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1.
在3组一定浓度的副溶血弧菌菌液中分别加入3种浓度的副溶血弧菌噬菌体,第12天各实验组再次分别加入一定浓度的副溶血弧菌或副溶血弧菌噬菌体,比较各组水体中副溶血弧菌噬菌体及其宿主的动态关系。结果表明,副溶血弧菌噬菌体可使宿主下降至一定浓度并与之保持动态平衡关系,实验组1、2、3中副溶血弧菌浓度从2.43×106 mL-1分别下降至6.2×104、9.2×104、7.46×105 mL-1,并以此浓度范围维持下去。高浓度副溶血弧菌噬菌体与其宿主之间平衡关系的稳定性较好,再次加入副溶血弧菌,高、中浓度组分别在1 d和4 d后副溶血弧菌下降至平衡时的数量级浓度,并持续下去。低浓度组副溶血弧菌组有所上升,并持续下去。再次加入副溶血弧菌噬菌体对副溶血弧菌浓度影响较小(P>0.05)。 相似文献
2.
《广东海洋大学学报》2015,(3)
采用复相乳液法,以乙基纤维素为包囊材料,明胶溶液为保护液制取微胶囊,对副溶血弧菌噬菌体(Vibrio parahaemolyticus phage)进行固定化,制备噬菌体微胶囊。结果显示,乙基纤维素质量浓度为30 g/L,明胶质量浓度为20 g/L,搅拌速度为600 r/min,时间30 min,副溶血弧菌噬菌体悬液与乙基纤维素溶液的体积比为1∶3时,所得微胶囊有较好的噬菌体缓释性能,综合评价指数最高。对方斑东风螺(Babylonia areolata)饲以混有噬菌体微胶囊的饵料,当饵料中噬菌体微胶囊质量分数为1.0 g/kg时,与对照组比较,东风螺肠道宿主菌平均减少96%,水体中宿主菌减少72%,差异均有统计学意义(P0.05);以黏附剂按1.5 g/kg的剂量将噬菌体微胶囊黏附于对虾饵料,并投喂凡纳滨对虾(Litopenaeus vannamei),对虾肠道的宿主菌比对照组平均减少86%,水体中宿主菌比对照组减少68%,差异均有统计学意义(P0.05)。微胶囊可有效控制宿主弧菌的在养殖体系的浓度,减少养殖动物弧菌病的产生。 相似文献
3.
研究波吉卵囊藻及藻―菌体系对创伤弧菌(Vibrio vulnificus)、副溶血弧菌(Vibrio parahaemolyticus)和溶藻弧菌(Vibrio alginolyticus)生长的影响,结果显示:无菌波吉卵囊藻对创伤弧菌、副溶血弧菌的生长均有显著促进作用(P<0.05),对溶藻弧菌的生长无显著影响(P>0.05);波吉卵囊藻―沼泽红假单胞菌体系对溶藻弧菌有明显抑制作用(P<0.05);无菌波吉卵囊藻―枯草芽孢杆菌体系对溶藻弧菌的生长有显著促进作用(P<0.05),对创伤弧菌、副溶血弧菌的生长无显著影响(P>0.05);带菌波吉卵囊藻对三株弧菌均有明显抑制作用(P<0.05);带菌波吉卵囊藻共栖细菌对三株弧菌均无明显抑制作用(P>0.05);在无菌波吉卵囊藻中回加带菌藻共栖细菌,分析实验10～18d的数据发现的数据发现藻―菌体系对创伤弧菌、副溶血弧菌的生长有显著影响(P<0.05),藻―菌体系逐渐恢复对三株弧菌的抑制作用。 相似文献
4.
《广东海洋大学学报》2020,(4)
【目的】研究凡纳滨对虾(Litopenaeus vannamei)miRNA-184 [microRNA-184(miR-184),记作LvmiR-184]在健康虾及染病虾中的表达,探讨其免疫功能。【方法】用茎环法克隆鉴定凡纳滨对虾LvmiR-184,通过荧光定量PCR检测LvmiR-184在对虾血淋巴、肝胰腺、鳃、肌肉、心脏、胃、肠和眼柄等8个组织中的分布。对凡纳滨对虾注射副溶血弧菌(Vibrio parahaemolyticus),检测LvmiR-184不同时间点的响应变化。对感染副溶血弧菌的凡纳滨对虾分别注射0.3、3.0和30.0 ng的mi R-184模拟物和抑制物,检测不同时间点对虾血细胞凋亡率。【结果与结论】LvmiR-184在所有组织中均有表达,在胃中表达量最高,其次是肌肉,鳃表达量最低。注射副溶血弧菌后1.5 h,LvmiR-184表达量显著上调;在24 h达到峰值,较对照组高8.25倍;对照组则维持在较低的水平。模拟物、抑制物注射量为30 ng时对虾血细胞凋亡率差异有统计学意义(P 0.05);与对照组凋亡率相比,注射模拟物6 h后提高62%。LvmiR-184可调控凡纳滨对虾的血细胞凋亡。 相似文献
5.
采用K-B法,测定副溶血性弧菌、创伤弧菌和哈氏弧菌等3株方斑东风螺病原弧菌对35种常用抗菌药物的敏感性。结果表明:副溶血性弧菌对大观霉素、复方新诺明、诺氟沙星、头孢吡肟和氧氟沙星敏感;创伤弧菌对复方新诺明、大观霉素、诺氟沙星和多粘菌素B敏感;哈氏弧菌对复方新诺明、大观霉素、环丙沙星、氯霉素、红霉素、哌拉西林、克拉霉素、头孢吡肟、诺氟沙星、左氟沙星、头孢他啶、米诺环素和氧氟沙星敏感。 相似文献
6.
《广东海洋大学学报》2016,(3)
提取溶藻弧菌(Vibrio alginolyticus)的总DNA,克隆溶藻弧菌胱硫醚-β-合成酶基因cbs,对其进行生物信息学分析。结果表明,所克隆溶藻弧菌cbs基因的开放阅读框为1 095 bp,编码364个氨基酸。氨基酸序列比对发现,溶藻弧菌的CBS蛋白与其他弧菌的CBS相似性极高,与副溶血弧菌(V.parahaemolyticus)CBS氨基酸序列相似性达98%;CBS蛋白质的二级结构为典型的"α+β"折叠模式,无跨膜结构及信号肽。构建cbs基因表达载体(p GEX-cbs)以表达重组蛋白,结果显示,cbs基因在大肠杆菌BL21中高效表达,可表达出分子质量约为66.4 ku的融合蛋白。对表达条件进行优化,发现在37℃、IPTG浓度0.6 mmol/L的条件下诱导5 h后,CBS蛋白的表达量较多,该蛋白主要以包涵体形式存在。 相似文献
7.
《广东海洋大学学报》2021,(3)
【目的】研究水解单宁饲养的凡纳滨对虾(Litopenaeus vannamei)在副溶血弧菌(Vibrio parahaemolyticu)胁迫24 h后的生理响应,初步探究水解单宁对凡纳滨对虾血液和血细胞的影响。【方法】在基础饲料中分别添加质量分数为0%(对照组)、0.05%、0.10%、0.15%和0.20%的水解单宁(HTs),饲喂凡纳滨对虾60 d。对对虾注射0.2 mL的副溶血弧菌(2.5×10~8 CFU·mL~(-1)),感染24 h后,分析血细胞凋亡率、活性氧含量(ROS)、一氧化氮含量(NO)、酯酶活性、Toll样受体(TLR)和热休克蛋白70(HSP70)基因的表达水平;测定血清的碱性磷酸酶(AKP)、酸性磷酸酶(ACP)、超氧化物歧化酶(SOD),总抗氧化能力(T-AOC)和溶菌酶(LZM)活性。【结果】在感染副溶血弧菌24 h后,实验组对虾的血细胞凋亡率,ROS、NO水平显著低于对照组,酯酶活性显著高于对照组(P 0.05)。实验组凡纳滨对虾血清的AKP、ACP、SOD活性,T-AOC和LZM活性均显著高于对照组(P 0.05)。实验组对虾血细胞TLR和HSP70基因的表达水平显著上调(P 0.05)。【结论】饲料中添加水解单宁可提高凡纳滨对虾的非特异性免疫能力和抗氧化功能,阻止氧化损伤的发生,清除机体受到刺激产生的ROS和NO,增强酯酶活性,减少细胞凋亡,从而提高凡纳滨对虾抗副溶血弧菌感染的能力。 相似文献
8.
【目的】研究广东湛江地区凡纳滨对虾(Litopenaeus vannamei)烂尾病主要病原及其药敏特征。【方法】从对虾病灶部位分离纯化细菌,用2株代表菌株对健康虾进行创伤浸浴感染试验,分析菌株形态特征、生理生化特征以及16S rRNA和HSP60基因序列,以哈维氏弧菌(Vibrio harveyi)和坎氏弧菌(V. campbelli)溶血素基因特异引物扩增菌株基因组DNA,通过纸片扩散法测试菌株对17种药物的敏感性。【结果】从患病亲虾和养殖对虾分别分离获得编号2018B22和2018MZ1的代表菌,两株菌16Sr RNA和HSP60基因序列均与哈维氏弧菌最相似,且均可被哈维氏弧菌溶血素基因引物特异扩增,表型特征亦与哈维氏弧菌相近;两株菌均可引起凡纳滨对虾烂尾病,其中菌株2018B22的致病力较2018MZ1更强,而后者的耐药谱更广。【结论】湛江地区凡纳滨对虾烂尾病主要病原为哈维氏弧菌。 相似文献
9.
从马氏珠母贝(Pinctada martensii)插核部位蘸取组织液分离弧菌,研究插核手术前后室内休养3 d时伤口部位弧菌类型的变化,比较使用不同小片和珠核处理液对手术伤口部位弧菌类型的影响。形态和生理生化特征分型和鉴定结果显示,插核前和休养期贝样上分离的优势弧菌类型无明显差异,只是休养期弧菌的分离频率有所下降;从术后不同处理组的马氏珠母贝伤口上均分离得到7个类型弧菌,弧菌所属的种类也一致,而从未插核的对照组相应部位上只分离到4个类型弧菌,除未分离到副溶血弧菌(Vibrio parahaemolyticus)外,对照组与两个处理组贝样上分离到的弧菌种类基本上一致。 相似文献
10.
采用溶藻弧菌ATCC17749菌体作为抗原免疫新西兰大白兔,制备兔抗溶藻弧菌多克隆抗体;利用柠檬酸钠还原氯金酸法制备胶体金及胶体金-抗体结合物,并将其喷涂在玻璃纤维膜上冷冻干燥;在硝酸纤维素膜上包被兔抗溶藻弧菌多克隆抗体和羊抗兔IgG分别作为检测线和控制线;将处理后的玻璃纤维素膜、硝酸纤维膜、样品垫及吸水纸组装成双抗夹心试纸条,并对试纸条灵敏度、特异性、稳定性进行评价。结果表明,制备的兔抗溶藻弧菌多克隆抗体的效价可达1∶512 000;胶体金溶胶的最佳制备条件为:加入1.8 mL 10 mg/mL的柠檬酸三钠,500 W微波炉中火加热10 min;试纸条检测线和质控线的抗体最佳包被量分别为8.0 mg/mL和1.0 mg/mL;试纸条检测灵敏度为1×106cfu/mL,特异性试验显示,试纸条与哈氏弧菌、副溶血弧菌、霍乱弧菌、拟态弧菌、美人鱼弧菌、弗氏弧菌、河流弧菌、创伤弧菌等8株菌无交叉反应;稳定性试验表明,试纸在4℃干燥条件下能稳定保存5个月以上。制备的溶藻弧菌多克隆检测试纸条可灵敏、较特异地检测源自病鱼的溶藻弧菌,整个检测过程可在10 min内完成,适用于溶藻弧菌的快速检测。 相似文献
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1 IntroductionCloudybandsaretypicalstratigraphicstructureindeepicecoresandtheylookwhitecolorwhenaflorescentlightwasirradiated.InWisconsinice ageiceattheGreenland ,manycloudybandsarelocatedincoldperiodsiceandcloudybandsareusedfordatingofdeepicecoresincepairofseriescloudybandandclearlayercorrespondstoannuallayer(Alleyetal.1 997;Meeseetal.1 997) .AndcloudybandcorrespondstovolcaniclayerfortheAntarcticicecore (GowandWilliamson 1 976) .Therefore,identificationofcloudybandsisimportantworkfordatin… 相似文献
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13.
Response of internal waves to 2005 Typhoon Damrey over the northwestern shelf of the South China Sea
Gang Wang Fangli Qiao Yijun Hou Dejun Dai Min Lin Qilong Zhang Gang Wang Baoshu Yin 《中国海洋大学学报(英文版)》2008,7(3):251-257
Continuous observation of sea water temperature and current was made at Wenchang Station (19°35′N, 112°E) in 2005. The data collected indicate vigorous internal waves of both short periods and tidal and near-inertial periods. The temperature and current time series during 18-30 September were examined to describe the upper ocean internal wave field response to Typhoon Damrey (0518). The strong wind associated with the typhoon, which passed over the sea area about 45 km south of Wenchang Sta- tion on 25 September, deepened the mixed layer depth remarkably. It decreased the mixed layer temperature while increasing the deep layer temperature, and intensified the near-inertial and high-frequency fluctuations of temperature and current. Power spectra of temperature and current time series indicate significant deviations from those obtained by using the deep ocean internal wave models characterized by a power law. The frequency spectra were dominated by three energetic bands: around the inertial frequency (7.75× 10-6 Hz), tidal frequencies (1.010-25 to 2.4×10-5 Hz), and between 1.4×10-4 and 8.3 × 10-4 Hz. Dividing the field data into three phases (before, during and after the typhoon), we found that the typhoon enhanced the kinetic energy in nearly all the frequency bands, es- pecially in the surface water. The passage of Damrey made a major contribution to the horizontal kinetic energy of the total surface current variances. The vertical energy density distribution, with its peak value at the surface, was an indication that the energy in- jected by the strong wind into the surface current could penetrate downward to the thermocline. 相似文献
14.
光温敏核不育水稻幼穗育性转换期蛋白质组的初步分析 总被引：2，自引：0，他引：2
为找寻光温敏核不育水稻幼穗与育性调控有关的蛋白质,采用固相pH梯度-SDS聚丙烯酰胺双向凝胶电泳对光温敏核不育水稻(培矮64S)育性转换期幼穗总蛋白进行了分离,获取凝胶图像并进行重复性分析,通过微量测序-Edman降解方法和原位酶解及MALDI-TOF质谱的方法鉴定了60个蛋白质点。结果表明:本方法可得取分辨率和重复性较好的凝胶图谱。蛋白质点在2D胶上的重复性为:沿等电聚焦方向偏差为1.49±0.22 mm,沿SDS-PAGE方向偏差为1.24±0.18 mm。37个蛋白质点得到了准确的鉴定结果,其中16个是差异蛋白质点。在不育变化为可育的过程中,明显表达上调的蛋白质点包括几丁质酶,丙酮酸脱氢酶等;明显下调的蛋白质包括超氧化物歧化酶,硝酸还原酶脱辅基酶蛋白等;金属硫蛋白RicMT只在可育中存在。 相似文献
15.
建鲤与黄河鲤的RAPD分子标记及其杂交优势的遗传分析 总被引：3，自引：0，他引：3
利用RAPD技术对建鲤、黄河鲤及其杂交子代进行了遗传分析,筛选的33个引物共扩增出155条带,其中多态性片段为105条,片段S18-1600、S472-300具有种的特异性,可作为鉴别建鲤与黄河鲤的分子遗传标记。建鲤、黄河鲤及其正、反交子代群体内的遗传相似系数分别为0.8240、0.7921、0.7920、0.8569,黄河鲤与正交F1具有较高的遗传变异水平,反交F1变异最小。正交F1、反交F1与亲本(建鲤、黄河鲤)的遗传距离分别为0.2233、0.2436、0.1749、0.2026,说明子代均继承了较多的建鲤的遗传物质。分析了子代与亲本的RAPD标记类型以及各类型的条带数。应用PIT标记系统测定了子代的杂种优势并探讨了优势产生的遗传机理。 相似文献
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Behavior of different phosphorus species in suspended particulate matter in the Changjiang estuary 总被引：1，自引：0，他引：1
Suspended particulate matter (SPM) collected in the Changjiang (Yangtze River) estuary in June 2006 was separated into five
fractions via water elutriation: clay-very fine silt (<8 μm), fine silt (8–16 μm), medium silt (16–32 μm), coarse silt (32-63
μm) and sand (>63 μm). The SPM and fractionated particles were sequentially analyzed by a modified SEDEX sequential extraction
method to obtain six species of phosphorus: exchangeable or loosely-sorbed P, organic P, Fe-bound P, authigenic P, detrital
P and refractory P. The results indicated that all particulate phosphorus species except for detrital P were negatively correlated
to particle size; a high detrital P content was found in coarse silt and very coarse silt. From the inside of the river mouth
to the gate of the river mouth, organic P, Fe-bound P and refractory P in the suspended particles decreased and a higher amount
of exchangeable P appeared around the gate of the river mouth. From the gate of the river mouth to the sea, exchangeable P
and organic P in suspended particles increased distinctly. The total particulate P flux into the estuary from the Changjiang
River was about 45.45×108 μmol/s during sampling. Of this, about 8.27×108 μmol/s was associated with the “truly suspended” fraction. The bio-available particulate P flux was about 13.58×108 μmol/s. Of this, about 4.24×108 μmol/s was transported by “truly suspended” particles. 相似文献
17.
An oligopeptide permease A(OppA)was purified from the extracellular product of Vibrio harveyi SF-1.The molecular weight of the purified protein was estimated to be 58 kDa on SDS-PAGE.The purified protein showed phospholipase C activity at the optimal values of temperature 50℃ and pH 8.0.The enzymatic activity decreased when the temperature increased to 40℃.The N-terminal sequence of the purified protein was determined as ADVPAGTKLA,which is similar to that of OppA.The OppA pre-cursor gene was cloned from th... 相似文献
18.
自动子空间划分在高光谱影像波段选择中的应用 总被引：2，自引：0，他引：2
针对高光谱遥感影像数据量大、维数高的特点,结合联合熵波段选择算法,提出了一种自动子空间划分的改进方案。该方法充分利用了影像各波段数据之间的局部相关性,根据波段间相关系数矩阵图像的"分块"特点,将整个波段空间自动划分为若干个子空间,然后再进行波段选择。实现了在删减冗余信息的同时选择出含有主要信息的特征波段组合的目的。将此方法得到的结果与用联合熵得到的结果进行了比较分析,结果表明自动子空间划分的联合熵波段选择方法具有较好的效果。 相似文献
19.
Huaming Yu Lu Wang Liang Kuang Haiqing Yu Yuchen Sun Yuhuan Qu Xin Wu 《中国海洋大学学报(英文版)》2016,15(2):209-218
Based on the finite-volume coastal ocean model(FVCOM),a three-dimensional numerical model FVCOM was built to simulate the ocean dynamics in pre-dam and post-dam conditions in Bachimen(BCM).The domain decomposition method,which is effective in describing the conservation of volume and non-conservation of mechanical energy in the utilization of tidal energy,was employed to estimate the theoretical tidal energy resources and developable energy resources,and to analyze the hydrodynamic effect of the tidal power station.This innovative approach has the advantage of linking physical oceanography with engineering problems.The results indicate that the theoretical annual tidal energy resources is about 2×10~8 k Wh under the influence of tidal power station;Optimized power installation is confirmed according to power generation curve from numerical analysis;the developable resources is about 38.2% of theoretical tidal energy resources with the employment of one-way electricity generation.The electricity generation time and power are 3479 hours and 2.55×10~4 KW,respectively.The power station has no effect on the tide pattern which is semi-diurnal tide in both two conditions,but the amplitudes of main constituents apparently decrease in the area near the dam,with the M_2 decreasing the most,about 62.92 cm.The tidal prism shrinks to 2.28×10~7 m~3,but can still meet the flow requirement for tidal power generation.The existence of station increases the flow rate along the waterway and enhances the residual current.There are two opposite vortexes formed on the east side beside the dam of the station,which leads to pollutants gathering. 相似文献
20.
It is of significance to research failure mechanism of debris landslides that are widespread in the Three Gorges Reservoir Area. Based on the statistical analysis of the developmental law and failure mode of debris landslides in the Three Gorges Reservoir, the mode of progressive failure is found. The mechanical model for progressive failure of debris landslides with two slip bands is also established by applying slice method. According to the results of the downslide force between adjacent slices, if the downslide force of lower slice is larger than zero, the slice fails along the major sliding surface, otherwise it is stable. In result, the failure range is obtained. The stress function can be determined through dimensional analysis of failure slice. According to static boundary conditions of the slice, stress state of any point in the slice can be obtained. Then stress state of any point in the secondary slip band can also be established. The failure of the secondary slip band is judged on the basis of Mohr-Coulomb failure criterion. Therefore, a mechanical method is proposed to analyze the progressive failure of debris landslide with two slip bands. 相似文献