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1.
The aim of this study was to quantify the N removal efficiency of an Ulva-based phytotreatment system receiving wastewaters from a land-based fish farm (Orbetello, Italy), to identify the main biogeochemical pathways involved and to provide basic guidelines for treatment implementation and management. Fluxes of O2 and nutrients in bare and in Ulva colonised sediments were assessed by light/dark core incubations; denitrification by the isotope pairing technique and Ulva growth by in situ incubation of macroalgal disks in cages. O2 and nutrient budgets were estimated as sum of individual processes and further verified by 24-h investigations of overall inlet and outlet loads. Ulva uptake (up to 7.8 mmol Nm(-2) h(-1)) represented a net sink for water column and regenerated NH4+ whilst N removal via denitrification (10-170 micromol Nm(-2) h(-1)) accounted for a small percentage of inorganic nitrogen load (<5%). Laboratory experiments demonstrated a high potential for denitrification (over 800 microM Nm(-2) h(-1)) indicating that N loss could be enhanced. The control of Ulva standing stocks by optimised harvesting of surplus biomass may represent an effective strategy to maximise DIN removal and could result in the assimilation of approximately 50% of produced inorganic nitrogen.  相似文献   

2.
We examined the relevance of dissolved inorganic nitrogen (DIN) forms (nitrate and ammonium) in stream water as N sources for different macrophyte species. To do this, we investigated the variability and relationships between 15N natural abundance of DIN forms and of four different macrophyte species in five different streams influenced by inputs from wastewater treatment plants and over time within one of these streams. Results showed that 15N signatures were similar in species of submersed and amphibious macrophytes and in stream water DIN, whereas 15N signatures of the riparian species were not. 15N signatures of macrophytes were generally closer to 15N signatures of nitrate, regardless of the species considered. Our results showed significant relationships between 15N signatures of DIN and those of submersed Callitriche stagnalis and amphibious Veronica beccabunga and Apium nodiflorum, suggesting stream water DIN as a relevant N source for these two functional groups. Moreover, results from a mixing model suggested that stream water DIN taken up by the submersed and amphibious species was mostly in the form of nitrate. Together, these results suggest different contribution to in-stream N uptake among the spatially-segregated species of macrophytes. While submersed and amphibious species can contribute to in-stream N uptake by assimilation of DIN, macrophyte species located at stream channel edges do not seem to rely on stream water DIN as an N source. Ultimately, these results add a functional dimension to the current use of macrophytes for the restoration of stream channel morphology, indicating that they can also contribute to reduce excess DIN in streams.  相似文献   

3.
湖北省三道河水库底栖动物的初步研究   总被引:4,自引:2,他引:2  
2007年8月至2008年4月,在湖北省三道河水库6个采样点调查底栖动物,共采集到底栖动物17种,其中水生昆虫11种,均为摇蚊科;寡毛类6种,均为颤蚓科,春季优势种为前突摇蚊,夏季优势种为小摇蚊、流水长跗摇蚊和前突摇蚊,秋、冬季优势种为霍甫水丝蚓,底柄动物种类数、密度和生物量随季节变化明显,种类数夏季>冬季>秋季>春季,密度和生物量春季>冬季>秋季>夏季,周年密度和生物量分别为366.42±102.93ind/m2和0.5649±0.1779g/m2,属贫营养型水库,现存量自坝前向库尾逐步递增,Shannon-Wiener指数、Margalef丰富度指数和Pielou均匀度指数均在夏季最高,秋季最低,4次调查中寡毛类密度均未超过1 000ind./m2,水质属轻度污染.  相似文献   

4.
We estimated phosphate uptake in an eutrophic and macrophyte-rich Pampean stream, in a basin where dominant land use was agricultural activity. Our hypothesis was that phosphate retention may be linked to macrophyte abundance, especially that of submerged plants. Four short-term constant-rate phosphate addition experiments were carried out in late spring, summer, autumn and winter under different discharge, background phosphate concentration and macrophyte abundance. Changes in added soluble reactive phosphorus (SRP) concentration were monitored along the studied reach and corrected for dilution to estimate phosphate uptake length (Sw) and other spiraling metrics (Vf or mass transfer coefficient, and U or nutrient uptake rate). All spiraling metrics indicated a higher SRP retention in spring than in autumn, despite macrophyte cover was similar in both sampling dates (39% and 51%, respectively). Phosphate retention was not detected neither in summer (when macrophyte biomass and especially that of submerged plants was the highest one) nor in winter. Ambient SRP concentrations in the stream were an order of magnitude higher than concentrations observed in pristine streams; nevertheless, under the varying conditions of discharge and phosphate levels observed in our study, we found no retention in summer and winter, a phosphate uptake comparable with those measured in impaired streams in autumn, and with those measured in pristine streams in late spring.  相似文献   

5.
太湖贡湖湾虾类种类组成与时空分布特征   总被引:3,自引:1,他引:2  
温周瑞  谢平  徐军 《湖泊科学》2011,23(6):961-966
分别于2005年4月、7月、10月利用蹦网对贡湖湾虾类种类组成和时空分布进行调查.共采集到虾类9605尾,属于2科3属5种,分别是秀丽白虾(Exopalaemon modestus)、日本沼虾(Macrobrachium nipponense)、锯齿新米虾中华亚种(Neocaridina denticulata sin...  相似文献   

6.
武山湖是紧邻长江的通江型富营养化湖泊,是国家级湿地公园.为切实了解该湖在以鲢、鳙养殖为主的情况下浮游植物结构特征,于2017年9月-2018年8月对其浮游植物群落结构特征及水质开展了监测.监测结果表明武山湖水质全年处于轻度富营养到重度富营养水平之间;12次共采集浮游植物7门100种(属),浮游植物优势种共有23种,其中蓝藻门有9种,绿藻门有8种,硅藻门和隐藻门各有3种.夏季和秋季蓝藻门优势种最多且优势度高,冬季和春季绿藻门和硅藻门优势种多且优势度高.武山湖浮游植物每月优势度最大的种类主要有蓝藻门微囊藻和细小平裂藻、绿藻门小球藻以及硅藻门小环藻.浮游植物生物量峰值出现在6月,达34.77 mg/L;丰度峰值出现在7月,达341.46×106 cells/L.冗余分析(RDA)和线性相关分析均表明浮游植物丰度和生物量与总磷、温度和pH均呈显著正相关,且蓝藻门生物量和丰度以及优势属与总磷和温度均呈显著正相关.研究结果表明武山湖浮游植物丰度和生物量在夏季均很高,发生蓝藻水华的风险较大.相对于氮,磷是更重要的限制浮游植物生长的营养元素.  相似文献   

7.
We examined nutrient flux, uptake, and transformation along a spring-fed stream in the Ozark region of Missouri, USA, over the year 2006. Water in Mill Creek originates from several springs, with a single spring contributing over 90% of the stream discharge during much of the year of study. Soluble reactive phosphate concentrations were usually low (<10 μg L−1) along Mill Creek, but peaked during high discharge. Concentrations of dissolved inorganic nitrogen (DIN) were relatively high in the spring water, mainly as nitrate, but usually declined across a small pond and the 10-km length of Mill Creek. During low flows in summer and early autumn, the stream removed over 300 μg L−1 of DIN over its 10-km length, or about 80% of the initial amount. DIN retention along the stream, as a percentage of the DIN upstream, was related mainly to discharge, with higher flows having much higher DIN concentrations. The net uptake rate of DIN uptake was 0.91 μg m−2 s−1 in the stream during summer baseflow. The uptake rate declined downstream for different reaches and was closely related to DIN concentration. In experimental channels, uptake by epilithic algae was one significant sink for nitrate-N in Mill Creek. In 2006, inorganic nutrient export during a single day after a spring storm was similar to export during 40–100 days of low flow conditions in summer and early autumn. Our results suggest that significant nutrient retention can occur during baseflow periods via biological uptake, whereas substantial export occurs during high flow conditions.  相似文献   

8.
Chemical compositions of Coscinodiscus wailesii were determined for four samples of natural cells. Results revealed that the cellular Si:N ratio of C. wailesii cell was 2.4:1.0-5.2:1.0. The impacts of C. wailesii on surrounding coastal water were evaluated from hydrographic observations, in which C. wailesii cell density, nutrients concentrations and temperature were monitored from November 2001 to February 2005 in Harima-Nada, the Seto Inland Sea, Japan. In low temperature periods, from October to December, two peaks of C. wailesii cell density were observed and nutrient concentrations were depleted. The draw-down ratio of Si(OH)(4) and DIN (Si(OH)(4):DIN ratio) in the water column were similar to the cellular Si:N ratio of C. wailesii cells, which have high Si contents. In addition the effects of different Si(OH)(4):DIN ratio were determined for in situ bottle incubation experiments. In the culture experiments, picoplankton (0.2-2.0 microm) which consisted of small flagellates became dominant under low Si(OH)(4):DIN ratios. These results suggested that the sizes distribution shifted to small size and the phytoplankton community was changed to small flagellates after the C. wailesii bloom. These changes would influence predators at higher trophic levels in its coastal ecosystem.  相似文献   

9.
This study presents input–output budgets of total dissolved nitrogen (TDN), dissolved organic N (DON) and dissolved inorganic N (DIN) for a reservoir in a peatland catchment in the south Pennines (UK). This site receives high levels of atmospheric inorganic N deposition, in the range of 26 kg N ha?1 yr?1. The results show that the reservoir retains ~21 to 31% of the annual TDN input (8806 ± 741 kg N). Approximately 39 to 55% of DON (3782 ± 653 kg N) and 6 to 13% of DIN (5024 ± 349 kg N) were retained/processed. A long water retention time (104 days), average annual pH of 6.5, high concentrations of DIN in the reservoir water and a deep water column suggest that denitrification is potentially a key mechanism of N retention/removal. The results also demonstrate that DON is potentially photodegraded and utilized within the reservoir, particularly during the summer season when 58 to 80% of DON input (682 ± 241 kg N) was retained, and a net export of DIN (~34 kg N) was observed. The findings therefore suggest that DON may play a more crucial role in the biogeochemistry of peat‐dominated acid sensitive upland freshwater systems than previously thought. Reservoirs, impoundments and large lakes in peatland catchments may be important sites in mediating downstream N transport and speciation. Copyright © 2016 John Wiley & Sons, Ltd.  相似文献   

10.
为了解南水北调中线干渠浮游植物群落的时空格局,自2018年8月开始分4个季度对干渠沿程的11个样点进行采样调查,同步开展水环境调查;共检出浮游植物7门83属145种,物种丰富度上,硅藻种类数最多,绿藻和蓝藻分别占第2和第3位,且春季>秋季>冬季>夏季;优势种包括极小曲丝藻(Achnanthidum minutissimum)、桥弯藻(Cymbella sp.)、空星藻(Coelastrum sphaericum)、衣藻(Chlamydomonas sp.)等(优势度Y>0.02);全年叶绿素a浓度为(4.09±2.89)μg/L,细胞密度为(6.79±7.15)×106cells/L,夏季细胞密度显著高于其他季节,以绿藻、蓝藻为主,其他季节以硅藻、绿藻为主;空间上,细胞密度从南到北呈递增趋势,穿黄工程后显著高于穿黄工程前;RDA与多元线性回归分析显示:水温和营养盐是影响群落结构的关键因素,其次是流量和流速,这些因子成为群落时空格局的决定因子;NMDS与聚类分析显示:时间变化对于群落结构产生的影响比空间变化更为显著.本研究确认了跨流域长距离输水的中线干渠中浮游植物群落的显著时空异质性;鉴于流速、流量对群落的决定作用,建议在输水管理中加强生态调度技术研究,以调控浮游植物群落,保障水质稳定.  相似文献   

11.
我国淡水湖库频发水华,不同类群形成的水华特征、危害及其治理方法差异显著,因此,如何区分不同藻种的遥感反射率特征,获取湖泊优势种信息是一个亟待解决的科学问题。研究基于室内藻种培养实验,培养了富营养化湖泊中的典型蓝藻和绿藻藻种,其中,蓝藻包括铜绿微囊藻(Microcystis aeruginosa)、假鱼腥藻(Pseudanabaenasp.)和束丝藻(Aphanizomenonsp.),绿藻包括小球藻(Chlorellasp.)以及四尾栅藻(Scenedesmus quadricauda);基于实测的遥感反射率,经归一化处理后,分析了不同藻种的遥感反射率特征,构建了DI(difference index)指数以及ADI(algae distinguish index)指数,建立了藻种分类模型,利用验证集数据进行检验,整体识别精度达77.55%,Kappa系数为0.7178。将分类方法应用于太湖野外实测遥感反射率数据集中,结果与实测的生物量数据有较好的匹配;将模型应用于太湖OLCI(ocean and land colour instrument)影像数据,获得了2019年12月和2020...  相似文献   

12.
The objectives of this study were to measure: (1) Irgarol and GS26575 (major metabolite) during the peak 2004 boating season at selected marinas and reference areas in the Carolinian Zoogeographic Province of the Eastern United States; (2) Irgarol and GS26575 at selected stations during the summer months in the Back Creek/Severn River area in Maryland in 2003 and 2004; and (3) structural and functional characteristics of resident phytoplankton communities concurrently with Irgarol and GS26575 monitoring in Back Creek/Severn River area. Irgarol concentrations from 14 marinas in the Carolinian Province ranged from non-detectable (<1 ng/L) to 85 ng/L; concentrations were less than 16 ng/L at all reference sites. The probability of exceeding the plant 10th centile for Irgarol (251 ng/L) was less than 0.6% for all marinas and 0.01% for all reference areas. These data suggest low ecological risk from Irgarol exposure for both marina and reference areas in the Carolinian Province. Irgarol concentrations ranged from 5 ng/L at the Severn River reference site to 1,816 ng/L in Port Annapolis marina during the two year study. Ecological risk from Irgarol exposure was high for the Port Annapolis marina sites based on a probability of exceeding the plant 10th centile. However, risk was low for Severn River and Severn River reference sites. Functional and structural measures of resident phytoplankton communities in the Back Creek and Severn River did not suggest that these target species are impaired in the Port Annapolis marina area where probabilistic analysis predicted adverse effects from Irgarol exposure.  相似文献   

13.
Toxicity Identification Evaluations (TIEs) can be used to determine the specific toxicant(s), including ammonia, causing toxicity observed in marine sediments. Two primary TIE manipulations are available for characterizing and identifying ammonia in marine sediments: Ulva lactuca addition and zeolite addition. In this study, we compared the efficacy of these methods to (1) remove NH(x) and NH(3) from overlying and interstitial waters and (2) reduce toxicity to the amphipod Ampelisca abdita and mysid Americamysis bahia using both spiked and environmentally contaminated sediments. The utility of aeration for removing NH(x) and NH(3) during a marine sediment TIE was also evaluated preliminarily. In general, the U. lactuca and zeolite addition methods performed similarly well at removing spiked NH(x) and NH(3) from overlying and interstitial waters compared to an unmanipulated sediment. Toxicity to the amphipod was reduced approximately the same by both methods. However, toxicity to the mysid was most effectively reduced by the U. lactuca addition indicating this method functions best with epibenthic species exposed to ammonia in the water column. Aeration removed NH(x) and NH(3) from seawater when the pH was adjusted to 10; however, very little ammonia was removed at ambient pHs ( approximately 8.0). This comparison demonstrates both U. lactuca and zeolite addition methods are effective TIE tools for reducing the concentrations and toxicity of ammonia in whole sediment toxicity tests.  相似文献   

14.
抚仙湖浮游甲壳动物群落结构与空间分布   总被引:4,自引:3,他引:1  
云南抚仙湖是我国典型的高原深水湖泊,为揭示湖中浮游甲壳动物对生态系统变化的响应,研究了其群落结构与空间分布特征.2005年6-7月在抚仙湖共采集浮游甲壳动物8种,平均密度为27.6ind./L,优势类群为桡足类.桡足类优势种为舌状叶镖水蚤(Phyllodiaptomus tunguidus Shen et Tai),枝角类优势种为角突网纹涵(Ceriodaphnia cornuta Sars)和象鼻溞(Bosmina sp.).浮游甲壳动物主要分布在0-30m水层,密度随水深增加而下降;0-10m水层,枝角类和无节幼体占优势,大于10m水层,桡足类占优势.浮游甲壳动物密度南区大于北区(P<0.01),而沿岸区与湖心区之间、沉水植物区与非沉水植物区之间无显著性差异(P>0.05).相关分析表明:浮游甲壳动物密度的垂直分布与藻类密度和水体理化凼子相关关系显著,而水平分布与藻类密度和水体理化因子相关关系不显著.与20世纪80年代相比,抚仙湖浮游甲壳动物优势种发生了演替,西南荡镖水蚤(Neutrodiaptomus mariadviagae mariadviagae (Brohm))消失,被舌状叶镖水蚤代替,可能与太湖新银鱼(Neosalanx taihuensi Chen)引入有关;浮游甲壳动物密度表现出增加趋势,但仍处于较低水平,与水体营养水平的提高及太湖新银鱼的捕食压力有关.  相似文献   

15.
Hong Kong is surrounded by estuarine, coastal and oceanic waters. In this study, monthly averages over a 10 year time series of salinity, temperature, chlorophyll a (chl a), dissolved oxygen (DO), dissolved inorganic nitrogen (DIN), silicate (SiO4) and orthophosphate (PO4) at three representative stations around Hong Kong were used to examine if excess nitrogen in estuarine influenced waters is due to P limitation. The monthly distribution clearly shows the dominant influence of the seasonal change in river discharge in the Pearl River estuary and adjacent coastal waters. In winter, the river discharge is small and more oceanic waters are dominant and as a result, salinity is high, and chlorophyll and nutrients are low. In summer, when the river discharge is high, salinity decreases and nutrients increase. DIN is very high, reaching 100 μM in the estuary. This indicates over enrichment of nitrogen relative to P and consequently there is an excess of N in coastal waters of Hong Kong. P remains low (∼1 μM) and can potentially limit both phytoplankton biomass and N utilization which was demonstrated in field incubation experiments. P limitation would result in excess N being left in the estuarine influenced waters south of Hong Kong. Phosphate concentration is lower in the Pearl River estuary than in many other eutrophied estuaries. Therefore, this relatively low PO4 concentration should be a significant factor limiting a further increase in the magnitude of algal biomass and in the degree of eutrophication in the Pearl River estuary. The export of the excess N offshore into the northern South China Sea may result in an increase in the size of the region that is P limited in summer.  相似文献   

16.
《Marine pollution bulletin》2012,65(12):2699-2707
In 2007, the Rhodophyceae Alsidium corallinum C. Ag., a marine taxon, bloomed in the eutrophic lagoon of Orbetello (Tuscany, Italy) for the first time, becoming the dominant species in spring and summer. In November, its biomass collapsed. The hypothesis examined in this study is that the bloom expressed a relatively low eutrophic level of the ecosystem after intense disposal of accumulated sedimentary organic matter (OM) by dystrophic processes in the two years preceding the bloom. To verify the hypothesis, we compared water physical–chemical variables, sediment redox (Eh) and OM, and standing crops of macroalgae and seagrass from the database of routine monitoring between 2005 and 2008. We also used dissolved nutrient data obtained in 2007 and 2008, as well as data on chlorophyll and total suspended matter in the water column during the microalgal bloom of 2007, and C, N and P content in thalli of the Chlorophycea Chaetomorpha linum and the Rhodophyceae Gracilariopsis longissima and A. corallinum obtained in 2007.In 2007, unusually low values of dissolved inorganic nitrogen (DIN) were recorded. Combined with stable values of soluble reactive phosphorus (SRPs), low DIN led to a reduction of about one order of magnitude in the DIN:SRP atomic ratio with respect to the past and to 2008. G. longissima accumulated C, N and P more than the other species and A. corallinum proved to be less demanding. Sediment OM was lower in the autumn of years characterized by dystrophy, confirming that summer dystrophic events coincided with maximum energy dissipation in this ecosystem. However, as soon as OM and DIN values increased (2008), the vegetation shifted towards blooms of G. longissima and C. linum, while A. corallinum almost disappeared. The results sustain the hypothesis that the bloom of A. corallinum was due to a decline in DIN that limited G. longissima, and to intense turbidity of the water caused by microphytes that developed after the dystrophic event of summer 2006. The latter probably limited the development of C. linum, which could only develop at the edges of the lagoon.  相似文献   

17.
Based on data collected at 31 stations and 1 continuous station in the Pearl River estuary during cruises of July 1999 (rainy season) and January 2001 (dry season), this study examined taxonomic composition, abundance, and spatial distribution of phytoplankton. Results indicated 130 species of phytoplankton in the samples from the rainy season, and 132 species in the dry season. Among them, in the rainy season, 82 species of diatom, 39 fresh-water and half-fresh-water species and 41 species of red tide organisms were found. Within these, there were 54 tropical and sub-tropical species, 47 cosmopolitan species and 17 temperate species. The abundance of phytoplankton in the rainy season was higher than that of the dry season, with an average of 6.3 x 10(5) cells x L(-1) and 1.4 x 10(5) cells x L(-1), respectively. Diversity index (H') and evenness (J) were 2.47 and 0.57 in the rainy season, and 2.01 and 0.54 in the dry season. The dominant phytoplankton species in the rainy season was Skeletonema costatum with an average of 2.8 x 10(5) cells x L(-1) and 45.0% of the total phytoplankton abundance. In the dry season, Eucampia zoodiacus became the key dominant species (5.9 x 10(4) cells x L(-1)) when it was 43.47% of the total phytoplankton abundance. Distribution of the dominant species varied with salinity of sea-water, and their amounts correlated negatively with nutrients and zooplankton.  相似文献   

18.
We describe a dynamic model developed from a commercially available modeling package (ECoS-III) to simulate estuarine dissolved inorganic nitrogen (DIN) dynamics, and consequent N(2)O production and atmospheric flux on the timescale of tidal cycles. Simulated model state variables were NH(4)(+), NO(3)(-) and N(2)O concentrations, and salinity. Model outputs were evaluated through comparison with summer field data for the Tyne estuary, UK. The model adequately reproduced the observed axial profiles of NH(4)(+), NO(3)(-) and N(2)O concentrations. Nitrification was shown to be the dominant N(2)O source and estimates of the ratios nitrification to DIN load and N(2)O emission to DIN load are considerably lower than the corresponding values adopted in global scale models of estuarine N(2)O emissions based on DIN transformations. Hence our results are consistent with the requirement imposed by atmospheric N(2)O growth rate constraints that the amount of atmospheric N(2)O arising from agriculturally related sources, including estuarine transformations of N, be revised downward.  相似文献   

19.
Does the Structural Quality of Running Waters Affect the Occurrence of Macrophytes? The morphological structure plays, besides e.g. nutrient concentration, an important role for the integrated assessment of running waters. This paper focuses on the relationship between structural quality and macrophyte vegetation. During summer 2000 structural quality according to LAWA, macrophyte vegetation, and selected habitat parameters were recorded in 135 mapping sections in running waters in Southern Germany. In streams of high structural diversity, generally higher species richness is expected than in rivers of low diversity of habitats. However, no significant differences in macrophyte species richness were detected between different classes of structural quality. In contrast, bryophytes alone showed a significant decrease in species richness with degradation of structural quality. The number of species of other macrophytes increased, respectively. The relationship between occurrence of macrophytes, structural quality, and other environmental variables was analysed using canonical correspondence analysis. Rivers of high structural quality mostly showed high flowing velocities and were heavily shaded. These environmental conditions, which characterize river types of mountainous regions, were predominantly tolerated by bryophytes. Vascular plants and charophytes generally prefer slowly flowing and unshaded habitats. River types exhibiting these environmental conditions often are more influenced by human activities and are more structurally degraded, respectively. With respect to these fundamental differences between river types, species richness of macrophytes and class of structural quality are not correlated when all types of rivers are taken into account. Type‐specific ecomorphological parameters, which conceal the differences in species richness caused by structural quality, are discussed. Structural degraded rivers can provide good environmental conditions for vascular plants and charophytes. To predict macrophyte species richness from structural quality, a differentiation of river types is essential.  相似文献   

20.
Sixteen sediment samples collected from eight transects in a mangrove swamp of the Jiulong River Estuary, Fujian, China were investigated for their content of polycyclic aromatic hydrocarbons (PAHs) and the biodegradation potential of the indigenous microorganisms. The bacterial community structures in the mangrove sediments and in enrichment cultures were also investigated. The results showed that the total PAHs concentration of mangrove sediments ranged from 280 to 1074 ng g(-1) dry weight, that the PAHs composition pattern in the mangrove sediments was dominated by high molecular weight PAH components (4-6 rings), and that Benzo[ghi]perylene and Indeno[1,2,3-cd]pyrene were the most dominant at different stations. Abundant PAH-degrading bacteria were found in all the stations, the values of phenanthrene-degrading bacteria ranged from 5.85 x 10(4) to 7.80 x 10(5) CFU g(-1) dry weight, fluoranthene-degrading bacteria ranged from 5.25 x 10(4) to 5.79 x 10(5) CFU g(-1) dry weight, pyrene-degrading bacteria ranged from 3.10 x 10(4) to 6.97 x 10(5) CFU g(-1) dry weight and the benzo(a)pyrene-degrading bacteria ranged from 5.25 x 10(4) to 7.26 x 10(5) CFU g(-1) dry weight. DGGE analysis of PCR-amplified 16S rDNA gene fragments confirmed that there was a remarkable shift in the composition of the bacterial community due to the addition of the different model PAH compound phenanthrene (three ring PAH), fluoranthene(four ring PAH), pyrene(four ring PAH) and benzo(a)pyrene(five ring PAH) during enrichment batch culture. Eleven strains were obtained with different morphology and different degradation ability. The presence of common bands for microbial species in the cultures and in the native mangrove sediment DNA indicated that these strains could be potential in situ PAH-degraders.  相似文献   

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