共查询到19条相似文献，搜索用时 171 毫秒
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Studies were conducted to investigate the non-specific immune responses and disease-resistance of juvenile turbot Scophthalmus maximus,cultured at four different salinities(8,20,32 and 40) . Three concentrations(3.75 × 10 7,3.75 × 10 8 and 3.75 × 10 9 CFU/ml) of Vibrio anguillarum suspension were employed at each salinity to determine the 4-day LD 50 . The serum lysozyme activity,the alternative complement pathway activity(ACH50) and the phagocytosis percentage of head kidney in turbot were tested at 24,48 and 72 h post-challenge of V. anguillarum(1.1 × 10 8 CFU/ml,0.1 ml) ,respectively,to evaluate the non-specific immune responses at the selected rearing salinities. Fish reared at salinity 20 had the lowest mortality,namely,the highest 4-day LD 50 value(8.88 ± 0.17) . Besides,the lysozyme activity,ACH50 and the phagocytosis of turbot were the highest at the salinity 20,but with the lowest at the salinity 40 treatment regardless of sampling time. In addition,the non-specific immune activities kept increasing within 72 h post-challenge of V. anguillarum,except that the lysozyme activity increased from 24 to 48 h,and then decreased from 48 to 72 h at 40 significantly. These results together indicate that rearing in intermediate salinity(20) was able to enhance the immunity and disease-resistance of turbot. 相似文献
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CHENG Huihui LIU Wenbin YUAN Xiangyang JIA Erteng ZHANG Dingdong JIANG Guangzhen 《海洋湖沼学报（英文）》2020,(3):869-882
One hundred and sixty crabs(average initial weight:51.32±0.08 g)were fed with four experimental diets containing cottonseed meal protein hydrolysate(CPH)at 0%(CPH0,control),0.3%(CPH0.3),0.6%(CPH0.6),and 1.2%CPH(CPH1.2).The experiment results show that no difference was observed in specific growth rate and survival rate of crabs fed with CPH diet.Moisture content of crabs fed with CPH0.6 diet was significantly reduced than that of the CPH0 group.Superoxide dismutase and catalase activities of crabs fed with CPH0.6 diet were significantly increased and the difference was not significant between the CPH0.3 and CPH0.6 groups.Malondialdehyde content of CPH0.3 group was significantly lower than that of the CPH0 group.Lysozyme,alkaline phosphatase,and acid phosphatase activities of CPH0.3 diet crabs were significantly higher than that of the CPH0 group.Glutamic-pyruvic transaminase activity of crabs fed with CPH0.3 diet was significantly decreased compared to the CPH0 group.The relative expression levels of Toll1,Toll2,MyD88,LITAF,and ILF-2 of crabs fed with CPH0.3 diet were significantly higher than that of the CPH0 group.The expression level of SOCS2 showed an opposite pattern.After CPH perfusion,the expression levels of SOCS2 and Toll1 in intestine at time 3 h and SOCS2 in hepatopancreas at time 18 h increased significantly to the highest value.The expression level of Toll2,MyD88,LITAF,decreased at times 6 h,6 h,12 h,respectively,then increased gradually.Therefore,supplementation of dietary CPH could improve antioxidant capacity and immune function;the appropriate supplement dosage of CPH for crab could be 0.3%-0.6%.Furthermore,the short-term CPH stimulation could significantly increase or decline the expression levels of immune-related genes at different times after CPH perfusion. 相似文献
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Dot enzyme-linked immunosorbent assay （dot-ELISA）, indirect ELISA and Westem blot were performed to detect the virulent protease secreted by Vibrio anguillarum which was isolated from the diseased left-eyed flounder, Paralichthys olivaceous. Sensitivity results showed that dot-ELISA is a more sensitive, rapid and simple technique for the protease detection. The minimal detectable amount of protease is about 7 pg in the dot-ELISA test, while 7.8 ng in the indirect ELISA and 6.25 ng in the Westem blot respectively. Protease could be detected 2 h after incubation of V. anguillarum in the 2216E liquid medium but enzyme activity was very low at that period. From 6 to 12 h, the amount and enzyme activity of protease increased markedly and reached maximum at stationary phase. Analysis of serum samples periodically collected from the infected flounders showed that after 2 h of infection by V. anguillarum, the pathogenic bacteria could be detected in the blood of the infected flounders but no protease was found. It was 5-6 h after infection that the protease was detected in blood and then the amount increased as infection advanced. Quantitative detection of protease either incubation in the medium or from the blood of infected flounders could be accomplished in virtue of positive controls of quantificational protease standards （＂marker＂） so that the alterations ofprotease secretion both in vitro and in vivo could be understood generally. In addition, the indirect ELISA and dot-ELISA were also performed to detect V. anguillarum cells. Results indicated that the sensitivity of indirect ELISA to bacteria cells is higher than that of the dot-ELISA, and that the minimal detectable amount is approximately 10^4 cell/mL in the indirect ELISA, while 10^5 cell/mL in the dot-ELISA. 相似文献
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Penaeidin from Chinese shrimp (Fenneropenaeus chinensis) has proved to be one of the most important antimicrobial peptides in the bodies of animals. The relative quantitative real-time PCR method is developed to study through time, the mRNA expression profile of penaeidin in the muscle and haemoeyte tissue of Chinese shrimp infected with vibrio (Vibrio anguillarum) and WSSV (white spot syndrome virus). Research results showed that the same pathogens infection experiments produced similar gene expression profile in different tissues while different expression profiles appeared in the same tissues infected by different exterior pathogens. In vibrio infection experiments, a “U” like expression profile resulted. Expression levels of ponaeidin increased and surpassed the non-stimulated level, indicating that penaeidin from Chinese shrimp has noticeable antimierobial activities. In WSSV infection experiments, the expression profile appeared as an inverse ““““U““““ with the expression ofpenaeidin gradually decreasing to below baseline level after 24 h.The expression of antimierobial peptides gene in mRNA level in response to virus infection in shrimp showed that international mechanisms ofvirns to haemoeytes and microbial to haemoeytes are completely different. Decline of ponaeidins expression levels may be due to haemoeytes being destroyed by WSSV or that the virus can inhibit the expression ofponaeidins by yet undiscovered modes. The expression profiles of penaeidin in response to exterior pathogen and the difference of expression profiles between vibrio and WSSV infection provided some clues to further understanding the complex innate immune mechanism in shrimp. 相似文献
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Recombinant bacteria secreting the transmembrane-truncated region of white spot syndrome virus (WSSV) envelope protein VP28 named DhpVB were constructed, using live Escherichia coli as a deliverer and a constitutive secretory expression plasmid as the expression vector. With the ability to deliver recombinant protein products outside, DhpVB could make VP28 directly interact with the shrimp when injected into shrimp. In order to test the protection potential, live DhpVB were injected into the marine shrimp Exopalamon carincauda Holthuis for three times and WSSV challenge was performed twice simultaneously at the last two injections of the bacteria. The results showed that DhpVB displayed statistically significant advantage over bacteria without VP28 after the second challenge with an average RI (Resistance index) of 0.67 ± 0.08, compared with 0.41 ± 0.09 of bacteria without VP28 (P <0.05). The data suggested that a quasi-immune response may exist in E. carincauda and live bacteria carrying VP28 could enhance the shrimp resistance against WSSV. 相似文献
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A histological and ultrastructural study was performed on the development of the head kidney, thymus, and spleen in Pseudosciaena crocea aging in range from just hatching to the 60th day after hatching (DAH). Head kidney was first present on the 3rd DAH. Primordial haemopoietic stem cells were first observed in the head kidney which rapidly differentiate into different cellular types. Progenitor spleen was present on the 4th DAH, located close to the gut, which soon became rich in blood capillaries, red blood cells and thrombocytes. The thymus was obvious on the 4th DAH, and was located on either side of the upper corner of the opercular cavity, closely under the membrance of the opercuhr cavity. The thymus was the last lymphoid organ to appear but showed a quick development. This organ seemed to originate from haemopoietic stem cells migrating from the head region of the kidney. The thymus consisted of outer thymocytic and inner epithelioid zones. There was no obvious demarcation between them, but both zones were visible. Small lymphocytes appeared, or lymphoid organs become lymphoid, in the sequence thymus, head kidney and spleen. Only a small number of lymphocytes appeared in the later stages. Non-specific systems may play an important role in the immunocompetence mechanisms of P. crocea during its early development stage. 相似文献
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Chunyan Zhao Shihong Xu Yifan Liu Chengcheng Feng Yongshuang Xiao Yanfeng Wang Qinghua Liu Jun Li 《海洋学报(英文版)》2022,41(1):84-98
In most fish,reproduction is seasonal or periodic under the suitable conditions.In turbot(Scophthalmus maximus)farms,one of the most economically important marine flatfish species,changes in daylength could cause changes in the spawning time.In this study,to characterize the regulation of reproductive physiology following light signals,three melatonin receptors(Mtnr)investigated in turbot were named sm Mtnr1,sm Mtnr2,and sm Mtnr1 c.Distinct expression profiles demonstrated that Mtnr m RNAs were concentrated in the brain(as detected in the hypothalamus(Hy)and mesencephalon(Me)),gonad and eye.The most abundant Mtnr1 and Mtnr2 m RNA expression levels were detected in the central nervous system at the beginning of the breeding season,suggesting that Mtnr1 and Mtnr2 may play vital roles in the regulation of turbot gonadal development.In addition,the melatonin profiles gradually increased and reached to the highest level at the spawning stage,indicating that melatonin is a potent hormone in the regulation of fish oocyte growth and maturation.The results of this study suggested that melatonin is the primary factor that transduces the light signal and regulates the physiological functions of turbot seasonal reproduction.Moreover,the results of this study may establish a foundation for further research seeking to identify fish melatonin receptors involved in the gonadal development and gamete maturation. 相似文献
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为选育高成活率的大菱鲆(Scophthalmus maximus)抗鳗弧菌(Vibrio anguillarum)群体,对30个大菱鲆选育二代家系(F2)和1个普通养殖群体进行鳗弧菌攻毒实验(周期为14 d),统计分析死亡率。选取死亡率不同的6个选育家系和普通养殖群体构成7个实验组,采用半定量-聚合酶链反应(semi-quantitative polymerase Chain reaction,RT-PCR)技术对它们肝脏、脾脏、头肾的相关免疫因子——溶菌酶(Lysozyme)、抗菌肽(Hepcidin)、热激蛋白70(HSP70)、热激蛋白90(HSP90)、免疫球蛋白(Ig M)、C-型凝集素(C-type lectin)、Lily-型凝集素(Lily-type lectin)的表达量开展研究,并应用SPSS 16.0软件对攻毒前后各免疫因子的表达量与攻毒后存活率之间的相关性进行分析。研究结果表明:攻毒前后选育家系幼鱼肝脏、脾脏、头肾中7个免疫因子的表达量普遍高于普通养殖幼鱼,且在7个实验组中,攻毒后的表达量相较攻毒前均呈现下降趋势;攻毒前,肝脏中lysozyme、Ig M的表达量与存活率为极显著性正相关(P0.01),HSP70、HSP90、C-type lectin、Lily-type lectin的表达量与存活率为显著性正相关(P0.05);脾脏中,Hepcidin、HSP70和HSP90的表达量与存活率为极显著性正相关(P0.01);头肾中,HSP70的表达量与存活率为显著正相关(P0.05)。综上,可以推断选育家系相对于普通养殖群体大菱鲆抗鳗弧菌性能更强,且7种免疫因子在鳗弧菌感染鱼体的过程中发挥重要的抗感染作用;从F2中获得一个抗鳗弧菌性能较强的家系(23号),可用于今后大菱鲆抗鳗弧菌品系的选育指导工作,为大菱鲆高成活率群体的选育提供参考资料和理论依据。 相似文献
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实验采用末端快速扩增(RACE)技术从大菱鲆脾脏cDNA文库中筛选得到了免疫球蛋白轻链IgL的全长cD-NA片段。该序列包含47 bp的5′末端非编码区(5′UTR),738 bp的开放阅读框(ORF)和202 bp 3′UTR,整个开放阅读框编码246个氨基酸。系统发生分析表明大菱鲆IgL基因与五条鰤的IgL基因起源关系最近。RT-PCR分析表明,大菱鲆IgL基因只在正常脾脏、肾脏和头肾组织中表达;IgL在大菱鲆胚胎发育细胞期就已开始表达,在大菱鲆胚胎尾芽期和体节期表达持续增强;在鳗弧菌感染大菱鲆脾脏和头肾早期均检测到IgL基因强烈表达,后期表达逐渐减弱;大菱鲆胚胎细胞(TEC)在用鳗弧菌感染48h后,IgL开始表达。这些结果均表明IgL基因在大菱鲆免疫应答中起着重要作用。 相似文献
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采用基因工程的方法,将副溶血弧菌的热稳定直接溶血素基因tdh2和鳗弧菌外膜蛋白基因ompU进行融合,在大肠杆菌中得到表达,并利用该融合基因构建二联DNA疫苗pEGFP．N1／tdh2-ompU。用DNA疫苗按10和50μg／尾的剂量通过肌肉注射免疫大菱鲆,对大菱鲆抵抗致病性副溶血弧菌和鳗弧菌的免疫效果进行研究。结果表明... 相似文献
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鳗弧菌感染对斑节对虾免疫相关指标的影响 总被引：1，自引：0，他引：1
为了研究鳗弧菌(Vibrio anguillarum)对斑节对虾(Penaeus monodon)免疫相关指标的影响,分别对斑节对虾注射生理盐水和鳗弧菌,测定了不同时间点肝胰腺和鳃中总抗氧化能力(T-AOC)、溶菌酶(LSZ)和诱导型一氧化氮合酶(i NOS)活性以及一氧化氮(NO)含量的变化。结果显示,与对照组相比,感染鳗弧菌后,肝胰腺和鳃中T-AOC活性分别于6和12 h达到最大值(P0.05),随后逐渐降低;肝胰腺和鳃中LSZ活性分别于6和12 h升高至最大值,随后均于24 h显著低于对照组(P0.05);肝胰腺和鳃中NO含量分别于6和3 h达到最大值,随后肝胰腺中NO含量于24 h降低至最小值,而鳃中NO含量于6和24 h虽有降低,但仍显著高于对照组;肝胰腺中i NOS对感染反应较为敏感,活性于3 h达到最大值,而鳃中i NOS活性于6 h开始显著上升,并于12 h达到最大值(P0.05)。研究表明,长时间的鳗弧菌感染对斑节对虾免疫相关指标有显著影响;T-AOC、LSZ、NO和i NOS对鳗弧菌感染均反应敏感,可以作为弧菌病诱发的监测指标。 相似文献
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应用组织切片研究了大菱鲆(Scophthalmus maximus)初孵仔鱼至60 日龄幼鱼免疫器官的发育过程。结果表明, 免疫器官原基出现的先后顺序是头肾、脾脏和胸腺。1 日龄仔鱼可以观察到头肾原基, 包含未分化的造血干细胞。5 日龄时, 脾脏原基出现, 其淋巴化开始于27 日龄且发育速度较慢。13 日龄时, 大菱鲆仔鱼胸腺原基出现, 且发育速度较快, 分为外区和内区。在大菱鲆早期发育过程中, 胸腺和头肾之间出现细胞迁移现象。免疫器官淋巴化的顺序是胸腺、头肾和脾脏。在免疫器官发育后期, 脾脏和头肾中均发现了黑色素-巨噬细胞中心(MMCs), 脾脏中较丰富, 但在胸腺中尚未发现。在大菱鲆仔鱼早期发育阶段, 免疫器官尚未发育完善, 非特异性防御机制起着重要的免疫作用。 相似文献
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通过给大菱鲆(Scophthalmus maximus)注射不同浓度的紫锥菊(Echinacea purpurea)提取物,测定其非特异性免疫指标的变化水平,研究其对大菱鲆的头肾内非特异性免疫分子基因相对表达量的影响,进而为其在大菱鲆养殖生产过程中推广应用提供科学依据。试验选取体重(250±20)g的大菱鲆作为试验动物,分别注射10、20、40mg/m L浓度的紫锥菊提取物,试验共进行28d,注射试验结束后,分别从高、中、低剂量组及空白对照组选出6尾大菱鲆,分别取出大菱鲆的头肾并提取总RNA。采用?Ct法进行目标基因的荧光定量分析,再应用SPSS17.0软件对获得的实验数据进行单因素方差分析。研究结果表明,紫锥菊提取物能够不同程度地提高大菱鲆头肾内非特异性免疫分子基因相对表达量。注射紫锥菊提取物28d后对大菱鲆头肾中Lysozyme基因相对表达量的影响显著(P0.05),对C3补体基因相对表达量的影响极显著(P0.01),对Transferrin基因相对表达量的影响显著(P0.05),对TGF-β1基因相对表达量的影响极显著(P0.01),对IL-1h基因相对表达量的影响极显著(P0.01),本试验研究表明,紫锥菊提取物能够显著提高大菱鲆头肾内非特异性免疫分子基因的相对表达量。 相似文献
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应用荧光定量PCR技术, 检测了TLR21 基因在牙鲆(Paralichthys olivaceus)感染迟缓爱德华 氏菌(Edwardsiella tarda)后, 在0 h、1 h、3 h、6 h、12 h、1 d、3 d、6 d 后, 在心脏、肝脏、脾脏、 头肾、鳃、小肠、肌肉和血的时空表达特征, 并探讨了它们与牙鲆先天免疫反应的关系。结果表明, 大 多组织在感染病原6 h 后TLR21 基因表达明显上调, 尤其是头肾和小肠。头肾6 h 的表达量达到了 对照组的59.3 倍, 小肠6 h 的表达量为对照组的38.6 倍。迟缓爱德华氏菌感染引起牙鲆体内各组织 中TLR21 的上调表达和变化, 为研究牙鲆对迟缓爱德华氏菌的防御机制提供了理论依据。 相似文献
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本文测定了大黄鱼C3(L.c-C3)和C4(L.c-C4)基因的c DNA全序列。结果表明,L.c-C3和L.cC4序列全长分别为4962bp和5088bp,分别编码1653和1695个氨基酸,N端信号肽序列分别为23和19个氨基酸。推导的氨基酸序列结构分析表明大黄鱼C3和C4与已报道的补体C3、C4同样都具有在功能上比较重要的残基以及保守的硫酯区。分子进化分析表明,L.c-C3和L.c-C4分别与鮸鱼C3、C4的氨基酸同源性最高。实时荧光定量PCR结果显示,L.c-C3和L.c-C4在健康大黄鱼的肝脏、脾脏、肠、鳃、心脏、脑、肌肉和胃这8种组织中都有表达,其中肝脏的表达量最高。在大黄鱼胚胎不同发育时期(从2细胞期到初生仔鱼)中,L.c-C3在各个阶段没有明显的变化,而L.c-C4的表达量有明显升高。溶藻弧菌(Vibrio alginolyticus)侵染的大黄鱼肝脏和脾脏中,L.c-C3和L.c-C4的m RNA表达量均明显上调。该结果表明,大黄鱼肝组织C3和C4基因表达变化与溶藻弧菌的侵染密切相关,揭示了C3和C4在大黄鱼抗细菌免疫反应中具有重要的作用。 相似文献