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1.
对天山乌鲁木齐河源1号冰川末端融水细菌进行分离,检测了分离菌株的生理生化表型特征,通过16S rRNA基因序列分析确定分离菌株的系统进化地位.结果表明:天山乌鲁木齐河源1号冰川融水中分离的36株代表菌株分属于5个系统发育类群、8个属,其中,Bacteroidetes和γ-Proteobacteria为优势类群,分别占41.7%和38.9%;在属水平上,假单胞菌属(Pseudomonas)菌株占39%.依据菌株最适生长温度,36株分离菌株80%以上属于耐冷菌;产酶实验显示,大多数菌株同时产蛋白酶和脂肪酶,仅3株菌不产酶,6株菌同时产脂肪酶、淀粉酶、蛋白酶.耐药性试验表明,36株分离菌株对各种抗生素有不同程度的耐药,其中,7株菌对测试的10种抗生素均具有耐药性.研究结果将有助于了解天山乌鲁木齐河源1号冰川微生物物种多样性、生理多样性,同时为评估气候变化、人类活动对冰川微生物生理特性潜在的影响提供依据. 相似文献
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利用厌氧微生物分离技术,对深度为1.2 m 的海南红树林湿地沉积物钻孔样品进行了分离培养,共获得11 株
厌氧sulfate-reducing bacteria（SRB） 菌株。经显微观察和16S rDNA序列分析,可归纳为6个属,其中已经报道有芽孢杆菌
属（Bacillus）、弧菌属（Vibrio） 和梭状芽胞杆菌属（Clostridium）,另外3个属分别为伯克霍尔德菌属（Burkholderia）、希瓦氏菌属（Shewanella） 和海杆菌属（Marinobacterium）。不同属的细菌对硫酸盐还原的速率最低为14.71%,最高可达
56.78%,并且以上6属11株菌都能将+6价的硫还原生成-2价硫,并与培养基中的Fe2+结合生成黑色FeS沉淀,而这些无定
形FeS沉淀是生成黄铁矿的前体。红树林湿地SRB种群数量随沉积物深度的增加而降低,结合沉积物的地球化学分析测试
结果表明,表层（0 cm） 水界面的沉积物由于处于氧化-还原界面,氧气的周期性输入在一定程度上抑制了SRB的生长;随着
深度增加（10~40 cm）,充足的有机质、偏中性的pH值以及厌氧环境的增强,使得SRB种类和数量明显增加;而60 cm以下
沉积物中因TOC含量降低,减少了微生物可利用的碳源,pH值明显降低,Na+和Ca2+离子浓度明显增加,这些因素都抑制了
SRB的生长,使得深部沉积物中SRB的种类和数量显著减少。 相似文献
4.
在18℃的低温条件下,从不同菌源中富集、驯化、筛选得到两株高效苯酚降解菌株A4和B14,在转速为150r·min-1、温度为18℃、pH为6～9的条件下,两株菌对苯酚起始浓度为300mg/L的苯酚降解率分别为90.43%和99.02%.在中性条件下,对苯酚起始浓度小于300mg/L的苯酚降解率均保持在98%以上.经形态特征观察及生理生化实验初步鉴定,结果显示,A4为微球菌属,B14为假单胞菌属.对菌株的降解特性研究表明两株菌最适生长的pH值为6～9,A4菌株比B14菌株具有更广泛的pH适应性;菌株对苯酚的降解率随着生物投加量的增加而升高,在投菌量大于5mL·100 mL-1时,苯酚降解率接近100%;两株菌在通气状况良好的条件下,对苯酚的降解率及其生长情况明显优于缺氧条件.通过对比实验,A4菌株对外界环境的适应性明显强于B14,而后者的生长速率明显高于前者. 相似文献
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青藏高原冻土链霉菌分离菌株多样性及其生理活性的研究 总被引：3，自引：2，他引：1
以青藏高原腹地北部地区5个钻孔中采集的冻土样品为材料, 采用高氏1号琼脂和甘油精氨酸琼脂两种培养基, 分离得到54株可培养放线菌.经形态学初步鉴定并结合16S rDNA序列分析, 将分离菌株归入链霉菌属. 抑菌实验和生理生化特性研究发现, 24%的链霉菌对供试病原菌有抑菌活性, H2S、 有机酸、 H2O2酶、 脂酶Ⅰ(Tween-20)、 脂酶Ⅱ(Tween-40)、 脂酶Ⅲ(Tween-80)、 淀粉酶、 蛋白酶、 脲酶产生菌分别占分离菌株的56%、 28%、 93%、 22%、 67%、 41%、 43%、 61%、 57%. 结果显示, 青藏高原冻土蕴藏有丰富的放线菌资源, 其次级代谢产物的丰富性和多样性, 对开发研究放线菌资源具有重要价值. 相似文献
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安徽铜陵尾矿堆中三价砷抗性菌株的分离、鉴定及16S rDNA分析 总被引：1，自引：0，他引：1
从安徽铜陵杨山冲尾矿库采集表层尾矿样品,通过砷富集培养,从3份样品中筛选得到3株具有较强As(Ⅲ) 抗性的细菌 WK-21、WK-31 和 WK-32,用不同 NaAsO2 浓度的 LB 培养基对菌株进行砷抗性检测,结果这3株菌分别能耐受32、56和50 mmol/L的As(Ⅲ),具有较高的耐砷性.对这3株菌进行不同梯度浓度 As(Ⅲ) 环境中的生长规律研究,结果表明砷在低浓度时对细菌生长有轻微促进作用或者几乎没影响,但随着砷浓度增大,抑制作用明显增强.根据对这3株菌进行形态观察、生理生化鉴定以及16S rDNA序列比对分析,鉴定出这3株菌分别属于Arthrobacter sp.、Rhodococcus equi和Paenibacillus sp.菌属.由于这些菌株分离于尾矿库的尾矿堆中,因此,本文研究结果为进一步研究尾矿库金属污染的微生物修复及微生物抗金属机制提供了材料. 相似文献
8.
青藏高原土壤中一株低温原油降解菌的分离鉴定及其原油降解特性 总被引：2，自引：2，他引：0
从青藏高原班戈桥地区土壤中分离到一株能利用原油为碳源生长的细菌(BGQ-6). 通过16S rRNA基因序列比对及Biolog GEN Ⅲ鉴定板确定该菌株为Rhodococcus qingshengii. 将生长至对数期的菌株接入MM培养基, 10 ℃、150 rpm条件下培养15 d后, 通过GC法检测到该菌对原油的总降解率为74.14%, 且对直链烷烃、支链烷烃、环烷烃和芳香烃等60种烃类有较高的降解率. 通过特异性基因扩增检测到该菌株基因组中具有4个alkB和1个almA两种烷烃羟化酶基因. 相似文献
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地下水中锰离子氧化细菌的分离与筛选鉴定 总被引：1，自引：0，他引：1
从沈阳张士经济开发区净水厂的成熟生物滤池中,分离筛选得到了3株具有较强Mn~(2+)氧化能力的革兰氏阴性细菌,分别命名为Mn1、Mn2和Mn3.经3个菌株的16S rDNA鉴定及其系统发育学分析表明,菌株Mn1和Mn2为Delftia属,菌株Mn3为Klebsiella属.进一步考察了3株菌对地下水中浓度为2 mg/L的Mn~(2+)氧化及发酵能力.结果表明,3株菌均可在12 h内达到使1 m~3生物滤料成熟时所需的最少生物量;且Mn1、Mn2和Mn3对Mn~(2+)的相对氧化能力分别为96%、94%和85%,为生物滤池法去除地下水中铁锰的快速启动提供了菌种基础. 相似文献
10.
一株产铁载体细菌的筛选及其与云母的相互作用 总被引：2，自引：0，他引：2
产铁载体细菌与云母类矿物相互作用的研究有助于了解矿物生物风化和土壤形成的演化规律和机理。采用纯培养法
自南京龙山废钾矿区酸模根际土壤分离筛选到一株高产荧光铁载体的细菌Z6,通过16S rDNA 序列分析和生理生化反应将其
鉴定为假单胞菌属（Pseudomonas sp.）;通过室温静置培养试验研究Z6 菌株与云母的相互作用结果表明,细菌生长代谢前期
（8~15 d）快速产生大量铁载体,菌株与矿物共培养75 d 后,产铁载体细菌对云母类矿物确实产生了风化作用,黑云母组发
酵液中Fe 含量增加了211 倍,Si 含量增加了约27.8 倍,SEM 显示云母表面出现溶蚀坑,细菌可以在矿物表面形成生物膜或
矿物- 细菌复合物。不同矿物对共培养体系中细菌数量、荧光铁载体含量、溶液pH 的影响不同。 相似文献
11.
Iron and phosphorus limitation and their combined effects on the growth and development of Prorocentrum micans Ehrenberg (Dinophyceae) and Cryptomonas sp. (Cryptophyceae) have been studied. The growth status of these algae at different Fe and P levels is quantitatively analyzed
through lab-culture experiments. Results show that Fe starvation can misalign chloroplast lamellas, and metamorphose and twist
thylakoid, leading to a retarded growth rate. The combined effects of Fe and P can accelerate their growth, and an optimum
culture condition to maintain the maximum growth rate is a combination of 0.05 μM Fe and 50 μM P. Algal uptake of Fe and P
are synergistic. Their cellular concentrations bear significant correlations (r = 0.9979 for Cryptomonas sp. and 0.9938 for P. micans; P < 0.01) and correspondingly the molar P:Fe ratios are ∼160:1 and ∼356:1, respectively. These findings will provide novel
insights for revealing the crucial factors controlling the occurrence of algal blooms. 相似文献
12.
A biogeochemical orientation survey was carried out in the vicinity of an epithermal Au deposit in the Moisan Au–Ag mineralized area, Haenam district in Korea. The Au–Ag bearing quartz veins of the mine occur as narrow open-space fillings within Cretaceous silicic pyroclastics. The vein minerals consist mainly of quartz, sericite, pyrite, chalcopyrite, and galena, with some electrum and argentite. The main objectives of this study were to study the geochemical characteristics of rocks, soils and plants in this area, to investigate the spatial relationship between Au and associated elements in rock–soil–plant system, and to evaluate the applicability of biogeochemical prospecting for Au vein occurrences in Korea. Samples of rocks and soils, and leaves of three plant species (Japanese red pine — P. densiflora, oriental white oak — Q. aliena, Japanese mallotus — M. japonicus) were collected from the target mineralized area and control barren locations, and analyzed for trace elements by instrumental neutron activation analysis. Sampling lines were composed of one slope line which is almost parallel to the mineralized quartz-veins, and four transect lines spaced 100 m apart across the veins at 20 m sampling intervals. From the multi-element data of rock samples (n = 9), high values of Au (maximum 2030 ppb) are spatially related to Au–quartz veins. Soil samples (n = 61) collected from five sampling lines show higher values of Au (24–825 ppb) whereas soil samples from the control locations have lower values of Au (below 25 ppb). Many plant species collected from the vicinity of the veins have high Au contents compared with those at the control locations, but the ranges of Au values are variable among plant species. In a total of 128 samples of plant leaves, Q. aliena yielded Au values of 0.4 to 6.9 ppb, and M. japonicus 0.9 to 4.1 ppb. Gold contents in P. densiflora ranged from 0.1 to 5.6 ppb. Plant leaves from control areas show less than 1.6 ppb Au. The biological absorption coefficient (BAC) of Au in plants decreases in the order of Q. aliena > M. japonicus > P. densiflora. Based on the results of the study, Q. aliena appeared to be the best sampling media for biogeochemical prospecting of Au in the study area. 相似文献
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Eco-physiological and plant performance responses and acclimation of Populus euphratica Oliv. to water release of the lower reaches of Tarim River, China were investigated. Three representative areas and 15 transects
were selected along the lower reaches of the Tarim River. The groundwater level and salt content as well as plant performance
and the contents of proline, soluble sugar, and plant endogenous hormone (ABA, CTK) in leaves were monitored and analyzed
before- and after-water release. The groundwater level was raised in different areas and transects by the water release program.
The physiological stress to P. euphratica decreased after the water release. Our results suggested that the groundwater level in the studied region changed from −3.15
to −4.12 m, salt content of the groundwater from 67.15 to 72.65 mM, the proline content from 9.28 to 11.06 mM, the soluble
sugar content from 224.71 to 252.16 mM, the ABA content from 3.59 to 5.01 ng/(g FW), and the CK content from 4.01 to 4.56 ng/(g
FW)- for the optimum growth and recover of P. euphratica indicated by the plant performance parameters, and the efficiency of water release was the highest. 相似文献
14.
C25 HBI alkenes of the diatom, Haslea ostrearia, have been examined in experiments in which the algae were cultured for up to 10 days at 14–15°C at nine different salinities (15 to 40 per mil). H. ostrearia proved to be an osmotolerant organism and growth was observed at all salinities. After 10 days growth at the lowest and highest salinities of 15 and 40 per mil, the concentrations of 2,10,14-trimethyl-6-methylene-7-(3-methylpent-1-enyl)pentadec-9-ene, which was the only HBI present in any of the samples, averaged 1.2±0.7 and 2.1±0.7 pg cell−1, respectively. These were slightly lower than the HBI concentrations at 25 to 35 per mil (2.8±0.5 pg cell−1). The data indicate that although salinity has an influence on HBI production in H. ostrearia, factors other than salinity are probably more important in controlling the large variations of HBI alkene concentrations and distributions found in sedimentary environments. 相似文献
15.
The diagnoses of the conchostracan genera Cratostracus and Porostracus are revised following a re-examination of their type species under a scanning electron microscope. In Cratostracus each growth line has a serrated lower margin. Radial lirae branch into smaller ridges that merge with each other to give the upper part of each growth band in the ventral region of the carapace a slightly undulating surface. Cross-bars are present between these lirae. In Porostracus the radial lirae on the growth bands near the umbo are long and relatively widely spaced. There is a fine reticulum between them in the umbonal part of the carapace and cross-bars on the ventral surface. The ornament of Porostracus indicates that it can be assigned to the family Halysestheriidae rather than to the Afrograptidae, in which it has been placed previously. Morphological analysis of most of the species of the two genera and closely related Orthestheria (Migransia) under the scanning electron microscope is required before their stratigraphic potential can be fully realized. 相似文献
16.
贺兰山构造带及邻区中-新生代构造事件:来自不整合面和裂变径迹的约束 总被引：1，自引：1，他引：0
贺兰山构造带及邻区的构造属性长期以来存在争议,确定该地区中新生代的构造事件及隆升过程是了解这一重要陆内变形带动力学机制的关键所在。本文采用不整合面分析法和低温热年代学方法,综合分析探讨了贺兰山构造带及邻区中新生代的构造事件及其构造演化过程。通过对该地区的野外地质调查,本次在中-新生代地层中由底到顶识别出6个不同类型的不整合面,它们分别是:(1) T_(2-3)/P平行不整合面;(2) J/AnJ角度不整合面-微角度不整合面;(3) K_1/AnK_1高角度不整合面;(4) E_3q/AnE_3;(5) N_1/AnN_1;(6) Q/An Q。在T_3d~3、J_2y和K_1变形前锋,可见与逆冲-褶皱造山带相关的同构造沉积生长地层,其在形态上表现为超覆、削截,在黄草滩等地局部与倒转背斜相伴生。这些不整合和生长地层是构造活动的直接证据。本次研究对采自该地区的12件样品分别进行了磷灰石、锆石裂变径迹测年及热史模拟分析。结果表明,裂变径迹年龄主要分布在4个区间,对应地质时代分别为中侏罗世-晚侏罗世(168～159Ma)、早白垩世末(139～91Ma)、晚白垩世末(79～66Ma)、始新世(59～50Ma),反映出该地区在这4个时期发生了明显的冷却抬升事件,且这4期构造事件与野外观察到的地质特征有很好的地质响应。同时,热史模拟表明该地区整体上经历了晚侏罗世、早白垩世、晚白垩世末-始新世3期快速隆升事件。综合研究表明,该地区主体逆冲褶皱的时间是从中侏罗世开始,早白垩世末构造运动最强烈,新生代又有所活动。 相似文献
17.
M. Aguilera C. Espinar J.P. Ferrio G. Prez J. Voltas 《Journal of Geochemical Exploration》2009,102(3):157
Carbon isotope composition (δ13C) in tree-rings has become routinely used in palaeoclimatic research for the assessment of changes in plant water availability in seasonally dry climates. However, the distribution of long tree-ring records around the world is very limited. Alternatively, the original climate signal of wood δ13C is well preserved in fossil charcoal and, accordingly, charcoal δ13C can be used to quantify past changes in water availability (e.g. precipitation). We report a case study on spatial palaeoclimate reconstruction which aims to characterize the transition between Bronze and Iron Ages, the so-called Iron Age Cold Epoch (ca. 900–300 BCE), using charcoals of Quercus ilex/coccifera from a set of 11 contemporary archaeological sites of eastern Spain. Climatic inferences were obtained after calibrating a linear model predicting seasonal precipitation from δ13C of Q. ilex wood samples obtained across a rainfall gradient. The best regression model corresponded to September–December (autumn) precipitation (Paut), in agreement with the fact that Q. ilex is able to exploit previous-year water reserves thanks to very effective water uptake. Subsequently, we estimated Paut from the δ13C of fossil charcoal to infer spatial patterns in water availability. Overall, estimated past Paut was about 19% higher (296 mm) than present-time values averaged across archaeological sites (249 mm). However, a clear geographic pattern of differences in precipitation could be observed in which the inner continental regions of eastern Spain were characterized by more humid conditions in the past, whereas the coastal strip of the Mediterranean Sea barely differed in past and present Paut values. The quite uniform distribution of archaeological sites over eastern Spain allowed development of contour maps of absolute and relative (to present) past Paut using gridded interpolation methods implemented in a GIS, highlighting the potential of this approach for reconstructing high-resolution spatial patterns of past climate. 相似文献
18.
Gui Zhen Men 《Mathematical Geology》1991,23(1):71-76
Hundreds of samples and 17 variables collected from coalfields of major coal-bearing strata over China except for Tibet and Taiwan, were used in this study. The dry, ash-free basis volatile matter (V
r) and caking index (G
(RI)) were chosen by means of correlation analysis and stepwise discriminatory analysis as major indices of a new classification. By means of the optimum section, the boundary value of the axis of ordinate (G
(RI)) and axis of abscissas (V
r) can be determined in the classification system. Thus, aV
r –G
(RI) classification scheme diagram was formed and bituminous coal was divided into nine classes. Use of correspondence analysis reduced dimensions of sample-expressive space without losing initial information. The trend on the factor surface of samples shows that the classification obtained from correspondence analysis conforms to theV
r –G
(RI) classification result and further verified the dependability of classification by two indices. At the same time, a certain relationship between the properties of a great variety of coal and their attributes can be explained. Hence, bituminous coal classification becomes more scientific, reasonable, and practical than before. 相似文献
19.
Huan-Xin Weng Ya-Chao Qin Xiang-Wei Sun Xun-Hong Chen Jing-Feng Chen 《Environmental Geology》2009,57(1):9-15
Laboratory culture experiments have been conducted to evaluate the effects of light intensity on the growth of Cryptomonas sp. (Cryptophyceae) and the discrepancy in absorption of iron and phosphorus under different light conditions. Results show
that there is an exponential correlation between algal growth rate and light intensity. The saturating and semi-saturating
light values for Cryptomonas sp. cells are 150 and 47 μmol photons m−2 s−1, respectively. More uptake of Fe, P, and other trace elements such as Zn, Mn, Co, and Mo is observed in the low light cultures,
although the algal growth rates are slow. The growth rate at 10 μmol photons m−2 s−1 is only 10% of that at 150 μmol photons m−2 s−1, whereas Fe and P uptake increases by 150 and 100%, respectively. These results suggest potential implications of differentiation
in absorption of iron and phosphorus at different light intensities for the occurrence of harmful algal blooms (HABs). The
mechanisms of light intensity regulating nutrient uptake as well as the occurrence of HABs are also discussed. 相似文献
20.
This paper deals with the taxonomic revision of the Early Cretaceous large, many-chambered planispiral planktonic foraminifera, historically assigned to the genus Globigerinelloides or alternatively assigned in the 1990s to the genera Globigerinelloides Cushman and ten Dam, Biglobigerinella Lalicker, Blowiella Krechmar and Gorbachik and Alanlordella BouDagher-Fadel. In a previous paper we demonstrated that the morphological and microstructural features used in the literature for distinguishing Blowiella from Globigerinelloides have value only at species level, and the former genus was thus invalidated (being the junior synonym). Moreover, the Late Aptian specimens assigned to Biglobigerinella by some authors, based on the presence of twin last chamber(s), are also included in Globigerinelloides because individuals sharing the same features (number of chambers, growth rate, size of umbilicus, and a finely perforate wall) may or may not possess twin last chamber(s). Meanwhile, Moullade et al. questioned the taxonomic value of Alanlordella, erected by BouDagher-Fadel to accommodate planispiral taxa possessing a macroperforate wall. All the species analysed here possess a finely perforate wall and consequently cannot be assigned to this taxon.The large species of Globigerinelloides retained here, with six or more chambers in the outer whorl, are G. algerianus Cushman and ten Dam, G. aptiensis Longoria, G. barri (Bolli, Loeblich and Tappan) and G. ferreolensis (Moullade).In the sections studied, Globigerinelloides aptiensis was first found close to the Barremian/Aptian boundary, even though this species was recorded in Spain (Rio Argos) in the mid Upper Barremian; very rare, small, seven-chambered individuals here assigned to Globigerinelloides ferreolensis are recorded in the Lower Aptian (just below and within the Selli Level, OAE1a), while a few specimens belonging to Globigerinelloides barri occur in the Globigerinelloides ferreolensis Zone (Upper Aptian). Globigerinelloides aptiensis and G. ferreolensis range up to the Ticinella bejaouaensis Zone while Globigerinelloides barri disappears at the top of the Globigerinelloides algerianus Zone; finally, Globigerinelloides algerianus obviously spans the eponymous total range zone.From an evolutionary point of view, two lineages within the many-chambered Globigerinelloides have been recognized. In the first, already known in the literature, Globigerinelloides aptiensis gave rise to G. ferreolensis, which evolved into G. algerianus; the latter in turn gave rise to Pseudoplanomalina cheniourensis as the final evolutionary member. In the second lineage Globigerinelloides barri originated from G. blowi. 相似文献