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墨西哥湾扇贝稚贝盐度适应性的研究 总被引：1，自引：0，他引：1
在室内控制条件下,观察不同海水盐度对墨西哥湾扇贝(Argopecten irradians concentricusSay)稚贝存活及生长的影响,并将168 h 50%死亡的盐度作为适宜生存临界盐度,将增长率为最佳增长率30%时所对应的盐度作为适宜生长临界盐度。结果表明:在稚贝壳高4.38 mm±0.31 mm,水温25.4~27.8℃,pH值7.8~8.2下,墨西哥湾扇贝稚贝适宜生存盐度为23.6~35.7,最适生存盐度为26.0~31.3;适宜生长盐度为23.9~35.8,最适生长盐度为28.7~31.3。在盐度变化超出最适范围时,`墨西哥湾扇贝稚贝对低盐更为敏感,高盐比低盐对稚贝存活、生长、增重的影响小。当盐度为28.7时,墨西哥湾扇贝稚贝具有最高的相对存活率(100%)、壳高日增长率(0.116 2 mm.d-1)及体重日增长率(1.95 mg.d-1)。当盐度在最适范围之外升高或降低,稚贝的存活率、壳高及体重日增长率也随着降低。 相似文献
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In order to improve production and breed new broods of bay scallopArgopecten irradians irradians, different-colored orange, purple and white lines were established by two mating methods of self-fertilization and mass spawning
at a commercial scallop hatchery in spring, 2002. And then larval growth and survival of different lines was compared to test
whether there is a relationship between shell color and growth and survival at early developmental stage. Both growth and
survival have no significant differences among different experimental larvae of self-fertilization or mass spawning. Results
are as following in the order of orange, purple and white shell stock. For the self-fertilization, growth rates of larvae
were 6.174, 6.412, and 6.599 μm/d, respectively. Survival rates of larvae at Day 3 were 74.41%, 76.86%, and 82.05%; Day 6
were 49.14%, 65.63%, and 52.79%; and Day 9 were 25.06%, 20.80%, and 26.47%, respectively. For the mass spawning, the growth
rates were 7.836, 7.941, and 7.878 μm/d, respectively. Survival rates at Day 3 were 93.05%, 91.95%, and 92.50%; Day 6 were
79.17%, 78.05%, and 82.50%; and Day 9 were 34.72%, 36.67%, and 38.33%, respectively. The absence of any relationship between
shell color and growth and survival at the larval stage may be resulted from their common genetic basis.
This work was financially supported by Project of Scientific Innovation, Chinese Academy of Sciences (ZKCX2-211). 相似文献
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ＩＮＴＲＯＤＵＣＴＩＯＮＫｎｏｗｌｅｄｇｅｏｆｇａｍｅｔｏｇｅｎｅｓｉｓｉｎｖａｌｕａｂｌｅｍａｒｉｎｅａｎｉｍａｌｓｉｓｖｅｒｙｉｍｐｏｒｔａｎｔｔｏｃｏｍｍｅｒｃｉａｌｆｉｓｈ ｅｒｙ.Ｇａｍｅｔｏｇｅｎｅｓｉｓｉｎｂｉｖａｌｖｅｓｗａｓｅｘｔｅｎｓｉｖｅｌｙｓｔｕｄｉｅｄａｔｄｉｆｆｅｒｅｎｔｌｅｖｅｌｓ (Ｄｏｈｍｅｎ ,1 983 ;ＤｏｒａｎｇｅａｎｄＬｅＰｅｎｎｅｃ ,1 989ａ,ｂ) .Ｓｃａｌｌｏｐｓａｎｄｏｔｈｅｒｍａｒｉｎｅｂｉｖａｌｖｅｓｈａｖｅｓｉｍｉｌａｒｒｅｐｒｏｄｕｃｔｉｖｅｃｙｃｌｅ… 相似文献
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In crustaceans, the male sexual differentiation and maintenance are specially regulated by androgenic gland (AG). However, little is known about the genes involved in the regulation process. RNA-Seq was performed on AG with ejaculatory duct (AG_ED) and ejaculatory duct (ED) as control in Eriocheir sinensis, one of the most important economic and fishery crabs with typically sex dimorphism. A total of 925 unigenes were identified as differentially expressed genes (DEGs) and the expression of nine genes randomly selected was confirmed by qRT-PCR. 667 unigenes were up-regulated in AG_ED, being supposed to be AG preferential genes. Among them, the full length of insulin-like androgenic gland factor (IAG) cDNA named as Es-IAG was obtained as a logo gene of AG, which together with the genes insulin-like receptor (INR), and single insulin binding domain protein (SIBD), might constitute the sex regulation pathway. Several sex related genes were identified, and their function will have to be investigated. Also, the identification of juvenile hormone epoxide hydrolase 1 (JHEH1), ecdysteroid 22-hydroxylase (DIB) and ecdysone receptor (ECR) preliminarily clarified the molecular regulation mechanism of eyestalk-AG-testis axis, which plays important roles in molting and reproduction. The results will enhance our understanding for the molecular basis of the AG involved in male sex regulation in crabs.
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We constructed and characterized a normalized cDNA library of Nannochloropsis oculata CS-179, and obtained 905 nonredundant sequences (NRSs) ranging from 431–1 756 bp in length. Among them, 496 were very similar
to nonredundant ones in the GenBank (E ≤1.0e-05), and 349 ESTs had significant hits with the clusters of eukaryotic orthologous groups (KOG). Bases G and/or C at
the third position of codons of 14 amino acid residues suggested a strong bias in the conserved domain of 362 NRSs (>60%).
We also identified the unigenes encoding phosphorus and nitrogen transporters, suggesting that N. oculata could efficiently transport and metabolize phosphorus and nitrogen, and recognized the unigenes that involved in biosynthesis
and storage of both fatty acids and polyunsaturated fatty acids (PUFAs), which will facilitate the demonstration of eicosapentaenoic
acid (EPA) biosynthesis pathway of N. oculata. In comparison with the original cDNA library, the normalized library significantly increased the efficiencies of random
sequencing and rarely expressed genes discovering, and decreased the frequency of abundant gene sequences. 相似文献
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<正>马氏珠母贝(Pinctada martensii)是我国南方养殖的一种重要经济贝类,长期以来一直是我国主要的海水育珠的主要贝类,用马氏珠母贝育珠是广东、广西和海南沿海部分地区经济发展的支柱性产业。近年来,我国海水珍珠的质量出现明显滑坡,严重地削弱了在国际市场上的竞争力。为提高海水珍珠质量和在国际市场上的竞争力,国内科技工作者已经进行了大量的研究工作,这些工作主要包括对马氏珠母贝养殖群体性状的改良、育珠技术的优化和海水珍珠形成机理的研究[1-5]。 相似文献
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《中国海洋大学学报(英文版)》2021,(4)
The dried shellfish products with rich nutrients and low-calorie content are favorite food in China, especially in coastal areas. However, the species of dried shellfish products in the market are usually unknown, as the taxonomic features were removed during the production process. This study described the application of DNA barcoding technique to the identification of 100 dried shellfish(scallop, squid, octopus and cuttlefish) products in markets. Samples were authenticated by comparing mitochondrial cytochrome oxidase subunit I(COI) gene and 16 S ribosomal RNA(16 S rRNA) gene sequences with public reference taxonomic databases. The results showed that all the 100 products can be identified at species level. Sixty four scallop adductor products were processed using the bay scallop, Argopecten irradians, and one was from Portuguese oyster, Crassostrea angulata. All the 27 squid, 2 cuttlefish and 6 octopus products were produced by the Jumbo flying squid, Dosidicus gigas. The neighbour-joining tree is in agreement with the results of DNA barcoding analysis. The 64 scallop samples formed one A. irradians cluster, leaving Sca65 clustered with the reference oyster sequence C. angulata(MH997922). All the 35 cephalopod(squid, octopus and cuttlefish) samples formed a D. gigas cluster. This investigation revealed a low variety of dried shellfish products sold on the market, and highlighted the high rate of mislabeling and species substitution. Our present work provides a practical method for tracing and authenticating shellfish products. 相似文献
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《中国海洋大学学报(英文版)》2021,(4)
Shell color is regarded as an economic trait in many breeding programs of bivalve mollusks, but the relationship between shell color and production traits remains controversial. In our breeding program of Crassostrea gigas, solid white, black, and orange shell lines were obtained, and second-generation(F2) and testcross families with segregating shell colors were constructed by crossing these three lines. These segregating families provided ideal samples for investigating the relationship between shell color and production traits in C. gigas. The growth and survival of 7-month-old oysters with different shell colors sampled within the same families were compared in seven F2 families and 13 testcrosses. In addition, the growth and survival of oysters from the three shell color lines were compared at 4 and 16 months of age. The growth and survival rates of the orange shell line were significantly lower than those of the white and black shell lines. However, no significant difference in growth between oysters with different shell colors was observed within segregating families, except the testcrosses produced by crossing orange-and white-shelled grandparents, and no significant difference in survival was observed in any family. Overall, no significant correlation was observed between shell color and production traits in C. gigas. These results suggest that shell color cannot be used as a marker to guide the selection of growth and survival traits. Thus shell color and production traits should be selected independently in oyster breeding programs. 相似文献
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华贵栉孔扇贝两种壳色群体生长和消化酶活力比较 总被引：2，自引：0，他引：2
以华贵栉孔扇贝桔黄和紫褐壳色个体为亲本建立两个壳色群体,从壳长、壳高、壳宽和体重几方面进行生长比较,并对两个群体做了淀粉酶和纤维素酶活力比较。结果表明:桔黄壳色群体的平均壳长、平均壳高、平均壳宽和平均体重显著大于紫褐壳色群体(P<0.05)。在24℃、27℃、30℃和33℃条件下,桔黄壳色群体的淀粉酶和纤维素酶活力显著高于紫褐壳色群体(P<0.05);不同壳色群体生长与其淀粉酶和纤维素酶活力有关。 相似文献