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1.
Zn2+浓度对固氮鱼腥藻(Anabaena azotica Ley)光能转化特性的影响 总被引：8，自引：0，他引：8
本实验对在不同Zn^2 浓度条件下培养的固氮鱼腥藻（Anabaena azotica Ley）的生长、光合放氧速率和叶绿素荧光参数Fv/Fm进行了测定。结果表明，当Zn^2 浓度为1.0μmol/L时，其比生长速率（Specifie growth rate）最大，光合放氧速率和Fv/Fm值最高，当Zn^2 浓度大于等于5.0μmol/L是会抑制A.azotica Ley的生长和光合作用，对在0μmol/L和5.0μmol/L Zn^2 发生反应的藻蓝蛋白溶液的可见光吸收光谱的分析，发现前者624nm处藻胆体的吸收峰和后者620nm处藻蓝蛋白的吸收峰都因Zn^2 的作用而下降，推测藻蓝蛋白为Zn^2 影响光合作用的位点之一，碳酸酐酶活性的测定表明Zn^2 的浓度水平会影响其活性大小，推测是Zn^2 影响光合作用的另一途径。 相似文献
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为了研究重金属镉对藻类光合系统毒性效应及能量分配特征,以拟柱孢藻(Cylindrospermopsis raciborskii)为研究对象,分析了不同镉浓度下拟柱孢藻的生长、单位叶绿素a含量、光合系统Ⅱ(PSⅡ)叶绿素荧光及其能量分配模型.结果表明:不同浓度镉培养拟柱孢藻96 h后,0.2 mg/L Cd~(2+)对拟柱孢藻单位细胞叶绿素a含量及PSⅡ活性无显著影响,而0.5mg/L Cd~(2+)培养时拟柱孢藻的单位细胞叶绿素a含量为对照组的62.97%.当Cd~(2+)浓度高于5 mg/L时,拟柱孢藻的PSⅡ活性受到抑制并导致表征量子产额分配的φEo和ψo分别下降了15.35%和13.52%.此外,当Cd~(2+)浓度高于5 mg/L时,藻体PSⅡ能量分配表现为TRO/CSm和ETO/CSm显著减小,DIO/CSm差异不显著,类囊体能量分配表现为ABS/RC、TRO/RC和DIO/RC显著增大,明显区别于0.2、0.5和1 mg/L Cd~(2+)处理组.这些结果表明PSⅡ反应中心是镉对拟柱孢藻光合作用影响的重要作用位点,且Cd~(2+)高于5 mg/L时显著影响了拟柱孢藻PSⅡ反应中心并且抑制了PSⅡ电子传递链供体侧的电子传递,而拟柱孢藻则可能通过改变PSⅡ的能量分配来保护和缓解镉对其光合结构的损伤. 相似文献
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温度、光照强度及硝酸盐对拟柱孢藻(Cylindrospermopsis raciborskii N8)生长的影响 总被引：4，自引：3，他引：1
以从南亚热带水库中分离的拟柱孢藻(Cylindrospermopsis raciborskii N8)为材料,研究了不同温度(12、16、20、24、28、32℃)、光照强度(6.6、12.4、21.5、30.7、62.9、106.4μmol/(m2·s))和硝态氮浓度(0.5、1、2、4、8、16、32、64、128 mg/L)下拟柱孢藻的生长特性.结果表明:在实验设置的温度范围(16~32℃)内拟柱孢藻能够正常生长;最适温度范围为24~28℃,在28℃条件下,具有最大比生长速率,为0.189 d-1;当温度为12℃时,拟柱孢藻叶绿素a浓度一直降低,显著低于其他温度组(16~32℃).在6.6~106.4μmol/(m2·s)光照强度范围内,拟柱孢藻均呈指数增长趋势,最适光照强度为30.7μmol/(m2·s),其比生长速率达到最大值,为0.156 d-1;高光照强度(62.9~106.4μmol/(m2·s))下拟柱孢藻的比生长速率显著大于低光照强度(6.6~12.4μmol/(m2·s))处理组.拟柱孢藻开始指数增长的最低硝态氮浓度为4 mg/L;硝态氮浓度为8 mg/L时,拟柱孢藻达到最大比生长速率(0.155 d-1);当硝态氮浓度高于16 mg/L时比生长速率增加不显著.高硝态氮浓度组(16~128 mg/L)拟柱孢藻的叶绿素a浓度和比生长速率显著高于低硝态氮浓度组(0.5~2 mg/L).研究结果说明拟柱孢藻对温度、光照和氮源均有较宽的生态位,有利于在较大空间尺度上进行扩散. 相似文献
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以巢湖优势种铜绿微囊藻(Microcystis aeruginosa)和鱼腥藻(Anabaena sp.)为研究对象,研究不同温度(35、25和10℃)对这两种藻生长特性和胞外有机物产生的影响.结果表明,温度对铜绿微囊藻和鱼腥藻的藻细胞密度、碱性磷酸酶活性和胞外有机物浓度影响显著.25℃是铜绿微囊藻和鱼腥藻最适宜的生长温度,最高细胞密度分别达到3.12×107cells/ml和2.03×107 cells/ml.不同温度下两种藻的碱性磷酸酶活性特征,证实了高温对鱼腥藻生长的抑制和低温对铜绿微囊藻生长的抑制.胞外有机物释放总量受蓝藻生物量和单位细胞有机物释放速率的影响.铜绿微囊藻的溶解性有机碳和胞外总多糖释放量在25℃最高,最大值分别为49.28和38.46 mg/L;而鱼腥藻在35℃时释放量最高,最大值分别为45.82和40.60 mg/L;10℃条件抑制了两种藻的生长及胞外有机物的释放.鱼腥藻胞外多糖含量在35℃培养条件下最高,而铜绿微囊藻在10℃条件下最高,说明不利的生长条件会促进蓝藻胞外多糖的分泌.三维荧光图谱分析结果表明,铜绿微囊藻和鱼腥藻胞外有机物以类蛋白质和类腐殖酸为主,温度主要影响藻细胞胞外有机物浓度,而对有机物种类组成没有影响. 相似文献
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珠江流域东江干流浮游植物叶绿素a时空分布及与环境因子的关系 总被引：7，自引：2，他引：5
为了解珠江流域东江干流水体叶绿素a的时空分布及与环境因子的关系,于2012年6月(丰水期)和12月(枯水期)对东江干流进行采样调查分析.结果表明,东江水体叶绿素a含量具有明显的时空分布特征,其全年变化范围为0.84~14.93μg/L,整体均值为3.60±2.45μg/L,丰水期叶绿素a含量显著高于枯水期;而丰、枯水期叶绿素a含量空间分布特征相似,上游河段显著低于下游河段.相关性与主成分分析结果显示,水体中总氮浓度、总磷浓度、有机物含量、水温和水流流速等都是影响东江浮游植物生长的重要因素,其中以总磷的影响最为显著,表明磷可能是东江浮游植物生长的限制因子. 相似文献
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椭圆小球藻( Chlorella ellipsoidea )对4种重金属的耐受性及富集 总被引：28，自引：1，他引：28
分离筛选获得了一株高重金属抗性的椭圆小球藻 (Chlorellaellipsoidea) ,并研究了不同浓度的重金属铜、锌、镍、镉对该藻生长和叶绿素a含量的影响及其对重金属离子的吸收富集作用 .结果显示 ,该藻对Zn2 +和Cd2 +具有很高的耐受性 .对四种重金属的耐受能力依次为锌 >镉>镍 >铜 .其叶绿素a含量与重金属离子浓度呈明显负相关 .该藻对重金属具有很好的去除效果 ,经 15μmol/LCu2 +、30 0 μmol/LZn2 +、10 0 μmol/LNi2 +、30 μmol/LCd2 +浓度 72h处理 ,去除率分别达到 4 0 .93%、98.33%、97.6 2 %、86 .88% ,可应用于含重金属废水的处理 相似文献
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应用实测光谱估算千岛湖夏季叶绿素a浓度 总被引：4，自引：2，他引：2
依据2010年8月的实测数据构建了千岛湖水体夏季叶绿素a浓度的实测光谱数据估算模型,并进行了验证.利用ASD FieldSpec3野外光谱仪获取高光谱数据,计算水体离水辐亮度和遥感反射率.通过寻找反演水体叶绿素a浓度的高光谱敏感波段,采用单波段相关分析、波段比值、微分光谱、三波段模型、BP人工神经网络等多种算法进行比较分析,结果表明:叶绿素a浓度与单波段光谱反射率的相关性不大;596 nm和489 nm波长处反射率比值、545 nm处光谱一阶微分与叶绿素a浓度均呈较显著相关,估测模型决定系数R2分别为0.782、0.590,RMSE分别为0.89、1.98μg/L;三波段模型的反演结果优于传统的波段比值和一阶微分法,R2为0.838,RMSE为0.71μg/L;神经网络模型大大提高了叶绿素a浓度的反演精度,R2高达0.942,RMSE为0.63μg/L.本研究为今后在千岛湖水域的夏季相邻月份进行叶绿素a浓度大范围遥感反演研究奠定了基础. 相似文献
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秋季聚积蓝藻打捞对蓝藻生长及水质影响的原位实验 总被引：1，自引：0，他引：1
在巢湖西北半湖近岸设置3组小型围隔模拟秋季湖岸带蓝藻聚积,并用单片鳃式过滤器原位打捞蓝藻,研究秋季打捞对蓝藻生长的影响及其对营养盐、藻源性有机物的控制效应.初始围隔水体叶绿素a浓度为309.5±3.7μg/L,总氮和总磷浓度分别为3.32±0.14和0.30±0.04 mg/L.蓝藻衰亡分解释放的藻源性有机物为水体溶解性有机物的主要来源,荧光有机物以类蛋白物质为主.经过打捞,浮游植物生物量削减了41.7%,解除了蓝藻生长"密度制约",24 h细胞分裂频率及原位生长速率均增大,说明打捞在短期内增强蓝藻细胞生长活力,减缓藻源性有机物的释放.与秋季蓝藻衰亡趋势一致,实验周期内围隔中叶绿素a浓度逐渐降低,秋季打捞不会造成蓝藻水华再次暴发.打捞通过削减蓝藻生物量,使水体初级生产力、氮、磷、高锰酸盐指数得到显著控制;而且打捞还可以控制藻源性有机物的释放,使藻源性大分子有机物更易降解为小分子有机物.因此,在秋季对湖岸带聚积蓝藻进行物理打捞,不仅可以控制蓝藻生物量,还可以有效控制营养盐和有机物的释放,降低生态风险. 相似文献
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南京秦淮河叶绿素a空间分布及其与环境因子的关系 总被引：6，自引：2，他引：4
近年来,流动的河流开始陆续暴发藻类水华,河流的水华现象是继湖泊水华现象之后又一倍受关注的科学问题.秦淮河水体污染严重并于2010年8月和9月间暴发水华.采用2010年8月15日和9月8日的秦淮河野外调查数据,对秦淮河水体叶绿素a的空间分布特征及其与环境因子的相关关系进行研究.结果表明,从上游到下游,秦淮河水体叶绿素a含量的分布表现出一定的空间差异性,平均值为73.7μg/L,其中马木桥样点的叶绿素a含量最高(184.52μg/L);秦淮河水体氮磷比为26.86,水体叶绿素a浓度的对数与TP的对数呈正相关,与氮磷比的对数呈负相关,表明磷可能是秦淮河蓝藻生长的主要影响因子;河流水体叶绿素a浓度与pH和DO呈极显著正相关,与NO 2--N呈显著相关,而与NH 4+-N、NO 3--N和TN无显著相关. 相似文献
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添加固体碳源对垂直流人工湿地污水处理效果的影响 总被引：2，自引：0，他引：2
以垂直流人工湿地小试系统为研究对象,探讨了不同位置(表层、上层、中层和下层)添加固体碳源对系统氮、磷及CODCr去除效果的影响.结果表明:湿地下层硝态氮去除率低于中层,最适宜碳源添加位置为垂直流人工湿地中、下层.添加碳源系统中,碳源添加位置为表层的系统CODCr去除率最高,各系统出水CODCr浓度均低于进水,不引起系统出水中CODCr浓度的增加.添加碳源显著提高脱氮除磷效果,碳源添加位置为下层的系统TN去除率最高,碳源添加位置为表层的系统氨氧化作用明显,出水铵态氮浓度最高,各系统对亚硝态氮和硝态氮去除率差异不明显,但对硝态氮都表现出良好的去除效果.碳源添加位置为下层的系统硝化作用最完全,TP去除能力也显著优于其他各系统.添加碳源至垂直流人工湿地下层可以达到同步脱氮除磷的效果. 相似文献
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Taking Huanglong Ravine and Kangding, Sichuan, and Xiage, Zhongdian, Yunnan, as examples, the authors summarize the hydrogeochemical and carbon stable isotopic features of the geothermal CO2-water-carbonate rock system and analyze the CO2 sources of the system. It was found that the hydrogeochemical and carbon stable isotopic features of such a system are different from those of shallow CO2-water-carbonate rock system, which is strongly influenced by biosphere. The former has higher CO2 partial pressure, and is rich in heavy carbon stable isotope. In addition, such a geothermal system is also different from that developed in igneous rock. The water in the latter system lacks Ca2+, and thus, there are few tufa deposits on ground surface, but it is rich in light carbon stable isotope. Further analysis shows that CO2 of the geothermal CO2-water-carbonate rock system is a mixture of metamorphic CO2 and magmatic CO2. 相似文献
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《中国科学D辑(英文版)》2008,(Z1)
Kinetic experiments of gas generation for typical samples of marine gas precursors including low-maturity kerogen,residual kerogen and oil as well as dispersed liquid hydrocarbon(DLH)in source rocks were performed by closed system,and the evolution trends of molecular and isotopic compositions of natural gases from different precursors against the maturity(R0%)at laboratory conditions were analyzed.Several diagrams of gas origin were calibrated by using the experimental data.A diagram based on the ratio of normal and isomerous butane and pentane(i/nC4-i/nC5)was proposed and used to identify the origins of the typical marine natural gases in the Sichuan Basin and the Tarim Basin, China.And the maturities of natural gases were estimated by using the statistical relationships between the gaseous molecular carbon isotopic data and maturities(δ13C-R 0 %)with different origins.The results indicate that the molecular and isotopic compositions of simulated gases from different precursors are different from each other.For example,the dryness index of the oil-cracking gas is the lowest;the dryness indices of gases from DLH and kerogen in closed system are almost the same;and the dryness index of gases from residual kerogen is extremely high,indicating that the kerogen gases are very dry;the contents of non-hydrocarbon gases in kerogen-cracking gases are far higher than those in oil-cracking and DLH-cracking gases.The molecular carbon isotopes of oil-cracking gases are the lightest,those of kerogen in closed system and GLH-cracking gases are the second lightest,and those of cracking gases from residual kerogen are the heaviest.The calibration results indicate that the diagrams of ln(C1/C2)-ln(C2/C3)andδ13C2-δ13C3-ln(C2/C3)can discriminate primary and secondary cracking gases,but cannot be used to identify gas origin sources,while the diagram of i/nC4-i/nC5 can differentiate the gases from different precursors.The application results of these diagrams show that gas mixtures extensively exist in China,which involved the gases from multiple precursors and those from different maturity stages.For example,marine gases in the Sichuan Basin involve the mixture of oil-cracking gases and high-over-maturated kerogen gases,while those in the Tarim Basin involve not only the mixture of gases from multiple precursors,but also those from different maturity gases and post-reservoir alternations such as oxidized degradation and gas intrusion processes. 相似文献
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Transport and delivery of suspended solids, nitrogen and phosphorus from various sources to freshwaters in the UK 总被引：8，自引：0，他引：8
Sources of nutrients and suspended solids (SS) are diverse in origin and dynamic with respect to their transport pathways and delivery to surface and groundwaters. Three broad source groups can be identified based on their hydrological and compositional characteristics. Consented discharges from point sources are generally continuous and highly concentrated relative to receiving waters while non-point (diffuse) sources are generally more dilute with SS and phosphorus in particular tending to have a high transport dependence upon hydrological (storm) events. A third group with properties that are intermediate include overflows from septic systems, seepages from farmyards or road/track runoff. This grouping automatically introduces a strong temporal and spatial variability in the relative contribution individual group sources make to the total quantity of nutrients and SS delivered to freshwaters. This spatial or temporal variability in either effluent volumes or composition is generally not considered when predicting impacts, which can be highly significant in headwater streams. Complete compositional data for individual sources (especially the intermediary group) are also often lacking when apportioning source contributions in catchments, being simply lumped into average export coefficients. Better definition of intermediary sources would help to more accurately define the relative contribution of different sources and enable cost-effective river basin management planning as required under the Water Framework Directive. 相似文献
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In the densely populated Wuppertal-Düsseldorf area the atmospheric concentrations of CCl2F2 and CCl3F near ground level were measured by ECGC and GC-MS method. Special systems for sampling and calibrating were developed. As mean concentration averages over the time period from December 1976 to May 1977 a value of 650±356 pptv was determined for CCl2F2 (F12) and of 490±279 pptv for CCl3F (F 11). 相似文献
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More than a thousand acoustic-emission (AE) hypocenters were determined in a cylindrical andesite specimen under two-stage uniaxial creep at stresses of 204 and 214 MPa. Strains were monitored for 6 peripheral points at the middle part of the cylindrical specimen's wall. The strain data indicate gradual increase of nonuniform deformation during steady creep and strong intensification of the nonuniformity during acceleration creep and, therefore, biased stress distribution within the specimen. The correlation between dilatant strain and AE hypocenters was investigated for whether or not tensile cracks emit AE. The region with high AE activity shows only a small dilatant strain. This negative correlation between AE and the dilatant region may eliminate tensile cracks as possible AE sources. A composite focal-mechanism solution of local AE events, covering a wide solid angle of the focal hemisphere, indicates that shear fractures emit AE waves. The direction of the compressional axis in this solution shows a significant deviation from that inferred from the applied external force, suggesting that the local stress field is governed by preexisting weak zones that are, presumably, produced by tensile cracks within the specimen. AE hypocenters tended to form clusters during steady creep under the constant compressional stress. During acceleration creep caused by a small step increase of the external stress, the preceding clusters disappeared while a new cluster appeared in an incipient fault plane. This suggests that changes in seismicity pattern such as migrations or quiescences of swarm—important clues for earthquake predictions—may be caused by an instantaneous change in the tectonic-stress levels. 相似文献
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The seismic wave field is considerably influenced by local structures close to the source and to the receiver. This applies to sources and receivers situated close to localized inhomogeneities, to structural interfaces, to the earth's surface, etc. In this paper we concentrate our attention mainly to the ray-theoretical radiation patterns of point sources situated close to the structural interfaces and to the earth's surface. In numerical modeling of high-frequency seismic wave fields by the ray method, the interaction of the source with the earth's surface has not usually been taken into account.The proposed procedure of the computation of the radiation patterns of point sources situated directly on structural interfaces and on the earth's surface is based on the zero-order approximation of the ray method, assuming that the length of the ray between the source and the receiver is long. The derived equations are extended to point sources located close to structural interface, to the earth's surface and to thin transition layers using the hybrid ray-reflectivity method, seeervený (1989). The thin layer need not be homogeneous; it may include an arbitrary inner layering (transition layers, laminas, etc.) The only requirement is for the layer to be thin. Roughly speaking, we require its thickness to be less than one quarter of the prevailing wavelength. The hybrid ray-reflectivity method describes well even certain non-ray effects (tunneling.S
* waves, etc.). Explicit analytical expressions for radiation patterns for all above listed point sources are found. These expression have a local character and may be easily implemented into computer codes designed for the routine computation of ray amplitudes and synthetic ray seismograms in 2-D and 3-D, laterally varying isotropic layered and block structures by the ray method.Numerical examples of radiation patterns ofP andS waves of point sources situated close to the earth's surface and to a thin low-velocity surface layer are presented and discussed. The explosive point source (center of dilatation) and the vertical and horizontal single force point sources are considered. It has been ascertained that the radiation patterns of point sources depend drastically on the depth of the source below the surface even if the depths vary within one quarter of the prevailing wavelength. 相似文献
20.
Polyhydroxyalkanoates (PHAs), environmental friendly polyesters, can be produced by a wide range of microorganisms in nutrient‐limiting conditions. In the present study a new bacterial strain, able to produce high quantities of poly‐3‐hydroxybutyrate (PHB), is isolated from contaminated soil. The isolate is characterized and identified as Bacillus cereus Y23, based on morphological, physiological characteristics, and 16S ribosomal RNA gene sequences. PHB production is evaluated using different carbon and nitrogen sources and nutritional and culture conditions are optimized. The highest PHB production of 5.12 g L?1 was obtained in growth medium containing fructose and ammonium sulfate at a C/N ratio of 30, 0.3% yeast extract, 4.5 g L?1 phosphate solution, pH 7, inoculum size of 0.3%, and agitation rate of 150 rpm after 48 h of incubation at 32 °C. The PHB production decrement can be attributed to the intracellular utilization of the polymer during the stationary phase of the cellular growth and to the sporulation process. The presence of the main functional groups of PHB polymer is verified by Fourier‐transform infrared analysis. At optimum conditions, B. cereus Y23 turned out to be a good candidate for industrial production of PHB since it accumulates up to 67.9% of its dry weight. 相似文献