共查询到20条相似文献，搜索用时 93 毫秒
1.
Deli YU Shufeng LIU Liyu TIAN Peiping ZHANG. College of Material Science Engineering Jilin University Changchun China 《东北亚地学研究》2008,11(3):188-190
Analyzing raw material＇s structure and performance of bentonite from Panzhihua in Sichuan, the authors think that it is adequate for agglomerant of iron smelting. According to its composition and property we have researched the purification and modification of I/S bentonite under conditions of different dispersants and sodium agent. XRD test result reveals that the essential minerals of Panzhihua bentonite are I/S mixed-layer ones, and FTIR analysis shows that when adding 1.5% sodium pyrophosphate to the bentonite slurry during purification, the composition of quartz in bentonite decreases to less than 4% and I/S is more than 90%. The optimized modification technic conditions are Na2CO3 （4%） and CMC-Na （3%） as modified agents, the clay and water are 10 vs. 1, and the temperature is 75℃. It is 40 min for stirring time and reacting time is 4 h. Under the conditions we can get the modified I/S bentonite with colloid index more than 500 nd/15 g. 相似文献
2.
【目的】以2株海洋真菌为研究对象,探究不同种类及浓度的化学表观遗传修饰剂对其次级代谢产物化学多样性和生物活性的影响。【方法】基于化学表观遗传修饰策略,采用两种不同的化学表观遗传修饰剂5-氮杂胞嘧啶核苷(5-azaC)和丁酸钠,分别对Penicillium sp.019和Aspergillus terreus ZN4-5-4两株海洋来源真菌进行表观遗传修饰。通过观察真菌在培养基中的生长状态变化,并根据薄层层析(TLC)指纹图谱、高效液相色谱(HPLC)图谱、乙酰胆碱酯酶(AChE)抑制、1,1-二苯基-2-三硝基苯肼(1,1-Diphenyl-2-picryl-hydrazyl,DPPH)自由基清除和卤虫致死活性筛选模型等技术手段,从中筛选出代谢产物丰富、AChE抑制活性与抗氧化活性产物丰富且毒性小的发酵培养条件。【结果】添加1 mmol/mL的丁酸钠诱导剂可使菌株019和ZN4-5-4的次生代谢产物的种类增加,且同时能提高该菌株次级代谢产物的AChE的抑制活性和DPPH自由基清除活性。因此,确定菌株019和ZN4-5-4的最优发酵条件均是添加浓度为1 mmol/mL丁酸钠的PDB培养基。【结论】化学表观遗传修饰策略指导下,添加诱导剂5-azaC或者丁酸钠对2株海洋真菌次生代谢产物的产生有影响。 相似文献
3.
许进 《广东海洋大学学报》2008,28(4)
研究了改性水玻璃砂的基本工艺性能,结果表明:在相同的粘结剂加入量条件下,用不同原砂所得到的型砂抗压强度大小顺序为大林砂>都昌砂>新会砂>岳阳砂;改性水玻璃18#的表面安定性最好,粘结剂加入量增多,表面安定性改善,吹气时间延长,表面安定性恶化;改性水玻璃的抗吸湿性比普通水玻璃好,尤其是改性水玻璃18#的抗吸湿性比普通水玻璃高出一倍以上,随着吹气时间的延长,抗吸湿性提高;改性水玻璃18#、A1和B2的可使用时间均满足生产要求;改性水玻璃B2的发气量最大,18#、A1的发气量略小于普通水玻璃。 相似文献
4.
蓝田膨润土系笔者首次发现,本文初步研究了该膨润土的成分、属性、物化性能及其地质特征等。经研究认为,蓝田膨润土属钙镁基土,是比较少见的类型;除含大量蒙脱石外,还含一定数量的有益组分——凹凸棒石和高岭石;物化性能尚好;沉积成因,具有一定的规模和应用价值,建议应进一步进行普查找矿和应用开发研究。 相似文献
5.
利用双阳台水管观测资料检测地球自由振荡 总被引：2，自引：2，他引：0
基于双阳台水管倾斜仪的数字化观测资料,利用功率谱密度估计方法,在没有对资料进行去固体潮处理的情况下,准确获得了2004年12月26日苏门答腊大地震激发的0S3～0S31基频球型自由振荡。并与PREM模型的理论自由振荡周期进行了对比,发现实测振荡周期与PREM预测的振荡周期相吻合,除0S3、0S4振型的观测周期和PREM模型理论周期的相对误差大于0.3%外,其他振型的观测周期和PREM模型理论周期的相对误差大都集中在0.1%左右。同时还检测到了5个谐频球型振荡和8个环型振荡。 相似文献
6.
We modified the sediment incipient motion in a numerical model and evaluated the impact of this modification using a study case of the coastal area around Weihai, China. The modified and unmodified versions of the model were validated by comparing simulated and observed data of currents, waves, and suspended sediment concentrations(SSC) measured from July 25~(th) to July 26~(th), 2006. A fitted Shields diagram was introduced into the sediment model so that the critical erosional shear stress could vary with time. Thus, the simulated SSC patterns were improved to more closely reflect the observed values, so that the relative error of the variation range decreased by up to 34.5% and the relative error of simulated temporally averaged SSC decreased by up to 36%. In the modified model, the critical shear stress values of the simulated silt with a diameter of 0.035 mm and mud with a diameter of 0.004 mm varied from 0.05 to 0.13 N/m~2, and from 0.05 to 0.14 N/m~2, respectively, instead of remaining constant in the unmodified model. Besides, a method of applying spatially varying fractions of the mixed grain size sediment improved the simulated SSC distribution to fit better to the remote sensing map and reproduced the zonal area with high SSC between Heini Bay and the erosion groove in the modified model. The Relative Mean Absolute Error was reduced by between 6% and 79%, depending on the regional attributes when we used the modified method to simulate incipient sediment motion. But the modification achieved the higher accuracy in this study at a cost of computation speed decreasing by 1.52%. 相似文献
7.
黄浦江上游水源地通管工程C3标段采用DN4000钢顶管施工,单次顶进长度达到969.94m,如何降低顶力是整个工程成功的关键。为减少摩阻力从而降低顶力,对泥浆配制、注浆控制等方面进行了研究,并结合JB06~JB05及JB07~JB06顶进段的地层、顶进力变化状况,分析顶管摩阻力的变化规律。结果表明:①该工程注浆孔的合理布置、触变泥浆的配制与使用得到的减阻效果明显,可作为类似工程减阻技术的参考;②顶力与顶进距离近似呈线性关系,但地层本身的性质会造成顶力的突变,类似工程可先对地层性质进行研究以得到更准确的顶力预测,指导顶管施工;③已注入环空一定时间后的泥浆与刚注入的泥浆相比减阻效果更好,顶力增速过快时可降低顶进速度,待泥浆充分与地层反应后再以原速顶进;④适当增加注浆量可以有效地减小管周摩阻力;⑤高聚物膨润土泥浆比膨润土泥浆单价更高,但高聚物膨润土泥浆注浆量少,综合环境效益等多方面因素考虑,高聚物膨润土泥浆更有优势。 相似文献
8.
利用线粒体16S rRNA基因和线粒体细胞色素氧化酶亚基Ⅰ(cytochrome oxidaseⅠ,COⅠ)基因片段初步研究钦州湾牡蛎(Ostrea)的物种组成。以特异引物进行PCR扩增,对扩增产物进行纯化、测序,分析表明,16S rRNA基因部分长度为413bp,COⅠ基因部分长度为535bp,2种牡蛎序列的碱基组成均显示出较高的A+T比例:16S rRNA基因59.8%;COⅠ基因60.5%。对位排序比较表明,16S rRNA片段种内个体间变异较小,存在7个变异位点,4种单倍型,其中包括5个转换位点突变,2个颠换位点突变;COⅠ片段有30个碱基存在变异,7种单倍型,其中包括16个转换位点突变,14个颠换位点突变。运用MEGA4软件计算出不同个体间的遗传距离,并构建了NJ和UPGMA系统树。香港牡蛎(Crassostrea hongkongensis)16S rRNA和COⅠ序列与白肉牡蛎的遗传距离均为0.000,有明巨牡蛎(Crassostrea ariakensis)16S rRNA和COⅠ序列和红肉牡蛎(red meat ostrea)的遗传距离分别为0.000和0.011,白肉牡蛎16S rRNA和COⅠ序列和红肉牡蛎之间的遗传距离分别为0.035和0.146。结果表明,钦州湾牡蛎分为2个不同的种,线粒体16S rRNA和COⅠ基因在种间存在明显的多态性,证实了16S rRNA和COⅠ基因序列适用于牡蛎的系统学分析。 相似文献
9.
Anti-lipopolysaccharide factors (ALFs) are important antimicrobial peptides that are isolated from some aquatic species. In a previous study, we isolated ALF genes from Chinese mitten crab, Eriocheir sinensis. In this study, we optimized the production of a recombinant ALF by expressing E. sinensis ALF genes in Escherichia coli maintained in shake-flasks. In particular, we focused on optimization of both the medium composition and the culture condition. Various medium components were analyzed by the Plackett-Burman design, and two significant screened factors, (NH4)2SO4 and KH2PO4, were further optimized via the central composite design (CCD). Based on the CCD analysis, we investigated the induction start-up time, the isopropylthio-D-galactoside (IPTG) concentration, the post-induction time, and the temperature by response surface methodology. We found that the highest level of ALF fusion protein was achieved in the medium containing 1.89 g/L (NH4)2SO4 and 3.18 g/L KH2PO4, with a cell optical density of 0.8 at 600 nm before induction, an IPTG concentration of 0.5 mmol/L, a post-induction temperature of 32.7°C, and a post-induction time of 4 h. Applying the whole optimization strategy using all optimal factors improved the target protein content from 6.1% (without optimization) to 13.2%. We further applied the optimized medium and conditions in high cell density cultivation, and determined that the soluble target protein constituted 10.5% of the total protein. Our identification of the economic medium composition, optimal culture conditions, and details of the fermentation process should facilitate the potential application of ALF for further research. 相似文献
10.
为分析评估BDS-3新信号(B1C和B2a)的定轨服务水平,收集2021-05-01~06-30全球均匀分布的69个MGEX测站观测数据对B1C和B2a信号精密定轨性能进行评估。48 h重叠弧段结果表明,使用B1C/B2a组合观测值时,BDS-3 MEO卫星轨道3D RMS值优于6 cm,径向优于2 cm,激光测距检核残差优于4 cm。此外,单BDS-3 MEO及BDS-3 MEO+Galileo两种情况下新老信号精密定轨结果表明,前者新老信号定轨精度相差不超过1 mm,定轨性能基本相当;后者B1C/B2a新信号相比于B1I/B3I老信号呈现出更优的定轨性能,其3D RMS提升0.38 cm。 相似文献
11.
Lignin serves as one of the most important molecular fossils for tracing Terrestrial Organic Matters (TOMs) in marine environment. Extraction and derivatization of lignin oxidation products (LOPs) are crucial for accurate quantification of lignin in marine sediment. Here we report a modification of the conventional alkaline cupric oxide (CuO) oxidation method, the modification consisting in a solid phase extraction (SPE) and a novel on-column derivatization being employed for better efficiency and reproducibility. In spiking blanks, recoveries with SPE for the LOPs are between 77.84% and 99.57% with relative standard deviations (RSDs) ranging from 0.57% to 8.04% (n=3), while those with traditional liquid-liquid extraction (LLE) are from 44.52% to 86.16% with RSDs being from 0.53% to 13.14% (n=3). Moreover, the reproducibility is greatly improved with SPE, with less solvent consumption and shorter processing time. The average efficiency of on-column derivatization for LOPs is 100.8% ± 0.68%, which is significantly higher than those of in-vial or in-syringe derivatization, thus resulting in still less consumption of derivatizing reagents. Lignin in the surface sediments sampled from the south of Yangtze River estuary, China, was determined with the established method. Recoveries of 72.66% to 85.99% with standard deviation less than 0.01mg/10g dry weight are obtained except for p-hydroxyben-zaldehyde. The lignin content Σ8 (produced from 10 g dry sediment) in the research area is between 0.231 and 0.587 mg. S/V and C/V ratios (1.028 ± 0.433 and 0.192 ± 0.066, respectively) indicate that the TOMs in this region are originated from a mixture of woody and nonwoody angiosperm plants; the high values of (Ad/Al) v suggest that the TOMs has been highly degraded. 相似文献
12.
基于武汉大学发布的BDS-2/3观测量偏差(OSB)改正产品,采用国内8个iGMAS测站1个月的观测数据,分析OSB改正前后对B1I/B3I旧频点及B1C/B2a新频点2种组合模式下BDS-2/3伪距单点定位(SPP)和精密单点定位(PPP)精度的影响。结果表明,B1I、B3I、B1C和B2a的OSB年均值为-80～70 ns,各频点OSB年稳定性分别为3.41 ns、5.87 ns、2.04 ns和2.32 ns。在BDS-2/3伪距单点定位方面,改正后B1I/B3I组合的3D方向定位精度优于2.53 m, B1C/B2a组合的3D方向定位精度优于3.84 m,二者精度提升均不明显。在BDS-2/3精密单点定位方面,B1I/B3I组合的3D方向定位精度优于7.7 cm,提升约20.6%,收敛时间约为38 min,提升约7.3%;B1C/B2a组合的3D方向定位精度优于3.7 cm,提升约11.9%,收敛时间约为36 min,提升约16.3%。 相似文献
13.
黄静 《中国地理科学(英文版)》1996,6(2):120-131
ＯＮ ＤＥＶＥＬＯＰＩＮＧ ＥＮＶＩＲＯＮＭＥＮＴ ＡＮＤ ＲＥＳＯＵＲＣＥ ＤＥＶＥＬＯＰＭＥＮＴ ＯＦ ＰＡＮＺＨＩＨＵＡ ＨｕａｎｇＪｉｎｇ（黄静）（ＲｅｇｉｏｎａｌＥｃｏｎｏｍｉｃＩｎｓｔｉｔｕｔｅ，Ｐｅｏｐｌｅ’ｓＵｎｉｖｅｒｓｉｔｙｏｆＣ... 相似文献
14.
The seaweed Sargassum hemiphyllum is widely distributed throughout the coastal waters of Asia and has high commercial value.In recent years,its natural biomass has declined due to over-exploitation and environmental pollution.To seek for a feasible way to culture this seaweed efficiently,we designed a simple long-line system in a shrimp pond for the culture during winter,and the growth and nutritional composition of the seaweed were examined.Results show that the culture system was durable and flexible allowing S.hemiphyllum to grow vertically off the muddy bottom of the pond.Although the length of pondcultured S.hemiphyllum was inhibited by water depth,the weight-specific growth rate((1.65±0.17)%/d) was nearly three times higher than that of wild plants((0.62±0.19)%/d).The crude protein(6.92%±0.88%) and ash content(21.52%±0.07%) of the pond-cultured seaweed were significantly lower than those of the wild plants(9.38%±0.43% and 26.93%±0.07%,respectively);however,crude fat(1.01%±0.04%) was significantly higher than that of the wild plants(0.87%±0.02%).In addition,the nutritional composition of both pond-cultured and wild S.hemiphyllum was comparable to or even higher than those of other common seaweeds being used as food and/or aquaculture fodder.Future studies shall be focused on the impact of environmental parameters on its growth and nutritional composition. 相似文献
15.
《中国海洋大学学报(英文版)》2021,20(5)
The mitochondrial genomes(mitogenomes) are purportedly under selection for smaller size to improve their replication and translation efficiency. However, the mitogenomes of Arcidae species are larger than those of other bivalves, and are among the largest metazoan mitogenomes reported to date. In order to explore the differences of base composition and selective constraints between the large and small mitogenomes, we compared the mitogenomes of 9 large arcid mitogenomes and 77 small bivalves mitogenomes. Base composition analyses indicated that Arcidae mitogenomes have significantly greater GC skews in both their whole genomes and coding sequences. This result suggests that the replication of the large mitogenomes in Arcidae may be slower than those in other bivalves, exposing the parental strand to deamination for a longer time. Selection pressure analyses showed that the mitochondrial protein-coding genes of Arcidae species have significantly higher Ka/Ks ratios than other bivalves,suggesting that they have accumulated more nonsynonymous nucleotide substitutions. Seven protein-coding genes(atp6, cox1-3,nad1, nad4 and nad5) show significant difference for Ka/Ks ratios between the Arcidae and non-Arcidae groups. However, these divergences are not observed in the nuclear gene within histone H3. From these observations, we concluded that the large mitogenomes of Arcidae species experienced more relaxed selective constraints. As some Arcidae species are more tolerant to hypoxia that can lead to low metabolic rate, the relaxed selective constraints of mitogenomes may be energy-related. This study provides new insights into the evolution of Arcidae mitogenomes. 相似文献
16.
下石炭统天鹅坪组页岩是湘中地区页岩气勘探的新层系。基于野外地质调查、页岩气钻井的样品采集和分析测试等对湘中涟源凹陷石炭系天鹅坪组泥页岩的发育特征、有机地球化学特征、储层特征和含气性等页岩气成藏地质条件进行了分析。结果表明:涟源凹陷石炭系天鹅坪组有机质丰度较高,w（TOC）分布在0.78%~2.84%之间,有机质类型以Ⅱ1型为主,有机质成熟度Ro分布在1.48%~2.09%之间,处于高成熟演化阶段;泥页岩矿物成分以石英和黏土矿物为主,黏土矿物主要由伊蒙混层、伊利石和绿泥石组成;泥页岩有机质孔较发育,孔隙度平均为1.97%,渗透率平均为0.847×10-3 μm2,但大多小于0.1×10-3 μm2;湘新地4井天鹅坪组泥页岩含气量分布在1.64~4.29 m3/t,平均2.69 m3/t（不含残余气）。结合区域构造-沉积演化背景的分析表明,涟源凹陷下石炭统天鹅坪组页岩气成藏条件的主控因素为沉积环境、构造保存条件,其中深水陆棚相是页岩气有利相带,复杂构造背景下由对冲式构造样式形成的向斜对页岩气保存条件最为有利,是研究区页岩气勘探的"甜点区"。研究成果可以为该区后续油气勘探提供参考。 相似文献
17.
Algal biotechnology has advanced greatly in the past three decades. Many microalgae are now cultivated to produce bioactive substances. Odontella aurita is a marine diatom industrially cultured in outdoor open ponds and used for human nutrition. For the first time, we have systematically investigated the effects of culture conditions in cylindrical glass columns and fiat-plate photobioreactors, including nutrients （nitrogen, phosphorus, silicon, and sulfur）, light intensity and light path, on O. aurita cell growth and biochemical composition （protein, carbohydrate, β-1,3-glucan, lipids, and ash）. The optimal medium for photoautotrophic cultivation of O. aurita contained 17.65 mmol/L nitrogen, 1.09 mmol/L phosphorus, 0.42 mmol/L silicon, and 24.51 mmol/L sulfur, yielding a maximum biomass production of 6.1-6.8 g/L and 6.7-7.8 g/L under low and high light, respectively. Scale-up experiments were conducted with fiat-plate photobioreactors using different light-paths, indicating that a short light path was more suitable for biomass production of O. aurita. Analyses of biochemical composition showed that protein content decreased while carbohydrate （mainly composed of 15-1,3-glucan） increased remarkably to about 50% of dry weight during the entire culture period. The highest lipid content （19.7% of dry weight） was obtained under 0.11 mmol/L silicon and high light conditions at harvest time. Fatty acid profiles revealed that 80% were Cx4, C~6, and C20, while arachidonic acid and eicosapentaenoic acid （EPA） accounted for 1.6%-5.6% and 9%-20% of total fatty acids, respectively. High biomass production and characteristic biochemical composition profiles make O. aurita a promising microalga for the production ofbioactive components, such as EPA and D-1,3-glucan. 相似文献
18.
硝酸银络合法浓缩金枪鱼鱼油多不饱和脂肪酸的研究 总被引：6，自引：2，他引：4
以金枪鱼鱼油混合脂肪酸为材料 ,利用硝酸银络合法浓缩其中的多不饱和脂肪酸 (PU FA) ,主要考察了搅拌时间和硝酸银浓度对产品得率和EPA和DHA含量的影响 ,并测定了产品的理化指标和脂肪酸组成。研究结果表明 :搅拌时间为 6 0min ,硝酸银浓度为 4mol/L时得到的产品得率为 18.0 3% ,浓缩产品中的EPA和DHA的质量分数分别为 9.78%和 86 .0 7% ,总质量分数达95 .85 % ,过氧化值为 7.35mmol/kg ,碘值 2 6 0 8.9g/kg。 相似文献
19.
在大菱鲆(Scophthalmus maximus)工厂化养殖废水中接种繁茂膜海绵(Hymeniacidon perleve),并将繁茂膜海绵与大菱鲆同池养殖,研究繁茂膜海绵对大菱鲆工厂化养鱼水体微生物的净化作用。结果表明,接种量为7 mg/L繁茂膜海绵经过8、16、24、32、40、48 h对养鱼废水中细菌总数的清除率分别为23.18%、47.65%、66.09%、81.57%、93.80%、98.96%;对弧菌的清除率分别为48.61%、56.44%、78.13%、92.88%、97.10%、98.56%;对大肠杆菌的清除率分别为39.51%、41.90%、73.24%、92.29%、97.69%、99.14%,将繁茂膜海绵以7 mg/L的生物量与大菱鲆同池养殖24 h,水体中细菌总数、弧菌和大肠杆菌的清除率分别为61.38%、50.34%、93.47%。 相似文献
20.
Yongxiang Yu Zheng Zhang Yingeng Wang Meijie Liao Bin Li Liangyi Xue 《中国海洋大学学报(英文版)》2017,16(5):831-839
Novel preservation condition without ultra-low temperature is needed for the study of pathogen in marine fishes. Freeze-drying is such a method usually used for preservation of terrigenous bacteria. However, studies using freeze-drying method to preserving marine microorganisms remain very limited. In this study, we optimized the composition of protectants during the freeze-drying of Edwardsiella tarda, a fish pathogen that causes systemic infection in marine fishes. We found that the optimal composition of protectant mixture contained trehalose (8.0%), skim milk (12.0%), sodium citrate (2.0%), serum (12.0%) and PVP (2.0%). Orthogonal and interaction analyses demonstrated the interaction between serum and skim milk or sodium citrate. The highest survival rate of E. tarda was observed when the concentration of NaCl was 10.0, 30.0 and between 5.0 and 10.0 g L?1 for preparing TSB medium, E. tarda suspension and protectant mixture, respectively. When E. tarda was frozen at ?80°C or ?40°C for 6 h, its survival rate was higher than that under other tested conditions. Under the optimized conditions, when the protectant mixture was used during freeze-drying process, the survival rate (79.63%–82.30%) of E. tarda was significantly higher than that obtained using single protectant. Scanning electron microscopy (SEM) image indicated that E. tarda was embedded in thick matrix with detectable aggregation. In sum, the protectant mixture may be used as a novel cryoprotective additive for E. tarda. 相似文献