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1.
A method is described for the specific hydrolysis of amino acids from humic substances by using the proteolytic enzyme “Pronase E”. The amino acids are analyzed by a fluorescence detector after precolumn derivatization and HPLC-separation. The pattern and the concentration of amino acids after the enzymatic hydrolysis are set up for humic substances of different origin. Due to the results the enzymatic hydrolysis is suitable for the characterization of humic substances with a nitrogen content higher than 3%. As shown in a kinetic study of the hydrolysis of a fulvic acid the influence of humic substances on the enzyme activity increases in case of lower nitrogen content. Due to the specificity of the proteolytic enzyme the operationally defined method is limited as shown by a protein of a known amino acid content. The amino acids leucine, valine, tyrosine and histidine are released to the highest extent. The determined total extent for the hydrolysis of Insulin B is 26.34% and for Casein 38.37%. By comparison of the enzymatic hydrolysis with the classic acid hydrolysis the advantages of the biochemical method are the simple experimental handling and the mild reaction conditions. Amino acids which are very unstable like glutamine and asparagine can only be analyzed by the enzymatic hydrolysis. 相似文献
2.
Nitrogen contents and isotopic ratios have been determined for three clasts from the enstatite chondrite Abee by stepwise heating. The clasts possess a wide range in nitrogen content, ranging from 254 to 850 ppm, whereas the nitrogen isotopic ratios are nearly identical atδ15N= ?29.2±0.6‰. A refractory inorganic nitrogen-bearing phase contains about 90% of the nitrogen which is released at temperatures of 1000°C and above. The stepwise heating experiments suggest the possible existence of two other distinct nitrogen components, released at low (770°C) and high (1500°C) temperatures. 相似文献
3.
María del Carmen Scapini Víctor Hugo Conzonno Vilma Teresa Balzaretti Alicia Fernández Cirelli 《Aquatic Sciences - Research Across Boundaries》2010,72(1):1-12
Structural aspects of humic substances (HSs) in marine waters of Engaño Bay (Atlantic Ocean) and water from the Chubut River (Patagonia, Argentina) were compared. The HSs were isolated, purified, and analyzed using a multiple-method approach for structural characterization: elemental analysis, nuclear magnetic resonance (13C and 1H NMR), infrared spectroscopy (FTIR), UV-visible absorption and fluorescence. Similarities between the marine and freshwater components were evaluated on the basis of N and O contents, H/C and C/N atomic ratios, infrared bands from nitrogen-containing and carboxylic groups, percentage of functional groups obtained from NMR spectra, spectral slope coefficient of absorption spectra, absorbance ratios at 250 and 365 nm (E 2/E 3), aromaticity, excitation–emission matrices and fluorescence quantum yield. Both the Engaño Bay and Chubut River waters have HSs that are mainly composed of fulvic acids (FAs); although they are very similar, they may be distinguished from one another. Similarities include the predominance of aliphatic carbon content (low aromatic content); however, the water bodies differed in their content of proteins and carboxylic acids. The Engaño Bay FAs have mainly an aquatic origin; although the Chubut River FAs suggest a similar aquagenic origin, there is also evidence of contributions from pedogenic FAs. 相似文献
4.
A characterization method for AOX in surface water samples was developed and tested. The method involves fractionation using a hydrophobic C18 resin and a weak anionic exchange resin and allows the fractionation of the AOX pool of surface water samples into four fractions: (1) hydrophilic acidic, (2) hydrophilic non‐acidic, (3) hydrophobic acidic, and (4) hydrophobic non‐acidic. The adsorption analysis was verified with AOX‐relevant model compounds and was applied to characterize the AOX pool of a stream sample from the Moskva river (Russia). In addition to the fractionation analysis, size‐exclusion chromatography was used to characterize the AOX pool of the sample studied. Hydrophilic acids made up the major fraction of the AOX pool (55 %). Among this fraction chlorinated high‐molecular acids (humic substances) made up the main fraction (35 %). 相似文献
5.
A phenoloxidase-producing fungus – Cladosporium cladosporioides – was isolated from water of a bog lake. At high concentrations of carbon (333 mM) and nitrogen (12 mM), the fungus demonstrated the formation of a laccase and a high ligninolytic activity. After addition of riverine or groundwater humic substances into culture media (ca. 1 mg mL–1), the fungus showed the ability of degrading (utilizing) of about 60% of these substances. However, the exact quantification of the degree of degradation was difficult because of adsorption of humic matter on fungal mycelium. Reisolated humic substances were lower in aromatic and higher in aliphatic structures. A crude enzyme from the C. cladosporioides culture showed only low activity in decolorizing humic substances, whereas decolorization up to 50% was observed when using a laccase preparation from Polyporus versicolor, and especially in the presence of a redox mediator. 相似文献
6.
K. G. Heumann G. Rdlinger M. Erbes I. Heiber U. Obst Z. Filip H. Claus 《洁净——土壤、空气、水》2000,28(4):193-201
The distribution of halogens in various fractions of humic substances (HS), separated by their molecular weight, was found to be different for the different halogens. This was demonstrated for chlorine, bromine, and iodine in sewage and brown water samples by applying inductively coupled plasma mass spectrometry coupled with size‐exclusion chromatography. Quantification of the different fractions of iodinated humic substances was obtained by quadrupole mass spectrometry in connection with the isotope dilution technique using an 129I‐enriched spike solution. Quantitative analysis was not possible for the corresponding chlorine and bromine species because of spectrometric interferences in the quadrupole instrument. The ageing of HS/halogen species was followed with respect to possible transformations of these species in a ground and sewage water sample up to eight weeks. Even if a distinct structural variation of the humic substance was observed with time by measuring the UV absorption, chlorine remained in the same molecular weight fraction and only a small change was found for the HS/bromine species after eight weeks. In contrast to these findings a substantial transformation of HS/iodine compounds took place, which demonstrated that the transfer probability of halogens from one to another HS fraction is increased with decreasing strength of the halogen bond to carbon. By comparing the results of an original sewage water sample with a filtered one and with another one which was enriched by microorganisms cultivated from the same original sample, a strong microbiological influence on the transformation of HS/iodine species was found. A quantitative balance of the corresponding HS/iodine fractions was calculated for an ageing period of eight weeks showing that iodine was preferably transferred to newly formed UV active HS substances of high molecular weight. In total, no iodine was released from the humic substances. 相似文献
7.
蓝藻水华暴发和衰亡对太湖有色可溶性有机物的影响 总被引：1，自引：1，他引：0
利用平行因子分析(PARAFAC)技术对藻华暴发季节太湖梅梁湾和开敞区水样中有色可溶性有机物(CDOM)的三维荧光光谱进行分析,获得代表类酪氨酸、类色氨酸和类腐殖酸等3种荧光组分,这3个组分的荧光得分值均与叶绿素a浓度呈极显著正相关,其中类腐殖酸物质荧光得分值占总分值的比例也与叶绿素a浓度极显著正相关,由此可以推测,蓝藻水华可能是太湖CDOM的一个重要来源,并极大地影响了湖泊CDOM的组成结构.为进一步探索蓝藻水华对湖泊CDOM的影响,于太湖梅梁湾湖岸进行藻华暴发和衰亡模拟试验.PARAFAC结果显示,模拟试验水样CDOM的三维荧光图谱同样含有3个组分,其中类酪氨酸和类色氨酸荧光峰位置与湖泊水样基本相同,而类腐殖酸物质荧光峰则有一定程度的蓝移,表明培养试验中有新的内源类腐殖酸物质产生.利用PARAFAC模型中的得分值计算后发现,初始叶绿素a浓度越高,类腐殖酸物质所占比例越大,而实验结束时添加了高浓度和中等浓度藻的2个处理中类腐殖酸物质对荧光的贡献率也有显著提高.因此,藻华的长期暴发可以显著改变湖泊CDOM的组成,导致类腐殖酸物质比例提高. 相似文献
8.
Humic material isolated from six Himalayan lakes was investigated for its elemental composition, exchange behaviour and spectrophotometric properties. The carbon content was 36.8… 56.6 %. The C: H ratio was lower than for soil humic acids. The nitrogen content was 3.8… 5.8 % and was higher than reported for soil humic acids. Zinc and copper were higher at the sites with high pollution load. On a strong cation exchanger copper showed the highest stability and calcium was least stable. The Δ log K values suggested that comparatively deep water sediments show lower degrees of humification. 相似文献
9.
After an explanation of the complex composition of the system of humic substances, some gravel deposits are described from whose open-pit boundaries there were taken water samples, their content of components of humic matter having been determined. Since spectroscopic analysis techniques yield spectra which are difficult to analyse and have a relatively low detection sensitivity, an indirect polarographic method was applied by which water-soluble humic substances can be determined very selectively down to a lower limit of 5 μg/1. Thus, enrichment in water or in adsorbing columns is not required. From the results found there are derived several statements concerning the occurrence of humic substances and fulvic acids in waters and conclusions about the possible influence on the hardening of concrete are given. 相似文献
10.
Sixteen samples of fulvic acids and XAD‐4 fractions of riverine, estuarine, coastal, and open ocean origin have been studied by emission and synchronous molecular fluorescence spectroscopy. Certain features of the molecular fluorescence are related to the nature, the content, and the origin of those aquatic humic substances (HS). Riverine HS appear several times richer in fluorophores than marine HS, which can be well observed by emission fluorescence spectroscopy. Synchronous‐scan spectra of fulvic acids and of XAD‐4 fractions from the aquatic environments studied, emphasized the quality differences of their fluorophores. These features are useful as tracers of humic substances related with their natural environment source or even with their anthropogenic origin. 相似文献
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12.
Recently emerged indications imply that particular concentrations and qualities of dissolved organic carbon (DOC) have the potential to reduce species diversity in zooplankton. In this contribution, we tested the impact of a major component of DOC, the dissolved humic substances (HSs), on two life history traits of a clone of Daphnia magna, namely lifespan and reproduction. We chose reproduction as a variable since it guarantees the persistence of the population in its habitat, and lifespan was selected as the most integrative individual variable. We found that lifespan was extended by exposure to HSs, but lifetime reproduction was clearly reduced. On the other hand, the mineral and biochemical composition of food also significantly modulated these life history traits. By feeding coccal green algae that contained linolenic acid, arginine (Arg), and histidine (His), but that lacked polyunsaturated fatty acids (PUFAs) with C-back bones >18, we examined how short-chained PUFAs, either alone or in concert with Arg and His, are able to modulate life history traits. Our expectation was that the additional challenge by HSs would shift the energy allocation in favor of reproduction. We found that the carbon and amino acid content may account for longevity, even when challenged by HSs. Reproduction appeared to be triggered by the C:P and N:P ratios of the food, particularly given the rather low P-content, which was within the range of severe growth limitation. Consequently, exposure to HSs reduced offspring numbers. Resting eggs without males occurred only in Desmodesmus armatus fed Daphnia that were simultaneously exposed to HSs. Under these conditions, the total amino acid (AA) content seemed to trigger reproduction. In summary, exposure to HSs appeared to have the potential to change the competitive ability of zooplankters by modulating the energy allocation between body maintenance and reproduction. 相似文献
13.
Microbial biodegradation of organic substances takes place during drinking water treatment, but also in the distribution net, if the drinking water still contains biodegradable organic substances. This phenomenon is called regrowth. The regrowth potential of a drinking water is high, when for instance ozonation is used as the last step of treatment. It was proved before, that ozonation increases the biodegradability of humic substances, which are the main fraction of organic carbon in drinking water. In this work the objective was to check, if chlorination has a similar effect on humic substances. Using the method developed by Werner of measuring the regrowth potential of a water it could be shown by dilution series with chlorinated and unchlorinated humic substances that the substrate quality of these organics is increased by chlorination. The better substrate quality is seen in the chlorinated solutions, which might contain also low molecular weight organic compounds, but also in the fulvic acid fraction after XAD-enrichment, which removes most of the low molecular weight organic compounds. The chlorination creates in the fulvic acid solutions a shift of molecular size to smaller molecules and higher polar substances, which might be the reasons for the better biodegradability. From these results it can be concluded, that chlorination produces substances, which are more easily biodegradable. But this will not produce regrowth problems as long as there is free chlorine present as a disinfectant. In contrast, when the chlorine demand is very high and no free chlorine is left, this might produce high colony counts in the distribution net. 相似文献
14.
为探究富营养化湖泊中自生源可溶有机质(DOM)在泥水界面的吸附行为,以马来眼子菜(Potamogeton malaianus)和铜绿微囊藻(Microcystis aeruginosa)释放的DOM为代表,考察针铁矿对草、藻源DOM中不同组分的吸附特征.三维荧光平行因子分析表明类富里酸组分C1和类胡敏酸组分C4的含量很低,而类蛋白物质(类酪氨酸组分C2和类色氨酸组分C3)分别占草、藻源DOM荧光组成的70%和93%. 2种DOM均可被针铁矿吸附,吸附过程符合伪一级动力学.通过等温线拟合发现针铁矿吸附藻源DOM的非线性更强,饱和吸附量(23.77 mg/g)高于草源DOM(19.10 mg/g).特别地,类蛋白组分呈现非线性吸附,而类腐殖物质的吸附近似线性,且针铁矿对于DOM各荧光组分的吸附量顺序为:C3C2 C4 C1,此非均质吸附特征与DOM组分的初始含量、分子大小、芳香性及有效吸附位点有关.红外光谱证实氨基、羧基和羟基是吸附过程中的重要官能团.因此,草、藻源DOM显著改变了针铁矿表面的有机物质组成,影响湖泊沉积物的生物地球化学行为. 相似文献
15.
M. I. Dinu 《Water Resources》2010,37(1):65-69
Complex formation reactions with the interaction of fulvic and humic acids with zinc and iron ions in a model aqueous system.
The complexing ability of humic acids was experimentally proved to be much higher than that of fulvic acids. The complexing
ability of fulvic acids is found to decrease over time. The dependence of the complexing ability of heavy metals on the types
of ion and humic substances and the proportions of components in the solution is examined. The obtained experimental results
on the occurrence forms of heavy metals were compared with their theoretical estimates calculated for natural water bodies. 相似文献
16.
17.
John D. Coates Romy Chakraborty Susan M. O&#x;Connor Claudia Schmidt Jürgen Thieme 《洁净——土壤、空气、水》2001,28(7):420-427
As part of a study on microbial redox alteration of humic substances we investigated the potential effect of this metabolism on the fate of heavy metals and hydrocarbons as a result of conformational alteration of the humic molecular structure due to microbial reduction. Our studies indicate that the microbial reduction of humic acids (HA) results in significant morphological and geochemical alterations. X‐ray microscopy analysis indicate that the conformational structure of the humic colloids is altered as a result of the redox change. In the reduced state, the HA appeared as small dense particles, on reoxidation, large loose aggregates were formed. In addition, spectrofluorometric studies indicated that the binding capacity of the HA for naphthalene was decreased by 10% when the HA was reduced. Similarly, the reduced HA yielded higher surface tension values at all concentrations tested which is indicative of a more hydrophilic and less hydrophobic solute. On reoxidation, the surface tension values reverted back to values similar to those obtained for the untreated oxidized HA. These data indicate that the hydrophobicity of the HA is altered on biological reduction of the HA and that this alteration is reversible. In contrast the reduced HA demonstrated a 15% higher affinity for heavy metals such as divalent cobalt than the oxidized HA. In addition to increasing the binding capacity of HA for heavy metals, the reduction of the HA also decreased the bioavailability and toxicity of bound heavy metals such as chromium. When incubated in the presence of Cr(III) and HA, cells of Escherichia coli grew much more rapidly in the presence of the reduced HA suggesting that the higher metal binding capacity of the reduced humic substances resulted in a removal of the Cr(III) from solution and hence reduced its bioavailability and toxicity. These studies demonstrate that HA redox state and reduction of humic acids by microorganisms can have a significant effect on the molecular morphology and binding constants of HA for heavy metals and hydrocarbons and also directly affects the bioavailability of these compounds in the environment. 相似文献
18.
M. Feuillade J. Feuillade V. Fiala 《Aquatic Sciences - Research Across Boundaries》1990,52(4):345-359
The effect of light intensity on the release of dissolved organic carbon during photosynthesis on NaH14CO3 was investigated using the phytoplanktonic CyanobacteriumOscillatoria rubescens. The released products were fractionated by molecular size and chemical identifications attempted using combined thin-layer electrophoresis and chromatography, and high pressure liquid chromatography.Within the range of irradiances tested (from 6 to 60 µmole m–2 sec–1), though the upper one inhibited photosynthesis ofO. rubescens, light had little effect on the quantity and composition of the excreted products. The released carbon was always lower than 3% of the incorporated carbon, and mainly composed (62 to 86%) by small molecular weight compounds. The prevailing identified compounds were amino acids which represented more than 20% of the excreted carbon. Among organic acids, glycolic acid accounted for less than 2% of the recovered radioactivity. Glucose was the only identified sugar.Abbreviations EOC
excreted organic carbon
- DOC
dissolved organic carbon
- PER
percent extracellular release
- LMW
low molecular weight
- HMW
high molecular weight
- AA
amino acids
- µmoles
m–2 sec–1 = µEinsteins m–2 sec–1 相似文献
19.
A combination of sephadex gel-chromatography and polyacrylamide gel electrophoresis was used to obtain stable electrophoretic
fractions with different molecular sizes from a preparation of humic substances isolated from river water by reverse osmosis.
The photochemical activity of humic substances and their fractions was determined. For the first time it is shown that low-molecular
fractions of aquatic humic substances, when irradiated by polychromatic light, can intensely fluoresce and initiate the formation
of singlet O2, effectively destroying model compound furfural. The photochemical activity and intensity of fluorescence of high-molecular
fractions is insignificant. The obtained data will help to extend the current knowledge in the field of natural water hydrochemistry
about the ways of its natural self-purification from anthropogenic pollutants. 相似文献
20.
《Acta Geochimica》2016,(1)
Characteristics and distributions of humic acid(HA) and soil organic matter(SOM) in a yellow soil profile and a limestone soil profile of the southwest China Karst area were systematically investigated to reveal their evolutions in different soils of the study area. The results showed that characteristics and distribution of SOM along the two soil profiles were notably different. Total organic carbon(TOC) contents of soil samples decreased just slightly along the limestone soil profile but sharply along the yellow soil profile. TOCs of the limestone soils were significantly higher than those of the corresponding yellow soils, and C/N ratios of SOMs showed a similar variation trend to that of TOCs, indicating that SOM can be better conserved in the limestone soil than in the yellow soil. The soil humic acids were exhaustively extracted and further fractionated according to their apparent molecular weights using ultrafiltration techniques to explore underlying conservation mechanisms. The result showed that C/N ratios of HAs from different limestone soil layers were relatively stable and that large molecular HA fractions predominated the bulk HA of the top soil, indicating that HA in the limestone profile was protected while bio and chemical degradations were retarded. Combined with organic elements contents and mineral contents of two soils, weconcluded that high calcium contents in limestone soils may play a key role in SOM conservation by forming complexation compounds with HAs or/and enclosing SOMs with hypergene CaCO_3 precipitation. 相似文献