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1.
通过对神狐海域表层5个沉积物样品进行脂肪酸组成及其碳同位素分布特征测试和研究,结果表明总脂肪酸含量分布为5.14~8.99 μg/g,碳数分布范围从C12到C32,类型包括正构饱和脂肪酸、支链脂肪酸、单不饱和脂肪酸、多不饱和脂肪酸和类异戊二烯酸等计48种;样品中饱和的短链脂肪酸主要来自细菌与浮游生物,而长链饱和脂肪酸来自陆源高等植物,其比例表明研究海域中海洋细菌与浮游生物输入量远远大于陆源高等植物的输入。支链脂肪酸10me16:0与单不饱和脂肪酸18:1ω9、16:1ω9主要来自硫酸盐还原菌,而单不饱和脂肪酸16:1ω7很大可能来源于硫氧化菌,比较相对含量得出神狐海域表层沉积物为还原环境。样品中检测出8种类异戊二烯酸,主要是植烷酸和17,21-ββ二升藿烷酸,及少量的姥鲛酸,推测为叶绿素a和细菌微生物的共同贡献。  相似文献   

2.
Variation in the microbial biomass and community structure found in sediment of heavily polluted bays and the adjacent unpolluted areas were examined using phospholipid fatty acid analysis. Total microbial biomass and microbial community structure were responding to environmental determinants, sediment grain size, depth of sediment, and pollution due to petroleum hydrocarbons. The marker fatty acids of microeukaryotes and prokaryotes - aerobic, anaerobic, and sulfate-reducing bacteria -were detected in sediments of the areas studied. Analysis of the fatty acid profiles revealed wide variations in the community structure in sediments, depending on the extent of pollution, sediment depth, and sediment grain size. The abundance of specific bacterial fatty acids points to the dominance of prokaryotic organisms, whose composition differed among the stations. Fatty acid distributions in sediments suggest the high contribution of aerobic bacteria. Sediments of polluted sites were significantly enriched with anaerobic bacteria in comparison with clean areas. The contribution of this bacterial group increased with the depth of sediments. Anaerobic bacteria were predominantly present in muddy sediments, as evidenced from the fatty acid profiles. Relatively high concentrations of marker fatty acids of sulfate-reducing bacteria were associated with organic pollution in this site. Specific fatty acids of microeukaryotes were more abundant in surface sediments than in deeper sediment layers. Among the microeukaryotes, diatoms were an important component. Significant amounts of bacterial biomass, the predominance of bacterial biomarker fatty acids with abundance of anaerobic and sulfate-reducing bacteria are indicative of a prokaryotic consortium responsive to organic pollution.  相似文献   

3.
To estimate the source and diagenetic state of organic matter reaching bottom sediments, fatty acids and sterols were measured in unconsolidated surface material (flocs) at 12 sites ranging from 600 to 2000 m across the mid-Atlantic continental slope off Cape Hatteras, North Carolina. Total free and esterefied fatty acids were similar in distribution and concentration to other coastal systems, with values ranging from 0.64 to 46.52 μg mg−1 organic carbon (1.10–68.85 μg g−1 dry sediment). Although shallow (600 m) stations contained significantly greater fatty acid concentrations than deep (> 1400m) stations, high variability observed at mid-depth (800 m) collections precluded a consistent relationship between total fatty acid concentration and station depth. At three sites where underlying sediments were also collected, decreases in total fatty acids, reduced amounts of polyenoic acids and significant presence of bacterial fatty acid suggest rapid reworking of labile organic material that reaches the sediment surface. The distribution of sterols was remarkably consistent among all sites even though there were large variations in concentrations (1.8–20.7 μg mg−1 organic carbon). Sterol composition indicated phytoplankton, principally diatoms and dinoflagellates, as the principal source of labile organic matter to sediments, together with a significant input of cholest-5-en-3β-ol typical of zooplankton and their feeding activity. A minor but widespread terrigenous input was also evident based upon significant concentrations of sterols dominant in vascular plants.  相似文献   

4.
Sediment traps were deployed at 5 depths of 100 through 5,250 m to collect suspended sediments in the northern North Pacific (47°51.1'N; 176°20.6'E, 5,300 m deep) in the summer of 1978. Fatty acid composition was determined in the samples of phytoplankton, particulate matter, trap sediment and bottom sediment.Fatty acid composition of the trap sediments revealed no significant vertical trend throughout the water column from depths of 100 to 5,250 m, and were also similar to those of the phytoplankton and the particulate matter from the euphotic layer. However, a marked difference in the fatty acid composition was observed between the trap sediments and the particulate matter from deep waters. Therefore, it can be concluded that the source of fatty acids in the trap sediments is the particulate matter from the euphotic layer but not from deep waters.Unsaturated fatty acids highly susceptible to biological agents were rather abundant in the trap sediments as well as in the phytoplankton and particulate matter from the euphotic layer, however no unsaturated fatty acid was found in the particulate matter from deep waters. From these findings, it is clear that the particulate matter of the euphotic layer is transported to deep waters very rapidly. As the sinking rate of fecal pellets produced by zooplankton is in the range of ten to hundreds of meters a day, fecal pellets are assumed to be the most likely carrier of rapid-transport of organic matter including fatty acids from the euphotic layer to deep waters.  相似文献   

5.
海水和淡化养殖凡纳滨对虾的组织成分比较研究   总被引:7,自引:0,他引:7  
对海水(盐度为28)和淡化(盐度为2)条件下养殖8周的凡纳滨对虾体营养成分蛋白质、脂肪、灰分、矿物质、氨基酸以及脂肪酸组成等进行了比较研究.2种盐度养殖的全虾粗蛋白与粗脂肪含量没有显著差异,但淡化养殖的全虾灰分含量显著降低.从2种盐度养殖的虾壳、肝胰腺、肌肉与血清等组织的矿物元素含量来看,淡化养殖对对虾的组织矿化程度造成一定的影响.淡化养殖的对虾血清蛋白含量下降,而肝胰腺指数却显著上升.2种盐度养殖的对虾肌肉氨基酸总量与脂肪酸总量没有显著差异,但淡化养殖的对虾肌肉必需氨基酸含量上升,而不饱和脂肪酸含量略微下降.结果表明,盐度对对虾各组织成分的影响是对虾对不同盐度的生境进行渗透调节与离子调控的适应性反应.  相似文献   

6.
To examine the source and preservation of organic matter in the shelf sediments of the East China Sea (ECS), we measured bulk C/N and isotopes, organic biomarkers (n-alkanes and fatty acids) and compound-specific (fatty acids) stable carbon isotope ratios in three sediment cores collected from two sites near the Changjiang Estuary and one in the ECS shelf. Contrasting chemical and isotopic compositions of organic matter were observed between the estuarine and shelf sediments. The concentrations of total n-alkanes and fatty acids in the shelf surface sediments (0–2 cm) were 5–10 times higher than those in estuarine surface sediments but they all decreased rapidly to comparable levels below the surface layer. The compositions of n-alkanes in the estuarine sediments were dominated by C26-C33 long-chain n-alkanes with a strong odd-to-even carbon number predominance. In contrast, the composition of n-alkanes in the shelf sediment was dominated by nC15 to nC22 compounds. Long-chain (>C20) fatty acids (terrestrial biomarkers) accounted for a significantly higher fraction in the estuarine sediments compared to that in the shelf sediment, while short-chain (<C20) saturated and unsaturated fatty acids were more abundant in the shelf surface sediments than in the estuarine sediments. Stable carbon isotopic ratios of individual fatty acids showed a general positive shift from estuarine to shelf sediments, consistent with the variations in bulk δ 13CTOCTOC. These contrasts between the estuarine and shelf sediments indicate that terrestrial organic matter was mainly deposited within the Changjiang Estuary and inner shelf of ECS. Post-depositional diagenetic processes in the surface sediments rapidly altered the chemical compositions and control the preservation of organic matter in the region.  相似文献   

7.
The membrane phospholipids of two barophilic bacteria, DB21MT-2 and DB21MT-5, isolated from sediments from the Marianas Trench at 11,000 m were structurally characterized and quantitatively determined by liquid chromatography/electrospray ionization/mass spectrometry (LC/ESI/MS). Phospholipids detected in the barophiles were distributed in five different classes: phosphatidylglycerol (PG), phosphatidylinositol (PI), phosphatidylcholine (PC), diphosphatidylglycerol (DPG), and phosphatidylethanolamine (PE) and its methylated forms phosphatidylmethylethanolamine (PME) and phosphatidyldimethylethanolamine (PDME). Concentrations of phospholipids/phospholipid groups range from 5.3 to 4.583 μg/g (dry weight). A unique aspect of the phospholipid profiles of the barophilic bacteria was the wide distribution of polyunsaturated fatty acids 20 : 5 (in DB21MT-2) and 22 : 6 (in both DB21MT-5 and DB1MT-2) on the sn-1 and mostly on the sn-2 position of the phospholipids. The results suggest that the adaptation of the barophiles to low temperature and high hydrostatic pressure influenced the synthesis of phospholipids containing polyunsaturated fatty acids. It was also observed that the polyunsaturated fatty acids were associated with almost every phosphatidylglycerol (PG) molecule. Presumably, the larger head group of PG would be expected to cause greater disruption in acyl chain packing within the membrane bilayer and thereby lower the transition temperature in response to the additive effects of low temperature and high pressure. The detection of phospholipids with polyunsaturated fatty acids also has important geochemical implications for paleoenvironment reconstruction and for determining the surface water biological productivity of the ocean. It seems that psychrophilic and barophilic bacteria may be major sources of polyunsaturated fatty acids to the deep-sea sediments, given the fact that the vertical flux of polyunsaturated fatty acids from surface water plankton decreased rapidly with depth.  相似文献   

8.
During the spring plankton bloom (1976) in the northern North Sea the fatty acid and lipid distribution of the chloroform extractable fraction of seawater was analyzed by gas chromatography and thin-layer chromatography. The fatty acids in the seawater made up about 3% of the total dissolved organic matter. Maximum concentrations during the first phytoplankton bloom were in the range of 5 μmol C dm?3. Palmitic acid (30.4%) and oleic acid (21.4%) as well as myristic, stearic and palmitoleic acids were the main fatty acids. The concentrations of fatty acids were higher below the thermocline in the deeper layers of the water column. The oleic acid showed large fluctuations, especially below the thermocline and seemed to be bound in a polar fraction. Different lipid classes containing fatty acids were determined by thin-layer chromatography. An estimation showed that the free fatty acid fraction made up the main portion (47.9%), followed by the triacylglycerol fraction (22.8%) and a more polar fraction with 25.1%. The triacylglycerol fraction appears to be more stable than the other fraction in the water column.  相似文献   

9.
Microbial community structure in sediments, as determined by the phospholipid ester-linked fatty acid (PLFA) analysis, collected from Kojima Lake during summer and winter was described with reference to the environmental quality of the lake. A characteristic feature of the study area was the persistence of anoxia in summer and winter in the deeper area, and the sediments were under the reduced condition with high levels of sulfide, chemical oxygen demand, and low values of oxidation-reduction potential. PLFA profiles of the sediment were dominated by the saturated, branched and monounsaturated fatty acids. Small amounts (less than 5% of the total PLFA) of polyunsaturated fatty acids (biomarker fatty acids of microeukaryotes) and long chain fatty acids were detected in sediments. Total PLFA concentrations in sediments were higher in summer than in winter. Microbial community structure in the lake sediments was predominated by the prokaryotes, as evidenced from the larger amounts of bacterial biomarker fatty acids in the range of C10 to C20. The presence of signature fatty acids of sulfate reducing bacteria in sediments was consistent with the high levels of sulfide in the sediment and anoxic condition in the study area. PLFA showed significant differences in PLFA profiles between shallow and deeper areas, indicating the differences in the lipid contributing communities. However, no such a significant difference was observed between summer and winter.  相似文献   

10.
Total hydrolysable amino acids (THAA), individual amino acid distributions, total organic carbon (TOC) and total nitrogen (TN) were measured in sediments across the Goban Spur continental slope at water depths of 651, 1296 and 3650 m. Objectives were to examine (1) differences in organic matter (OM) degradation state in surface sediments across the slope from sedimentary amino acid compositions, and (2) whether these differences are related to particle size distributions. Application of a ‘reaction–diffusion’ model to the sediment concentration profiles showed that TOC and THAA degradation rate constants decreased with increasing water depth. Ratios of degradation rate constants of THAA over TOC indicated that THAA turn over faster than TOC at 651 and 1296 m water depth only. From estimates of degradation rate constants of individual amino acids, it was concluded that with increasing water depth fewer amino acids contribute to overall THAA degradation. The contribution of THAA to TOC mineralisation decreased from the upper to the lower slope. Since at all three sampling stations the amino acids with the highest relative contribution to THAA had a higher abundance in sediments with reduced THAA mineralisation rates, we conclude that the overall amino acid reactivity decreases with increasing water column depth. A principal component analyses, carried out on normalised amino acid mole percentages, established significant shifts in amino acid compositions and confirmed that (1) OM degradation state increased from 651 to 3650 m and (2) that OM in the finest fraction at the shallowest station appeared to be considerably less degraded than in the coarser fractions or any size fractions at the deeper stations. Therefore, we conclude that downslope transport, sorting and accumulation of fine particles with continuous mineralisation of OM attached to the particles during vertical and lateral transport results in an increasing organic matter degradation state from the upper slope to the abyssal plain.  相似文献   

11.
分析了浙江南部近海(东海陆架泥质区)沉积物柱状样中游离态和碱不稳定结合态脂类生物标志物的组成.沉积物中的脂肪酸呈现以C16脂肪酸为主峰的单峰形分布趋势,具明显偶碳优势,除表层沉积物中脂肪酸以游离态为主外,结合态为柱状样中脂肪酸的主要存在形态,脂肪酸的垂直分布主要由降解作用控制;以甾醇和脂肪醇为主体的醇酮组分主要以游离态...  相似文献   

12.
Bacterial communities of different respiratory types were isolated from a marine sediment in a multiple chemostat system in an attempt to obtain bacterial cultures representative of the sediment. The fatty acid distribution of a mixed culture of sulphate-reducing bacteria isolated from this system showed a good correlation with the lipid distribution of the zone of maximum sulphate-reduction activity within the sediment. Both distributions had significant concentrations of C14 : 0, iso and anteiso C15 : 0, C16 : 0, C16 : 1ω7 and C18 : 1ω7. This was in contrast to the lipid profile of Desulfovibrio desulfuricans obtained by batch enrichment, which was dominated by iso C17 : 1ω7 fatty acids and correlated poorly with the sediment. The bacterial community cultured from the sediment was further differentiated according to respiratory types (aerobic, facultative aerobic and facultative anaerobic) by growth in a chemostat under defined conditions. The fatty acid distributions of these communities were sufficiently different to enable the various respiratory types to be recognized by their particular fatty acid composition. Cyclopropyl fatty acids (?17 and ?19) were present in significant levels only in the aerobic bacteria, while the facultative aerobes had significantly higher C18 : 1ω7 than the other cultures and the facultative anaerobic community was the only culture to have significant amounts of C12 : 0. The fatty acid distribution of Loch Eil sediment over the range C12-19 seemed to be predominantly of bacterial origin and were relatively abundant to a depth of 6 cm. The concentration of cyclopropyl fatty acids was highest in the oxidized surface sediments and decreased with depth as anaerobic conditions began to dominate, indicating that these fatty acids may only be indicative of aerobic sedimentary bacteria. In contrast to the fatty acids characteristic of bacteria, those fatty acids in the range C20-30 attributed to a terrestrial input were found in relatively constant concentrations over the whole 0–12 cm depth profile.  相似文献   

13.
南海南部表层沉积物中生物硅的分布及其环境意义   总被引:1,自引:0,他引:1  
对南海南部25个表层沉积样进行了生物硅的测定分析,试图揭示南海南部表层沉积生物硅的分布及其对现代海洋环境的指示意义,以便为古海洋学研究提供进一步的科学依据。研究发现,表层沉积物中生物硅含量与其所处水深呈显著正相关关系,相关系数达到0.782。陆架浅水区表层沉积物中生物硅含量非常低,不能反映表层水体中硅质生物生产力情况,这可能与沉积类型和陆源物质输入影响有关。深水区表层沉积物中生物硅的含量分布表明,其不仅能反映表层水体中硅质生物的古生产力水平,而且还能指示上升流的强弱,从而进一步证实了利用沉积物中生物硅含量来追踪上升流发育和变化的有效性与可信度。研究结果还显示,在研究区域中北部表层沉积生物硅中放射虫和海绵骨针较硅藻占有更大的比重,这可能是由于硅藻易被溶解并易被其他生物体摄食的缘故。在有上升流发育的海域,放射虫、硅藻和海绵骨针基本上表现出较高的丰度,这与高的生物硅含量相一致。  相似文献   

14.
用GC/MS法分析比较了紫菜粉、小球藻、酵母、Banta液(牛粪1.5g+干稻草2g+沃土20g+水1000cm3)4种食物条件下蚤状潘的脂肪酸组成.结果表明:4种食物培养下,蚤状潘的总脂含量依次为39.80、60.10、44.60、52.30mg/g.一共检测出脂肪酸14种,其中8种饱和脂肪酸(SFA),2种单不饱和脂肪酸(MUFA),4种多不饱和脂肪酸(PUFA).紫菜粉培养组与小球藻组PUFA含量显著高于酵母组和Banta液组(P〈0.05),占总脂肪酸的1/3以上,酵母组MUFA质量分数最高(P〈0.05),达55.73%,Banta液组以SFA质量分数最高(P〈0.05),为52.45%.其中紫菜粉组的PUFA中缺乏C18:3,但C20:5(EPA)和C20:4(AA)质量分数高达22.67%和9.02%是小球藻组的1.6倍和4.5倍(P〈0.05),而且EPA和AA的实际含量也显著高于小球藻组(P〈0.05),达到9.02mg/g和3.59mg/g;比较得出,紫菜粉培养组的20碳以上必需不饱和脂肪酸含量丰富,明显高于其他3组.本实验证实蚤状潘的脂肪酸组成与食物脂肪酸成正相关.分析认为,用紫菜粉培养枝角类用于海水育苗会取得更佳的营养效果.  相似文献   

15.
南沙海洋沉积物中脂肪酸地球化学研究   总被引:10,自引:0,他引:10  
本文对南沙海洋岩芯和层样品中的脂肪酸进行了分离和鉴定,研究了脂肪酸的组成和分布特征,探讨了沉积物中有机质的来源及脂肪酸的早期成岩变化。结果表明,脂肪酸的组成和分布,反映了沉积有机质主要来自浮游生物和细菌,陆源高等植物的贡献较少;低碳及不饱和脂肪酸相对含量随埋深降低可能与化学和生物化学降解作用有关。/  相似文献   

16.
Fatty acid biomarkers were used to investigate the biogeochemistry of a former blue mussel (Mytilus edulis) aquaculture site in a shallow, sheltered cove in northeastern Newfoundland. High levels of polyunsaturated fatty acids (PUFA) in net-tow and sediment trap samples indicated a substantial phytoplankton source of organic matter, and fluctuations in specific fatty acid biomarkers reflected the changing abundances of diatoms and dinoflagellates. In comparison, sediments contained very low levels of PUFA (<15%) and were dominated by terrestrial and bacterial markers. In a separate study, blue mussels were grown at this closed site, providing the opportunity to examine the relationship between lipid supply, as recorded by net-tow and trap samples, and bivalve requirements. The average plankton fatty acid composition throughout the year agreed well with that of the adult blue mussels, suggesting that fatty acids were provided in proportions very similar to the bivalves' requirements. The fatty acid composition of the blue mussels was typical of those collected elsewhere, with PUFA proportions near 50%. However, examination of fatty acid data of plankton sampled in other areas revealed that the plankton in the current study may have been unusual in providing fatty acids at levels required by the bivalves.  相似文献   

17.
本文对广西钦州(Gq)、福建霞浦(Fx)、浙江温州(Zw)、浙江三门(Zs)、浙江宁海(Zn)、浙江慈溪(Zc)等6个野生群体拟穴青蟹(Scylla paramamosain)肌肉进行了氨基酸及脂肪酸分析。共测定出19种游离氨基酸和26种脂肪酸。氨基酸含量在80.94—191.39mg/g之间, Gq为最高;总脂肪酸含量在7.97—11.38mg/g之间,Zn为最高。主要氨基酸为甘氨酸和精氨酸;主要脂肪酸为C20:5n3(EPA),占总脂肪酸含量的平均值为(22.47±5.84)%。各群体风味氨基酸和多不饱和脂肪酸含量丰富,占总氨基酸比例(TDAA/TFAA)和总脂肪酸比例(PUFA/TFA)分别为(53.62±5.43)%和(59.27±5.29)%。Zs群体的必需氨基酸含量及C22:6n3(DHA)均为最高。主成分分析结果表明:各群体的氨基酸与脂肪酸组成与含量相似性与地理距离分布具有一定的相关性,特别是Gq群体,在氨基酸与脂肪酸的分类中与其他群体区分比较明显。各群体风味氨基酸和多不饱和脂肪酸的聚类分析结果均表明:Zw、Zc与Gq群体较为接近,具有较强的甜味; Fx、Zn与Zs群体较为接近,具有较强的香味及不饱和脂肪酸营养价值。  相似文献   

18.
脂肪酸的组成分析对认识红树植物脂肪酸资源、红树林生态系统内部的营养关系、食物网的结构,以及红树植物对生境的适应等具有重要参考价值。本研究分析了海南岛铁炉港和清澜港两个港湾的红海榄(Rhizophora stylosa)、杯萼海桑(Sonneratia alba)、木果楝(Xylocarpus granatum)、角果木(Ceriops tagal)、榄李(Lumnitzera racemosa)、木榄(Bruguiera gymnorrhiza)和正红树(Rhizophora apiculata)等7种不同红树植物叶片中脂肪酸的组成及含量。在样品中共检测出25种脂肪酸,其中月桂酸、棕榈酸和肉豆蔻酸等8种存在于所有样品中。样品中含量最高的饱和脂肪酸均为棕榈酸(占比44.32%),其次为硬脂酸(占比7.74%),不饱和脂肪酸含量较高的有油酸(占比7.61%)、亚油酸(占比9.81%)和二十碳烯酸(占比12.16%)。植物样品的脂肪酸不饱和指数为0.24~1.13。除木果楝和清澜港采集的木榄样品外,其余样品种的不饱和脂肪酸含量均低于饱和脂肪酸。铁炉港的红海榄、杯萼海桑、角果木和榄李4种植物叶...  相似文献   

19.
Results of organic carbon, total nitrogen, amino acid and hexosamine analyses of samples collected during time-series sediment trap investigations in the Arabian Sea are presented. Samples were taken over a period of years at two depths at each of three locations in the western, central and eastern part of the basin. Seasonal changes in amino acid contents and their spectral distributions show that degradation of organic matter in the water column is reduced during the monsoons, which are the high-flux periods in the western and central Arabian Sea. At the eastern site more degraded material of possibly recycled marine or terrestrial origin reaches the traps during the late summer peak fluxes. The results of hexosamine analyses suggest that bacterial biomass is relatively enriched on particles sinking in the water column and, to a larger extent, at the sediment-water interface. Decomposition between intermediate and deep water results in a loss of 30–40% of total organic carbon and more than 40% of amino acids. Comparison of the measured accumulation rates of organic carbon in sediment traps with those of organic carbon preserved in sediments show that more than 85% is lost before final burial in the sediments. Organic matter preservation in the Arabian Sea is higher than the average for the open ocean; this maybe due to the abundance of refractory organic matter of recycled marine or terrestrial origin.  相似文献   

20.
珠江口3个站位沉积柱的脂肪酸研究结果显示:沉积物中含量最高的脂肪酸是C16:0,其他脂肪酸包括不饱和脂肪酸、单甲基支链脂肪酸和长链脂肪酸。脂肪酸组成表明研究区有机质主要来源于藻类、细菌和陆源有机质。由于横琴岛附近的HQ1031站位靠近外海,初级生产力较高,因而含较多藻类脂肪酸。离淇澳岛较远的QA-B站位处于河口上缘,陆源物质丰富,初级生产力较低,因此该站位含较多陆源脂肪酸。横琴岛附近HQ1031站位的TOC值和总脂肪酸含量均大于QA-B站位,这两个站位的TOC值与总脂肪酸含量在垂向上的相关性差。横琴岛附近HQ1031站位和离淇澳岛较近的QA-A站位分别在20-22cm和16-18cm处藻类脂肪酸突然大量增加,可能与藻类输入突然增多有关。沉积物中所检测到的能指示厌氧微生物或革兰氏阳性菌的脂肪酸含量高于革兰氏阴性菌,表明珠江口沉积物微生物群落中以厌氧微生物为主。  相似文献   

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