共查询到20条相似文献，搜索用时 500 毫秒
1.
Jonathan D. Istok 《Ground Water Monitoring & Remediation》2013,33(3):61-68
In situ bioremediation is being considered to optimize an existing pump‐and‐treat remedy for treatment of explosives‐contaminated groundwater at the Umatilla Chemical Depot. Push‐pull tests were conducted using a phased approach to measure in situ hexahydro‐1,3,5‐trinitro‐1,3,5‐triazine (RDX) and 2,4,6‐trinitrotoluene (TNT) degradation rates associated with various carbon substrates. Phase I included short‐duration transport tests conducted in each well to determine dilution rates and retardation factors for RDX and TNT. Phase II included aquifer “feedings” conducted by injecting 150 gallons of treated site groundwater amended with ethanol, corn syrup, lactose or emulsified oil (concentrations 10, 25 and 27 mM, respectively; 12% by volume for emulsified oil). Wells received up to 6 substrate “feedings” over the course of 3 months followed by monitoring dissolved oxygen, nitrate, Fe(II), and sulfate to gauge in situ redox conditions as indicators of anaerobic microbial activity. Phase III included push‐pull tests conducted by injecting 150 gallons of site groundwater amended with approximately 1000 µg/L RDX, 350 µg/L TNT, carbon substrate and a conservative tracer, followed by sampling over 8 d. Corn syrup resulted in the best RDX removal (82% on average) and the largest RDX degradation rate coefficient (1.4 ± 1.1 d?1). Emulsified oil resulted in the best TNT removal (99%) and largest TNT degradation rate coefficient (5.7 × 10?2 d?1). These results will be used to simulate full‐scale in situ bioremediation scenarios at Umatilla and will support a go/no‐go decision to initiate full‐scale bioremediation remedy optimization. 相似文献
2.
The performance of one pilot‐scale and two full‐scale membrane bioreactors (MBR) were evaluated based on the control of main operational parameters, composition of microbial community and pathogens concentration in the treated outlet. Plants were designed for 0.75 m3/day (A), 60 m3/day (B) and 30 m3/day (C). Inlet and outlet samples were monitored for chemical oxygen demand (COD), biological oxygen demand, total suspended solids, ammonia nitrogen concentration (NH4–N), nitrate nitrogen concentration, total Kjeldahl nitrogen, total phosphorus and phosphate phosphorus concentration concentrations. Plants showed good COD removal: 91.9% for Plant A, 97.8% for Plant B and 94.2% for Plant C. The targeted nitrogenous ion was NH4–N due to the requirements for outlet limits. NH4–N removal was moderate for Plant A (73.3%) and Plant B (86.1%) and excellent for Plant C (>99%). Excellent phosphorus removal was achieved by Plant A (average outlet concentration was 0.7 mg/L, efficiency 84.7%). Unsatisfactory results for phosphorus removal were achieved at the full‐scale plants due to operational problems. The dependency between the extracellular polymeric substances increase and decreasing mixed liquor volatile suspended solids for both lab and full‐scale plants was confirmed. Soluble microbial product concentrations were reduced by 65–68% after coagulant dosage for Plant A. Outlets from the MBR plants were monitored for the presence of pathogens (thermotolerant coliforms, Escherichia coli, intestinal Enterococci and culturable microorganisms at 22 and 37°C). The treated effluent from Plant A, B and C met Czech national legislation regarding reuse criteria (standards) for environment, irrigation and swimming purposes. Plants B and C were not able to achieve requirements for potable water and personal hygiene quality standards. 相似文献
3.
Brett R. Baldwin Dora Taggart Yunzhou Chai David Wandor Anita Biernacki Kerry L. Sublette John T. Wilson Claudia Walecka‐Hutchison Camillo Coladonato Bryan Goodwin 《Ground Water Monitoring & Remediation》2017,37(2):58-70
Adaptive site management and aggressive bioremediation in the source zone of a complex chlorinated dense nonaqueous phase liquid (DNAPL) site reduced total chlorinated hydrocarbon mass discharge by nearly 80%. Successful anaerobic bioremediation of chlorinated hydrocarbons can be impaired by inadequate concentrations of electron donors, competing electron acceptors, specific inhibitors such as chloroform, and potentially by high contaminant concentrations associated with residual DNAPL. At the study site, the fractured bedrock aquifer was impacted by a mixture of chlorinated solvents and associated daughter products. Concentrations of 1,1,2,2‐tetrachloroethane (1,1,2,2‐TeCA), 1,1,2‐trichloroethane (1,1,2‐TCA), and 1,2‐dichloroethane (1,2‐DCA) were on the order of 100 to 1000 mg/L. Chloroform was present as a co‐contaminant and background sulfate concentrations were approximately 400 mg/L. Following propylene glycol injections, concentrations of organohalide‐respiring bacteria including Dehalococcoides and Dehalogenimonas spp. increased by two to three orders of magnitude across most of the source area. Statistical analysis indicated that reaching volatile fatty acid concentrations greater than 1000 mg/L and depleting sulfate to concentrations less than 50 mg/L were required to achieve a Dehalococcoides concentration greater than the 104 cells/mL recommended for generally effective reductive dechlorination. In a limited area, chloroform concentrations greater than 5 mg/L inhibited growth of Dehalococcoides populations despite the availability of electron donor and otherwise appropriate geochemical conditions. After implementing a groundwater recirculation system targeting the inhibited area, chloroform concentrations decreased permitting significant increases in concentrations of Dehalococcoides and vinyl chloride reductase gene copies. 相似文献
4.
为探究东南丘陵山区深水水库中生态浮床技术深度脱氮的效率及管理措施,以大型山谷型深水水库千岛湖为例,选取湿生植物空心菜(Ipomoea aquatica)和水生植物粉绿狐尾藻(Myriophyllum aquaticum),采用生态浮床技术,开展了原位模拟实验对比研究,探究不同营养盐浓度和光强下两种植物的生长状况与氮素去除效率。结果发现:(1)空心菜长势受营养盐和光照条件影响明显,添加氮磷后(TN=2.37 mg/L,TP=0.046 mg/L)的空心菜生物量是原位水体(TN=0.66 mg/L,TP=0.028 mg/L)的1.6倍,适当遮光有助于浮床植物生长,40%遮光条件下空心菜的生物量是不遮光条件下的1.5倍;而粉绿狐尾藻生长受营养盐和光照条件影响均较小。(2)空心菜对于水体氮素净化能力显著高于粉绿狐尾藻,在最佳条件下空心菜和粉绿狐尾藻对氮素的去除效率分别达到213.30和44.23 mg/(m2·d)。(3)空心菜去除氮主要以植物同化作用为主,占70%以上TN去除量,40%遮光环境通过明显提升空心菜同化吸收氮量和根系反硝化速率增强了氮的去除能力;粉绿狐尾藻同化吸收和反硝化脱氮作用各占50%左右,以遮光75%下脱氮效果最好。本研究表明,采用生态浮床技术能够强化深水水库的脱氮能力,空心菜更适合在氮浓度较高的水体生长,夏季为空心菜和粉绿狐尾藻浮床分别进行40%和75%的遮光处理将有更好的水质净化效果。因此,在滨岸湿地匮乏的深水水库实施多种植物搭配的生态浮床技术强化水体脱氮作用是一种行之有效的水质改善方法。 相似文献
5.
Bioremediation and toxicity determination of natural seawater polluted with weathered crude oil by salt-tolerant consortia in a SBR 总被引：1，自引：0，他引：1
The aim of this research was to compare the bioremediation treatment of seawater polluted with two different concentrations of weathered crude oil (0.2 for experiment (a) and 1.14% for experiment (b), v/v) by salt-tolerant consortia enriched from the sludge of a refinery wastewater treatment facility, in a SBR. The use of a commercial bioremediation stimulant (S200) was also evaluated as an alternative to the traditional nitrogen and phosphorus supplement. 相似文献
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Sulfur removal using adsorption requires a proper process parametric study to determine its optimal performance characteristics. In this study, response surface methodology was employed for sulfur removal from model oil (dibenzothiophene; DBT dissolved in iso‐octane) using commercial activated carbon (CAC) as an adsorbent. Experiments were carried out as per central composite design with four input parameters such as initial concentration (C0: 100–900 mg/L), adsorbent dosage (m: 2–22 g/L), time of adsorption (t: 15–735 min), and temperature (T: 10–50°C). Regression analysis showed good fit of the experimental data to the second‐order polynomial model with coefficient of determination R2‐value of 0.9390 and Fisher F‐value of 16.5. The highest removal of sulfur by CAC was obtained with m = 20 g/L, t = 6 h, and T = 30°C. 相似文献
7.
为更好地研究贵州红枫湖的水质变化情况,本文利用贵阳市两湖一库环境保护监测站2009-2018年对红枫湖7个代表性监测点的营养盐、叶绿素a（Chl.a）浓度和水温、气温、透明度、降雨量等水文气象条件逐月监测数据,分析红枫湖10年间水体营养盐和Chl.a浓度以及部分水文气象条件的变化趋势.运用综合营养状态指数（TLI）对红枫湖营养状态进行评价,采用Pearson相关系数法统计分析10年内Chl.a浓度与总磷（TP）、总氮（TN）等水化学组成及水位、气温等水文气象条件的相关性.结果表明,2009-2018年红枫湖水体逐月TN浓度有较大波动（0.56~2.80 mg/L）,春、夏季高于秋、冬季;水体逐月TP浓度为0.016~0.103 mg/L,夏季略高于冬季;逐月氨氮（NH3-N）浓度为0.007~0.71 mg/L,春季 > 冬季 > 秋季 > 夏季;水体逐月Chl.a浓度呈季节性波动（0.8~38.9 mg/m3）,夏季 > 春季 > 秋季 > 冬季,年内先上升后下降.TP、NH3-N、Chl.a浓度整体呈下降趋势,10年间水质有很大改善.经计算红枫湖在这10年间处于中营养状态至轻度富营养状态,且营养状态指数呈逐年下降趋势,夏季TLI明显高于其他季节.统计分析表明,红枫湖水体Chl.a浓度与高锰酸盐指数、NH3-N、TP浓度均呈显著正相关,与氮磷比呈显著负相关,与水温、pH、降雨量、气温、日照时数呈显著正相关,与透明度、气压呈显著负相关,与水位、湿度、风速无显著相关关系.表明这10年来红枫湖水体Chl.a浓度不仅受营养盐浓度控制,很大程度上还受控于气象和水文条件. 相似文献
8.
A start‐up study for biohydrogen production from palm oil mill effluent (POME) is carried out in a pilot‐scale up‐flow anaerobic sludge blanket fixed‐film reactor (UASFF). A substrate with a chemical oxygen demand (COD) of 30 g L?1 is used, starting with molasses solution for 30 days and followed by a 10% v/v increment of POME/molasses ratio. At 100% POME, a hydrogen content of 80%, hydrogen production rate of 36 L H2 per day, and maximum COD removal of 48.7% are achieved. Bio‐kinetic coefficients of Monod, first‐order, Grau second‐order, and Stover‐Kincannon kinetic models are calculated to describe the performance of the system. The steady‐state data with 100% POME shows that Monod and Stover‐Kincannon models with bio‐kinetic coefficients of half‐velocity constant (Ks) of 6000 mg COD L?1, microbial decay rate (Kd) of 0.0015 per day, growth yield constant (Y) of 0.786 mg volatile suspended solids (VSS)/mg COD, specific biomass growth rate (μmax) of 0.568 per day, and substrate consumption rate of (Umax) 3.98 g/L day could be considered as superior models with correlation coefficients (R2) of 0.918 and 0.989, respectively, compared to first‐order and Grau's second‐order models with coefficients of K1 1.08 per day, R2 0.739, and K2s 1.69 per day, a = 7.0 per day, b = 0.847. 相似文献
9.
实验设计中试水槽装置,模拟研究2种沉水植物轮叶黑藻(Hydrilla nerticillata)和苦草(Vallisneria natans)对农村水体的净化效果,并探讨沉水植物在水体氮、磷去除中的作用.结果显示,沉水植物系统均能显著降低水体中总氮、铵态氮、硝态氮和磷酸盐浓度,去除率分别达到50.5%、84.4%、41.9%和64.6%,且轮叶黑藻组选择20 g/m2,苦草组选择40 g/m2为适种密度.水体流经各串联单元时氮、磷去除负荷存在显著差异,其中总氮、硝态氮、磷酸盐浓度在有植物单元高于无植物单元,铵态氮浓度相反,结合单元内溶解氧浓度、硝化与反硝化细菌总数表明,有植物单元可显著发生反硝化作用,无植物单元可显著发生硝化作用,有无植物串联单元更有利于氮的去除. 相似文献
10.
白洋淀沉积物氮磷赋存特征及其内源负荷 总被引：11，自引：2，他引：9
白洋淀环境整治对区域生态文明建设具有重要意义,然而,目前对加剧白洋淀富营养化的内源氮、磷污染负荷依然缺乏系统的研究.本研究以野外调研和室内培养实验相结合形式,在白洋淀主要水域内采集原位柱状沉积物样品,详细地研究了白洋淀沉积物中氮、磷赋存形态、间隙水中氮、磷剖面特征以及沉积物-水界面氮、磷交换特征.结果表明,白洋淀沉积物总氮、总磷含量分别为1230.8~9559.0 mg/kg（均值2379.5 mg/kg）和344.4~915.4 mg/kg（均值608.4 mg/kg）,氮、磷累积污染量大.沉积物中铵态氮赋存量大（3.2~175.8 mg/kg）,由此导致间隙水中铵态氮浓度较高,最高达到28.8 mg/L.沉积物磷形态以Ca-P和Fe-P为主,分别占总量的38.3%~76.1%和3.98%~18.0%,间隙水中磷酸盐浓度已接近甚至高于国内外典型富营养湖区.间隙水中高浓度的铵态氮和磷酸盐导致沉积物-水界面氮、磷交换通量较高,铵态氮平均释放和扩散通量分别为106.37和12.42 mg/（m2·d）;磷酸盐平均释放和扩散通量分别为15.06和2.33 mg/（m2·d）,沉积物内源氮、磷污染负荷较高,已严重威胁到白洋淀水环境质量,迫切需要整治.其中,北部河口区域以及中部府河入湖区和人口密集活动区沉积物氮、磷内源负荷尤为突出,应成为白洋淀沉积物内源污染整治的关键区域. 相似文献
11.
氮、磷浓度对太湖水华微囊藻(Microcystis flos-aquae)群体生长的影响 总被引：5，自引：2，他引：3
大量微囊藻群体的形成和聚集是微囊藻水华形成的重要条件.氮、磷浓度是影响微囊藻群体生长的重要因素之一.为了探讨氮、磷浓度对微囊藻群体生长的影响,本研究以太湖微囊藻水华优势种之一的水华微囊藻作为研究对象,开展了不同氮、磷浓度对水华微囊藻群体生长的影响研究.以近几年太湖微囊藻水华暴发最严重的梅梁湾氮磷比的平均值作参考,氮、磷浓度设置为5个水平组,依次是T1(TN=0.1 mg/L,TP=0.005 mg/L)、T2(TN=1 mg/L,TP=0.05 mg/L)、T3(TN=10 mg/L,TP=0.5 mg/L)、T4(TN=100 mg/L,TP=5 mg/L)和T5(TN=250 mg/L,TP=5.44 mg/L)(BG-11培养基中氮、磷的浓度).结果显示,T1、T2、T3和T4 4组微囊藻群体均增大,且都发现有大于100个细胞的群体形成,群体大小分别为151、217、437和160 cells,而T5组微囊藻群体实验初期增大,实验后期变小,T5整个实验期间未发现有大于100个细胞的群体形成.研究结果表明相对低的氮、磷浓度有利于水华微囊藻群体的生长,而过高的氮、磷浓度则会抑制微囊藻群体生长.本研究结果也表明目前太湖氮、磷浓度有利于水华微囊藻群体的生长,从而有利于微囊藻水华形成. 相似文献
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Violaine Ponsin Amélie Chablais Julien Dumont Olivier Radakovitch Patrick Höhener 《Ground Water Monitoring & Remediation》2015,35(2):30-38
The objective of this study was to investigate whether 222Rn in groundwater can be used as a tracer for light non‐aqueous phase liquid (LNAPL) quantification at a field site treated by dual‐phase LNAPL removal. After the break of a pipeline, 5 ha of soil in the nature reserve Coussouls de Crau in southern France was contaminated by 5100 m3 of crude oil. Part of this oil seeped into the underlying gravel aquifer and formed a floating oil body of about 3.9 ha. The remediation consists of plume management by hydraulic groundwater barriers and LNAPL extraction in the source zone. 222Rn measurements were performed in 21 wells in and outside the source zone during 15 months. In uncontaminated groundwater, the radon activity was relatively constant and remained always >11 Bq/L. The variability of radon activity measurements in wells affected by the pump‐and‐skim system was consistent with the measurements in wells that were not impacted by the system. The mean activities in wells in the source zone were, in general, significantly lower than in wells upgradient of the source zone, owing to partitioning of 222Rn into the oil phase. The lowest activities were found in zones with high non‐aqueous phase liquid (NAPL) recovery. LNAPL saturations around each recovery well were furthermore calculated during a period of high groundwater level, using a laboratory‐determined crude oil–water partitioning coefficient of 38.5 ± 2.9. This yielded an estimated volume of residual crude oil of 309 ± 93 m3 below the capillary fringe. We find that 222Rn is a useful and cheap groundwater tracer for finding zones of good LNAPL recovery in an aquifer treated by dual‐phase LNAPL removal, but that quantification of NAPL saturation using Rn is highly uncertain. 相似文献
15.
水库水体污染控制及富营养化防治是保障城市原水供应安全的重要环节.本研究针对金泽水源水库太浦河来水氮、磷浓度较高的特点,自行设计建设了5个面积均为240 m~2的生态净化模拟试验池(A、B、C、D和对照)开展模拟试验,研究了不同水库形态、水生植物种植面积比例及种植方式对水体氮、磷污染物去除的影响.结果表明,通过模拟试验池的生态净化,原水中铵态氮(NH_4~+-N)、总磷(TP)和总氮(TN)的平均去除率分别为50.36%、53.73%和22.25%,C池TN和NH_4~+-N去除率最高分别达到了24.97%和54.61%,D池TP去除率最高,达到62.16%,水体溶解氧(DO)平均浓度提高了1.11 mg/L,平均透明度提高了27.6 cm,均显著高于对照池.水库形态结构、水生植物面积比例及种植方式对水体氮、磷污染物净化效果影响明显,增大水库浅水区面积能有效提高对水体氮、磷污染物的去除能力,增加水生植物种植面积能有效提高水体氮污染物去除和DO、透明度的提升能力,采用浮床种植方式能有效提高水体磷污染物去除和透明度提升能力.本研究结果能为金泽水源水库及其他类似水库的设计和建设提供科学依据. 相似文献
16.
Kailas L. Wasewar Pradeep Kumar Shri Chand Bina N. Padmini Tjoon Tow Teng 《洁净——土壤、空气、水》2010,38(7):649-656
The present study was aimed at removing cadmium ions from aqueous solution through batch studies using adsorbents, such as, granular activated carbon (GAC) and activated clay (A‐clay). GAC was of commercial grade where as the A‐clay was prepared by acid treatment of clay with 1 mol/L of H2SO4. Bulk densities of A‐clay and GAC were 1132 and 599 kg/m3, respectively. The surface areas were 358 m2/g for GAC and 90 m2/g for A‐clay. The adsorption studies were carried out to optimize the process parameters, such as, pH, adsorbent dosage, and contact time. The results obtained were analyzed for kinetics and adsorption isotherm studies. The pH value was optimized at pH 6 giving maximum Cd removal of 84 and 75.2% with GAC and A‐clay, respectively. The adsorbent dosage was optimized and was found to be 5 g/L for GAC and 10 g/L for A‐clay. Batch adsorption studies were carried out with initial adsorbate (Cd) concentration of 100 mg/L and adsorbent dosage of 10 g/L at pH 6. The optimum contact time was found to be 5 h for both the adsorbents. Kinetic studies showed Cd removal a pseudo second order process. The isotherm studies revealed Langmuir isotherm to better fit the data than Freundlich isotherm. 相似文献
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Variations in phosphorus speciation in two sets of simulated riparian zones with and without Perennial ryegrass were compared. Each set consisted of four units, each measuring 700 mm × 200 mm × 200 mm, which were enhanced with 0, 2.5, 5, and 7.5% red mud (RM) by weight. The levels of total phosphorus (TP), total dissolved phosphorus (TDP), soluble reactive phosphorus (SRP) in the effluent were analyzed, and phosphorus fractionation in the media were also determined after the systems had been operational for 3 months. The results showed that the unit received 2.5% RM had the highest rate of phosphorus removal, including TP, TDP, SRP, particulate phosphorus (PP), and dissolved organic phosphorus (DOP) were present at the average concentrations of 0.17, 0.10, 0.07, 0.08, and 0.03 mg/L in the effluent. Sequential phosphorus fractionation showed that calcium‐bound phosphorus (Ca–P) was the major component, indicating that the addition of RM induced aluminum/iron‐bound phosphorus (Al/Fe–P), which was intensely bioactive, to form intractable Ca–P, which further inhibited the release of phosphorus from the media. However, the presence of P. ryegrass had little effect on the removal of phosphorus. Therefore, RM, when used directly in riparian zones at a suitable concentration, is a novel and low cost additive material that can be used to remove phosphorus from reclaimed water. 相似文献
18.
淮南采煤塌陷湖泊浮游植物优势种的营养动力学 总被引：3，自引：1，他引：2
在淮南潘谢矿区选取3个营养盐结构差异较大的塌陷湖泊,于2014—2015年4个季度分别对浮游植物群落结构组成进行调查,选取3个湖泊中的优势种(属)具尾蓝隐藻(Chroomonas caudata)、链形小环藻(Cyclotella catenata)和伪鱼腥藻(Pseudanabaena sp.)作为研究对象,设置不同的氮(N)、磷(P)浓度梯度进行营养动力学培养实验,并结合Monod方程,获得3个藻种在不同营养盐限制下的营养动力学参数.N限制下具尾蓝隐藻、小环藻和伪鱼腥藻的最大生长速率(μmax)和半饱和常数(Ks)分别为:0.66 d~(-1)、1.66 mg/L;0.37 d~(-1)、1.06 mg/L;0.71 d~(-1)、2.26 mg/L;P限制下它们的μmax和Ks则分别为:0.51 d~(-1)、0.023 mg/L;0.31 d~(-1)、0.035 mg/L;0.90 d~(-1)、0.015 mg/L.综上所述,在N充足时,伪鱼腥藻能够在竞争中形成优势,同时在P限制情况下易成为优势种,从营养动力学的角度揭示了其在塌陷湖泊中占据优势的营养盐动力学机制.研究结果可以为沉陷区水域开发利用和营养盐管理提供科学依据. 相似文献
19.
为了探究生物质炭作为生长基质对水生植物种子萌发和生长的影响,选用典型沉水植物苦草(Vallisneria spiralis)作为受试植物,测定不同氮、磷营养盐浓度和不同粒径的生物质炭作用下对苦草种子萌发率、幼苗形态与生物量,同时分析水体中硝态氮、亚硝态氮、氨氮和正磷酸盐磷浓度变化过程.结果表明:生物质炭存在使水体中亚硝态氮浓度低于检测限,使水体正磷酸盐磷浓度上升至1.28~2.43 mg/L,为最高添加磷浓度的3.2~6.1倍,从而改变了苦草生长环境.小粒径生物质炭(0.25~0.5 mm)组中水体最终氨氮浓度(0.05 mg/L)远远低于大粒径生物质炭(1~2 mm)组中水体最终氨氮浓度(0.39~0.85 mg/L),即生物质炭粒径大小会影响水体最终营养盐浓度和氮素赋存形态.与大粒径生物质炭组和石英砂对照相比,小粒径组苦草种子萌发率明显升高,可达80%以上,并促进苦草幼苗生长.因此,小粒径生物质炭能提高苦草种子萌发和幼苗生长,在大型水生植物恢复工程中具有一定的应用前景. 相似文献
20.
Mamilla Prathima Devi Motakatla Venkateswar Reddy Asha Juwarkar Ponnapalli Nageswara Sarma Srinivasula Reddy Venkata Mohan 《洁净——土壤、空气、水》2011,39(10):900-907
Ex‐situ bioremediation of real‐field crude petroleum sludge was evaluated to elucidate the role of co‐culture (bioaugmentation) and external nutrients supplementation (biostimulation) under anaerobic microenvironment. Maximum removal of total petroleum hydrocarbons (TPH) was observed by integrating biostimulation with bioaugmentation (R5, 44.01%) followed by bioaugmentation alone (R4, 34.47%), co‐substrate supplemented operations [R6, 23.36%; R3, 16.5%; R2, 9.88%] and control (R1, 4.36%). Aromatics fraction showed higher degradation in all the conditions studied. Fate of six selected polycyclic aromatic hydrocarbons (PAHs) was evaluated during bioremediation. Among these, four ring PAHs compounds showed good degradation by integration of biostimulation with bioaugmentation (R5) while bioaugmentation alone (R4) documented good degradation of three ring PAHs. Lower ring PAHs compounds showed good degradation with the application of biostimulation (R6). Fluorescent in situ hybridization (FISH) detected the presence of known PAHs degrading microorganisms viz., Bacillus, Pseudomonas, Acido bacteria, Sulphur reducing bacteria Firmicutes, etc. Application of biostimulation and bioaugmentation strategies alone or in combinations documented noticeable influence on the degradation of petroleum sludge. 相似文献