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1.
Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding. 相似文献
2.
A wide-field time-series CCD photometric survey of variable stars in the field of the open cluster NGC 2168 was carried out using the BATC Schmidt telescope. In total 13 new variable stars are discovered with three W UMa systems, one EA type and two EB type eclipsing binaries (one of them could be a W UMa system), and seven pulsating stars including three candidates of δScuti stars. 相似文献
3.
Starfish oocytes with intact germinal vesicles (GVs) were cut along desired planes with glass needles or ligated using silk
thread loops into two parts and allowed to mature in vitro, and inseminated. The experimental results showed that (1) only
the parts with GVs or partial GV contents (PGVCs) cleaved, those without any GV materials did not; but nucleated and non-nucleated
fragments cut from mature eggs were able to divide; (2) the development of animal parts of oocytes containing GVs or PGVCs
was like that of animal fragments of matured oocytes with female pronuclei; most of them gave rise to permanent blastulae,
and just a few formed ectodermal vesicles with a little primary mesenchyme; (3) a large part of vegetal fragments with GVs
or PGVCs, and the vegetal parts of mature eggs without female pronuclei developed into small but normal embryos; (4) the fragments
containing GVs or PGVCs obtained from the oocytes along a plane parallel to the animal-vegetal (A-V) axis developed as normally
as the halves (with or without female pronuclei) severed from mature eggs along the same axis. Based on the data above, it
was concluded that (1) the non-chromatin materials in the oocyte GVs are indispensable for successful fertilization and cleavage
of starfish eggs; (2) some factor (s) located asymmetrically in the vegetal hemispheres of starfish oocytes is (are) responsible
for formation of the archenteron and primary mesenchyme. It is evident from the above findings that the oocyte cytoplasm of
the starfish had already regionalized before the GV break-down.
Contribution No. 1722 from the Institute of Oceanology, Academia Sinica 相似文献
4.
Baolin Tan Haisheng Ji Guangli Huang Tuanhui Zhou Qiwu Song Yu Huang 《Solar physics》2006,239(1-2):137-148
Using one-minute cadence vector magnetograms from Big Bear Solar Observatory (BBSO), we analyze the temporal behavior of derived
longitudinal electric currents associated with two flares on July 26, 2002. One of the events is an M1.0 flare which occurred
in active region NOAA 10044, while the other is an M8.7 flare in the adjacent region 10039. Rapid changes of magnetic fields
in the form of flux emergence are found to be associated with both of these events. However, the temporal behavior of electric
currents are very different. For the M1.0 flare, the longitudinal electric current density drops rapidly near the flaring
neutral line; while for the M8.7 flare, the current density rapidly increases, confirming the picture of the current-carrying
flux emergence. We offer a possible explanation for such a difference: magnetic reconnection at different heights for the
two events, near the photosphere for the M1.0 flare, and higher up for the M8.7 flare. 相似文献
5.
Recent Doppler velocity measurements have revealed the existence of two planets orbiting the star HD 12661 on medium-eccentricity orbits. The inner planet has a period of 263.6 d and a mass of 2.3 Jupiter masses, and the outer planet has a period of 1444.5d and a mass of 1.57 Jupiter masses. The stability of this system requires the two planets to be in a state of mean-motion orbit resonances. By numerical method we have studied the orbit migration and stability of the system in its early ages under the action of the proto-stellar disk, and calculated the probabilities of the planets being captured into the mean -motion resonances during their migrations. It is found that at present the two planets are probably situated at the edge of the 11:2 mean-motion resonance and are in chaotic motions. This result may be helpful to clarify the arguments on the present configuration. Besides, it is indicated that very probably, after the formation of the system, the gaseous disk has almost disappeared before the planets migrated to the present configuration. 相似文献
6.
重庆地面最高气温与最大风速年极值的渐近分布 总被引:3,自引:0,他引:3
利用重庆1951-1990年间地面最高气温和1956-1990年间地面最大风速年极值的记录,采用韦伯分布和耿贝尔分布分别对其渐近分布进行拟合.通过统计推断,找出重庆地面最高气温和地面最大风速年极值遵循的最佳渐近分布--韦伯分布. 相似文献
7.
8.
9.
蛤蜊科3种贝类16SrRNA基因片段及ITS2核苷酸序列分析 总被引:4,自引:0,他引:4
利用PCR技术分别扩增连云港及启东沿海蛤蜊科的西施舌(Coelomactra antiquata)、中国蛤蜊(Mactra chinensis)和四角蛤蜊(Mactra veneriformis)3种双壳贝的16SrRNA基因片段和ITS2核苷酸序列.测序后用DNAstar软件分析了核苷酸差异。结果显示:三种贝类16SrRNA基因片段长度相同,均为306bp(去除引物).核苷酸存在多态性。共有45个变异位点,54个核苷酸发生了变异。全部为碱基置换。西施舌与中国蛤蜊此片段核苷酸的同源性为88.9%.与四角蛤蜊的同源性为88.6%.中国蛤蜊与四角蛤蜊的同源性为90.6%。三种蛤蜊ITS2序列分别为390bp(西施舌)、441bp(四角蛤蜊)和466bp(中国蛤蜊)。存在长度多态性.ITS2核苷酸差异分析结果显示.西施舌与中国蛤蜊的同源性为70.9%-71.1%,西施舌与四角蛤蜊的为70.5%-71.0%。中国蛤蜊与四角蛤蜊的同源性为88.1%-88.8%。ITS2序列分析结果与16SrRNA基因片段分析结果一致.2种分子分析法均显示中国蛤蜊与四角蛤蜊的亲缘关系近。 相似文献
10.
The time evolution behaviour of the superhumps of the dwarf nova1RXS J232953.9+ 062814 is investigated with the wavelet analysis
method. On the basis of two nights CCD photometry performed during its first superoutburst as well as other published brightness
data, we reveal the superhump's time-dependence as a function of periods and time. Our light curves, which phased in the rapid
decay ending portion of the superoutburst and in the dawn of a following normal outburst, are important to help trace the
superhump evolution for the star. Both the superhump period and the orbital period of the binary system are detected in the
present data. We obtain P
sh=0.0458±0.0002d and P
orb=0.0450±0.0002d. They agree with those existing values. The two periods exchanged their roles during the superhump evolution.
The general profile of brightness fading over the outbursts roughly followed an exponential decay law or a form of a five-order
polynomial.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献