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1.
硝酸银络合法浓缩金枪鱼鱼油多不饱和脂肪酸的研究 总被引：1，自引：0，他引：1
以金枪鱼鱼油混合脂肪酸为材料，利用硝酸银络合法浓缩其中的多不饱和脂肪酸(PUFA)，主要考察了搅拌时间和硝酸银浓度对产品得率和EPA和DHA含量的影响，并测定了产品的理化指标和脂肪酸组成。研究结果表明：搅拌时间为60min，硝酸银浓度为4mol／L时得到的产品得率为18．03％，浓缩产品中的EPA和DHA的质量分数分别为9．78％和86．07％，总质量分数达95．85％，过氧化值为7．35mmol／kg，碘值2608．9g／kg。 相似文献
2.
尿素包合法富集蛇鲻鱼油中EPA和DHA的研究 总被引：2，自引：0，他引：2
以蛇鲻精制鱼油为原料,采用氢氧化钾乙醇皂化和硫酸酸化制备鱼油混合脂肪酸,以EPA和DHA含量为指标,探讨了尿素包合法富集鱼油中EPA和DHA的工艺条件。结果表明:以乙醇为溶剂,在尿脂质量比为2︰1和包合温度0℃的条件下进行一次性包合20 h后,富集产物中EPA和DHA总含量达到了69.74%,其中EPA含量为22.05%,DHA含量为47.69%,达到了保健鱼油的生产要求。 相似文献
3.
新鲜鱼油中添加0、50、250、500mg／kg 4种不同浓度的维生素E醋酸酯，在实验的第6、14、28、36和68天测定鱼油中的脂肪酸含量。结果表明：在500mg/kg添加量内，维生素E醋酸酯对鱼油中脂肪酸含量没有显著性影响（P〉0．05）。在实验期间，EPA（C20：5）、DHA（C22：6）、亚油酸（C18：2）含量明显下降，ARA（C20：4）、亚麻酸（C18：3）先升后降，棕榈酸（C16：1）逐步上升；C14含量逐渐增加，C17则先降后升，C16和C18在实验后期有较大提高。维生素E醋酸酯对鱼油中不饱和脂肪酸效用系数大小依次为：ARA〉EPA〉DHA〉C18：3〉0〉C16：1〉C18：2〉C18：1。对ARA抗氧化效用最大，效用系数达到10．193％，C20：5为0．490％，C22：6为0．364％；维生素E醋酸酯对饱和脂肪酸效用系数大小依次为：C14〉C17〉0〉C18〉C16。 相似文献
4.
《广东海洋大学学报》2021,(3)
【目的】探讨不同地域刺参胶原蛋白含量及一般营养成分的差异。【方法】于2019年5月末至7月初在西霞口、旅顺、蚆蛸岛、瓦房店、黑石礁、营城子海域分别选取12只刺参,并测定其干物质中胶原蛋白、粗蛋白、灰分、粗脂肪、总糖及氨基酸和脂肪酸的含量。【结果】养殖刺参(营城子、黑石礁和瓦房店海域)的胶原蛋白、粗蛋白、灰分质量分数显著高于野生刺参(旅顺、蚆蛸岛和西霞口海域)(P0.05);养殖刺参的总脂肪酸TFA、多不饱和脂肪酸PUFAs、DHA和EPA的平均质量分数(4 709.26、2 062.43、94.79 mg/kg)显著高于野生刺参(4 487.03、1 692.31、72.49 mg/kg)(P0.05)。养殖刺参中,营城子刺参(池塘)的单不饱和脂肪酸MUFAs、多不饱和脂肪酸PUFAs及DHA和EPA含量(1 522.87、2 180.94、148.60 mg/kg)显著高于瓦房店刺参(1 366.65、2 001.43、51.37mg/kg)及黑石礁刺参(1 335.37、2 001.92、84.39 mg/kg)(P0.05)。【结论】在一般营养成分组成上,养殖刺参略优于野生刺参,营城子池塘养殖刺参的优于瓦房店池塘养殖刺参及黑石礁室内养殖刺参。 相似文献
5.
新鲜鱼油中添加05、0、2505、00 mg/kg 4种不同浓度的维生素E醋酸酯,在实验的第6、14、28、36和68天测定鱼油中的脂肪酸含量。结果表明:在500 mg/kg添加量内,维生素E醋酸酯对鱼油中脂肪酸含量没有显著性影响(P>0.05)。在实验期间,EPA(C20:5)、DHA(C22:6)、亚油酸(C18:2)含量明显下降,ARA(C20:4)、亚麻酸(C18:3)先升后降,棕榈酸(C16:1)逐步上升;C14含量逐渐增加,C17则先降后升,C16和C18在实验后期有较大提高。维生素E醋酸酯对鱼油中不饱和脂肪酸效用系数大小依次为:ARA>EPA>DHA>C18:3>0>C16:1>C18:2>C18:1,对ARA抗氧化效用最大,效用系数达到10.193%,C20:5为0.490%,C22:6为0.364%;维生素E醋酸酯对饱和脂肪酸效用系数大小依次为:C14>C17>0>C18>C16。 相似文献
6.
测定并分析澳洲长鳍鳗(Anguilla reinhardtii)肌肉中营养成分组成与质量分数。结果显示:澳洲长鳍鳗肌肉中水分质量分数为63.96%,蛋白质质量分数为17.43%,粗脂肪质量分数为16.41%,灰分质量分数为1.74%;肌肉中测出氨基酸17种(未测色氨酸),其中包括人体必需氨基酸7种(占氨基酸总质量的39.97%),必需氨基酸组成基本符合FAO/WHO标准;主要限制性氨基酸为蛋氨酸、胱氨酸和缬氨酸,其必需氨基酸指数(EAAI)为82.61,鲜味氨基酸占氨基酸总质量的45.60%;脂肪酸中多不饱和脂肪酸质量分数为26.73%,其中EPA与DHA的质量分数合计为8.46%。该结果表明,澳洲长鳍鳗有较高的食用价值与保健作用。 相似文献
7.
【目的】分析珍珠龙胆石斑鱼背、腹部肌肉的营养成分与挥发性风味成分。【方法】采用顶空固相微萃取与气相色谱-质谱联用技术检测鱼肉挥发性风味物质,用常规分析法检测鱼肉氨基酸及脂肪酸组成。【结果】珍珠龙胆石斑鱼含肉率达到71.03%±3.88%,鱼鳞与皮质量分数高达9.60%±1.67%。背、腹部肌肉的蛋白质质量分数(干基)分别为85.19%和77.47%,脂肪质量分数分别为6.44%和12.00%,必需氨基酸占总氨基酸质量分数均为0.45;背、腹部肌肉第一限制性氨基酸均为缬氨酸,氨基酸评分(AAS)分别为86和81,氨基酸指数分别为80.06和72.61,均为赖氨酸含量最高,氨基酸评分(AAS)分别为156和140;背部肌肉中5种鲜味氨基酸总量(7.26%)较腹部(7.09%)略高,不饱和脂肪酸分别占肌肉脂肪酸总量的60.11%和45.54%,EPA+DHA的含量是腹部肌肉的11.5倍,质量分数达20.42%,ω-3/ω-6 PUFA为9.56;背、腹部肌肉分别检出挥发性风味物质50与52种,腹部肌肉中醇类物质含量最高,质量分数达60.25%,其不饱和醇类与醛类对其肌肉的总体风味起重要贡献。【结论】珍珠龙胆石斑鱼是一种高蛋白、低脂肪、富含赖氨酸、精氨酸、EPA和DHA的优质海产经济鱼种,其背部肌肉蛋白质营养价值高于腹部,是优质的ω-3不饱和脂肪酸的食物来源;背、腹部肌肉挥发性风味物质含量丰富,腹部肌肉的鲜味优于背部肌肉。 相似文献
8.
对长江下游凤鲚、湖鲚和刀鲚卵巢氨基酸、脂肪酸和矿物元素组成及含量进行了测定分析。结果表明:三种鲚属鱼类卵巢鲜样中总氨基酸、必需氨基酸、呈味氨基酸含量差异不显著;必需氨基酸含量(w)为5.36%～6.32%,占总氨基酸含量为:0.43%、0.41%和0.43%,均以亮氨酸含量最高而色氨酸最低,赖氨酸含量(w)为0.96%～1.04%;饱和脂肪酸、单不饱和脂肪酸和多不饱和脂肪酸差异较显著,脂肪酸组成均以油酸含量最高,EPA和DHA总量(w)为8.00%～11.81%;矿物质含量大多也存在显著差异,常量元素含量最高的为钾(16970～22220mg/kg),微量元素则为锌(262.0～277.0mg/kg);不同水域生长环境对鲚属鱼类卵巢氨基酸含量影响不显著,而对脂肪酸和矿物质含量影响较显著。 相似文献
9.
龙虎斑鱼肌肉营养成分分析 总被引：2，自引：0，他引：2
对龙虎斑鱼的肌肉进行营养成分分析和肉质评价,结果表明,龙虎斑鱼新鲜肌肉中粗脂肪质量分数4.18%,粗蛋白质量分数17.05%,粗灰分质量分数1.24%,水分质量分数80.19%.肌肉蛋白质中共检测出氨基酸种类有17 种,占新鲜肌肉样品的质量分数为17.88%;7 种必需氨基酸（EAA）占新鲜肌肉样品的质量分数为7.79%,占氨基酸总量的43.57%;4 种鲜味氨基酸（DAA）占新鲜肌肉样品的质量分数为6.17%,占氨基酸总量的34.51%;必需氨基酸指数（EAAI）为101.35,其氨基酸组成比例符合FAO/WHO 标准.新鲜肌肉中共检测出脂肪酸种类有14 种.其中7 种饱和脂肪酸（SFA）占总脂肪酸的质量分数为47.29%;4 种单不饱和脂肪酸（MUFA）占总脂肪酸的质量分数为18.86%;3 种多不饱和脂肪酸（PUFA）占总脂肪酸的质量分数为33.86%,DHA 质量分数占脂肪酸总量的30.24%.龙虎斑鱼营养价值较高,海鲜味浓郁. 相似文献
10.
研究了木瓜蛋白酶的酶解温度、酶浓度、酶解时间、加水量、pH值对青鳞鱼可溶性蛋白得率的影响 ,探讨了α 氨态氮含量与青鳞鱼可溶性蛋白得率的关系。结果表明 :温度小于 5 0℃ ,随着酶解温度的提高、酶浓度的增加、酶解时间的延长青鳞鱼可溶性蛋白得率及α 氨态氮的含量均增加 ,α 氨态氮含量增加比可溶性蛋白得率的增加明显 ;加水量的增加 ,可溶性蛋白得率增加 ;pH值对水解效果及可溶性蛋白得率影响不大。综合考虑 ,最佳水解条件为 :温度 5 0℃ ,酶浓度为 1 5 0 0u/g ,酶解时间 80min ,m原料 :m水 =1∶2 ,pH 6 8。酶解所得可溶性蛋白经氨基酸分析仪检测 ,其氨基酸总量为 49 47mg/mL ,必需氨基酸占氨基酸总量的 41 0 % ,游离氨基酸含量为 6 3mg/mL ,肽含量为 87 2 %。 相似文献
11.
对海鲡肌肉的营养成分含量及氨基酸、脂肪酸、无机质组成进行了研究，结果表明，海鲡的蛋白质和脂肪含量分别为21.5%和5.5%，必需氨基酸和鲜味氨基酸分别占氨基酸总量的40.9%和37.8%，不饱和脂肪酸占脂肪酸总量的65.2%，其中C205和C226的含量分别为4.5%和12.0%，3.4kg阶段的养殖海鲡肌肉的必需氨基酸和呈味氨基酸的比率最高。 相似文献
12.
Phaeodactylum tricornutum is a potential livestock for the combined production of eicosapentaenoic acid (EPA) and fucoxanthin. In this study, six marine diatom strains identified as P. tricornutum were cultured and their total lipid, fatty acid composition and major photosynthetic pigments determined. It was found that the cell dry weight concentration and mean growth rate ranged between 0.24–0.36 g/L and 0.31–0.33/d, respectively. Among the strains, SCSIO771 presented the highest total lipid content, followed by SCSIO828, and the prominent fatty acids in all strains were C16:0, C16:1, C18:1, and C20:5 (EPA). Polyunsaturated fatty acids, including C16:2, C18:2, and EPA, comprised a significant proportion of the total fatty acids. EPA was markedly high in all strains, with the highest in SCSIO828 at 25.65% of total fatty acids. Fucoxanthin was the most abundant pigment in all strains, with the highest in SCSIO828 as well, at 5.50 mg/g. The collective results suggested that strain SCSIO828 could be considered a good candidate for the concurrent production of EPA and fucoxanthin. 相似文献
13.
The effects of several nutritional factors on the growth and eicosapentaenoic acid (EPA) production of diatom Nitzschia laevis were studied. 4 LDM (quadrupled concentration of the nutrient salt) was the optimal concentration of nutrient salt for the growth and EPA production ofN. laevis. The growth ofN. laevis was inhibited when the glucose concentration was either lower than 10gL-1 or higher than 15gL-1. Both sodium nitrate and urea were good nitrogen sources for the growth and EPA production, while ammonium chloride seriously decreased the dry cell weight (DW) and the EPA content. Silicate seriously influenced the growth of N. laevis. The maximum DW of 2.34gL-1 was obtained in the presence of 150 mgL-1 Na2SiO3·9H2O. The EPA content remained almost the same when the silicate concentration was lower than 150 mgL-1; however, higher silicate concentrations resulted in a steady de- crease of EPA content. Low medium salinity (≤29) did not seem to influence the DW of N. laevis, and high salinity resulted in a decrease of DW. The highest EPA content (4.08%) and yield (110 mgL-1) were observed at the salinity of 36 and 29, respectively. 相似文献
14.
《中国海洋湖沼学报》2017,(4)
Information of the difference in quality between farmed and wild fish is central to better ensuring fish products produced in aquaculture meet regulatory and consumer requirements.Proximate composition,amino acid and fatty acid profiles,and toxic elements contents of farmed and wild Chanodichthys mongolicus were established and compared.Significantly higher crude protein content while lower moisture content in farmed fish compared to wild fish were observed(P0.05).The percentages of total amino acids(TAA),total essential amino acids(TEAA),total non-essential amino acids(TNEAA)and total delicious amino acids(TDAA) in farmed fish were all significantly higher than those in the wild equivalent(PO.05).Compared to the FAO/WHO recommended reference values,the ratios of TEAA/TAA(39.84%-40.33%) were comparable to 40%and TEAA/TNEAA(66.22%-67.60%) were above 60%.Fatty acid profiles in both fanned and wild C.mongolicus were dominated by monounsaturated fatty acid(MUFA),with farmed fish contained much more MUFA content compared to wild counterpart(P0.05).Notably,wild fish exhibited significantly higher levels of total polyunsaturated fatty acid(PUFA) including eicosapentaenoic acid(EPA) and docosahexaenoic acid(DHA) than farmed fish(P0.05).The EPA(C_(20:5n3))and linoleic acid(C_(18:2n6)) were the predominant PUFA in wild and fanned C.mongolicus,respectively.Moreover,farmed fish displayed an overall lower toxic element levels(As,Cd,Pb and Hg) in comparison with wild fish,and both were far lower than the established limit standard.In conclusion,our results suggest that the nutritional quality of farmed C.mongolicus was inferior to their wild counterpart with respect to fatty acids nutrition,and therefore further studies should focus on the improving C.mongolicus diet in order to enhance the overall nutritional composition. 相似文献
15.
《中国海洋大学学报(英文版)》2016,(2)
The information of trophic relationship is important for studying the Southern Ocean ecosystems.In this study,three dominant krill species,Euphausia superba,Thysanoessa macrura and Euphausia crystallorophias,were collected from Prydz Bay,Antarctica,during austral summer of 2009/2010.The composition of fatty acids in these species was studied.E.superba and T.macrura showed a similar fatty acid composition which was dominated by C14:0,C16:0,EPA(eicosapentenoic acid) and DHA(decosahexenoic acid) while E.crystallorophias showed higher contents of C18:1(n-9),C18:1(n-7),DHA and EPA than the former two.Higher fatty acid ratios of C18:1(n-9)/18:1(n-7),PUFA(polyunsaturated fatty acid)/SFA(saturated fatty acid),and 18PUFA/16 PUFA indicated that E.crystallorophias should be classified as a typical omnivore with a higher trophic position compared with E.superba and T.macrura. 相似文献
16.
金枪鱼油的精炼工艺对脂肪酸组成的影响 总被引：1，自引：0，他引：1
粗鱼油通过精炼可以除去非甘油三酯杂质成分,研究表明黄鳍金枪鱼鱼油精炼的工艺参数为:800 g/L的H3PO4脱胶,添加量为油量的1%(体积分数);300 g/L的NaOH脱酸,添加量为油量的2%(体积分数);活性土脱色,添加量为油量的10%(质量分数);85℃下减压蒸馏脱臭15 min。经过精炼,鱼油呈淡黄色,澄清透明,有淡鱼腥味,理化指标达到鱼油SC/T3502-2000标准的精制鱼油一级要求,精制鱼油中PUFA的总含量(w)达37.06%,DHA和EPA含量(w)分别为25.10%和4.45%。 相似文献
17.
Qiuxing Cai Yanyan Wu Laihao Li Yueqi Wang Xianqing Yang Yongqiang Zhao 《中国海洋大学学报(英文版)》2017,16(5):855-862
Lipid oxidation in salt-dried yellow croaker (Pseudosciaena polyactis) was evaluated during processing with commonly used analytical indices, such as the peroxide value (POV), the thiobarbituric acid reactive substances (TBARS) value, and oxidative-relative lipoxygenase (LOX) activity. Additionally, fatty acids were analyzed using gas chromatography-mass spectrometry. Both POV and TBARS increased significantly (P < 0.05) at the rinsing stage. POV reached its peak value of 3.63 meq O2 per kg sample at the drying stage, whereas TBARS constantly increased from 0.05 to 0.20 mg MDA per kg sample. Processing of salt-dried yellow croaker had an extremely significant (P < 0.01) effect on LOX activity. Twenty-six fatty acids were identified. Combined eicosapentaenoic acid (EPA; C20:5n3) and docosahexaenoic acid (DHA; C22:6n3) content varied between (19.20 ± 0.37) mg g?1 and (23.45 ± 1.05) mg g?1. The polyunsaturated fatty acid/saturated fatty acid (PUFA/SFA) ratio in yellow croaker was 0.73–1.10, and the n-6/n-3 PUFA ratio was approximately 0.13–0.20. The contents of most fatty acids varied significantly (P < 0.05) during the different processing stages, and these differences were caused by lipid oxidation. C18:0, C16:1n7, C19:0, and C22:6n3 showed clear changes in principle component one of a principle components analysis. These fatty acids are potential markers for evaluating lipid oxidation in fish muscle because there was a significant correlation between these markers and TBARS and LOX activity (P < 0.05) with Pearson’s coefficients > 0.931. 相似文献