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1.
Kathryn E. Collins Catherine M. Febria Helen J. Warburton Hayley S. Devlin Kristy L. Hogsden Brandon C. Goeller 《新西兰海洋与淡水研究杂志》2019,53(2):182-200
Excessive macrophytes can cause significant problems in agricultural waterways requiring active management. Conventional control techniques can have a range of adverse effects. We investigated several control tools in two experiments: firstly, we tested eight treatments at a small-scale (2?m?×?2?m). We found intensive hand weeding, weed mat and herbicide spraying to be effective treatments, reducing macrophyte cover to <5%. Hand weeding and weed mat immediately reduced cover, while dieback from herbicide took two months. Weed mat was a novel and effective control mechanism along stream banks. Secondly, we tested the impact of shading on macrophyte growth. Macrophyte growth was enhanced under partially shaded conditions, but with 80% effective shading over the entire channel, cover was reduced to 17%. Once treatments ceased, macrophytes grew back within 3–5 months. Long-term, control methods will require combinations of tools but will need to include optimal shading for the target species. 相似文献
2.
Microbes are believed to play important roles in ecosystem function in many environments. The hot springs of Xiamen Island are close to the Xiamen Sea, and may have some characteristics different from those of inland hot springs. Microbes living in the hot springs of Xiamen may have new characteristics. However, little is known about microbial communities of hot springs close to the Xiamen Sea. A cuhure-dependent survey of microbial population in the Xiamen hot springs was pcrformed by using an approach combining total cellular protein profile identification and 16S rRNA gene sequencing. A total of 328 isolates of bacteria were obtained from liquid and sediment samples from the Xiamen hot springs, including neutrophilie thermophilic bacteria and moderately thermophilic acidophiles. Neutrophilic thermophilic bacteria, which grow at a temperature range of 55-90℃ including Rhodothermus marinus (Strain 1) , Thermus thermophilus (Strain 2), Thermus thiopara (Strain 3) , Geobacillus stearothermophilus(Strain 4) , Geobacillus thermoleovorans (Strain 5) , and Pseudomonas pseudoal-caligenes (Strain 6), were recovered by 2216E plates. Moderately thermophilic acidophiles, which can grow at temperatures above 50℃ and a pH range of 1. 8-3.5 such as Alicyclobacillus acidoterrestris (Strain 8) , Sul-fobacillus acidophilus (Strain 9), and Sulfobacillus thermosulfidooxidans (Strain 10), were isolated on selective solid medium containing sulfur and Fe2+. Among these strains, Rhodothermus marinus, Thermus thermophilus and Geobacillus stearothermophilus are not only thermophilcs, but also halophiles. One bacterium strain (Strain 6) shared 99% nucleotide sequence homology with Pseudomonas pseudoalcaligenes on the 16S rRNA gene se-quence, but was quite different from Pseudomonas pseudoalcaligenes in biological characteristics, suggesting that it may represent a novel thermophilic species. Results indicated that various species of neutrophilic thermophiles and moderately thermophilic acidophiles were widely distributed in the Xiamen hot springs and that Rhodother-mus marinus and Thermus thermophilus dominated the cultivable microbial community. 相似文献
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Microbes are believed to play important roles in ecosystem function in many environments. The hot springs of Xiamen Island are close to the Xiamen Sea, and may have some characteristics different from those of inland hot springs. Microbes living in the hot springs of Xiamen may have new characteristics. However, little is known about microbial communities of hot springs close to the Xiamen Sea. A culturedependent survey of microbial population in the Xiamen hot springs was performed by using an approach combining total cellular protein profile identification and 16S rRNA gene sequencing. A total of 328 isolates of bacteria were obtained from liquid and sediment samples from the Xiamen hot springs, including neutrophilic thermophilic bacteria and moderately thermophilic acidophiles. Neutrophilic thermophilic bacteria, which grow at a temperature range of 55—90℃ including Rhodothermus marinus (Strain 1), Thermus thermophilus (Strain 2), Thermus thiopara (Strain 3), Geobacillus stearothermophilus (Strain 4), Geobacillus thermoleovorans (Strain 5), and Pseudomonas pseudoalcaligenes (Strain 6), were recovered by 2216E plates. Moderately thermophilic acidophiles, which can grow at temperatures above 50℃ and a pH range of 1.8—3.5 such as Alicyclobacillus acidoterrestris (Strain 8), Sulfobacillus acidophilus (Strain 9), and Sulfobacillus thermosulfidooxidans (Strain 10), were isolated on selective solid medium containing sulfur and Fe2+. Among these strains, Rhodothermus marinus, Thermus thermophilus and Geobacillus stearothermophilus are not only thermophiles, but also halophiles. One bacterium strain (Strain 6) shared 99% nucleotide sequence homology with Pseudomonas pseudoalcaligenes on the 16S rRNA gene sequence, but was quite different from Pseudomonas pseudoalcaligenes in biological characteristics, suggesting that it may represent a novel thermophilic species. Results indicated that various species of neutrophilic thermophiles and moderately thermophilic acidophiles were widely distributed in the Xiamen hot springs and that Rhodothermus marinus and Thermus thermophilus dominated the cultivable microbial community. 相似文献
5.
Scientists investigating toxicants such as endocrine disrupting chemicals (EDCs) at the cellular at the sub-cellular level are often faced with criticisms as to how these effects can be extrapolated to the level of individuals and their populations. This report aims to provide an overview of the studies undertaken on crustacean model, Echinogammarus marinus LEACH (AMPHIPODA), and intersex phenotypes, at the individual and population levels, and provide additional emergent data at the genomic level. These, normal and intersex, males and females have been investigated by cross-hybridisation microarray analysis and specific sexually dimorphic genes and corresponding properties identified between each sexual phenotype. The morphology, physiology and histology of these intersexes have been investigated in detail and a number of reproductive costs have been identified including reduced fecundity and fertility. These costs have been incorporated into a population model and simulated over a ten-year period to ascertain how different levels of intersexuality affect the stability of populations. Based on the information gained through study of intersex models (with known endocrine dysfunction) together with the substantial quantity of historical data relating to effects of chemicals on amphipod fecundity, growth and mortality, the development of appropriate biomarkers is nearer to being assessed from the level of genes to that of the population. 相似文献
6.
Scarlett A Rowland SJ Canty M Smith EL Galloway TS 《Marine environmental research》2007,63(5):457-470
Acute sediment toxicity tests do not test key life stage events such as moulting and reproduction and therefore do not reveal the longer-term effects of contaminant exposure. A laboratory method is described for determining the chronic toxicity of contaminants associated with whole sediments. The test is conducted using neonates of the estuarine amphipod Corophium volutator at 15 degrees C, salinity 25 psu and a 12 h light:12 h dark photoperiod. The endpoints are survival and growth after 28 days and survival, growth and reproduction of amphipods upon termination of test i.e. reproduction within all control vessels (ca 75 days). The sediment chronic toxicity test was used to investigate the effects of sediments spiked with environmentally relevant preparations of slightly weathered Alaskan North Slope crude oil, including a water-accommodated-fraction (WAF) and a chemically-dispersed (Corexit 9527) WAF. Sediment oil concentrations were quantified using ultra-violet fluorescence. The amphipods exposed to chemically dispersed oil had higher mortality and lower growth rates than control-, Corexit 9527- and WAF-exposed organisms, resulting in reduced reproduction. The described method supplements the standard acute sediment test and would be particularly useful when long-term ecological effects are suspected but acute tests reveal no significant mortality. The sediment chronic test reported herein has shown that sediment that was not evidently toxic during 10-day acute tests could have population-level effects on sediment-dwelling amphipods. 相似文献
7.
Contaminant exposure and effects in Baltic ringed and grey seals as assessed by biomarkers 总被引：3，自引：0，他引：3
Nyman M Bergknut M Fant ML Raunio H Jestoi M Bengs C Murk A Koistinen J Bäckman C Pelkonen O Tysklind M Hirvi T Helle E 《Marine environmental research》2003,55(1):73-99
The Baltic Sea ecosystem has suffered from a heavy pollutant load for more than three decades. Persistent organic pollutants (POPs) and heavy metals have been of most concern due to their persistence and toxic properties. Ringed seals (Phoca hispida baltica) and grey seals (Halichoerus grypus) living in the Baltic Sea have been suffering from pathological impairments, including reproductive disturbances, which have resulted in a depressed reproductive capacity. We investigated several biochemical parameters as potential biomarkers for exposure to and effects of the contaminant load in the Baltic seals. Seals from less polluted areas were used as reference material in terms of the pollution load. In both Baltic seal populations, the levels of some biochemical parameters diverged from those in the reference seals, and some of these showed a clear correlation with the individual contaminant load. Of the potential bioindicators, we propose cytochrome P4501A activity and vitamin E levels, in blubber or plasma, as exposure biomarkers for polychlorinated biphenyls (PCBs) and dichlorodiphenyltrichloroethane (DDT) in both species. The arylhydrocarbon receptor-mediated chemical-activated luciferase gene expression (CALUX) response reflects the whole PCB and DDT burden in ringed seals. Retinyl palmitate (vitamin A) levels showed a negative correlation with the individual POP load, and is proposed as potential effect biomarkers for the depletion of the vitamin A stores. As the nutritional levels of both vitamin A and E have an impact on the vitamin levels in the seals, more information on the dietary vitamin levels is needed before any conclusions can be drawn. As the relationship between biochemical parameters and contaminants varied between the two species, species-specific characteristics has to be considered when monitoring the health status and possible toxic effects of the contaminant load in ringed and grey seals. 相似文献
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We have developed polyclonal and, recently, monoclonal antibodies against the aromatic hydrocarbon-inducible P450 1A1 form purified from Atlantic cod (Gadus morhua). A simple, indirect enzyme-linked immunosorbent assay (ELISA) based on these antibodies has been developed in our laboratory and tested in numerous experiments with both field-collected and laboratory-exposed fish of different species. The exposure situations studied to date include complex mixtures such as polychlorinated biphenyls (PCBs), dioxins and water-soluble oil fractions, as well as defined compounds such as CB congeners, TCDD, and pesticides. Factors affecting the induction response, including sexual maturation and dietary factors, have also been investigated. The ELISA technique generally shows good correlation with contaminant exposure and catalytic measurements, but has also given new and important information in certain cases where catalytic measurements failed to reveal effects. 相似文献
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随着除草剂在农业生产中的大量使用, 近岸海域中除草剂被频繁检出。除草剂的残留会对海草床生态系统的结构与功能产生潜在的威胁。本文回顾了近40年来除草剂对海草胁迫的相关研究工作, 总结发现:(1)光系统Ⅱ(PSⅡ)型除草剂在全球海草床中被频繁检出, 是研究最多的除草剂种类。(2)不同种类海草对不同类型除草剂耐受程度不同, 多种除草剂混合物可能会对海草表现出协同、加性或拮抗效应。(3)除草剂会与其他因素如高温、光照、重金属等共同对海草产生胁迫作用。(4)叶绿素荧光技术结合现代组学技术可以更加灵敏、全面地阐释除草剂对海草的胁迫机理。最后对未来的研究方向提出了展望, 包括开展对重点海草床中除草剂残留的长期监测, 加强对新型除草剂、海草的不同生活史阶段以及多因素联合胁迫作用的研究。本文有助于全面了解海草与除草剂胁迫之间的关系, 为将来开展更深入的研究工作提供参考。 相似文献
10.
From sediment bioassay to fish biomarker--connecting the dots using simple trophic relationships. 总被引：1，自引：0，他引：1
C A Rice M S Myers M L Willis B L French E Casillas 《Marine environmental research》2000,50(1-5):527-533
Two common problems in applying and interpreting invertebrate bioassays and fish biomarkers in sediment toxicology are the wide gap between significant effects concentrations determined by these two approaches, and a general lack of ecological context. We have devised an exposure system that is able to reconcile much of the disparity between invertebrate bioassay and fish biomarker results by incorporating realistic ecological processes based on deposit feeding and predator-prey interactions. This system relates the disturbance of interest (sediment contamination) to biologically meaningful effects in a resource of interest (marine flatfish) via a realistic contaminant vector (a deposit-feeding polychaete worm). In this pilot study, polychaetes (Armandia brevis) were exposed for 28 days to clean sediments supplemented with benzo(a)pyrene (BaP), para-para dichlorodiphenyldichloroethylene (pp'DDE), Aroclor 1254, or field sediments collected from two sites in Puget Sound, Washington, contaminated predominantly with polcyclic aromatic hydrocarbons (PAHs) or chlorinated compounds. Exposed worms were then fed live to juvenile English sole (Pleuronectes vetulus) for 10 or 12 days. At the end of the exposure period, fish were measured for length and weight, sacrificed, and preserved for either routine histopathology and immunohistochemical analysis of cytochrome P450 1A induction, or 32P post-labeling determination of hepatic PAH-DNA adducts. Growth of predatory flatfish was lower than reference in all but one of eight groups fed contaminant-exposed polychaetes; however, statistically significant reductions in growth were only observed in three of these eight groups, at least in part due to low statistical power. Juvenile sole from all contaminant-exposed groups showed increased expression of CYP1A, and fish exposed to BaP-exposed worms showed clear evidence of hepatic PAH-DNA adducts. This method allows the concurrent evaluation of sediment contamination at multiple biological and ecological levels. These results indicate that sediments determined to be nontoxic by common invertebrate bioassays may have the potential to cause adverse effects at higher trophic levels. 相似文献
11.
Identification of Perkinsus-like parasite in Manila clam, Ruditapes philippinarum using DNA molecular marker at ITS region 总被引：1，自引：0，他引：1
ZHANG Xichang LIANG Yubo FAN Jingfeng ZHANG Wei PU Hongyu LIANG Bin CHEN Hongxing SONG Lichao 《海洋学报(英文版)》2005,24(5):139-144
Genomic DNA was extracted from hypnospores of Perkinsus-like parasite of Manila clam Ruditapes philippinarum collected at the fishing grounds in Huanghai Sea coast Shicheng Island and East China Sea coast Ningbo, China. The internal transcribed spacer（ITS） in rDNA was PCR-amplified, cloned, sequenced, and compared with that of five Perkinsus species in GenBank. The fragment amplified from DNA of parasite of either Shicheng Island or Ningbo contained 649 bp, including partial ssrRNA（51 bp） and ITS（＋5.8 S） （598 bp） regions. The ITS（＋5.SS） sequences of Perkinsus-like parasite of both Shicheng Island and Ningbo were all 99% identical to those ofPerkinsis atlanticus, and were not more than 95% identical to those of other four Perkinsus species including P. marinus, P. andrewsi, P. qugwadi and P. medierraneus.The ITS （＋5.8S） sequence of Perkinsus-like parasite of Shicheng Island was 99% identical to that of Ningbo. These facts about nucleotide sequences suggested that the Perkinsus-like parasite in Manila clam, Ruditapes philippinarum collected from either the Huanghai Sea coast or the East China Sea coast was P. atlanticus, and might reflect P. atlanticus strains of distinct geographic distribution. 相似文献
12.
孔石莼与2种海洋微藻的胞外滤液交叉培养研究 总被引：5，自引：0，他引：5
在实验室条件下,采用海洋大型海藻石莼(Ulva pertusaKjellm.)和海洋微藻青岛大扁藻(Platymonas helgolandicaKylin var.tsingtaoensis)、亚历山大藻(Alexandrium tamarense(Lebour)Balech)的滤液做了交叉培养研究,初步探索藻间的克生机制。试验结果表明,不同温度处理下,2种微藻滤液对石莼生长的影响均有显著的差异。不同温度处理的石莼滤液对青岛大扁藻生长的影响差异显著,而对亚历山大藻生长的影响差异不显著。可以推测:石莼对亚历山大藻的抑制作用很可能是通过细胞的直接接触来完成的;而石莼对青岛大扁藻的抑制作用和两种微藻对石莼的抑制作用主要是通过分泌胞外产物(Extracellular products,ECP)来实现的,至于其它的方式和途径有待于进一步研究。 相似文献
13.
Several immune parameters were evaluated in two species of mussels (Mytilus californianus and M. galloprovincialis/M. trossulus) as bioindicators of contaminant effects. The mussels were deployed in San Francisco Bay Estuary and a control site at Bodega Marine Laboratory. Assays for phagocytosis and phagocytic index (average number of particles engulfed per hemocyte) were conducted with hemocytes in their own hemolymph-the "Serum" method. The responses were compared with contaminant concentrations in those mussels. For both species, the contaminated South Bay Dumbarton Bridge and Redwood Creek sites had elevated phagocytosis relative to the Bodega control site, indicating contaminant stress. The results also showed that M. californianus had higher percentages of phagocytosis (74%) and a higher phagocytic index (4.6 particles per cell) than those of M. galloprovincialis/M. trossulus (60% phagocytosis and 3.5 particles per cell). As there is a difference in immune response to contaminants, it is suggested that future San Francisco Estuary monitoring should be conducted with endemic M. galloprovincialis/M. trossulus rather than with the currently utilized M. californianus, which is not found in the estuary. 相似文献
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Scott T. Larned Jonathan Moores Jenni Gadd Brenda Baillie Marc Schallenberg 《新西兰海洋与淡水研究杂志》2020,54(3):551-591
ABSTRACT To meet the challenges of preventing and reversing adverse effects of land use on ecosystems, management actions need to be founded on strong evidence. We used the pressure-state-impact (PSI) framework to assess evidence of land-use effects on New Zealand freshwater ecosystems. The evidence consisted of published quantitative and categorical associations linking land-use pressures to state changes and ecological impacts in rivers, lakes and aquifers. There was substantial evidence of land-use effects, particularly where land use/land cover (LULC) classes were used as pressure variables. Proportions of catchment area in urban and pastoral LULC were consistently, positively correlated with contaminant levels in water bodies and negatively correlated with ecological-health indicators. Other consistent PSI associations included positive correlations between cattle stocking rates and river contaminant levels, increased fine sediment and decreased ecological-health scores in rivers following forest harvest, and increased river contaminant levels at sites with stock access. Despite these consistent associations, the evidence base has four general shortcomings that should be addressed: (1) inadequate integration of data and models that link land use and contaminant loss to state changes and impacts in freshwater ecosystems; (2) weak inferences based on LULC; (3) reliance on categorical PSI associations; (4) gaps in reported PSI associations. 相似文献
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Bioavailable contaminant concentrations are an important component in assessing environmental effects as they directly affect ecosystem health. Shellfish contaminant monitoring programmes have traditionally filled this requirement but are being phased out in some jurisdictions. Passive sampling devices (PSDs) have the potential to replace shellfish monitoring; however, there are still knowledge gaps to address before this can occur. This study assessed the suitability of three different PSDs in providing the required information to replace shellfish monitoring. PSDs were deployed at three historic mussel monitoring sites with different levels of urban influence in the Waitemata Harbour, Auckland, New Zealand. Contaminants of interest were urban heavy metals, plus current and emerging organic contaminants. PSDs provided extremely low detection limits and, for some contaminants, very strong correlations to shellfish. PSDs can currently complement shellfish in monitoring, but it is premature to make conclusions as to the suitability of PSDs in replacing shellfish monitoring until more information is available. 相似文献
16.
本文主要探究了东海原甲藻(Prorocentrum donghaiense)与三角褐指藻(Phaeodactylum tricornutum)之间的他感作用。在实验室模拟条件下,分别研究了添加比例为10%、40%、60%、90%、100%的三角褐指藻无藻细胞滤液的培养基对东海原甲藻生长的影响,以及相同比例的东海原甲藻无藻细胞滤液培养基对三角褐指藻生长的影响。结果表明,低浓度三角褐指藻滤液对东海原甲藻(P.donghaiense)的生长表现为促进作用,高浓度则抑制其生长,因此,不同浓度的三角褐指藻藻液对东海原甲藻生长表现出两种相反的他感作用效果;而东海原甲藻滤液对三角褐指藻(P.tricornutum)的生长没有显著影响,说明东海原甲藻并未对三角褐指藻表现出他感作用。 相似文献
17.
通过研究硝基苯和间苯二酚对海洋微藻的毒理效应,旨在提示化工废水污染对水环境破坏的严重性,揭示海洋微藻类净化废水的潜在应用。本试验用塔玛亚历山大藻(Alexandrium tamarense)、海链藻(Thalassiosira weissflogii)和绿色巴夫藻(Pavlova viridis)为供试藻种,以微藻生长量及叶绿素 a 的变化为参数,研究不同质量浓度的硝基苯和间苯二酚对3种海洋微藻的毒理效应。结果表明：硝基苯对塔玛藻和巴夫藻的生长具有一定的抑制作用,质量浓度为12mg/L时抑制率分别为22.9%和20.04%,但对海链藻的生长在一定程度上有促进作用。间苯二酚对海链藻和巴夫藻的生长具有抑制作用,质量浓度为12mg/L 时其抑制率分别为26.11%和23.6%,对塔玛亚历山大藻的生长有一定的促进作用。对塔玛亚历山大藻和巴夫藻而言,在硝基苯质量浓度为3mg/L 时,叶绿素 a 含量均达到最低,其叶绿素 a含量分别为对照组的68.6%和50.9%,对于海链藻,硝基苯对其叶绿素a的影响波动不大。间苯二酚对3种海洋微藻叶绿素 a 含量的影响为绿色巴夫藻>海链藻>塔玛亚历山大藻,其叶绿素 a 含量分别是对照组的1.1%,24.9%,88%。 相似文献
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Andrew Swales Ron Ovenden Iain T. MacDonald Andrew M. Lohrer Karen L. Burt 《新西兰海洋与淡水研究杂志》2013,47(6):1305-1319
Abstract Concerns about the potential adverse effects of Spartina invasion on New Zealand estuaries, such as habitat loss and degradation, have led to herbicide use to control its spread. An experiment was conducted in the Manukau Harbour (New Zealand) to determine sediment remobilisation rates (?E) from herbicide‐treated S. anglica patches. Paired treatment and control patches (≤25 m diam.) were monitored at two sites 500 m apart with different wave exposures. Above‐ (AGB) and below‐ground (BGB) biomass decomposition and AE were determined from repeat surveys over c. 2.5 years. Work done by waves (W), which integrates wave‐energy dissipation at the bed over time, was used as a surrogate measure of sediment transport potential. Complete AGB loss occurred in both treated patches within 3 months of initial herbicide application, whereas BGB (a matrix of roots and rhizomes) persisted much longer. An exponential decay model (r 2 = 0.6) fitted to the site‐two treatment data predicted an c. 85% reduction in BGB after 5 years, whereas the BGB changes for the larger and more heterogeneous site‐one treatment were more complex. BGB was a better predictor of average bed elevation than AGB in multiple regression models and, although bed elevation decreased through time and with waves, BGB was positively related to bed elevation at both sites. AE was similar for both treatment patches (c. 0.05 and c. 0.06 m yr‐1, P < 0.001, r2 ≥ 0.97) and complete sediment loss was predicted to occur within 6–10 years. The site‐one control patch accumulated sediment (0.01 m yr‐1 , P < 0.001, r 2 = 0.81) whereas the more wave‐exposed site‐two control eroded (0.01 m yr‐1, P = 0.0017, r 2 = 0.61). The potential for adverse sediment effects resulting from Spartina control at wave‐exposed sites will be minimal if the treated areas are small relative to the size of the estuary. 相似文献
20.
Nitrogen and phosphorus in New Zealand streams and rivers: Control and impact of eutrophication and the influence of land management 总被引：2，自引：0，他引：2
Abstract Given sufficient light and heat, the growth of aquatic macrophytes and algae associated with eutrophication is generally controlled by the concentration, form and ratio between nitrogen (N) and phosphorus (P). Data from 1100 freshwater sites monitored for the last 10 years by New Zealand's regional councils and unitary authorities were assessed for streams and rivers with mean nitrate/ nitrite‐N (NNN), dissolved reactive P (DRP), total N (TN) and total P (TP) concentrations in excess of New Zealand guidelines, and to generate a data set of N:P ratios to predict potential periphyton response according to the concentration of the limiting nutrient. The frequency of sites exceeding the guidelines varied from 0 to 100% depending on the parameter and region, but South Island regions were generally more compliant. The dissolved inorganic N (DIN) to dissolved reactive P (DRP) ratio was used to group data into three nutrient limitation classes: <7:1 (N‐limited), between 7:1 and 15:1 (co‐limited), and>15:l (P‐limited), by mass. P‐limitation was the most frequent scenario in New Zealand streams (overall, 76% of sites were P‐limited, 12% N‐limited, and 12% co‐limited). The mean concentration of the limiting nutrient for each site was combined with empirical relationships to predict periphyton densities (the average of N‐and P‐limited growth was used for sites with co‐limitation). This assessment predicted that 22 sites were likely to exceed the periphyton guideline for protecting benthic biodiversity (50 mg chlorophyll a m?2), but this assessment is likely to be highly changeable in response to climatic conditions and present and future land use. As an example, we modelled N and P losses from an average sheep and a dairy farm in Southland (South Island, New Zealand) in 1958, 1988, 2008 and 2028. We predicted that with time, as farm systems have and continue to intensify, N losses increase at a greater rate than P losses. Since the pathway for N to reach fresh waters may be more tortuous and take longer than P to reach a stream or river, focusing mitigation on P losses may have a quicker effect on potential algal growth. In addition, with time, it is expected that P‐limitation in New Zealand's rivers and streams will be more widespread as N‐losses are unabated. Hence, although strategies to decrease N losses should be practised, mitigating P losses is also central to preventing eutrophication. 相似文献