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1.
Neomysis awatschensis was collected on the west coast of Jiaozhou Bay,Qingdao in 1992 and acclimated in the laboratory conditions.The acute toxicity method using 4 ~6 day juvenile mysid of this species is studied.The species is compared with M.bahia in taxonomy,geographic distribution,morphology,as well as the sensitivity to reference toxins,Based on the results,we recommend N.awatschensis to be a standard marine organism for toxicity test in China and the method to be applied in toxicity evaluation of drilling fluids.In this paper,we report the results of the above studies and the application of the method in the evaluation of several drilling fluid products.  相似文献   

2.
Microalgae are photosynthetic microorganisms that function as primary producers in aquatic ecosystems. Some species of microalgae undergo rapid growth and cause harmful blooms in marine ecosystems. Heterocapsa triquetra is one of the most common bloom-forming species in estuarine and coastal waters worldwide. Although this species does not produce toxins, unlike some other Heterocapsa species, the high density of its blooms can cause significant ecological damage. We developed a H. triquetra species-specific nuclease protection assay sandwich hybridization(NPA-SH) probe that targets the large subunit of ribosomal RNA(LSU r RNA). We tested probe specificity and sensitivity with five other dinoflagellates that also cause red tides. Our assay detected H.triquetra at a concentration of 1.5×104 cells/m L, more sensitive than required for a red-tide guidance warning by the Korea Ministry of Oceans and Fisheries in 2015(3.0×104 cells/m L). We also used the NPA-SH assay to monitor H. triquetra in the Tongyeong region of the southern sea area of Korea during 2014. This method could detect H.triquetra cells within 3 h. Our assay is useful for monitoring H. triquetra under field conditions.  相似文献   

3.
The material discussed in this report was collected at 23 stations in the north of the continental shelf of the East China Sea from October 4 to November 5, 1977 with a Juday phytoplankton net (diameter 37 cm, No. 20 gauze) which was towed from the bottom to the surface of the sea. The Results anlayzed show that the species composition and distributon of dinoflagellates in this region bear a close realtionship to the water mass and the sea currents. Based on the temperature ranges in which different species of dinoflagellates flourish, they may be divided into three groups: cold-water species, warm-temperate species and warm-water species. The number of the last group is more than 70% of the total.  相似文献   

4.
Epizoic diatoms on marine copepods are common in nature and may have a special ecological relationship with their hosts.However,this special ecological group is not well known,and it has only rarely been studied in the China seas.To address this knowledge gap,the species diversity and classification of epizoic diatoms on planktonic copepods were studied with samples collected from the East China Sea.In the present study,a marine araphid diatom genus Protoraphis and its type species,Pr.hustedtiana,were observed and identified by light and electron microscopy,thus representing the first record of this genus and its type species in China.This genus is characterized by a median sternum strongly bent to opposite sides and terminate in two transapical grooves at the valve ends.Protoraphis hustedtiana was found to be epizoic on the posterior body appendages and segments of the marine calanoid copepod Candacia bradyi.An internal view shows a complex,ear-shaped process that is close to the apical slit field.The ecological habitats and geographical distributions of Protoraphis were also discussed,and,together with complementary morphological studies,our results have increased the number of records for marine epizoic diatoms to three genera with three species in China,including Pseudohimantidium and Pseudofalcula.  相似文献   

5.
Intracytoplasmic infection with a prokaryotic mycoplasma-like organism (MLO) were characterized in the scallop Argopecten irradians, and the first report of such infection in this scallop was represented. Ultrastructurally these microorganisms are usually pleomorphic and variable in morphology and size, and appear in several cell types such as small spherical body, rod-shaped bodies and longer filament-shaped body. They lack a cell wall. These observations revealed that these types of the prokaryote showed some characteristics of a mycoplasma-like organism (MLO). MLO reproduced in two ways: binary fission and budding. The results of isolation and purification showed that a large number of MLOs existed in the tissues of diseased scallops. The results of experimental infection revealed that the MLO is pathogenic to the scallop Argopecten irradians.  相似文献   

6.
Uplift response of symmetrical anchor plates with and without grid fixed reinforced (GFR) reinforcement was evaluated in model tests and numerical simulations by Plaxis. Many variations of reinforcement layers were used to reinforce the sandy soil over symmetrical anchor plates. In the current research, different factors such as relative density of sand, embedment ratios, and various GFR parameters including size, number of layers, and the proximity of the layer to the symmetrical anchor plate were investigated in a scale model. The failure mechanism and the associated rupture surface were observed and evaluated. GFR, a tied up system made of fiber reinforcement polymer (FRP) strips and end balls, was connected to the geosynthetic material and anchored into the soil. Test results showed that using GFR reinforcement significantly improved the uplift capacity of anchor plates. It was found that the inclusion of one layer of GFR, which rested directly on the top of the anchor plate, was more effective in enhancing the anchor capacity itself than other methods. It was found that by including GFR the uplift response was improved by 29%. Multi layers of GFR proved more effective in enhancing the uplift capacity than a single GFR reinforcement. This is due to the additional anchorage provided by the GFR at each level of reinforcement. In general, the results show that the uplift capacity of symmetrical anchor plates in loose and dense sand can be significantly increased by the inclusion of GFR. It was also observed that the inclusion of GFR reduced the requirement for a large L/D ratio to achieve the required uplift capacity. The laboratory and numerical analysis results are found to be in agreement in terms of breakout factor and failure mechanism pattern.  相似文献   

7.
Ben Belgacem  I.  Cheikh  L.  Barhoumi  E. M.  Khan  W.  Ben Salem  W. 《中国海洋工程》2019,33(1):114-126
In this paper, we present a numerical simulation of a water jet impacting a new aeronautical material Ti555-03 plate.The Computational Fluid Dynamics(CFD) behavior of the jet is investigated using a FV(Finite Volume) method.The Fluid–Structure Interaction(FSI) is studied using a coupled SPH(Smoothed Particle Hydrodynamics)-FE(Finite Element) method. The jets hit the metal sheet with an initial velocity 500 m/s. Two configurations which differ from each other by the position(angle of inclination) of the plate relatively to the axis of revolution of the jet inlet are investigated in this study. The objective of this study is to predict the impact of the fluid produced at high pressure and high speed especially at the first moment of impact. Numerical simulations are carried out under ABAQUS. We have shown in this study that the inclination of the titanium alloy plate by 45° stimulates the phenomenon of recirculation of water. This affects the velocity profile, turbulence and boundary layers in the impact zone. The stagnation zone and the phenomenon of water recirculation are strongly influenced by the slope of the plate which gives a pressure gradient and displacement very important between the two configurations. Fluctuations of physical variables(displacement and pressure) prove the need for a noise and vibratory study. These predictions will subsequently be used for the modeling of the problem of an orthogonal cut in a high-speed machining process assisted by high-pressure water jet.  相似文献   

8.
The uptake of zinc and cadmium by a species of marine diatom Thalassirosira weissflogii and the nutrient variation (phosphorus and silicon) in the culture medium were estimated when exposed to zinc and cadmium stress under controlled laboratory conditions.It was found that low cadmium addition could stimulate the culture to grow with a relatively high rate and exert its toxicity at the elevated concentration.The cellular uptake of zinc and cadmium by the diatom varied in the ranges of 1.21~3.75 and 0.060~0.629 fmol per cell respectively in the whole cultivation period.The mean cellular phosphorus and silicon drawdowns were constant with 0.26~0.42 and 0.30~0.46 pmol per cell respectively in those healthy cultures.It is illustrated that the algal cells can adjust some physiological mechanisms to decrease metal accumulation and keep metal homeostasis in the organism.The correlation analysis results further indicate that the cellular cadmium uptake might be closely related with the cellular phosphorus, silicon drawdown and the growth rate of diatom (P<0.05, 0.001, 0.01); and the cultures grow better in good conditions, the lower amount of phosphorus, silicon materials are consumed by a single cell to keep a high efficiency of utilization.  相似文献   

9.
The ecology of the family Pinnidae was studied by sampling three pinnid species from 36 sampling sites across four different microhabitats in the Gulf of Thailand. The species spatial distributions were mostly uniform, with some populations having random distributions. Species abundances differed between sandy and coral habitats according to non-metric multi-dimension scaling analyses. Although the Gulf of Thailand is a relatively small geographic area, habitats are varied enough to provide variable shell densities. Small islands are important distribution areas, and coral reefs provide both direct and indirect shelter which support high abundances, densities and increased shell size. The highest density was recorded in sand beds within coral reefs. Low density and small shell size in sand beaches might be related to high mortality in shallow water or to adaptations for survival in shallow waters. A clear correlation between sediment composition and species abundance was found in Pinna atropurpurea; abundance increased with the sand content of the sediment. For P. deltodes, abundance increased as the rock fraction of the sediment increased. These results suggest that adaptations in Pinnidae, such as shell size, shell morphology, and the exposure of the shell above the sediment-water interface, are responses for survival in different habitats.  相似文献   

10.
Euniphysa, originally a genus of the family Eunicidae, was erected by Wesenberg-Lund in 1949, and for a longtime there has been only one species in this genus. The present report is based on our samples collected from the South China Sea, and we consider that the differences between Euniphysa and Eunice are very significant: Five occipital tentacles distributed unequidistantly; the jaw consists of 6 pairs of maxillary plates; the body may be divided into anterior region and posterior region, etc. Therefore we suggest that Euniphysa should be separated from Eunicidae, and become a new family Euniphysi-dae. At present this family includes 3 genera, namely, Euniphysa , Paraeuniphysa and 1 new genira Heterophysa; Totally there are 7 species, including 3 new species and 1 new combination species.  相似文献   

11.
Microalgae are photosynthetic microorganisms that function as primary producers in aquatic ecosystems. Some species of microalgae undergo rapid growth and cause harmful blooms in marine ecosystems. Heterocapsa triquetra is one of the most common bloom-forming species in estuarine and coastal waters worldwide. Although this species does not produce toxins, unlike some other Heterocapsa species, the high density of its blooms can cause significant ecological damage. We developed a H. triquetra species-specific nuclease protection assay sandwich hybridization (NPA-SH) probe that targets the large subunit of ribosomal RNA (LSU rRNA). We tested probe specificity and sensitivity with five other dinoflagellates that also cause red tides. Our assay detected H. triquetra at a concentration of 1.5×104 cells/mL, more sensitive than required for a red-tide guidance warning by the Korea Ministry of Oceans and Fisheries in 2015 (3.0×104 cells/mL). We also used the NPA-SH assay to monitor H. triquetra in the Tongyeong region of the southern sea area of Korea during 2014. This method could detect H. triquetra cells within 3 h. Our assay is useful for monitoring H. triquetra under field conditions.  相似文献   

12.
车轮虫是一类主要寄生于贝类和鱼类体表的缘毛类纤毛虫原生动物。本文对分别寄生于海洋贝类--虾夷扇贝Mizuhopecten yessoensis,半咸水鱼类--鲻鱼Mugil cephalus和双色鳗鲡Anguilla bicolor bicolor,以及淡水鱼类--光唇鱼Acrossocheilus fasciatus鳃表和体表的虾夷扇贝车轮虫Trichodina pectenis,亚卓车轮虫T.jadranica,急尖车轮虫T.acuta和喙状车轮虫T.rostrata,进行了详细的分类学研究。本文第一次报道了虾夷扇贝车轮虫的活体和蛋白银染色后特征,该虫体也是国内新记录种。其它三个车轮虫物种感染的鱼类,均属新纪录宿主。同时,也报道了这四种车轮虫对宿主的感染率和感染强度。  相似文献   

13.
中蚓虫是小头虫科较为常见的属,中国海区的样品过去常被鉴定为加洲中蚓虫Mediomastus californiensis Hartman。本研究中,来自东南沿海四个不同海区的118条中蚓虫样品被鉴定为一个新的种类,在此给出具体描述。这个新种具有如下特征:(1)第1至9刚节表皮双环型,刚毛束位于体节中间位置,而后的刚节多环型,刚毛束位于体节后半部;(2)后腹区具有疣足突起;(3)前4个胸刚节仅具双羽型翅毛状刚毛,其它体节仅具巾钩刚毛;(4)胸区巾钩刚毛的柄长且直,巾长为宽的3至5倍;(5)腹区巾钩刚毛不同于胸区,较短且具收缩部;(6)甲基绿染色模式独特。这个研究表明了中国海区的中蚓虫可能被误鉴定为加洲中蚓虫,而这个新种M.chinensis sp.nov.在东南沿海广为分布。  相似文献   

14.
腹壁纤毛虫是一种附着在浮游动物上的原生生物,尤其是附着在桡足类和甲壳类上的。而缘毛虫是可以以自由或寄生的形式在世界各沿海地区和港湾处被发现。本研究首次对东印度洋表层至200 m深海域的缘毛类纤毛虫以及其水平分布展开了研究。根据纤毛虫的形态、纤毛结构和茎的形态等的特征,在2014年4月10日至5月13日东印度洋航次的浮游生物拖网样品中鉴定出了五种富腹纤毛虫,海洋钟形虫Vorticella oceanica、交替聚缩虫Zoothamnium alternans、阿拉聚缩虫Zoothamnium alrasheidi、远洋聚缩虫Zoothamnium pelagicum和海洋聚缩虫Zoothamnium marinum。并通过光显微镜和扫描显微镜拍摄的照片,说明了其形态、尺寸和特征。研究结果表明,海洋钟形虫和远洋聚缩虫的分布与硅藻(如,密聚角毛藻Chaetoceros coarctatus)和桡足类(如,短角长腹剑水蚤Oithona brevicornis)等寄主有关,也包括甲藻(如,三角角藻Ceraitum tripose)周围的一些个体。这些附着纤毛虫的分布在东印度洋海域东南区以海洋钟形虫、远洋聚缩虫和海洋聚缩虫为主,而在孟加拉湾北部则有大量的阿拉聚缩虫。这种分布的变化可能是与寄主的分布有关,如桡足类(短角长腹剑水蚤)和硅藻(密聚角毛藻)的分布影响。  相似文献   

15.
Foraminiferal oozes were sampled from the tropical West Pacific seamount and seabed of deep sea,by a remotely operated vehicle(ROV)in December 2014 and March 2016.Using standard morphological method,four Miliolinella species,including Miliolinella obesa,M.circularis,M.suborbicularis and M.subrotunda were described.Among the four species,M.obesa is a new species.It is a large member(about 500μm in length)of the genus.This species is characterized by having a very stout and transverse broadly circular outline,and its body width is greater than the body length.In addition,its chamber demarcations are obscure from the exterior appearance.Three chambers are unclearly visible on one side and two chambers are visible on the opposite.Finally,we provided very detailed taxonomic microphotographs and the ecological distribution information for each species.  相似文献   

16.
As a major aldehyde pollutant widely existing in industry and our daily life, acetaldehyde is more and more harmful to human health. As characteristic habitat niche, bacteria from deep sea environments are abundant and distinctive in heredity, physiology and ecological functions. Thus, the development of acetaldehyde-degrading bacteria from deep sea provides a new method to harness acetaldehyde pollutant. Firstly, in this study,acetaldehyde-degrading bacteria in the deep sea water of the West Pacific Ocean were enriched in situ and in the laboratory respectively, and then the diversity of uncultured bacteria was studied by using 16 S r RNA genes. Then acetaldehyde-degrading strains were isolated from two samples, including enrichment in situ and enrichment in laboratory samples of deep sea water from the West Pacific Ocean using acetaldehyde as the sole carbon source,and then the ability of acetaldehyde degradation was detected. Our results showed that the main uncultured bacteria of two samples with different enrichment approaches were similar, including Proteobacteria,Actinobacteria, Firmicutes, Cyanobacteria, but the structure of bacterial community were significant different.Four subgroups, α, γ, δ and ε, were found in Proteobacteria group. The γ-Proteobacteria was dominant(63.5%clones in laboratory enriched sample, 75% clones in situ enriched sample). The species belonged to γ-Proteobacteria and their proportion was nearly identical between the two enrichment samples, and Vibrio was the predominant genus(45% in laboratory enriched sample, 48.5% in situ enriched sample), followed by Halomonas(9% in situ enriched sample) and Streptococcus(6% in laboratory enriched sample). A total of 12 acetaldehyde-degrading strains were isolated from the two samples, which belonged to Vibrio, Halomonas,Pseudoalteromonas, Pseudomonas and Bacillus of γ-Proteobacteria. Strains ACH-L-5, ACH-L-8 and ACH-S-12,belonging to Vibrio and Halomonas, have strong ability of acetaldehyde degradation, which could tolerate 1.5 g/L acetaldehyde and degrade 350 mg/L acetaldehyde within 24 hours. Our results indicated that bacteria of γ-Proteobacteria may play an important role in carbon cycle of deep sea environments, especial the bacteria belonging to Vibrio and Halomonas and these strains was suggested for their potentials in government of aldehyde pollutants.  相似文献   

17.
东印度洋现生浮游有孔虫   总被引:1,自引:0,他引:1  
A brief morphometric study of the recent planktonic foraminifera in the eastern Indian Ocean was provided with the taxonomic key to species,synonyms,SEM microphotographs of shells and chamber arrays.By recent classification,currently 20 species representing 13 genera and 6 families(Canderinidae,Heterohelicoidae,Hedbergellidae,Higerigerinoidae,Globigerinoidae,and Globorotaloidae)identified from the planktonic material of the eastern Indian Ocean up to a depth of 200 m.Their distribution in water(0–200 m)also reports on the new range of expansion in the eastern Indian Ocean,with Dentigloborotalia anfracta,Hastigerina pelagica,Streptochilus globigerus,Globigerinella calida,Globigerinella adamsi,Orcadia reidelii,Tenuitella parkerae,Tenuitella compressa,reported for the first time in this study area.In general,only around 50 planktonic species are valid worldwide,more specifically the species,e.g.,H.pelagica,G.calida,G.adamsi,S.globigerus,O.riedeli,T.parkerae,T.compressa,which occur in the eastern Indian Ocean to fill the the paucity of the recent regional taxonomic literature and the problematic identification from the eastern Indian Ocean.This work aims to bridge this gap and help scientists,managers,educators and students to identify plankton foraminifers by using species notes and images.  相似文献   

18.
19.
One strain of unicellular greenish algae embedded by mucilage was successfully isolated from equatorial area in the Indian Ocean. Microscopic observation, ultrastructure features and genetic identification confirmed that the strain was closely related to Cyanothece sp., which was a cyanobacteria species with great ecological significance.Cells were solitary with oval or bacilliform shapes. Diameters of this strain were relatively small, ranging from 2.5 to 6.5 μm on average. Ultrastructure of cells was simple. Thylakoids were arranged parietal and keritomized content were observed in the thylakoid region. Various electron-transparent granules with low electron-dense region as well as cyanophycin or glycogen granules-like organelle and carbonxysomes were also observed. For pigment composition, the dominant pigments were chlorophyll a, β-Carotene, Zeaxanthin and an unknown pigment, contributing 23.8%, 26.1%, 14.7% and 15.7% to total pigments respectively. The phylogenetic analysis of16 S rRNA gene and nif H gene confirmed that Strain EIO409 was closely related with Cyanothece sp..  相似文献   

20.
The species compositions of the genus Chironomus in the Curonian and Vistula lagoons are very similar, represented by Ch. plumosus and Ch. balatonicus. In the Curonian Lagoon, in addition to these two species, Ch. muratensis and the first-generation interspecific hybrid Ch. muratensis × Ch. plumosus are also found. In this lagoon, Ch. plumosus occurs most frequently (84%); Ch. balatonicus is considered a subdominant species (24%). In the Vistula Lagoon, on the contrary, the high frequency of occurrence is typical of Ch. balatonicus (89%); for Ch. plumosus, it reaches only 11%. The Ch. plumosus population occupies most of the Curonian Lagoon; Ch. balatonicus is present only in its northern part, from the village of Juodkrante to the city of Klaipeda. In the Vistula Lagoon, Ch. balatonicus is distributed over almost its entire area, while Ch. plumosus is found locally, only near the mouth of the Nogat River. The distribution of the sibling species in the lagoons is determined by the salinity gradient.  相似文献   

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