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Vibrio vulnificus is an opportunistic pathogen widely distributed in estuarine and coastal seawaters. In this study, a culture-free method was developed to rapid detection of V. vulnificus in all seasons, based on loop-mediated isothermal amplification (LAMP) targeting virulent-correlated gene (vcg). The new assay method allows differentiation between the virulent and non-virulent strain of V. vulnificus accurately. This method also allows effective detection of the pathogeny in winter when the bacterium lives in the viable but non-culturable (VBNC) state. A total of 30 costal seawater samples collected in all seasons were used for the evaluation of this method. The results show that the method is sensitive, accurate and convenient. 相似文献
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We present the theoretical results of the electronic band structure of wurtzite GaN films under biaxial strains in the (11-22)-plane. The calculations are performed by the κ• p perturbation theory approach through using the effective-mass Hamiltonian for an arbitrary direction. The results show that the transition energies decrease with the biaxial strains changing from -0.5% to 0.5%. For films of (11-22)-plane, the strains are expected to be anisotropic in the growth plane. Such anisotropic strains give rise to valence band mixing which results in dramatic change in optical polarisation property. The strain can also result in optical polarisation switching phenomena. Finally, we discuss the applications of these properties to the (11-22) plane GaN-based light-emitting diode and lase diode. 相似文献
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Based on the Stokes wave theory, the capillary-gravity wave and the interfacial internal wave in two-layer constant depth''s fluid system are investigated. The fluids are assumed to be incompressible, inviscid and irrotational. The third-order Stokes wave solutions are given by using a perturbation method. The results indicate that the third-order solutions depend on the surface tension, the density and the depth of each layer. As expected, the first-order solutions are the linear theoretical results (the small amplitude wave theoretical results). The second-order and the third-order solutions describe the nonlinear modification and the nonlinear interactions. The nonlinear impact appears not only in the n (n≥2) times'' high frequency components, but also in the low frequency components. It is also noted that the wave velocity depends on the wave number, depth, wave amplitude and surface tension. 相似文献
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Suspended particulate matter (SPM) has been known as an important variable in the organic matter flow of coastal ecosystem. Half of burial carbon in seagrass meadows is contributed by allochthonous sources that compose the SPM such as phytoplankton, seagrass detritus, marine snow aggregates and terrestrially derived particles. Each composition of the SPM contributes different roles and is important to be identified, for instance, the exact contribution of seagrass detritus will be useful for determination of carbon export through the detritus form in seagrass meadows. Here, the SPM of seagrass meadows is studied in Bintan Island and the Selayar Archipelago. The aim of this research is to determine the source origin of the SPM using a stable isotope signature. In order to fulfill this aim, the objectives are defined as: (1) to specify the stable isotope signature (δ13C and δ15N) of the SPM, and (2) to determine the proportional distribution of the SPM''s prospectus sources. The result shows that the possibility of the source origin of the SPM includes a seagrass fraction (Enhalus acoroides and Thalassia hemprichii), terrestrial C4 plant, macroalgae, and terrestrial C3 plant. The SPM lies between the marine- and terrigenous-end members. However, it seems that the SPM is more to be terrigenous-end and allochthonous. According to a Bayesian mixing model, the terrestrial C4 has the highest contribution of the SPM at all sites except Barugaia and Pasi Island in Selayar (i.e., the highest contribution of the SPM is from the detritus of E. acoroides). The second contribution has been contributed by either seagrass detritus (E. acoroides or Th. hemprichii) or terrestrial C3 plant. The finding of this study indicates that there is a strong influence of the terrigenous sources in the SPM of the seagrass meadows. 相似文献
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This paper presents the results of an experimental investigation on the variation in the tension and the distribution of drag force coefficients along flexible risers under vortex-induced vibration (VIV) in a uniform flow for Reynolds numbers (Re) up to 2.2×105. The results show that the mean tension is proportional to the square of the incoming current speed, and the tension coefficient of a flexible riser undergoing VIV can be up to 12. The mean drag force is uniformly and symmetrically distributed along the axes of the risers undergoing VIV. The corresponding drag coefficient can vary between 1.6 and 2.4 but is not a constant value of 1.2, as it is for a fixed cylinder in the absence of VIV. These experimental results are used to develop a new empirical prediction model to estimate the drag force coefficient for flexible risers undergoing VIV for Reynolds number on the order of 105, which accounts for the effects of the incoming current speed, the VIV dominant modal number and the frequency. 相似文献
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Response of phytoplankton to multispecies mariculture: a case study on the carrying capacity of shellfish in the Sanggou Bay in China 总被引：3，自引：0，他引：3
A multispecies model for shellfish polyculture in the Sanggou Bay in China used for large-scale long-line cultivation of the Chinese scallop Chlamys farreri, the Pacific oyster Crassostrea gigas and the kelp Laminaria japonica is presented. The model includes key physical processes which are the transports of matter at the system boundary, and the main biological process that is the primary production and nutrients release from the bottom. By the model, the seasonal fluctuations of phytoplankton biomass and dissolved inorganic nitrogen(DIN) in 1994 are simulated. Furthermore, if the kelp culture scale is kept constant and the Chinese scallop and the Pacific oyster culture scales are adjusted, virtual shellfish farms are funded and responses of phytoplankton to the large-scale shellfish culture are simulated. According to these simulated results, the room limitation, and the hypothesis that shellfish will not grow well if the phytoplankton biomass is less than 8.2 mg/m3, the expandable multiple of scallop culture k and that of oyster culture y are determined as k=-0.276 5y+4.690 5 and 0.133 3k+0.006 6y ≤ 0.667 5, where, k (or y) is equal to 1, the culture scale of scallop (or oyster) is 8.8×109 individuals (or 66 ha, with a density of 59 ind./m2), and the kelp culture scale is 3 300 ha with a density of 12 ind./m2. 相似文献
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This paper investigates the thermal activation behaviour of current in an inhomogeneous Schottky diode with a Gaussian distribution of barrier height by numerical simulation. The analytical Gaussian distribution model predicted that the I--V--T curves may intersect with the possibility of the negative thermal activation of current, but may be contradictory to the thermionic emission mechanism in a Schottky diode. It shows that the cause of the unphysical phenomenon is related to the incorrect calculation of current across very low barriers. It proposes that junction voltage Vj, excluding the voltage drop across series resistance from the external bias, is a crucial parameter for correct calculation of the current across very low barriers. For correctly employing the thermionic emission model, Vj needs to be smaller than the barrier height Ф. With proper scheme of series resistance connection where the condition of Vj > Ф is guaranteed, I--V--T curves of an inhomogeneous Schottky diode with a Gaussian distribution of barrier height have been simulated, which demonstrate normal thermal activation. Although the calculated results exclude the intersecting possibility of I--V--T curves with an assumption of temperature-independent series resistance, it shows that the intersecting is possible when the series resistance has a positive temperature coefficient. Finally, the comparison of our numerical and analytical results indicates that the analytical Gaussian distribution model is valid and accurate in analysing I--V--T curves only for small barrier height inhomogeneity. 相似文献
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BELATTMANIA Zahir CHAOUTI Abdellatif REANI Abdeltif MACHADO Margari ENGELEN Aschwin H SERR&#;O Ester A SABOUR Brahim 《海洋学报(英文版)》2018,37(10):190-194
The littoral paranthurid isopod crustacean Paranthura nigropunctata (Lucas, 1846) is recorded for the first time from the littoral of El Jadida located on the Atlantic northwest coast of Morocco. Specimens were obtained from the invasive brown seaweed Sargassum muticum and the natives Bifurcaria bifurcata and Cystoseira tamariscifolia in January 2015. This new record further confirms a significant southward distribution of P. nigropunctata and contributes to the knowledge of the biogeography of this isopod. Heretofore, the species was only known from the western and eastern Mediterranean and some Atlantic coasts. The present finding is the first of the species from Moroccan Atlantic shores, and suggests that the species may also be present in other coastal localities from Morocco and Africa. Some data on morphology, ecology and spatial distribution of the species are provided. 相似文献
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This paper studies the viscid and inviscid fluid resonance in gaps of bottom mounted caissons on the basis of the plane wave hypothesis and full wave model. The theoretical analysis and the numerical results demonstrate that the condition for the appearance of fluid resonance in narrow gaps is kh=(2n+1)π (n=0, 1, 2, 3, …), rather than kh=nπ (n=0, 1, 2, 3, …); the transmission peaks in viscid fluid are related to the resonance peaks in the gaps. k and h stand for the wave number and the gap length. The combination of the plane wave hypothesis or the full wave model with the local viscosity model can accurately determine the heights and the locations of the resonance peaks. The upper bound for the appearance of fluid resonance in gaps is 2b/L<1 (2b, grating constant; L, wave length) and the lower bound is h/b≤1. The main reason for the phase shift of the resonance peaks is the inductive factors. The number of resonance peaks in the spectrum curve is dependent on the ratio of the gap length to the grating constant. The heights and the positions of the resonance peaks predicted by the present models agree well with the experimental data. 相似文献
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胶州湾是位于新华夏隆起带次级构造单元胶南隆起区和胶莱凹陷的交接部的断陷盆地,团岛——薛家岛海底隧道开挖段有大量火成岩出露。本文对采集于海底隧道的8件岩石样品进行了岩石学和地球化学研究,按岩性将其分为偏基性岩类和偏酸性岩类两类。其中除样品F4-5(辉绿岩)外,偏基性岩类均落入岛弧型钙碱性玄武岩区域内;而偏酸性样品均落入火山弧花岗岩区域,显示其原岩起源于同一种火山弧的环境。稀土元素标准化曲线表明,其岩石轻重稀土元素分馏比较明显,其LREE/HREE也显示样品的分异作用较强。岩石样品的地球化学特征研究证明,研究区域的玄武质岩体为遭受地壳混染的岛弧型性玄武岩,应为在燕山晚期花岗质脉岩沿着青山群中构造薄弱带侵入,与地壳物质发生混染作用形成的。 相似文献
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对虾工厂化育苗在我国沿海已经普及,对虾人工养殖也在沿海地区蓬勃发展,到1992年养殖面积已达200多万亩,人工育苗场全国约有上千个。养殖对虾已成为我国水产业中规模最大、经济效益最高的水产品。但近几年来沿海地区的工业飞速发展,沿岸海水均受到了不同程度的污染,各对虾育苗场卵孵化率普遍下降。今年我国养殖对虾又出现了大面积暴发性死亡,原因是复杂的,但作者认为,治虾病之本应是治水质。众所周知,一切生物的幼体阶段都是最敏感的时期,海水中的金属离子对对虾幼体生长的影响已有报道(吴彰宽等,1988; Lawrence et al.,1981),但其实验环境、方法及条件各有不同。作者于1992年5月至1993年4月在海阳育苗场作了现场实验,本文根据实验结果分析和研究了Zn、Cd、Pb、Cu金属离子对中国对虾受精卵及无节幼体生物毒性的影响;不同pH值海水加入不同浓度的混合金属离子,对无节幼体生长的毒性影响;不同盐度的海水加入不同浓度的混合金属离子对无节幼体生长毒性的影响。 相似文献
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起源于台湾东南和非律宾东北海域的黑潮,在沿台湾东岸北上的过程中,其表层流轴的位置有着明显的季节变异。本文根据所搜集到的资料(主要是GEK资料),粗略分析了台湾以东及东海南部海域黑潮表层流轴的季节变异,并对引起变异的原因作了初步探讨,认为季风和海底地形的共同作用,形成了该海域中黑潮特有的路径特征,而季风是引起黑潮表层流轴季节变异的主要原因。 相似文献
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MINERALOGY OF COASTAL DEPOSITS OF NORTH-WESTERN SEA OF JAPAN 总被引：1，自引：0，他引：1
Distribution of heavy, light and clay minerals in Late Pleistocene—Holocene deposits from different coastal environments (beaches, lagoons, dunes, relict lakes, ria bays and inlets, open inlets) was analysed. It was established that mineralogical composition of coastal deposits was a result of provenance and its hypergenous changes, transformation of material within transit zone, and accumulative conditions of inshore zone. Contents of stable minerals increase in deposits of the inlets confined to mouths of the small streams and in horizons of the sections, formed during regressive phases. Unstable minerals amount is higher in deposits of inlets, confined to mouths of the large river. The clay mineral fraction of shelf sediments consists mostly of illite. Authigenous pyrites dominate in deposits of ria bays, lagoons and relict lakes, formed during transgressive phases. Volcanic glass, found in deposits, is product of Holocene eruptions of Baegdusan (Baitushen) Volcano. 相似文献
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１９９３年分析了不同大小斑节对虾肌肉中游离氨基酸含量和肌肉蛋白质 的氨基酸组成。虾体肌肉中有各种游离氨基酸存在，但数量很少，而且比例与肌肉 中结合氨基酸不一致。不同大小斑节对虾，其肌肉中氨基酸组成基本一样，不同的 是小虾的半胱氨酸、蛋氨酸含量比中虾、成虾的低；成虾的缬氨酸、异亮氨酸含量比 小虾、中虾的低，而酪氨酸、苯丙氨酸、天门冬氨酸较高。随着虾体的长大，甘氨酸、 丙氨酸的含量逐渐下降，而脯氨酸的含量升高。雌雄虾的氨基酸组成基本一致，只 是雄虾的甘氨酸含量偏低，精氨酸含量偏高。 相似文献
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1969年电导盐度定义确立以后,电导测盐法迅速普遍地替代了氯度滴定法,成为日常最广泛的测定海水盐度的方法。氯度与盐度(或相对电导率)已成为两种独立的海洋参数,而国际标准海水为各国海洋工作者提供海水氯度滴定及盐度测定的标准。Park,K.(1964),Poisson,A.,Millero等人(1977)和Culkin,F.(1978)分别对国际标准海水的氯度盐度和电导盐度作了比测研究。 相似文献
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青岛市中港东区码头工程水下爆岩工程量１．３万m^3,施工中采用了多段微差爆破,毫秒延迟爆破,导爆管起爆等多项为先进和成熟的施工技术,合理的施工组织,完善的施工管理,保证了工程质量,加快了工程进度。 相似文献