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1.
目的：观察茵陈四苓颗粒联合骨髓间充质干细胞（BMSCs）对急性肝衰竭大鼠线粒体膜电位的调节作用。方法：将60只雄性SPF级SD大鼠随机分成A组（正常组）、B组（茵陈四苓颗粒联合骨髓间充质干细胞组）、C组（茵陈四苓颗粒组）、D组（模型组）、E组（复方甘草酸苷组）、F组（骨髓间充质干细胞组）,每组各10只。采用硫代乙酰胺腹腔注射的方法制备急性肝衰竭大鼠模型,进行BMSCs的分离纯化、培养及鉴定,观察大鼠的一般情况与存活情况,血清天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）、总胆红素（TBil）的含量,肝脏组织病理情况、肝线粒体的形态与结构、肝线粒体膜电位。结果：大鼠血清ALT、AST、TBil水平比较,D组较A组明显升高（P<0.05）,B、C、E、F组较D组降低,其中B组降低更显著,差异均有统计学意义（P<0.05）,E、F组组间差异无统计学意义（P>0.05）。B、C、E、F组肝脏组织病理、线粒体形态与结构的变化情况较D组好转。D组Q1-UR/Q1-LR阳性百分比比值显著升高,与A组比较,差异有统计学意义（P<0.05）;B、C组与D组比较,差异有统计学意义（P<0.05）;各干预组肝线粒体膜电位稳定,E组相似文献
2.
目的：研究柴胡疏肝散合济川煎对肝郁气滞合并阳虚型便秘型肠易激综合征（IBS-C）模型大鼠的治疗效果及作用机制。方法：将56只SPF级雄性SD大鼠随机分为正常组（8只）和模型组（48只）,模型组采用冰水灌胃、限制饮水结合慢性束缚应激的方法制备肝郁气滞合并阳虚型IBS-C大鼠模型。将造模成功的大鼠随机分为模型对照组、复方聚乙二醇电解质散（PEG-EP)组、黛力新组及中药低、中、高剂量组,每组各8只。各组进行药物干预14 d。观察指标包括大鼠一般状态、糖水偏好率、旷场实验评分、内脏敏感性、小肠推进率、结肠黏膜HE染色及血清5-羟色胺( 5-HT) 、生长抑素(SS) 、血管活性肠肽（VIP）含量测定。结果：与模型对照组相比,柴胡疏肝散合济川煎能使模型大鼠的粪便粒数、粪便含水量、Bristol 分值、内脏敏感性、旷场试验评分、糖水偏好百分比、小肠推进率增加(P<0.01 或P<0.05),血清5-HT、SS含量降低(P<0.05),血清VIP含量升高（P<0.05）;与PEG-EP组、黛力新组相比,中药高剂量组大鼠血清5-HT、SS均降低(P<0.05),VIP水平升高(P<0.05)。结论：大鼠肝郁气滞合并阳虚型IBS-C模型制备成功,柴胡疏肝散合济川煎可改善模型大鼠肝郁和便秘症状,其作用机制可能与降低大鼠血清5-HT、SS含量和升高大鼠血清VIP含量有关。 相似文献
3.
目的：探讨艾灸阳明经穴对变应性鼻炎（AR）大鼠行为学的改变及改善AR情感反应可能的作用环节。方法：将30只SD大鼠随机分为空白组、AR模型组及艾灸阳明经穴组（艾灸组），每组各10只，采用卵清蛋白致敏方法制作AR大鼠模型。观察大鼠鼻部症状以及躯体行为学的改变来评估情感变化；观察鼻黏膜组织病理改变；酶联免疫吸附测定法（ELISA）检测血清总免疫球蛋白E（sIgE）、白细胞介素4（IL-4）及干扰素（IFN-γ）含量；分离大鼠脑海马区组织免疫组织化学检测神经肽P物质（SP）、星形胶质细胞（GFAP）和小胶质细胞（OX-42）的蛋白表达，并进行比较。结果：与空白组比较，AR模型组大鼠搔鼻、流涕次数明显增多，行为学评分显著升高（P<0.01）；鼻黏膜上皮破坏明显，纤毛脱落，腺体增生及水肿，黏膜上皮深面固有层可见大量嗜酸性粒细胞（EOS）浸润；血清sIgE、IL-4含量升高（P<0.01）、IFN-γ含量下降（P<0.01），SP、GFAP、OX-42蛋白表达升高（P<0.01）；与AR模型组比较，艾灸组大鼠搔鼻、流涕频次明显减少，行为学评分改善（P<0.05）；鼻黏膜上皮损伤、纤毛紊乱程度、腺体增生及水肿有所改善，黏膜下EOS数量减少（P<0.05）；血清sIgE、IL-4含量显著下降（P<0.05）、IFN-γ含量上升（P<0.05），OX-42、GFAP、SP蛋白表达明显改善（P<0.05）。结论：AR组大鼠致敏后的鼻部症状及躯体行为学表现可能与鼻部致敏后激活了海马区情感行为相关的炎症介质有关，导致海马区神经胶质细胞炎症反应的发生。艾灸阳明经穴能显著改善AR情感反应，此效应可能通过下调AR大鼠脑内海马区炎性反应水平，减少神经胶质细胞过度活化而发挥作用。 相似文献
4.
目的：研究抗癌消水散对人胃癌小鼠腹腔移植阳虚模型的影响。方法：采用腹腔注射人胃癌细胞MGC803及冰水建立人胃癌小鼠腹腔移植阳虚模型，将造模成功的50只小鼠分为模型组及抗癌消水散低、中、高剂量组，每组各10只，另取10只正常小鼠为对照组。干预30 d后评价阳虚症状积分，测定血清中促肾上腺皮质激素（ACTH）、皮质醇（CORT）、三碘甲状腺原氨酸（T3）、甲状腺素（T4）、促甲状腺激素（TSH）含量及B7-1、B7-2、CD28、胞毒性T淋巴细胞相关抗原-4（CTLA-4）含量，检测腹膜组织中B7-1（CD80）、B7-2（CD86）、CD28 mRNA、CTLA-4 mRNA及转化生长因子-β1（TGF-β1）、白细胞介素-10（IL-10）含量，观察腹水量、瘤体积、瘤重。结果：与正常组比较，模型组阳虚症状积分显著增加，血清ACTH、CORT、T3、T4、TSH水平显著降低，差异均有统计学意义（P<0.01）。抗癌消水散低、中、高剂量组小鼠腹水量、瘤体积、瘤重，血清中B7-1、B7-2、CD28、CTLA-4含量，腹膜组织中B7-1、B7-2、CD28 mRNA、CTLA-4 mRNA及TGF-β1、IL-10含量与模型组比较，差异均有统计学意义（P<0.05）；上述指标低、中、高剂量组组间比较，差异亦有统计学意义（P<0.05），以高剂量组改善为最佳。结论：抗癌消水散具有抗人胃癌小鼠移植阳虚模型瘤恶性腹水的作用，这可能与抑制CTLA-4表达，促进T细胞激活，改善机体免疫抑制及腹腔免疫微环境有关。 相似文献
5.
目的：基于半夏不同炮制方法探究半夏秫米汤治疗失眠的效果，筛选出最佳疗效品种及剂量，并探究其作用机制。方法：实验分2轮进行，均采用对氯苯丙氨酸腹腔注射法复制大鼠失眠模型，后予以中药干预。第1轮实验结束后通过翻正实验筛选出最优疗效组进行第2轮实验，第2轮实验结束后检测翻正实验，同时采用酶联免疫吸附（ELISA）法检测大鼠脑组织中5-羟色胺（5-HT）、去甲肾上腺素（NE）、白介素-1β（IL-1β）的浓度。结果：第1轮筛选实验结果显示，雌雄大鼠体质量阳性组和姜半夏组与模型组比较，差异均有统计学意义（P<0.01，P<0.05）。睡眠潜伏期缩短时间及睡眠持续时间方面，雌性大鼠阳性组、姜半夏组、生半夏组与模型组比较，差异均有统计学意义（P<0.01，P<0.05），其中阳性组＞姜半夏组＞生半夏组。雄性大鼠阳性组、姜半夏组与模型组比较，差异均有统计学意义（P<0.01，P<0.05），其中阳性组＞姜半夏组。第2轮剂量实验结果显示，雌性大鼠体质量阳性组、姜高组、姜中组与模型组比较，差异均有统计学意义（P<0.01，P<0.05）。与模型组比较，阳性组、姜高组、姜中组可明显缩短大鼠睡眠潜伏期，延长睡眠持续时间，升高5-HT、IL-1β水平，降低NE水平，差异均有统计学意义（P<0.01，P<0.05），其中阳性组＞姜高组＞姜中组。结论：高剂量姜半夏组成的半夏秫米汤治疗失眠效果最优，其机制可能是通过调节下丘脑-垂体-肾上腺轴通路上神经递质的含量发挥作用。 相似文献
6.
目的：探讨失荅剌知丸及其拆方对脑缺血再灌注损伤模型大鼠的作用机制及其有效配伍成分。方法：将204只大鼠随机分为空白对照组（A组），假手术组（B组），模型组（C组），失荅剌知丸组（D组），巴豆组（E组），巴豆+三药组（F组），巴豆+余药组（G组），其中C、D、E、F、G组制备局灶性脑缺血再灌注模型，并按取材时间点不同分为1、3、7d组。各组大鼠取材前应用Garcia JH法进行神经功能评分；应用HE染色法观察大鼠各时间点脑组织病理学形态改变；免疫荧光法、Western Blot检测细胞外调节蛋白激酶1/2（ERK1/2）蛋白表达水平。结果：1）神经功能评分结果。与A、B组相比，其余各组评分均降低（P＜0.01）；与C组相比，D、E、F、G组评分均增加（P＜0.01）；各给药组中，D组评分高于E、F、G组，且E、F、G组中又以F组评分最高（P＜0.05或P＜0.01）；C、D、E、F、G组评分均随给药时间延长而增加。2）大鼠脑组织海马区HE染色结果。C组大鼠海马CA1区神经细胞较A、B组排列松散、紊乱、模糊，胞核与胞质界限欠清晰；与C组相比，D、E、F、G组神经细胞形态改善明显，且以D组改善最为明显；E、F、G组中以F组改善明显。3）大鼠脑组织海马区免疫荧光结果。C组较A、B组大鼠脑组织海马区p-ERK1/2的阳性细胞计数增加（P＜0.01）；与C组相比，D、E、F、G组p-ERK1/2阳性细胞计数升高（P＜0.01）；各给药组中，D组计数最多，E、F、G组中以F组计数最多（P＜0.05或P＜0.01）；C、D、E、F、G组p-ERK1/2的阳性细胞计数均随给药时间延长而升高。4）Western-bolt结果。与A、B组相比，其余各组大鼠脑组织海马区p-ERK1/2活化程度均升高（P＜0.01）；与C组相比，D、E、F、G组p-ERK1/2活化程度均升高（P＜0.01）；各给药组中C组p-ERK1/2活化程度均优于E、F、G组，E、F、G组中以F组p-ERK1/2的活化程度最显著（P＜0.05或P＜0.01）；C、D、E、F组p-ERK1/2活化程度均随给药时间延长而升高。结论：D、E、F、G组均可改善大鼠脑缺血再灌注后的神经功能损伤，抑制神经细胞凋亡，这可能与其参与调节MAPK-ERK通路中ERK1/2的表达相关；E、F、G组中以巴豆、柴胡、黑诃子、芦荟4味药物的组方治疗效果最佳，考虑该组药物可能是失荅剌知丸能有效抑制局灶性脑缺血再灌注损伤的主要药物组成；巴豆可能是四味药中发挥脑保护作用的主要药物之一。 相似文献
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目的：探讨心痛泰对急性心肌缺血大鼠新生微血管密度及血管内皮活性物质的影响。方法：将SD大鼠随机分成假手术组，模型组，心痛泰低、中、高剂量组，麝香保心丸组。除假手术组外，其余组大鼠均予以结扎冠状动脉左前降支制备成急性心肌缺血损伤模型，成模后分别给予蒸馏水，心痛泰低、中、高剂量药液及麝香保心丸进行灌胃治疗2周。HE染色光镜观察心肌形态结构改变；ELISA试剂盒检测血清中一氧化氮（NO）、一氧化氮合酶（NOS）、内皮素1（ET-1）含量；免疫组化法测定新生微血管密度。结果：与假手术组相比，模型组血清中NO、NOS表达减少（P<0.01），ET-1表达增加（P<0.01），缺血心肌微血管密度表达增加（P<0.05）；与模型组相比，各用药组均可升高血清中NO、NOS含量（P<0.01），降低ET-1含量（P<0.01），促进缺血区微血管密度的表达（P<0.05）。结论：心痛泰具有保护缺血心肌之效，这可能与其提高血清中NO、NOS含量、减少ET-1的含量、促进缺血区新生血管生成相关。 相似文献
8.
目的：观察背俞穴埋线法治疗月经先后无定期的临床疗效。方法：将45例月经先后无定期患者随机分为治疗组、对照1组和对照2组,每组各15例。治疗组采用背俞穴埋线治疗,对照1组采用常规腹部腧穴埋线治疗,对照组2采用背俞穴针刺治疗。3组均连续治疗4个月经周期,比较3组中医证候积分、子宫内膜厚度,评定综合疗效。结果：总有效率治疗组、对照1组、对照2组分别为93.33%（14/15）、73.33%（11/15）、66.67%（10/15）,治疗组与对照1、2组比较,差异均有统计学意义（P＜0.05）。3组中医证候积分、子宫内膜厚度治疗前后组内比较及治疗后治疗组与对照1、2组比较,差异均有统计学意义（P<0.01或P<0.05）。结论：采用背俞穴埋线法治疗月经先后无定期能更好地改善患者的中医证候积分、子宫内膜厚度,临床疗效佳。 相似文献
9.
目的：观察穴位埋线疗法治疗脾虚湿阻型产后肥胖的临床疗效。方法：将70 例产后肥胖患者随机分成2组,每组各35 例。治疗组采用穴位埋线疗法,对照组采用针刺疗法,2组取穴相同。观察治疗前后2组患者的腰围、臀围、体质量、体质量指数（BMI）的变化,评定综合疗效。结果：总有效率治疗组为100.0%（35/35）,对照组为88.6%（31/35）,差异有统计学意义（P＜0.05）;2组治疗后体质量、BMI值、腰围均较治疗前减小,差异有统计学意义（P<0.05）,且治疗组小于对照组（P<0.05）。2组臀围治疗前后组内比较及治疗后组间比较,差异均无统计学意义（P>0.05）。结论：穴位埋线对患者的体质量、腰围、BMI的改善程度明显,可获得理想的减肥效果。 相似文献
10.
目的：探讨加味丹参饮预处理通过抑瘤基因/磷脂酰肌醇-3激酶/蛋白激酶B（PTEN/PI3K/Akt）信号通路对大鼠心肌缺血再灌注损伤（IRI）的保护作用及机制。方法：将75只雄性远交群（SD）大鼠随机分为5组：假手术组、模型组、加味丹参饮组、加味丹参饮+PI3K/Akt通路抑制剂（LY294002）组、LY294002组，采用结扎大鼠冠状动脉左前降支30min后再灌注60min的方法制备IRI模型。采用酶联免疫吸附测定（ELISA）法检测各组大鼠心肌肌钙蛋白I（cTnI）表达；取心肌梗死边缘区心肌组织，采用脱氧核糖核苷酸末端转移酶介导的缺口末端标记（TUNEL）法检测心肌凋亡指数，采用免疫组化法检测各组大鼠心肌组织Akt、PTEN的表达。结果：与假手术组比较，模型组cTnI水平显著升高，大鼠心肌凋亡指数增加，PTEN、Akt表达均显著升高（P<0.05）；与模型组比较，加味丹参饮组cTnI水平均显著降低，Akt表达升高，心肌凋亡指数和PETN表达降低（P<0.05）；与加味丹参饮组比较，加入抑制剂后，cTnI水平均明显降低，Akt表达降低，PTEN表达升高（P<0.05）。结论：大鼠心肌缺血再灌注时，加味丹参饮可以通过降低PTEN表达，激活PI3K/Akt信号通路，抑制大鼠心肌细胞的凋亡，从而减轻心肌损伤，保护心肌。 相似文献
11.
The analysis of the structure of the cryolithozone, facies, and thicknesses of the Quaternary sediments and the results of the physicochemical mathematical modeling of the modern shelf of the western part of the Laptev Sea support the influence of the Late Pleistocene glaciations on the heat conditions and the distribution of the permafrost in the area. A ～200-m thick glacier formed under aerial conditions from atmospheric precipitation represented the metamorphosed snow cover. According to the modeling, the long-living (from 60?50 to 10?4 ky) glacier reduced the thickness of the permafrost rocks in the reviewed shelf area for 280–360 m. The Holocene marine transgression additionally decreased the thickness from 50–140 m on the inner shelf to 220–350 m on the outer shelf. The modern submarine cryolithozone 450-0 m thick is wide-spread in the studied region from the coast to the shelf boundary (isobaths of 130–140 m), where it pinches out at a distance of ～380 km from the coast at a depth of ～250 m above the sea level. 相似文献
12.
We analyze the components of the carbon system of the Sevastopol bay waters and the balance of main sediment-forming substances
using the data of field investigations in 1998–2008. The interannual variations of total inorganic carbon and the equilibrium
partial pressure of carbon dioxide in the bay water are noted. An increase in the flux of carbon dioxide into the bay and
in the content of organic carbon in bottom sediments is revealed, and an explanation of this phenomenon is given. The priority
accumulation of organic carbon in the sediments of the bay is established. We assess the interannual variation in the relative
abundances of organic and inorganic carbon as an index of the carbon cycle stability. 相似文献
13.
We generalize and analyze the state of investigations of the influence of river waters on the hydrologic structure of the
Black Sea. Specific features of the water, regime and hydrography of rivers flowing into the sea, data on the discharge of
rivers, and intrayear and interyear variability of the discharge are presented. We discuss and analyze works studying the
transformation of river waters, routes of desalinized sea waters, and their influence on the thermohaline structure and dynamic
regime of the sea.
Translated by Peter V. Malyshev and Dmitry V. Malyshev 相似文献
14.
V. A. Ivanov S. P. Lyubartseva N. Mikhailova N. B. Shapiro 《Physical Oceanography》2001,11(6):509-524
We analyze the results of numerical calculations performed according to the three-dimensional interdisciplinary model of an
ecological system of the Black-Sea shelf zone near the estuary of the Danube. The complete system of equations of hydrothermodynamics
is solved together with transport equations of the advection-diffusion-reaction type used to describe the transformation of
a substance (nitrogen) between the components of the characteristic vectors of the ecosystem: plankton, detritus, and biogenic
elements (nitrates). We describe the distinctive features of the circumcontinental distribution of components obtained as
a result of numerical experiments and present arguments for the conclusion that the ecosystem of the Danube estuary water
area plays the role of a buffer zone between the press of the Danubian biogenic pollutions and the neighbouring areas of the
shelf zone and open sea.
Translated by Peter V. Malyshev and Dmitry V. Malyshev 相似文献
15.
Ten years of semistationary studies of the interaction of the ice cover and the coastal relief of the Kandalakhsa Gulf in the White Sea allowed us to reveal the peculiarities of these processes in the warm and cold years and the mechanisms of the origination of ice forms, including ice tents, which govern the character of the tidal flats. Three dynamic fast ice zones are distinguished by their geomorphological location, morphology, and ice dynamics. The dependence of the mechanisms and volumes of the enrichment of the fast ice in the sediments on the coastal zone??s relief was established. At the coast of the Kindo Peninsula (Velikaya Salma Strait), the ice mostly contains fine-sandy sediments. Coarser sediments, including boulders, are occasional and frozen into the ice at the contact of the fast ice and the bottom. At the expense of the ice??s spreading by 4?C6 times in the warm years and 2?C4 times in the cold years, several thousand tons of sediments mostly from the tidal flat??s surface (100?C700 m3 from 1 km of coast in the wide tidal flats and <50 m3 on the steep coasts) are delivered to the water area. 相似文献
16.
Relationship between the path of the Kuroshio in the south of Japan and the path of the Kuroshio Extension in the east 总被引：2，自引：0，他引：2
A relationship between paths of the Kuroshio and Kuroshio Extension (KE) is investigated, using the satellite-derived altimetry
dataset of 1993–2008. When the Kuroshio takes the nearshore nonlarge meander path or typical large meander path and resultantly
goes through the deeper channel (about 2500 m) of the Izu-Ogasawara Ridge, the KE path adopts a relatively stable state with
the two quasi-stationary meanders. On the other hand, when the Kuroshio takes the offshore nonlarge meander path and then
passes over the shallower part of the Ridge (about 1000 m), the KE path tends to be convoluted, i.e., an unstable state. 相似文献
17.
I. E. Timchenko A. A. Primalenny V. D. Yarin E. F. Vasechkina E. M. Igumnova 《Physical Oceanography》2001,11(4):367-378
We use many-year archival observations of hydrologic and hydrobiologic state of the Sea of Azov for the identification of
the structure of a model of its ecosystem. The ecosystem model, supplemented with expert estimates of bioresource consumption
(fish reserve), contamination level, and possible ecologic fines for violation of the sea natural state, is formalized by
the method of system dynamics. Hereat, the major part of influence functions is found in terms of observational data with
application of self-organization algorithms. We also present the results of simulated experiments with the model of the ecosystem,
which enable us to analyse scenarios of its behaviour under the influence of various external factors (wind, river discharge,
water exchange with the Black Sea, etc.).
Translated by Peter V. Malyshev and Dmitry V. Malyshev 相似文献
18.
I. G. Ostrovskaya 《Physical Oceanography》1992,3(5):397-403
Three mathematical models are considered: a two-dimensional model for calculating the concentration fields of the ecosystem components on the shelf of the Republic of Guinea, a pointwise model for the annual variation of the components, and a two predators-prey model. These models are used to study the effect of hydrodynamic conditions on the state of the shelf ecosystem and for research into the causes which distort the balance between marketable and unmarketable fish.Translated by Mikhail M. Trufanov. 相似文献
19.
Al. A. Shreider 《Oceanology》2007,47(1):91-103
The first data on the temporal evolution of the slope of the buried depression of the Black Sea are obtained. The sharp change in the rate of the decrease in the slope steepness that occurred in the Late Miocene-Pliocene time reflects a reorientation of the vector of mutual movement and a related intensification of the collision between the Arabian and Eurasian lithospheric plates. 相似文献
20.
The study of the sedimentary body of the Bransfield Strait has made it possible to identify several sedimentary complexes, to construct the first electronic charts for the acoustic basement, and to establish four stages of the evolution of its floor, which updates the previous knowledge about the formation of the strait. At the first stage, there was an increase in tension stresses that were accompanied by the local splits of the continental crust at the periphery of the Antarctic Peninsula. At the second stage, a graben-like structure filled with the Lower stratigraphic complex was formed northward of the Antarctic Peninsula. At the third stage, the continuing processes of extension led to intensive explosive activity of the growing volcanic structures and filling of the graben with sediments of the Middle seismostratigraphic complex. The fourth stage, which has continued until recently, is characterized by quasi-linear localization of the major centers of volcanic activity in the band closer to the South-Shetland Islands and the formation of the Upper seismostratigraphic sedimentary complex. The evolution of the floor of the Bransfield Strait reflects the process of penetration of the American-Antarctic ridge to the continental lithosphere of the Antarctic Peninsula for the last million years. 相似文献