the dependence of quantum yields of the production of O2(a 1Δ g , v= 0−5) in a singlet channel of ozone photolysis in the Hartley band on the wavelength of photolytic emission. Taking account of the electronic-vibrational kinetics is important in retrieval of the vertical profiles of ozone concentration from measured intensities of the Atm and IR Atm emissions of the oxygen bands above 65 km and leads to an increase in the ozone concentration retrieved from the 1.27-μm emission, in contrast to the previous model of pure electronic kinetics. Sensitivity analysis of the new model is made for variations in the concentrations of atmospheric constituents ([O2], [N2], [O(3P)], [O3], [CO2]), the gas temperature, rate constants of the reactions, and quantum yields of the reaction products. A group of reactions that most strongly affect the uncertainty of ozone retrieval from measured intensities of atmospheric emissions of molecular oxygen O2(b 1Σ g + , v) and O2(a 1Δ g , v) has been determined. Original Russian Text ? V.A. Yankovsky, V.A. Kuleshov, R.O. Manuilova, A.O. Semenov, 2007, published in Izvestiya AN. Fizika Atmosfery i Okeana, 2007, Vol. 43, No. 4, pp. 557–569.  相似文献   

18.
The species compositions of the genus Chironomus in the Curonian and Vistula lagoons are very similar, represented by Ch. plumosus and Ch. balatonicus. In the Curonian Lagoon, in addition to these two species, Ch. muratensis and the first-generation interspecific hybrid Ch. muratensis × Ch. plumosus are also found. In this lagoon, Ch. plumosus occurs most frequently (84%); Ch. balatonicus is considered a subdominant species (24%). In the Vistula Lagoon, on the contrary, the high frequency of occurrence is typical of Ch. balatonicus (89%); for Ch. plumosus, it reaches only 11%. The Ch. plumosus population occupies most of the Curonian Lagoon; Ch. balatonicus is present only in its northern part, from the village of Juodkrante to the city of Klaipeda. In the Vistula Lagoon, Ch. balatonicus is distributed over almost its entire area, while Ch. plumosus is found locally, only near the mouth of the Nogat River. The distribution of the sibling species in the lagoons is determined by the salinity gradient.  相似文献   

19.
A series of samples of inhabitants of hydrothermal vents were collected during the 12th cruise of R/V Akademik Mstislav Keldysh in Guaymas Basin (the Gulf of California) and the Axial Seamount area (Juan de Fuca Ridge). The concentrations of trace and heavy metals in the tissues of Ridgeia piscesae, Riftia pachyptila, and Paralvinella palmiformis were analyzed. The neutron-activation analysis revealed significantly higher concentrations of uranium in the tissues of Paralvinella palmiformis as compared to the ambient seawater. The possible reasons for such phenomenon are discussed. The data obtained by the neutron-activation method are compared with those obtained by the atomic-absorption method for the same tissues analyzed.  相似文献   

20.
Since the atmospheric correction is a necessary preprocessing step of remote sensing image before detecting green tide, the introduced error directly affects the detection precision. Therefore, the detection method of green tide is presented from Landsat TM/ETM plus image which needs not the atmospheric correction. In order to achieve an automatic detection of green tide, a linear relationship(y =0.723 x+0.504) between detection threshold y and subtraction x(x=λ_(nir)–λ_(red)) is found from the comparing Landsat TM/ETM plus image with the field surveys.Using this relationship, green tide patches can be detected automatically from Landsat TM/ETM plus image.Considering there is brightness difference between different regions in an image, the image will be divided into a plurality of windows(sub-images) with a same size firstly, and then each window will be detected using an adaptive detection threshold determined according to the discovered linear relationship. It is found that big errors will appear in some windows, such as those covered by clouds seriously. To solve this problem, the moving step k of windows is proposed to be less than the window width n. Using this mechanism, most pixels will be detected[n/k]×[n/k] times except the boundary pixels, then every pixel will be assigned the final class(green tide or sea water) according to majority rule voting strategy. It can be seen from the experiments, the proposed detection method using multi-windows and their adaptive thresholds can detect green tide from Landsat TM/ETM plus image automatically. Meanwhile, it avoids the reliance on the accurate atmospheric correction.  相似文献   

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