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HPLC法同步检测鲤鱼、对虾中喹乙醇与MQCA残留   总被引:1,自引:0,他引:1  
建立了一种高效液相色谱法同时检测鲤鱼、对虾肌肉组织中喹乙醇(OLA)及其代谢物3-甲基-喹啉-2-羧酸(MQCA)的残留。采用0.3 mol/L 盐酸提取鲤鱼、对虾中的残留物, C18 固相萃取柱净化, 氮气吹干及流动相定容后, Waters2695 高效液相色谱仪在320 nm(MQCA)和372 nm(QLA)双波...  相似文献   
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本文以人工配合饲料为对照组,4种添加剂为实验组,对中国龙虾进行50d的生长试验,结果表明:复合脱壳素(实验组1^#)效果最佳,其次分别是脱壳素(实验组2^#)、甲壳粉(实验组3^#)、喹乙醇(实验组4^#)和对照组,其增重率分别为:17.08、8.41%、7.62%、5.45%和4.21%;蜕壳率分别为58.33%、50.33%、33.33%、33.33%和33.33%;蜕壳后增重率为29.29%、16.90%、22.85%、16.43%和12.68%;饲料系数分别为7.57、7.11、7.92、11.00和14.21;对照组和实验组4^#存活率为91.67%,其余均为100%。整个试验过程龙虾生长良好。  相似文献   
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Olaquindox(OLA), one of quinoxaline-N, N-dioxides, has been put under ban. However it was used as a medicinal feed additive early; it promotes the growth of livestock and prevents them from dysentery and bacterial enteritis. In this study, we evaluated the effect of dietary OLA on the growth of large yellow croaker(Pseudosciaena crocea R.) and the histological distribution of OLA and its metabolite 3-methyl-quinoxaline-2-carboxylic acid(MQCA) in fish tissues. Four diets containing 0(control), 42.5, 89.5 and 277.2 mg kg^-1 OLA, respectively, were formulated and tested, 3 cages(1.0 m × 1.0 m × 1.5 m) each diet and 100 juveniles(9.75 ± 0.35 g) each cage. The fish were fed to satiation twice a day at 05:00 am and 17:00 pm for 8 weeks. The survival rate of fish fed the diet containing 42.5 and 89.5 mg kg^-1 OLA was significantly higher than that of fish fed the diet containing 0 and 277.2 mg kg^-1 OLA(P 0.05), while the weight gain rate of fish fed the diet containing 42.5 and 89.5 mg kg^-1 OLA was significantly higher than that of fish fed the diet without OLA(control)(P0.05), but similar to that of fish fed the diet with 277.2 mg kg^-1 OLA. Fish fed the diet with 277.2 mg kg^-1 OLA had the highest content of OLA and MQCA in liver(3.44 and 0.39 mg kg^-1, respectively), skin(0.46 and 0.09 mg kg^-1, respectively) and muscle(0.24 and 0.06 mg kg^-1, respectively). In average, fish fed the diet containing OLA had the highest content of OLA and MQCA in liver which was followed by skin and muscle(P〈 0.05), whereas OLA and MQCA were not detectable in control. Our findings demonstrated that OLA and MQCA accumulated in large yellow croaker when it was fed with the diet containing OLA, thus imposing a potential safety risk to human health.  相似文献   
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