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1.
Phytoplankton NH4+ and NO3− uptake was examined along the longitudinal salinity gradient of the Delaware Estuary over several seasonal cycles using 15N-tracer techniques. Saturated nitrogen uptake rates increased directly with water temperature and reached a maximum of 380 nmol Nl−1h−1 during summer. This temperature dependence was related primarily to changes in the rate of maximum chlorophyll specific uptake, which varied exponentially between 2 and 70 nmol N [μg Chl h]−1 over a temperature range of 2–28°C. Despite these high uptake rates, balanced growth (C:N7:1) could be maintained over the diel light cycle only by highly efficient nitrogen uptake at low light intensities and dark uptake below the photic zone and at night (dark UPTAKE=25% maximum uptake).Ammonium fulfilled 82% of the annual phytoplankton nitrogen demand in the estuary despite dominance of NO3− in the ambient dissolved inorganic nitrogen pool. The predominance of NH4+ uptake occurred because of the general suppression of NO3− assimilation at NH4+ concentrations in excess of 2 μ . This suppression, however, was not as universal as has been reported for other systems, and it is suggested that the extremely high NO3− concentrations found in the estuary contribute to this pattern. Nitrate was a significant source of nitrogen only during periods of high phytoplankton production in summer, and when NH4+ concentrations were low towards the end of the spring bloom.  相似文献   

2.
We report the first application of a biogeochemical model in which the major elemental composition of the phytoplankton is flexible, and responds to changing light and nutrient conditions. The model includes two phytoplankton groups: diatoms and non-siliceous picoplankton. Both fix C in accordance with photosynthesis-irradiance relationships used in other models and take up NO3− and NH4+ (and Si(OH)4 for diatoms) following Michaelis-Menten kinetics. The model allows for light dependence of photosynthesis and NO3− uptake, and for the observed near-total light independence of NH4+ uptake and Si(OH)4 uptake. It tracks the resulting C/N ratios of both phytoplankton groups and Si/N ratio of diatoms, and permits uptake of C, N and Si to proceed independently of one another when those ratios are close to those of nutrient-replete phytoplankton. When the C/N or Si/N ratio of either phytoplankton group indicates that its growth is limited by N, Si or light, uptake of non-limiting elements is controlled by the content of the limiting element in accordance with the cell-quota formulation of Droop (J. Mar. Biol. Ass. U.K 54 (1974) 825).We applied this model to the Bermuda Atlantic Time-series Study (BATS) site in the western Sargasso Sea. The model was tuned to produce vertical profiles and time courses of [NO3−], [NH4+] and [Si(OH)4] that are consistent with the data, by adjusting the kinetic parameters for N and Si uptake and the rate of nitrification. The model then reproduces the observed time courses of chlorophyll-a, particulate organic carbon and nitrogen, biogenic silica, primary productivity, biogenic silica production and POC export with no further tuning. Simulated C/N and Si/N ratios of the phytoplankton indicate that N is the main growth-limiting nutrient throughout the thermally stratified period and that [Si(OH)4], although always limiting to the rate of Si uptake by diatoms, seldom limits their growth rate. The model requires significant nitrification in the upper 200 m to yield realistic time courses and vertical profiles of [NH4+] and [NO3−], suggesting that NO3− is not supplied to the upper water column entirely by physical processes. A nitrification-corrected f-ratio (fNC), calculated for the upper 200 m as: (NO3− uptake—nitrification)/(NO3− uptake+NH4+ uptake) has annual values ranging from only 0.05–0.09, implying that 90–95% of the N taken up annually by phytoplankton is supplied by biological regeneration (including nitrification) in the upper 200 m. Reported discrepancies between estimates of organic C export based on seasonal chemical changes and POC export measured at the BATS site can be almost completely resolved if there is significant regeneration of NO3− via organic-matter decomposition in the upper 200 m.  相似文献   

3.
On the basis of mass balance calculations performed for nitrogen (N) uptake experiments in the Southern California Bight (SCB), it has been suggested that a significant portion of dissolved inorganic N (DIN) uptake results in the production of dissolved organic N (DON). To investigate this process, the fate of ammonium (NH4+) and nitrate (NO3−) uptake was quantified within the euphotic zone at three coastal stations in the SCB using 15N tracer techniques. Several trends in the fate of DIN and the production of DON were observed. First, production of particulate N (PN), from both NH4+ and NO3−, was quantitatively more important in near surface waters, while DON release dominated within the nitracline. Second, the percentage of gross N uptake released as DON was generally higher when NO3−, rather than NH4+, was the substrate. Third, the percentage of N released as DON was higher at night, relative to the day. Fourth, rates of DON release were significantly correlated to NH4+ regeneration, suggesting that similar mechanisms are responsible for both processes—presumably grazing. The results of this study indicate that the DON pool is a sink for DIN uptake on the time scale of hours. One implication of this finding is that new production estimates based on 15NO3− uptake rates will likely underestimate particle flux out of the surface layer because the rate of NO3− uptake is underestimated due to loss of DO15N during the incubation. On time scales of months to years, however, the N that is taken up as NO3− and released as DON will likely contribute to export flux via incorporation of the dissolved phase during seasonal mixing into sinking particles or transport. The export of DON on these time scales argues for the use of gross uptake rates to calculate f-ratios.  相似文献   

4.
Uptake of inorganic carbon and ammonium by the plankton community of three North Carolina estuaries was measured using 14C and 15N isotope methods. At 0% light, C appeared to be lost via respiration, and at increasing light levels uptake of inorganic carbon increased linearly, saturated (mean Ik = 358±30 μEin m−2 s−1), and frequently showed inhibition at the highest light intensities. At 0% light NH4+ uptake was significantly greater than zero and was frequently equivalent to uptake in the light (light independent); at increasing light levels NH4+ uptake saturated (mean Ik = 172±44 μEin m−2 s−1) and frequently indicated strong inhibition. Light-saturated uptake rates of inorganic carbon and NH4+ were a function of chlorophyll a (r2 = 0·7−0·9); average assimilation numbers were 625 nmol CO2 (μg chl. a)−1 h−1 and 12·9 nmol NH4+ (μg chl. a)−1 h−1 and were positively correlated with temperature (r2 = 0·3−0·7). The ratio of dark to light-saturated NH4+ uptake tended to be near 1·0 for large algal populations at low NH4+ concentrations, indicating near light independence of uptake; whereas the ratio was lower for the opposite conditions. These data are interpreted as indicative of nitrogen stress, and it is suggested that uptake of NH4+ deep in the euphotic zone and at night are mechanisms for balancing the C:N of cellular pools. A 24-h study using summed short-term incubations confirmed this; the cumulative C:N of CO2 and NH4+ uptake during the daylight period was 10–20, whereas over the 24-h period the ratio was 6 due to dark NH4+ uptake. Annual carbon and nitrogen primary productivity were respectively estimated as 24 and 4·0 mol m−2 year−1 for the South River estuary, 42 and 7·3 mol m−2 year−1 for the Neuse River estuary, and 9·6 and 1·6 mol m−2 year−1 for the Newport River estuary.  相似文献   

5.
Nutrient-enrichment bottle experiments in the northwestern Indian Ocean surface waters were conducted to investigate phytoplankton growth following enrichments with either NH4+, NO3−, Fe or Fe + NO3−. Stimulation of phytoplankton growth could be achieved by the addition of either NH4+ or NO3− under the ambient Fe concentrations, but the most significant increases in Chl a, POC, and cell densities were observed in the Fe + NO3−-amended culture. Iron addition caused more rapid responses of phytoplankton growth in the Fe + NO3− treatment than those in the NO3− and NH4− treatment. However, the Fe-enrichment treatment revealed minimal growth of phytoplankton because of severe major nutrient deficiency and was similar to the control treatment. Increases in the cell density of diatoms and spherical phytoplankton cells (< 10 μm) were significant in the NH4+-enriched samples, whereas NO3− enrichment alone had little effect on the diatoms. Simultaneous addition of Fe and NO3− stimulated maximal growth of phytoplankton, in particular in diatoms, coccolithophorids and Phaeocystis type colonies. However, the dominance of coccolithophorids and Phaeocystis type colonies in the Fe + NO3− treatment may be interpreted as resulting from Si-limitation. The high N/P ratio for phytoplankton nutrient uptake in the N-amended culture indicates the possibility of some P-limited growth. From these results, we conclude that in the northwestern Indian Ocean, Fe and major nutrients are co-limiting phytoplankton production during the northeast monsoon. Iron appeared to affect the ability of phytoplankton to respond quickly to transient nutrient inputs.  相似文献   

6.
The seasonal dynamics of inorganic nutrients and phytoplankton biomass (chlorophyll a), and its relation with hydrological features, was studied in the NW Alboran Sea during four cruises conducted in February, April, July and October 2002. In the upper layers, the seasonal pattern of nutrient concentrations and their molar ratios (N:Si:P) was greatly influenced by hydrological conditions. The higher nutrient concentrations were observed during the spring cruise (2.54 μM NO3−, 0.21 μM PO43− and 1.55 μM Si(OH)4, on average), coinciding with the increase of salinity due to upwelling induced by westerlies. The lowest nutrient concentrations were observed during summer (<0.54 μM NO3−, 0.13 μM PO43− and 0.75 μM Si(OH)4, on average), when the lower salinities were detected. Nutrient molar ratios (N:Si:P) followed the same seasonal pattern as nutrient distribution. During all the cruises, the ratio N:P in the top 20 m was lower than 16:1, indicating a NO3− deficiency relative to PO43−. The N:P ratio increased with depth, reaching values higher than 16:1 in the deeper layers (200–300 m). The N:Si ratio in the top 20 m was lower than 1:1, excepting during spring when N:Si ratios higher than 1:1 were observed in some stations due to the upwelling event. The N:Si ratio increased with depth, showing a maximum at 50–100 m (>1.5:1), which indicates a shift towards Si-deficiency in these layers. The Si:P ratio was much lower than 16:1 throughout the water column during the four cruises. In general, the spatial and seasonal variation of phytoplankton biomass showed a strong coupling with hydrological and chemical fields. The higher chlorophyll a concentrations at the depth of the chlorophyll maximum were found in April (2.57 mg m−3 on average), while the lowest phytoplankton biomass corresponded to the winter cruise (0.74 mg m−3 on average). The low nitrate concentrations together with the low N:P ratios found in the upper layers (top 20 m) during the winter, summer and autumn cruises suggest that N-limitation could occur in these layers during great part of the year. However, N-limitation during the spring cruise was temporally overcome by nutrient enrichment caused by an intense wind-driven upwelling event.  相似文献   

7.
The Loire estuary has been surveyed from 1982 to 1985 by 13 isochronous longitudinal profiles realized at low tide. Nutrient (SiO2, NO3−, NH4+, PO3−4, particulate organic carbon or POC) patterns are very variable depending on the season, the estuarine section [river, upper-inner estuary, upstream of the fresh-water-saline-water interphase FSI, the lower-inner estuary characterized by the high turbidity zone (HTZ), the outer estuary] and the river discharge. Biological processes are dominant. In the eutrophied River Loire (summer pigment > 100 μg l−1), the high algal productivity (algal POC > 3 mg l−1) results in severe depletion of SiO2, PO43−, NO3−. The enormous biomass (55 000 ton algal POC/year) is degraded in the HTZ where bacterial activity is intense. As a result, there is generally a regeneration of dissolved SiO2 and PO43−, a marked NH4+ maximum, while NO3− is conservative or depleted when the HTZ is nearly anoxic. Other processes can be considered including pollution from fertilizer plans (PO43−, NH4+) and from a hydrothermal power plant (NH4+). In the less turbid outer estuary, nutrients are generally conservative. Major variations of concentrations are observed in the lowest chlorinity section (Cl− < 1 g kg−) and also upstream the FSI, defined here as a 100% increase in Cl−. Nutrient inputs to the ocean are not significantly modified for SiO2 and NO2−, but are increased by 70% and 180% for PO43− and NH4+ and depleted by 60% for POC. Odd hydrological events, especially some floods, may perturbate or even mask the usual seasonal pattern observed in profiles.  相似文献   

8.
Benthic fluxes of dissolved inorganic nitrogen (NO3− and NH4+), dissolved organic nitrogen (DON), N2 (denitrification), O2 and TCO2 were measured in the tidal reaches of the Bremer River, south east Queensland, Australia. Measurements were made at three sites during summer and winter. Fluxes of NO3− were generally directed into the sediments at rates of up to −225 μmol N m−2 h−1. NH4+ was mostly taken up by the sediments at rates of up to −52 μmol N m−2 h−1, its ultimate fate probably being denitrification. DON fluxes were not significant during winter. During summer, fluxes of DON were observed both into (−105 μmol m−2 h−1) and out of (39 μmol m−2 h−1) the sediments. Average N2 fluxes at all sampling sites were similar during summer (162 μmol N m−2 h−1) and winter (153 μmol N m−2 h−1). Denitrification was fed both by nitrification within the sediment and NO3− from the water column. Sediment respiration rates played an important role in the dynamics of nitrification and denitrification. NO3− fluxes were significantly related to TCO2 fluxes (p<0.01), with a release of NO3− from the sediment only occurring at respiration rates below 1000 μmol C m−2 h−1. Rates of denitrification increased with respiration up to TCO2 fluxes of 1000 μmol C m−2 h−1. At sediment respiration rates above 1000 μmol C m−2 h−1, denitrification rates increased less rapidly with respiration in winter and declined during summer. On a monthly basis denitrification removed about 9% of the total nitrogen and 16% of NO3− entering the Bremer River system from known point sources. This is a similar magnitude to that estimated in other tidal river systems and estuaries receiving similar nitrogen loads. During flood events the amount of NO3− denitrified dropped to about 6% of the total river NO3− load.  相似文献   

9.
We determined the range of the tidal variations in nutrient flux across the sediment–water interface and elucidated mechanisms of the flux variation in two estuarine intertidal flats (one sand, one mud) in northeastern Japan. Nutrient flux was measured using in situ light and dark chambers, which were incubated for 2 h, 2–6 times per day. Results showed that nutrient concentration in overlying water varied by tide and was also affected by sewage-treated water inflow. The nutrient fluxes responded quickly to the tidal variation in overlying water chemistry and the range of the variation in flux was as large as the seasonal-scale variation reported in previous studies. In the sand flat, salinity increase likely enhanced benthos respiration and led to increases in both O2 consumption and PO43− regeneration under low illumination, while benthic microalgae were likely to actively generate O2, uptake PO43− and suppress PO43− release under high illumination (>900 μmol photons m−2 s−1). Also in the mud flat, PO43− flux was related with O2 flux, although the range of temporal variation in PO43− flux was small. In both the flats, NH4+ flux was always governed by NH4+ concentration in the overlying water; either an increase in NH4+ uptake or a decrease in NH4+ release was observed as the NH4+ concentration rose due to inflow of river water or input of sewage-treated water. Although NO3− tended to be released in both tidal flats when low NO3− concentration seawater dominated, their relationship was likely to be weakened under conditions of low oxygen consumption and suppressed denitrification. It is likely that tidal variation in nutrient flux is governed more by the nutrient concentration than other factors, such as benthic biological processes, particularly in the case where nutrient concentration in the overlying water is relatively high and with wide amplitude.  相似文献   

10.
The isotopic composition (δ15N) of dissolved nitrate was measured at five stations within the oxygen-deficient region of the eastern tropical North Pacific Ocean (ETNP) and at one station 900 km northeast of Hawaii, which was considered to be representative of all major water masses of the Pacific. At this last station, the δ15N composition of dissolved nitrate decreased systematically from about +6‰ at 400 m to approximately +5‰ at 5,000 m; these results are consistent with other estimates from the western Pacific.In contrast, vertical profiles of δ15N of dissolved nitrate from the ETNP showed marked departure from the above observed trend and correlated with losses of nitrate arising from denitrification. Instantaneous fractionation factors (α) were estimated, using the one dimensional vertical diffusion-advection model. These results suggest that 14NO3− is consumed 3–4% faster than 15NO3−, significantly larger than fractionations (2%) observed under laboratory conditions.Maximum rates of denitrification at 100 m were also evaluated and ranged from 0.6 to 8 μg-at 1−1 yr−1 for the stations investigated. The above upper limit is probably excessive, but the average maximum for the four stations analyzed is estimated to be 3.5 μg-at NO3− 1−1 yr−1. These results compare favorably with suitably corrected oxygen utilization rates derived from electron transport activity measurements.  相似文献   

11.
Rates of transformation, recycling and burial of nitrogen and their temporal and spatial variability were investigated in deep-sea sediments of the Porcupine Abyssal Plain (PAP), NE Atlantic during eight cruises from 1996 to 2000. Benthic fluxes of ammonium (NH4) and nitrate (NO3) were measured in situ using a benthic lander. Fluxes of dissolved organic nitrogen (DON) and denitrification rates were calculated from pore water profiles of DON and NO3, respectively. Burial of nitrogen was calculated from down core profiles of nitrogen in the solid phase together with 14C-based sediment accumulation rates and dry bulk density. Average NH4 and NO3-effluxes were 7.4 ± 19 μmol m−2 d−1 (n = 7) and 52 ± 30 μmol m−2 d−1 (n = 14), respectively, during the period 1996–2000. During the same period, the DON-flux was 11 ± 5.6 μmol m−2 d−1 (n = 5) and the denitrification rate was 5.1 ± 3.0 μmol m−2 d−1 (n = 22). Temporal and spatial variations were only found in the benthic NO3 fluxes. The average burial rate was 4.6 ± 0.9 μmol m−2 d−1. On average over the sampling period, the recycling efficiency of the PON input to the sediment was 94% and the burial efficiency hence 6%. The DON flux constituted 14% of the nitrogen recycled, and it was of similar magnitude as the sum of burial and denitrification. By assuming the PAP is representative of all deep-sea areas, rates of denitrification, burial and DON efflux were extrapolated to the total area of the deep-sea floor (>2000 m) and integrated values of denitrification and burial of 8 ± 5 and 7 ± 1 Tg N year−1, respectively, were obtained. This value of total deep-sea sediment denitrification corresponds to 3–12% of the global ocean benthic denitrification. Burial in deep-sea sediments makes up at least 25% of the global ocean nitrogen burial. The integrated DON flux from the deep-sea floor is comparable in magnitude to a reported global riverine input of DON suggesting that deep-sea sediments constitute an important source of DON to the world ocean.  相似文献   

12.
Nitrogen uptake rates, and physical, chemical and biological characteristics of the euphotic zone were studied in winter, spring and late summer during the period 1992–1994 along a transect (Line P) extending from the continental slope off the southwest corner of Vancouver Island (British Columbia, Canada; station P4; 49°N, 127°W) to open waters in the NE Pacific (OSP; 50°N, 145°W). Nitrate (NO3−) and silicic acid (Si(OH)4) concentrations increased offshore during every season. Lowest NO3− and Si(OH)4 values were observed during late summer and spring, and highest during winter throughout the euphotic zone. For spring and late summer, surface depletion of NO3− was observed at the inshore end of the transect, while offshore concentrations were never limiting for phytoplankton growth. Silicic acid was never depleted at any depth or station during the period covered by this study. Ammonium (NH4+) and urea concentrations exhibited a patchy distribution along the transect, with no seasonal variations. Chlorophyll a and particulate nitrogen did not show a consistent longitudinal pattern from year to year. In general, the highest concentrations of chlorophyll a and particulate nitrogen were measured during the late summer cruises, with lower values in spring and lowest in winter. Phytoplankton assemblages were numerically dominated by flagellates <5 μm throughout the water column on each cruise transect. Ammonium, urea and NO3− uptake rates represented on average 55, 24 and 21% of the depth-integrated total nitrogen uptake, both longitudinally and seasonally; hence, phytoplankton utilized nitrogen in the following order: NH4+>urea>NO3− along Line P. Ammonium may have inhibited the uptake rates of NO3− and urea. Urea uptake rates were lower than those of NH4+, but higher values were occasionally observed at a few depths along the transect, particularly during the spring of 1993. Depth-integrated NH4+ uptake rates were generally higher inshore, while NO3− uptake rates showed higher values offshore during most seasons. In contrast, urea uptake rates did not exhibit a consistent longitudinal trend. The depth-integrated f-ratio ranged from 0.05 to 0.37 with an average of 0.21 for all stations and cruises, and was overestimated on average by 36% when urea was excluded from the calculation. On a yearly basis, primary productivity in the NE subarctic Pacific was based on regenerated nitrogen.  相似文献   

13.
浒苔对NH+4-N与NO-3-N吸收的相互作用   总被引：1，自引：0，他引：1  
在国内首次研究了大型海洋绿潮藻浒苔(Ulva prolifera)对NH4+-N与NO 3--N两种氮源的选择吸收作用。结果表明:当两种氮源等浓度比例存在时,随着NH4+-N与NO3--N浓度升高,藻体对NH4+-N的吸收速率逐渐升高,而对NO3--N吸收受到抑制;当NO3--N和NH 4+-N高浓度比存在时,藻体对NH4-N的吸收速率随着NO3--N/NH4+-N比例的升高和NH4-N浓度的下降而降低;当NO3--N和NH4+-N低浓度比存在时,藻体对NH+4-N保持较高的吸收速率,而对NO3--N的吸收效率随着NO3--N浓度的降低而降低;浒苔具有同时利用水体中较高浓度的NH+4-N和NO3--N的能力,只有当NH4+-N或NO3--N浓度较低时,才以吸收相对应的氮源为主。这说明浒苔能够快速、大量地吸收水体中氮源,为爆发性增殖贮备物质条件。同时,即便两种氮源同时存在,浒苔对NH+4-N的吸收速率也远高于对NO3--N的吸收速率,因此,控制NH4+-N的大量输入仍是预防浒苔绿潮爆发的关键。  相似文献   

14.
Egg production of Calanus finmarchicus was studied during joint basin-scale surveys in April–June 2003 in the Norwegian Sea. Surveys covered the whole Norwegian Sea and were conducted from Norwegian, Icelandic and Faroese research vessels. Stations were classified as being in pre-bloom, bloom or post-bloom phase according to levels of chlorophyll a and nitrate. Individual egg production rates and population egg production rates were calculated and compared between areas. Both individual egg production rates (eggs female−1 day−1) and population egg production rates (eggs m−2 day−1) were significantly higher in bloom areas compared with pre-bloom and post-bloom areas. However, when integrated over an estimated duration of the three phases, the time-integrated egg production (eggs m−2) in most years was highest in the pre-bloom phase, and this was explained by the longer duration of this phase compared with the two other phases.  相似文献   

15.
The microscale (1 and 4 cm sampling resolution) distributions of chemical (O2, NH3, NO3−, NO2−, PO43−) and biological (Chl a, phytoplankton, bacterioplankton, viruses) parameters were measured in the 16 cm of water immediately overlaying the sediment-water interface (SWI) within a temperate mangrove estuary in South Australia during December 2003 and March 2004. Shear velocities (u*) during the time of sampling were very low (<0.1 cm s−1), and we consequently predict that resuspension of organisms and materials was negligible. In December 2003, profiles were often characterised by strong gradients in nutrients and organisms, with the highest concentrations often observed within 0.5 cm of the SWI. Microscale patterns in O2, NH3, NO3− and NO2− indicated that a variety of anaerobic and aerobic transformation processes probably occurred at the SWI and within profiles. Strong gradients in PO43− were indicative of nutrient flux across the SWI as a consequence of degradation processes in the sediments. Pico- and nanophytoplankton concentrations were strongly correlated (p < 0.01) to PO43−, and exhibited 12- and 68-fold changes in abundance, respectively, with highest concentrations observed nearest to the SWI. Several bacterial subpopulations were discriminated using flow cytometry and significant shifts in the ‘cytometric structure’ of the bacterial community were observed within microscale profiles. Two populations of viruses were correlated to the phytoplankton and low DNA (LDNA) bacteria, and each exhibited elevated concentrations within 0.5 cm of the SWI. In March 2004, microscale distributions of O2 and nutrients were more homogenous than in December 2003, and dissimilar microbial community structure and patterns were observed above the SWI. The patterns observed here support the prediction that benthic processes can strongly influence the ecology of planktonic communities in the overlaying water, and provide further evidence for the existence of microscale variability amongst communities of aquatic microorganisms.  相似文献   

16.
Estimating nitrogen transformation rates in aquatic ecosystems by isotope dilution techniques is simplified by directly measuring nitrogen isotopic ratios for NH4+ in the water using high performance cation exchange liquid chromatography (HPLC). Modifications of HPLC conditions and implementation of a median-area method for retention time determination improved and linearized a previously reported sigmoid relationship between the retention time shift (RTshift) of the NH4+ peak and the ratio of [15NH4+]: [Total NH4+] in seawater fortified with 15NH4+. Increasing the temperature of the HPLC column from 47 to 85 °C increased mobile phase buffer flow rate relative to column back pressure, decreased the retention time for NH4+, and allowed the buffer pH to be optimized relative to the pK of NH4+. The use of median-area rather than maximum-height to define the retention time of NH4+ further improved the linearity (r > 0.995) of the relationship between the ratio [15NH4+]: [Total NH4+] and RTshift over the range of isotope ratios. Reduction of NO3− to NH4+ by adding zinc dust to acidified (pH 2) seawater or lakewater samples, followed by pH neutralization, and subsequent analysis of NH4+ isotope ratios by HPLC, extended application of the method to isotope dilution experiments with NO3−. Advantages of this direct-injection method over mass-measurement approaches traditionally used for isotope dilution experiments include small sample size and minimal sample preparation.  相似文献   

17.
Wind-driven cyclonic eddies are hypothesized to relieve nutrient stress and enhance primary production by the upward displacement of nutrient-rich deep waters into the euphotic zone. In this study, we measured nitrate (NO3−), particulate carbon (PC), particulate nitrogen (PN), their stable isotope compositions (δ15N-NO3−, δ13C-PC and δ15N-PN, respectively), and dissolved organic nitrogen (DON) within Cyclone Opal, a mature wind-driven eddy generated in the lee of the Hawaiian Islands. Sampling occurred in March 2005 as part of the multi-disciplinary E-Flux study, approximately 4–6 weeks after eddy formation. Integrated NO3− concentrations above 110 m were 4.8 times greater inside the eddy (85.8±6.4 mmol N m−2) compared to the surrounding water column (17.8±7.8 mmol N m−2). Using N-isotope derived estimates of NO3− assimilation, we estimated that 213±59 mmol m−2 of NO3− was initially injected into the upper 110 m Cyclone Opal formation, implying that NO3− was assimilated at a rate of 3.75±0.5 mmol N m−2 d−1. This injected NO3− supported 68±19% and 66±9% of the phytoplankton N demand and export production, respectively. N isotope data suggest that 32±6% of the initial NO3− remained unassimilated. Self-shading, inefficiency in the transfer of N from dissolved to particulate export, or depletion of a specific nutrient other than N may have led to a lack of complete NO3− assimilation. Using a salt budget approach, we estimate that dissolved organic nitrogen (DON) concentrations increased from eddy formation (3.8±0.4 mmol N m−2) to the time of sampling (4.0±0.09 mmol N m−2), implying that DON accumulated at rate of 0.83±1.3 mmol N m−2 d−1, and accounted for 22±15% of the injected NO3−. Interestingly, no significant increase in suspended PN and PC, or export production was observed inside Cyclone Opal relative to the surrounding water column. A simple N budget shows that if 22±15% of the injected NO3− was shunted into the DON pool, and 32±6% is unassimilated, then 46±16% of the injected NO3− remains undocumented. Alternative loss processes within the eddy include lateral exchange of injected NO3− along isopycnal surfaces, remineralization of PN at depth, as well as microzooplankton grazing. A 9-day time series within Cyclone Opal revealed a temporal depletion in δ15N-PN, implying a rapid change in the N source. A change in NO3− assimilation, or a shift from NO3− fueled growth to assimilation of a 15N-deplete N source, may be responsible for such observations.  相似文献   

18.
Global change models predict effects of climate change on hydrological regimes at the continental scale in Europe. The aim of this study was to gain a better understanding of the possible effect of changing external forcing conditions on the functioning of estuarine ecosystems. In densely populated areas, anthropogenic nutrient enrichment and consequent alteration of nutrient biogeochemical cycles have already had a big impact on these ecosystems. The average yearly discharge of the upper Schelde estuary increased nearly threefold over the period 1996–2000, from 28 m3 s−1 in 1996 to 73 m3 s−1 in 2000. The continuously rising discharge conditions over the five-year period were used as a reference situation for possible future effects of climate on ecological functioning through increase of discharge. At high discharges, nutrient (NH4+, NO3−, dissolved silica and PO43−) concentrations in the tidal fresh- and brackish water showed a decrease of up to 50% while total discharged nutrient loadings increased up to 100%. Opposite effects of increasing discharge on NH4+, NO3− and dissolved silica concentrations in summer and winter, resulted in the flattening out of seasonal cycles for these nutrients. Under high discharge conditions, silica uptake by diatom communities was lowered. Dissolved silica loadings to the coastal area increased concurrently with total silica loadings upstream. Salt intrusion to the marine parts of the estuary decreased. This resulted in a downstream shift of the salinity gradient, with lower salinity observed near the mouth. As a result, TDIN, NO3− and dissolved silica concentrations doubled at the mouth of the estuary.  相似文献   

19.
The effect of a sudden increase in salinity from 10 to 37 in porewater concentration and the benthic fluxes of ammonium, calcium and dissolved inorganic carbon were studied in sediments of a small coastal lagoon, the Albufera d'Es Grau (Minorca Island, Spain). The temporal effects of the changes in salinity were examined over 17 days using a single diffusion-reaction model and a mass-balance approach. After the salinity change, NH4+-flux to the water and Ca-flux toward sediments increased (NH4+-flux: 5000–3000 μmol m−2 d−1 in seawater and 600/250 μmol m−2 d−1 in brackish water; Ca-flux: −40/−76 meq m−2 d−1 at S=37 and −13/−10 meq m−2 d−1 at S=10); however, later NH4+-flux decreased in seawater, reaching values lower than in brackish water. In contrast, Ca-flux presented similar values in both conditions. The fluxes of dissolved inorganic carbon, which were constant at S=10 (55/45 mmol m−2 d−1), increased during the experiment at S=37 (from 30 mmol m−2 d−1 immediately after salinity increase to 60 mmol m−2 d−1 after 17 days).In brackish conditions, NH4+ and Ca2+ fluxes were consistent with a single diffusion-reaction model that assumes a zero-order reaction for NH4+ production and a first-order reaction for Ca2+ production. In seawater, this model explained the Ca-flux observed, but did not account for the high initial flux of NH4+.The mass balance for 17 days indicated a higher retention of NH4+ in porewater in the littoral station in seawater conditions (9.5 mmol m−2 at S=37 and 1.6 mmol m−2 at S=10) and a significant reduction in the water consumption at both sites (5 mmol m−2 at S=37; 35/23 mmol m−2 at S=10). In contrast, accumulation of dissolved inorganic carbon in porewater was lower in seawater incubations (−10/−1 meq m−2 at S=37; 50/90 meq m−2 at S=10) and was linked to a higher efflux of CO2 to the atmosphere, because of calcium carbonate precipitation in water (675/500 meq m−2). These results indicate that increased salinity in shallow coastal waters could play a major role in the global carbon cycle.  相似文献   

20.
A red tide due toGymnodinium nagasakiense was observed in August 1988 in Tanabe Bay, Wakayama Prefecture, Japan. The maximum cell concentration ofG. nagasakiense reached 1×105 cells ml–1 at the surface water. From May to September 1988, the following were monitored: water temperature, salinity, chlorophylla, D.O., dissolved nutrients (NO2–N, NO3–N, NH4–N, PO4–P DON, DOP), particulate nutrients (PON, POP) and three dissolved selenium species [Se(IV), Se(VI), Organic Se]. Dissolved inorganic nitrogen (NO3–N, NH4–N) decreased but PON, POP, DON, DOP and inorganic phosphate increased at the peak of the bloom. The concentration of organic selenium increased up to the bloom initiation period which started on 5 July, and then the concentration of Se(IV) increased as the concentration of organic selenium decreased at the peak of the bloom (3 August). The strong relationship was found between the concentration of Se(IV) and the cell concentration ofG. nagasakiense (r 2=0.98). The Se(IV) requirement ofG. nagasakiense was 2.89×10–17 moles cell–1, which was agreed well with 4.4×10–17 moles cell–1 found in a laboratory experiment onG. nagasakiense using selenium spiked artificial sea water medium. The average ratio of Se(IV) to dissolved inorganic nitrogen (DIN) during the red tide bloom was 11441, the ratio of Se(IV) to DIN at the surface with the maximum cell concentration ofG. nagasakiense of 1×105 cells ml–1 was 1137. These results suggested that selenium may play an important role in red tide outbreak ofG. nagasakiense.  相似文献   

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