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RNA helicases of the DEAD-box and related families are involved in various cellular processes including DNA replication,DNA repair,and RNA processing.However,the function of DEAD-box proteins in aquaculture species is poorly understood at molecular level.We obtained the full-length cDNA sequences of two genes encoding helicase-related proteins,Fc-vasa and Fc-PL10a,from the testes of Chinese shrimp,Fenneropenaeus chinensis.The two predicted amino acid sequences contain all the conserved motifs characterized ... 相似文献
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A muscle cDNA library of Chinese shrimp (Fenneropenaeus chinensis) was constructed with the SMARTTM cDNA Li- brary Construction Kit. The titer of optimal primary library was 7.7×105 pfu mL-1 and that of the amplified library was 3.0×109 pfu mL-1. The percentages of the recombinant clones of primary and amplified libraries were over 98%. The insert sizes were longer than 400 bp with an average of 1000 bp. A positive clone containing a 794 bp insert was sequenced and identified encoding fast skeletal troponin Ⅰ gene. This library provided a useful resource for the functional genomic research ofE chinensis. 相似文献
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The complete mitochondrial cytochrome oxidase subunit Ⅱ (COⅡ) gene of Penaeinae shrimp Fenneropenaeus chinensis was cloned and sequenced. The gene is 688 bp in length and codes for 229 amino acids. It shows 83.2%, 87.0% and 83.8% sequence similarity to Marsupenaeus Japonicus, Penaeus monodon and Farfantepenaeus notialis, respectively. The A+T content of the whole gene and that at the third position of codons are 64.7% and 78.2%, respectively. The phylogenetic relationship between F. chinensis and three other species representing genera Farfanatepenaeus, Marsupenaeus and Penaeus was analyzed. Results showed that the genetic distances among the four taxa ranged from 0.144 0 to 0.200 5, exceeding those estimated with COⅠ and partial 16S rRNA gene sequences among Marsupenaeus, Litopenaeus and Melicertus, and being therefore larger than the value among subgenera. It has been suggested that the COⅡ gene has a faster evolutionary rate than that of the COⅠ gene and partial 16S rRNA gene and could be used for phylogenetic analysis at genus or species level. The results of the present study indicated that Farfantepenaeus, Fenneropenaeus, Marsupenaeus and Penaeus are at a higher phylogenetic level than subgenus, which supports the opinion of the elevation of phylogenetic status of the four subgenera to genus level. 相似文献
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抗菌肽对凡纳滨对虾生长和机体免疫的影响 总被引：1，自引：0，他引：1
基础饲料(对照组)中添加10g/kg抗菌肽制剂喂养体重0.10±0.01g、体长2.17±0.11cm的凡纳滨对虾(Penaeus vannamei)30d,进行10d的水浴攻毒(鳗弧菌10~8/mL),探讨饲料中添加抗菌肽对凡纳滨虾生长、成活率、免疫保护率,以及肌肉中溶菌酶活性、超氧化物歧化酶活性、总抗氧化能力的影响。结果表明:饲料中添加抗菌肽可显著提高凡纳滨对虾相对增重率、成活率和对致病菌的免疫保护率(P<0.05),但对饲料系数无显著影响(P>0.05);未受外界微生物刺激时,饲料中添加抗菌肽对凡纳滨对虾总抗氧化能力有促进作用(P<0.05),受外界微生物刺激后,饲料中添加抗菌肽喂养凡纳滨对虾可提高对虾超氧化物歧化酶活性(P<0.05)和总抗氧化能力(P<0.01)。因此饲料中添加抗菌肽喂养凡纳滨对虾可提高其机体非特异性免疫。 相似文献
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Acting as one of the pattern recognition receptors (PRRs), C-type lectin is believed to mediate pathogen recognition and plays an important role in the clearance of pathogens as part of the innate immune system. In this work, a novel C-type lectin gene (named LvLec1) was cloned from the shrimp Litopenaeus vannamei. The ORF of LvLec1 is 510 bp, encoding 169 amino acids. The deduced amino acid sequence contains a putative signal peptide of 19 amino acids at the N-terminal and a carbohydrate recognition domain... 相似文献
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Samee Haider 《中国海洋大学学报(英文版)》2010,9(2):178-184
In order to better understand shrimp allergen,some basic characters of the major allergen of greasy-back shrimp (Metapenaeus ensis)were investigated.The major allergen was extracted and separated,and its peptide mass fingerprint(PMF) was analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS).After screening in the NCBI database with Mascot searching engine,the results indicated that the major allergen of greasy-back shirmp was muscle tropomyosin.Database matching search showed that the top protein matched,i.e.the tropomyosin from giant tiger prawn(Penaeus monodon),had a Mowse value of 268.In addition,there were 27 queries matched with the allergen in greasy-back shirmp with an amino acid sequence coverage value of 65%.The matching scores and the sequence coverage values were also high with tropomyosins of other invertebrates,including Tyrophagus putrescentiae and Lepisma saccharina.These results indicated that the allergen of Metapenaeus ensis had high homology with other crustacean allergens,and provided molecular explanations for the high cross-reactivity of the major allergens between crustaceans and some other invertebrates. 相似文献
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Penaeus chinensis were reared in fibreglass tanks for the study of their selenium requirements. The shrimp were fed semipurified diets containing graded levels of selenium, and weight gains, activities of glutathione peroxidase (GSH-Px) and selenium contents in muscle and hepatopancreas were determined. Weight gain and GSH-Px activity were the highest when the shrimp were fed diet containing 20 mg/kg selenium; Good linear correlation was found between GSH-Px activities and selenium contents in the diets, and the number of healthy shrimp. The experiment showed that 20 mg/kg selenium in the diet is optimal for the shrimp and that GSH-Px activity can be an important biochemical index of the selenium nutrition status of the animal. 相似文献
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YU Shanshan KONG Xiaoyu LI Yulong and XU Hui Key Laboratory of Mariculture of Ministry of Education Ocean University of China Qingdao P. R. China 《中国海洋大学学报(英文版)》2007,(2)
The complete mitochondrial cytochrome oxidase subunit II (COII) gene of Penaeinae shrimp Fenneropenaeus chinen- sis was cloned and sequenced. The gene is 688 bp in length and codes for 229 amino acids. It shows 83.2%, 87.0% and 83.8% sequence similarity to Marsupenaeus japonicus, Penaeus monodon and Farfantepenaeus notialis, respectively. The A T content of the whole gene and that at the third position of codons are 64.7% and 78.2%, respectively. The phylogenetic relationship between F. chinensis and three other species representing genera Farfanatepenaeus, Marsupenaeus and Penaeus was analyzed. Results showed that the genetic distances among the four taxa ranged from 0.144 0 to 0.200 5, exceeding those estimated with COI and partial 16S rRNA gene sequences among Marsupenaeus, Litopenaeus and Melicertus, and being therefore larger than the value among subgenera. It has been suggested that the COII gene has a faster evolutionary rate than that of the COI gene and partial 16S rRNA gene and could be used for phylogenetic analysis at genus or species level. The results of the present study indicated that Farfantepenaeus, Fenneropenaeus, Marsupenaeus and Penaeus are at a higher phylogenetic level than subgenus, which supports the opinion of the elevation of phylogenetic status of the four subgenera to genus level. 相似文献
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REQUIREMENTS OF SHRIMP, PENAEUS CHINENSIS O''''SBECK FOR POTASSIUM, SODIUM, MAGNESIUM AND IODINE 总被引：2，自引：0，他引：2
Potassium, sodium, magnesium and iodine requirements of shrimp, Penaeus chinensis were studied.Orthogonal design was employed in this experiment. The composition of the basal diet consisted of fishmeal, peanut cake, corn meal, soybean cake, wheat bran, vitamin mix and mineral mix, and supple-mentations of potassium, sodium, magnesium and iodine in the basal diet were made according to theL_9(3~4) orthogonal table. The results indicatal that iodine supplementation improved growth of the shrimpsignificantly and raised survival very significanly, iodine requirement of the shrimp was 0.003%, sodiumrequirement was 0.87% or less; and that 1.1-1.3% potassium and 0.18-0.38% magnesium in the dietwere proper nutrition supplements for the shrimp. 相似文献
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GH-IGF-I轴是鱼体体内一个重要的内分泌生理轴,主要调控鱼体的生长发育。用RT-PCR方法从加州鲈脑垂体和肝脏组织中分别扩增出加州鲈GH和IGF-I cDNA,克隆到pMD19 T-Vector上进行序列测定和分析。结果表明:1)加州鲈GH cDNA开放阅读框长为615 bp,编码204个氨基酸,其中信号肽17个氨基酸,成熟肽187个氨基酸。成熟肽中有四个保守的半胱氨酸残基(分别位于69,177,193,202),可形成两对二硫键。加州鲈GH氨基酸序列与蓝太阳鱼、斜带石斑鱼、金头鲷、虹鳟、鲤鱼、斑马鱼相比较,同源性分别为100%、97%、94%、66%、56%、53%。2)加州鲈IGF-I cDNA开放阅读框长为561bp,编码包括信号肽和B、C、A、D、E五个区域的186个氨基酸,形成成熟肽时,信号肽和E区域被切除。成熟蛋白的氨基酸序列与GenBank中已知的河鲈、三角鲂、金头鲷、舌齿鲈、斑马鱼的相比较,结果发现四个区域的保守性有差异,A区和B区保守性较高,C区和D区保守性较差。加州鲈GH和IGF-I cDNA的获得为进一步研究鱼体GH-IGF-I轴对生长发育的调控机制奠定了基础。 相似文献
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During late February to mid-March, 1991, when mature N. japonica swarmed in the sea surface off Jimo County, Qingdao, seawater containing numerous early embryos was pumped into a nearby 2.7 ha. muddy-sand shrimp pond, where the embryos continued to develop. 800000 Penaeus chinensis seedlings were introduced into the pond on May 30, 1991. Monthly benthic samplings were carried out to determine the population dynamics and production of N. japonica in this atypical environ -ment.The density of N. japonica varied from 0 to 20400 ind./m2. The predation of P. chinensis was considered to be responsible for the mass mortality of N. japonica in June through July, when the worms were 2.2-3.3cm in length and lived in the top 2-4cm sediment. From August to September of 1991, the hottest period in the site, there was no substantial mortality of N. japonica as reported in earlier literature. This was explained by the deeper burrowing of the' worms that protected them from predation by shrimps.Monthly biomass ranged f 相似文献
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五种鲤科鱼类生长激素cDNA的克隆和序列分析 总被引：1，自引：0，他引：1
通过RT-PCR方法,以草鱼、鳙鱼、鲫鱼、鲤鱼、齐口裂腹鱼等5种鲤科重要经济鱼类的垂体总RNA为模板扩增出其生长激素(fish growth hormone,fGH)的完整ORF序列,并克隆到pMD18-T载体上,命名为pMD-1(草鱼)、pMD-2(鳙鱼)、pMD-3(鲫鱼)、pMD-4(鲤鱼)、pMD-5(齐口裂腹鱼)。测序结果显示,ORF序列其长度均为633 bp。序列分析表明,所有ORF序列均以ATG为起始密码,以TAG为终止密码,编码210个氨基酸残基,推导的生长激素前体由22个氨基酸的信号肽和188个氨基酸的成熟肽组成。同源性分析表明,草鱼、鳙鱼、鲤鱼、鲫鱼和齐口裂腹鱼的fGH同源性在93.3%~99.5%之间。 相似文献
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Chinese shrimp(Fenneropenaeus chinensis)is an economically important aquaculture species in China.However,cytogenetic and genomic data is limited in the organism partly because the chromosomes are difficult to isolate and analyze.In this study,fluorescence in-situ hybridization(FISH) was used to identify the chromosomes of F.chinensis.The 5S ribosomal RNA gene(rDNA)of F. chinensis was isolated,cloned and then used as a hybridization probe.The results show that the 5S rDNA was located on one pair of homologous chromosomes in F.chinensis.In addition,triploid shrimp were used to evaluate the feasibility of chromosome identification using FISH and to validate the method.It was confirmed that 5S rDNA can be used as a chromosome-specific probe for chromosome identification in F.chinensis.The successful application of FISH in F.chinensis shows that chromosome-specific probes can be developed and this finding will facilitate further research on the chromosomes of penaeid shrimps. 相似文献
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To study the changes of volatile compounds and antioxidant activity of fast fermented shrimp head paste during the fermentation processing,Penaeus vannamei boone head was used as the raw material,and compound starter was added for fermen-tation.During the 14 days of fermentation,the changes of free amino acids,volatile flavor compounds and main components were determined by SPME-GC-MS and electronic nose.The oxidation-resistance changes of water extract at different fermentation stages were evaluated.The results showed that the total free amino acids increased from original 2320 to 5640 mg(100 mL)?1.SPME-GC-MS analysis found that aldehydes,pyrazine compounds increased from 10.80%,1.94%to 24.35%,13.63%respectively during the fermentation process.The HO·scavenging ability of shrimp head paste increased from 52%to 86%.Our results showed that the shrimp paste produced from shrimp head fermentation could obtain good flavor and possess certain antioxidant activity of aquatic fermented condiment. 相似文献
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在建立斑节对虾实验室养殖模式的基础上，对野外采集的有白斑综合症病毒（WSSV）病典型症状的斑节对虾初步纯化其病毒，并进行WSSV的PCR检测，得到WSSV的感染用样品。对健康斑节对虾分别进行浸浴感染，投喂感染和注射感染。对感染死亡个体进行WSSA的PCR检测和细菌检测，证实WSSV感染性和致死性。浸浴感染、投喂感染和注射感染的感染量分别为4mL/L、0.2g/10g虾体、1/2稀释液0.05mL/10g虾体，死亡开始时间分别为16d、42h、28h，三种感染模式最终死亡率100%，从开始死亡到全部死亡延续时间分别为15d、82h、44h。 相似文献
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《中国海洋湖沼学报》2016,(5)
The composition and compound-specific isotopes of fatty acids were studied within food webs in the East China Sea.Lipid-normalized stable carbon isotopes of total organic carbon had a good correlation with trophic level.Variations in fatty acid compositions among different species were observed but were unclear.Different dietary structures could be traced from molecular isotopes of selected fatty acids in the Shiba shrimp(Matapenaeus joyneri),the coastal mud shrimp(Solenocera crassicomis) and the northern Maoxia shrimp(Acetes chinensis).Both M.joyneri and S.crassicomis are mainly benthos feeders,while A.chinensis is a pelagic species,although they have a similar fatty acid composition.There was a good correlation for isotopes of arachidonic acid(C20:4n6;ARA) and docosahexaenoic acid(C22:6n3;DHA)among pelagic species from higher trophic levels.The isotopic compositions of DHA in benthic species were more negative than those of pelagic species at the same trophic level.The fact that the diet of benthic species contains more degraded items,the carbon isotopes of which are derived from a large biochemical fraction,may be the reason for this variation.A comparative study of benthic and pelagic species demonstrated the different carbon sources in potential food items and the presence of a more complex system at the watersediment interface. 相似文献