首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献，搜索用时 15 毫秒
1.
For revealing the effects of increasing of zinc oxide nanoparticles(ZnO NPs) on denitrification and denitrifying bacteria communities in estuarine sediments, the surface sediments of two typical estuaries(the Yangtze River Estuary and the Yellow River Estuary) were added with medium concentration(170 mg L~(-1)) and high concentration(1700 mg L~(-1)) of ZnO NPs for anaerobic culture in laboratory. The concentration of NO_3~-and NO_2~-, the reductase activity and denitrification rate were measured by physicochemical analysis, nir S gene abundance and denitrifying bacteria communities by molecular biological methods. The results showed that ZnO NPs inhibited NO_3~-, NO_2~-reduction process and NO_3~-, NO_2~-reductase activity, and a stronger inhibition effect resulting from the higher ZnO NPs concentration. ZnO NPs decreased nirS gene abundance and community diversity of denitrifying bacteria. In addition, the inhibition degree of ZnO NPs on the denitrification process of sediments in different estuaries was different. These results were of great significance for evaluating the potential ecological toxicity and risks of nanomaterials in estuaries.  相似文献   

2.
Silver nanoparticles(AgNPs)have been widely used in medicine and consumer products.And it enters the river in dif-ferent ways,then finally converges to the ocean through the estuary.AgNPs polution can affect NO2?and N2O production by denitri-fiers in aquatic system.The effects of AgNPs on denitrification activity,nitrogen transformation and nitrous oxide(N2O)emission were investigated in Dagu River Estuary(DRE)and Jiaozhou Bay(JZB).The results showed that the potential denitrification activity(PDA),NO3?and NO2?reduction rates decreased with an increase of AgNPs concentration in DRE and JZB.However,the N2O ac-cumulation was significantly increased at AgNPs concentrations above 5 mg kg?1in both areas,and the accumulation rate was greater in estuary than in bay(P<0.05).Moreover,the total bacterial count showed a slightly increasing trend with an increase of AgNPs concentration(P>0.05)in DRE and JZB.Importantly,the relative abundance of narG,nirS and nosZ gene in two areas decreased with the increase of AgNPs concentration,and the negative effect of AgNPs varied in order:nosZ>nirS>narG,inferring that the expression of denitrifying related genes could be significantly and differently inhibited by AgNPs addition.Thus,this study demon-strated that the inhibitory effect of AgNPs on different denitrification process,which may lead to the increase of inorganic nitrogen accumulation and N2O realease.This study provides a scientific basis for the further studies of AgNPs on the ecological impact mechanism and environmental effects of offshore sedimentary environment.  相似文献   

3.
Amplicon sequencing of functional genes is a powerful technique to explore the diversity and abundance of microbes involved in biogeochemical processes. One such key process, denitrification, is of particular importance because it can transform nitrate(NO3-) to N2 gas that is released to the atmosphere. In nitrogen limited alpine wetlands, assessing bacterial denitrification under the stress of wetland desertification is fundamental to understand nutrients, especially nitrogen cycling in alpine wetlands, and thus imperative for the maintenance of healthy alpine wetland ecosystems. We applied amplicon sequencing of the nirS gene to analyze the response of denitrifying bacterial community to alpine wetland desertification in Zoige, China. Raw reads were processed for quality, translated with frameshift correction, and a total of 95,316 nirS gene sequences were used for rarefaction analysis, and 1011 OTUs were detected and used in downstream analysis. Compared to the pristine swamp soil, edaphic parameters including water content, organic carbon, total nitrogen, total phosphorous, available nitrogen, available phosphorous and potential denitrification rate were significantly decreased in the moderately degraded meadow soil and in severely degraded sandy soil. Diversity of the soil nirS-type denitrifying bacteria communities increased along the Zoige wetland desertification, and Proteobacteria and Chloroflexi were the dominant denitrifying bacterial species. Genus Cupriavidus(formerly Wautersia), Azoarcus, Azospira, Thiothrix, and Rhizobiales were significantly(P0.05) depleted along the wetland desertification succession. Soil available phosphorous was the key determinant of the composition of the nirS gene containing denitrifying bacterial communities. The proportion of depleted taxa increased along the desertification of the Zoige wetland, suggesting that wetland desertification created specific physicochemical conditions that decreased the microhabitats for bacterial denitrifiers and the denitrification related genetic diversity.  相似文献   

4.
In this study,a novel culture medium that simulates shrimp pond conditions was established to screen nitrite-degrading isolates.The medium was supplemented with nitrite as a nitrogen source and shrimp feed as the major carbon source,to achieve the high nitrogen and low carbon nutritional status found in shrimp farming ponds.Screening using this medium identified potent denitrifying Bacillus isolates,among which Bacillus subtilis M7-1 was considered best.M7-1 was able to completely degrade nitrite-N in 24 h without much consumption of dissolved oxygen.Efficient denitrification activity took place in liquid cultures within a set of non-stringent ranges of pH(5.0–9.0),salinity(0–30)and temperature(25–35℃).The denitrifying enzyme gene was amplified,sequenced and further identified as nirS type.In biosecurity assessments,M7-1 had no negative effects on shrimps at a dose of 106 cfu mL−1.M7-1 could therefore be used in aquaculture to reduce and control the nitrogen concentration,and to promote the development of sustainable and healthy culture systems.  相似文献   

5.
The microbes associated with sponges play important roles in the nitrogen cycle of the coral reefs ecosystem, e.g., nitrification, denitrification, and nitrogen fixation. However, the whole nitrogen-cycling network has remained incomplete in any individual sponge holobiont. In this study, 454 pyrosequencing of the 16 S rRNA genes revealed that the sponge Spheciospongia vesparium from the South China Sea has a unique bacterial community(including 12 bacterial phyla), dominated particularly by the genus Shewanella(order Alteromonadales). A total of 10 functional genes, nifH, amo A, nar G, napA, nirK, nor B, nos Z, ureC, nrfA,and glt B, were detected in the microbiome of the sponge S. vesparium by gene-targeted analysis, revealing an almost complete nitrogen-cycling network in this sponge. Particularly, bacterial urea utilization and the whole denitrification pathway were highlighted.MEGAN analysis suggests that Proteobacteria(e.g., Shewanella) and Bacteroidetes(e.g., Bizionia) are probably involved in the nitrogen cycle in the sponge S. vesparium.  相似文献   

6.
The wide application of antibiotics in aquaculture requires an efficient treatment of the wastewater before discharging it into the environment. During the wastewater treatment, the influence of antibiotics on the performance of bioreactor should be well revealed due to their toxicity to the functional microbial community. In this study, the effect of feeding 10-30 mg L~(-1) sulfamethoxazole(SMX) in influent on the performance of an anoxic/oxic-moving bed biofilm reactor(A/O-MBBR) treating mariculture wastewater and the responding change of biofilm microbial communities was investigated. The COD average removal rate remained at 94.61%-97.34% with the dosage of SMX. Compared with that, the nitrifying removals of NH_4~+-N and NO_2~--N were violently inhibited by 30 mg L~(-1) SMX and denitrifying removal of the NO_3~--N decreased obviously with 20 mg L~(-1) or more SMX. The microbial community in the successful startup bioreactor was relatively abundant, while the diversity of microbial community decreased with the increase of feeding SMX. The salt-tolerant and SMX-resistant genera Arcobacter, Thiothrix, Desulfuromusa and Nitrosomonas were gradually enriched and finally played a vital role in converting COD and recycling nitrogen and sulfur. Hence, the present A/O-MBBR reactor with the salt-tolerant functional microbiota achieved efficient removal of pollutants in the presence of low concentration(e.g., 10 mg L~(-1)) SMX.  相似文献   

7.
At present, the understanding of the dynamics of denitrifiers at different dissolved oxygen (DO) layers under organic carbon consumption within the surface sediments remains inadequate. In this study, high-throughput sequencing and quantitative PCR targeting nirS gene were used to analyze the denitrifier abundance dynamics, community composition, and structure for aerobic (DO 0.5–6.9 mg/L), hypoxic-anoxic (DO 0–0.5 mg/L), and anoxic (DO 0 mg/L) layers in surface sediments under organic carbon consumption. Based on the analysis of nirS gene abundance, the values of denitrifying bacteria decreased with organic carbon consumption at different DO layers. When the bacterial species abundance at the genus level were compared between the high-carbon and low-carbon sediments, there was significant increase in 6 out of 36, 7 out of 36 and 6 out of 36 genera respectively for the aerobic, hypoxic-anoxic and anoxic layers. On the other hand, 14 out of 36, 9 out of 36 and 15 out of 36 genera showed significant decrease in bacterial species abundance respectively for the aerobic, hypoxic-anoxic and anoxic layers. Additionally, 14 out of 36, 20 out of 36, and 15 out of 36 genera had no change in bacterial species abundance respectively for the aerobic, hypoxic-anoxic, and anoxic layers. This indicates that the carbon utilization ability of different denitrifiers on each DO layers was generally different from each other. Diversity of denitrifying bacteria also presented significant differences in different DO layers between the high- and low-carbon content sediment layers. Moreover, under the high-carbon and low-carbon content, the abundance of nirS gene showed a high peak within the hypoxic-anoxic regions, suggesting that this region might be the main distribution area for the denitrifying bacteria within the surface sediments. Furthermore, community of unique denitrifiers occurred in different DO layers and the adaptive changes of the denitrifier community followed the organic carbon consumption.  相似文献   

8.
本文探讨了K_2S_2O_8氧化离子色谱法测定有机氮的实验条件,考察了淋洗剂离子价态及淋洗剂总离子强度对低浓度NO_3~-与高浓度SO_4~2-分离的影响;测定了11种含氮有机化台物有机氮的回收率。本法测定地下水中有机氮的结果与经典蒸馏法结果较为一致,具有操作简便、快速、准确,效率高的特点。  相似文献   

9.
The 3-D ECOMSED ocean model was applied to establish a time-dependent boundary model for Jiaozhou Bay (JZB), in which the operator-splitting technique was used and the ‘dry and wet’ method was introduced. The influence caused by JZB reclamation on the surface level, residual currents, tidal system and tidal energy of M2 tidal system were predicted and analyzed. The results show that JZB reclamation has slight impact on the M2 tidal system, in which the variation of amplitude and phase is less than 1%.The ch...  相似文献   

10.
The variations of the meltwater runoff draining from Kartamak Glacier in Mt. Muztag Ata in China were studied by using the measured hydrological data from 1 June to 25 August 2003. The meltwater runoff is mainly affected by ambient temperature and precipitation. Meltwater and precipitation samples were collected from 10 to 23 August 2003. Their pH, EC (electric conductivity) and the major ions (Na~ , K~ , Ca~(2 ), Mg~(2 ), Cl~-, NO_3~-, SO_~4~(2-)) were determined. pH values showed a positive correlation with EC values for all samples. Meltwater samples were slightly alkaline. Sulfate and calcium were the dominant anion and cation in the measured ions, respectively. All the ion concentrations had inverse relationships with runoff or water level. In order to discuss the origins of dissolved chemical substances in the glacial meltwater, a principal component analysis was carried out. The results showed that water-rock interaction determined the ion components of the meltwater.  相似文献   

11.
The 3-D ECOMSED ocean model was applied to establish a time-dependent boundary model for Jiaozhou Bay (JZB), in which the operator-splitting technique was used and the ‘dry and wet’ method was introduced. The influence caused by JZB reclamation on the surface level, residual currents, tidal system and tidal energy of M2 tidal system were predicted and analyzed. The results show that JZB reclamation has slight impact on the M2 tidal system, in which the variation of amplitude and phase is less than 1%.The changes of the currents and residual currents in Qian Bay and near the reclamation areas are greater, but in other areas the changes are smaller, in which the currents have a change of around 1%, while the residual currents change ranges from 1.82%–9.61%. After reclamation, the tidal energy fluxes increase by 2.62%–5.24% inside and outside the JZB mouth, but decrease by 20.21%–87.23% near Qian Bay and the reclamation area.  相似文献   

12.
In this study, we used Landsat images and meteorological data to examine the spatiotemporal distribution and variability of sea ice in Jiaozhou Bay(JZB) between 1986 and 2016. The results show that JZB is not always covered by sea ice in winter, but in some extreme cases, sea ice has covered more than one-third of the sea area of the bay. Sea ice in JZB has generally formed between January 1 and February 5, primarily along the coast, and gradually expanding to the central area of the bay. Both meteorological and artificial factors have played important roles in modulating the sea ice distribution. We found sea ice coverage to have been strongly correlated with the accumulated freezing-degree days nine days before the occurrence of sea ice(R2 = 0.767). North-northwest surface winds have dominated the freezing period of sea water in the JZB, and wind speed has exerted a more significant influence on the formation of sea ice when the sea ice coverage has been generally small. Additionally, artificial factors began to affect the expansion of sea ice in JZB since 2007. The construction of the Jiao-Zhou-Bay Bridge(JZBB) is believed to have retarded water flow and reduced the tidal prism, thereby leading to the formation of an ice bridge along the JZBB, which effectively prevents the southward expansion of sea ice.  相似文献   

13.
  相似文献   

14.
In view of increasing lead pollution(Pb~(2+)) of coastal waters, the compensatory abilities of holothurians need to be assessed. The goal of the work is to clarify the functional and phenotypical differences between two types of phagocytes(P1 and P2) in Eupentacta fraudatrix exposed to Pb(NO_3)_2. It has been shown that 2 mg L~(-1) lead exposure for 48 h increases the number of P2 phagocytes as compared to P1 cells, does not significantly affect cell viability in both P1 and P2 phagocyte fractions, and significantly enhances chromatin condensation in P2 but not in P1 phagocytes. A lead concentration of 4 mg L~(-1) increases the number of P1 phagocytes compared to that of P2 type, and does not change cell viability and chromatin condensation in P1 phagocytes. In the P2 type, it decreases cell viability and does not influence the level of apoptosis. The protection against lead-induced apoptosis is apparently mediated by the activities of antioxidant enzymes, especially glutathione S-transferase. The differences in labeling cell surface receptors of P1 and P2 phagocytes by plant lectins also indicate the specific phenotypic properties of these cells. The results clarify the potential and GSH-dependent mechanisms of immune adaptation in holothurians that have been shortly exposed to lead at concentrations close to the maximum environmentally relevant level in coastal waters. Additionally, P1 and P2 phagocytes are first shown to have different functions and phenotypes during the response to lead, which indicates the complexity of the phagocytic system in holothurians and contributes to understanding the immunity evolution.  相似文献   

15.
Huguangyan Maar Lake is a typical maar lake in the southeast of China.It is well preserved and not disturbed by anthropogenic activities.In this study,microbial community structures in sediment and water samples from Huguangyan Maar Lake were investigated using a high-throughput sequencing method.We found significant differences between the microbial community compositions of the water and the sediment.The sediment samples contained more diverse Bacteria and Archaea than did the water samples.Actinobacteria,Betaproteobacteria,Cyanobacteria,and Deltaproteobacteria predominated in the water samples while Deltaproteobacteria,Anaerolineae,Nitrospira,and Dehalococcoidia were the major bacterial groups in the sediment.As for Archaea,Woesearchaeota(DHVEG-6),unclassified Archaea,and Deep Sea Euryarchaeotic Group were detected at higher abundances in the water,whereas the Miscellaneous Crenarchaeotic Group,Thermoplasmata,and Methanomicrobia were significantly more abundant in the sediment.Interactions between Bacteria and Archaea were common in both the water column and the sediment.The concentrations of major nutrients(NO_3~-,PO_4~(3-),SiO_3~(2-)and NH_4~+)shaped the microbial population structures in the water.At the higher phylogenetic levels including phylum and class,many of the dominant groups were those that were also abundant in other lakes;however,novel microbial populations(unclassified)were often seen at the lower phylogenetic levels.Our study lays a foundation for examining microbial biogeochemical cycling in sequestered lakes or reservoirs.  相似文献   

16.
Raindrop size,rainfall intensity and runoff discharge affect the detachment and transportation of soil particles.Among these three factors,the rainfall intensity seems to be more important because it can change other two factors.Storm patterns can be determined by changing the rainfall intensity during the storm.Therefore,the objective of this research is to test the influence of storm pattern on runoff,soil erosion and sediment concentration on a rangeland soil slope under field rainfall simulation.Four storm rainfall intensity patterns were selected for examining the effects of variations in storm event characteristics on soil erosion processes.The selected storm patterns were:I(45,55 and 70 mm h-1);II(45,70 and 55 mm h-1);III:(70,55 and 45 mm h-1);and IV(55,45 and 70 mm h1).The last pattern is a new one instead of the uniform pattern which has been sufficiently studied in previous researches.The experiments were conducted in field plots(in Kojour watershed,Mazandaran Province,Iran)with an area of one square meter and an constant slope gradient of 18%,surrounded by galvanised sheets.Following the nonuniform prioritization of the storm patterns for the studied variables,time to runoff(I>II>IV>III),runoff volume(III>IV>II>I),sediment concentration(IV>III>I>II)and soil erosion(III>IV>II>I)),it can be generally inferred that each pattern has specific effect on soil erosion processes during a storm.The results of the general linear model(GLM)test indicated that the effects of storm pattern on time to runoff,total runoff volume,runoff coefficient and soil erosion were significant at a level of 99%.The Duncan test showed that the storm patterns can be divided into three groups of III,IV;II;I(for time to runoff),I,II;IV,III(for runoff coefficient),and I;II;IV,III(for runoff volume and soil erosion).  相似文献   

17.
The uncertainty in assessing the numerous atmospheric pollutants transported via wind from arid and semi-arid regions is affecting the glacial ecosystem. In our study area due to the complexity of the system, a prominent seasonal difference noticed among major ions(Ca~(2+), Mg~(2+), SO_4~(2-), and NO_3~-). There is a need for understanding the ions cycling as a whole and the directionality of the feedback loops in the system. Therefore, we provide an appraisal of our current hypothesis for seasonal difference in major ion concentration from snow samples for two corresponding years(2013 and 2015) at Dokriani Glacier. A systematic study of chemical compositionsin the shallow snow pit from Dokriani Glacier was undertaken for the pre-monsoon season to understand the cycling of major ions from atmosphere to solute acquisition process. The intimating connections of ions cycling in snow and its temporal behavior was observed and analyzed through various statistical tests. Among major ions, the SO_4~(2-)has the highest concentration among anions on an average considered as 14.21% in 2013 and 29.46% in 2015. On the other side Ca~(2+) is the dominant cation contributing 28.22% in 2013 and 15.3% in 2015 on average. The average ratio of Na+/Cl-was higher in 2013 whereas lower in 2015. The backward trajectory analysis suggests the possible sources of the ions transported from Central Asia through the Western Disturbance(WD) as a prominent source of winter precipitation mainly in the Central Himalaya. Ionicconcentration of Ca~(2+) in cations was highly dominated while in anion SO_4~(2-)played the major role. Factor analysis and correlation matrix suggested that, the precipitation chemistry is mostly influenced by anthropogenic, crustal, and sea salt sources over the studied region. The elemental cycling through ocean, atmosphere and biosphere opens up new ways to understand the geochemical processes operating at the glacierized catchments of the Himalaya. Moreover, increasing the field-based studies in the coming decades would also have the certain advantage in overcoming the conceptual and computational geochemical modelling difficulties.  相似文献   

18.
Based on the layer by layer(Lb L)assembly technology,the nano-scale composite membrane with excellent structure can be prepared by changing the polyelectrolyte and controlling the deposition conditions.Polyamines and phosphate ions(Pi)can be self-organized to form supramolecular systems which could be exploited to stabilize the interfacial architecture.The LbL membrane was made of the positively charged poly(allylamine hydrochloride)(PAH)self-organized with Pi and the negatively charged poly(sodium 4-styrene sulfonate)(PSS)alternatively on top of a poly(acrylonitrile)(PAN)substrate.Compared to the membrane without Pi,the membrane assembled by PAH/Pi improved forward of flux and reduced flux of salt.The concentration of PAH and PSS,deposition time,pH and number of layers showed significant influences on the performance of the membrane.In this experiment,we systematically investigated the preparation conditions and under the optimized conditions the prepared membrane exhibited high water fluxes of 13.5 L m~(-2) h~(-1) with corresponding salt to water flux,Js/Jv,ratio of 0.07 g L~(-1) tested by forward osmosis when DI water as feed solution and 2 mol L~(-1) MgCl_2 as draw solution.  相似文献   

19.
以2015~2019年12景ALOS-2 PALSAR2影像和2018~2019年38景Sentinel-1A影像为主要数据源,利用PS-InSAR和SBAS-InSAR技术提取西藏江达县波罗乡白格滑坡点的形变信息,并对处理结果进行交叉验证。研究得到以下结论：1）PS-InSAR技术条件下,ALOS-2数据和Sentinel-1A数据的平均形变速率范围为-68.9~37.9 mm/a和-64.5~24.2 mm/a;SBAS-InSAR技术条件下,ALOS-2数据和Sentinel-1A数据的平均形变速率范围为-84.2~-40.0 mm/a和-84.0~-13.0 mm/a。2）对2种数据结果中提取的4个特征点进行时序分析和定量分析显示,2种InSAR技术结果变化趋势较为一致,验证了两者在滑坡监测中的可靠性和准确性。  相似文献   

20.
根据2003年7月台风发生期间工厂化虾池生态系中的实测数据,重点分析了不同养成密度虾池生态系中COD的变化状况及其影响因素。结果表明:台风气候对虾池生态系中COD的影响较大,台风发生时COD含量相对较高,台风前后相对较低;台风气候对COD周日变化的影响以养成密度大的养殖池较为显著,养成密度小的养殖池则影响较小;COD与环境因子之间的相关性差异也较大,台风前,养成密度大的养殖池与NO2-呈显著正相关(r=0.668),与T和Chl.a呈负相关关系(r=-0.608或r=-0.633);台风后与PO4-P、NH4+和Chl.a之间则表现的显著正相关(r=0.907、0.832、0.846)。而养成密度低的养殖池,台风前COD与pH呈显著负相关(r=-0.732),台风后与PO4-P和NO2-分别呈显著正相关(r=0.720)和良好正相关(r=0.591)。但在台风发生时,二池均无此类相关性出现。  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号