强光照和黑暗条件下刺参体壁的基因表达差异     

张立斌  冯其明  孙丽娜  房燕  徐冬雪  张涛  杨红生 《海洋学报(英文版)》2018,37(5):54-66

参对光照变化非常敏感，研究刺参对光照的分子响应非常重要。本研究应用RNA测序获取了刺参暴露于强光（“强光”）、正常光照（“对照”）和完全黑暗（“黑暗”）环境下体壁的基因表达谱情况，通过“对照”与“黑暗”，“对照”与“强光”和“黑暗”与“强光”的比较，在|log₂ ratio|≥1和FDR≤0.001的标准下分别发现了1161、113和1705个差异表达基因（DEGs）。基因本体分析表明，“cellular process”和“binding”在“生物过程”和“分子功能”类别中的DEGs富集最多，而“cell”和“cell part”在“细胞组分”类别中的DEGs富集最多。将DEGs与Kyoto Encyclopedia基因和基因组数据库上的于214、41和229条通路进行比对，发现了51、2和57条通路分别显著富集。本研究发现的光特异性DEGs可作为深入研究刺参对光照变化的生化适应机制的重要目标基因。  相似文献   

Complete nuclear ribosomal DNA sequence analyses of the black-footed abalone Haliotis iris     

Zhansheng Guo  Leng Han 《新西兰海洋与淡水研究杂志》2018,52(2):232-246

The black-footed abalone Haliotis iris is an economically important shellfish species in New Zealand. We successfully amplified, sequenced and analysed the complete nuclear ribosomal DNA (nrDNA) of H. iris. The length of the nrDNA was determined to be around 9.6?kb and included, in order, small subunit ribosomal RNA (nrSSU, 1858bp), internal transcribed spacer (ITS, 749?bp), large subunit ribosomal RNA (nrLSU, 3412bp) and an intergenic spacer (IGS, 3560–3662?bp). The nrLSU genes were identical in two individuals, whereas the nrSSU and ITS regions existed at three and four base differences, respectively. The IGS was more variable than the other nrDNA regions. A phylogenetic tree was constructed based on the ITS sequence datasets, which revealed that Haliotidae has two major subclades, mainly distributed in the North Pacific, Europe and Australia. The complete nrDNA sequence will be useful for the classification, phylogeny and breeding of this shellfish.  相似文献   

Isotope hydrological study of mean transit time in the granitic Strengbach catchment (Vosges massif,France): application of the FlowPC model with modified input function     

D. Viville  B. Ladouche  T. Bariac 《水文研究》2006,20(8):1737-1751

Measurements of ¹⁸O concentrations in precipitation, soil solution, spring and runoff are used to determine water transit time in the small granitic Strengbach catchment (0·8 km²; 883–1146 m above sea level) located in the Vosges Mountains of northeastern France. Water transit times were calculated by applying the exponential, exponential piston and dispersion models of the FlowPC program to isotopic input (rainfall) and output (spring and stream water) data sets during the period 1989–95. The input function of the model was modified compared with the former version of the model and estimated by a deterministic approach based on a simplified hydrological balance. The fit between observed and calculated output data showed marked improvements compared with results obtained using the initial version of the model. An exponential piston version of the model applied to spring water indicates a 38·5 month mean transit time, which suggests that the volume in the aquifer, expressed in water depth, is 2·4 m. A considerable thickness (>45 m) of fractured bedrock may be involved for such a volume of water to be stored in the aquifer. Copyright © 2005 John Wiley & Sons, Ltd.  相似文献   

Isotopic evidence for holocene climatic changes in Bosten Lake, Southern Xinjiang, China     

Zhong Wei  Xiong Heigang 《中国地理科学(英文版)》1997,8(2):176-182

Based on the δ¹³C and δ¹⁸O fluctuation of lacustrine carbonate, CaCO₃ content and sporo-pollen data, a palaeoclimatic history of Bosten Lake during the Holocene has been outlined, several stages of climatic changes are divided, and the following result es are obtained: (1) Palaeoclimatic changes revealed by carbonate isotope around Bosten Lake are basically identical with that revealed by other geological records in Xinjiang. Environmental changes presented apparent Westlies Style model: during cold period, relative humidity increased, δ¹⁸O, δ¹³C and CaCO₃ appeared low; but in warm periods, the dry regime aggravated. (2) The temperature reflected by δ¹⁸O exist evident features being increase in the late period during the Holocene. Together with the δ¹³C, pollen and CaCO₃ analyses, several cold and warm phases which are of broad regional significance can be identified. The warm peaks occurred at about 11.0 ka B.P., 9.4 ka B.P., 7.5 ka B.P., 5.0 ka B.P., 3.0 ka B.P. and 2.0 ka B.P.; the cold peaks at 11.5 ka B.P., 10.5 ka B.P., 8.8 ka B.P., 5.5 ka B.P., 3.3 ka B.P., 2.2 ka B.P. and 1.5 ka B.P.. (3) Several climatic events with the nature of “abrupt climatic changes” are revealed in the periods of 11.0 ka B.P. −10.5 ka B.P., 9.4 ka B.P. −8.8 ka B.P., 5.5 ka B.P. −5.0 ka B.P. and 2.0 ka B.P. −1.5 ka B.P.. (4) The results show that carbonate isotopic record of lacustrine sediment in arid area is very sensitive to climatic changes, and may be play a very important role in understanding the features and mechanism of palaeoclimatic changes.  相似文献   

基于J2EE架构的企业卡资源管理系统的设计与实现     

任建豪  李成忠 《成都信息工程学院学报》2005,20(5):566-570

介绍了J2EE架构和多层体系结构的发展，在B／S三层架构的基础上给出了包含应用服务器中间件的多层分布式应用体系结构，并把此体系结构应用于电信氽业的有价卡管理系统项目中。分析了此项同的功能结构和体系结构，采用J2EE开发平台和技术设计实现了由浏览器、Web服务器、应用服务器、数据库服务器组成的多层体系架构。  相似文献   

蛤蜊科3种贝类16SrRNA基因片段及ITS2核苷酸序列分析   总被引：4，自引：0，他引：4  

孟学平高如承  董志国阎斌伦程汉良  李艳杰陈建安 《广东海洋大学学报》2006,26(4):8-13

利用PCR技术分别扩增连云港及启东沿海蛤蜊科的西施舌（Coelomactra antiquata）、中国蛤蜊（Mactra chinensis）和四角蛤蜊（Mactra veneriformis）3种双壳贝的16SrRNA基因片段和ITS2核苷酸序列．测序后用DNAstar软件分析了核苷酸差异。结果显示：三种贝类16SrRNA基因片段长度相同，均为306bp（去除引物）．核苷酸存在多态性。共有45个变异位点，54个核苷酸发生了变异。全部为碱基置换。西施舌与中国蛤蜊此片段核苷酸的同源性为88．9％．与四角蛤蜊的同源性为88．6％．中国蛤蜊与四角蛤蜊的同源性为90．6％。三种蛤蜊ITS2序列分别为390bp（西施舌）、441bp（四角蛤蜊）和466bp（中国蛤蜊）。存在长度多态性．ITS2核苷酸差异分析结果显示．西施舌与中国蛤蜊的同源性为70．9％-71．1％，西施舌与四角蛤蜊的为70．5％-71．0％。中国蛤蜊与四角蛤蜊的同源性为88．1％-88．8％。ITS2序列分析结果与16SrRNA基因片段分析结果一致．2种分子分析法均显示中国蛤蜊与四角蛤蜊的亲缘关系近。  相似文献   

REMOTE SENSING FOREST BIOMASS: AN EVALUATION USING HIGH RESOLUTION REMOTE SENSOR DATA AND LOBLOLLY PINE PLOTS     

John R. Jensen  Michael E. Hodgson 《The Professional geographer》1985,37(1):46-56

Eight-year-old Loblolly pine (Pinus taeda) plots located in South Carolina were evaluated using high resolution aircraft multispectral scanner data to determine if biomass could be accurately measured. Sixteen pine plots located on both sandy and clay soils were treated with 0, 180, or 360 kg. (approximately 0, 400, or 800 lb.) of nitrogen per plot. Indices of biomass from remote sensing data were significantly correlated with in situ biomass measurements made in each plot. A ratio of infrared (.9–1.1 μm) and red (.65–.70 μm) channels yielded the best correlation. The indices were not sensitive to differences in soil type (sandy or clay).  相似文献   

数字宜昌建设初探     

魏明果 《地理信息世界》2002,(3)

数字地球、数字城市是近几年刚提出来的一个全新的概念 ,但数字城市的建设却以空前的速度向前发展着 ,国务院总理朱熔基在视察宜昌时提出要把宜昌建设成世界级的城市 ,未来的城市数字化程度将是衡量一个城市发达程度的重要指标 ,启动数字宜昌建设是跻身世界级城市 ,提高宜昌市综合竞争力的重要举措  相似文献   

测绘项目管理实施引论     

龚强 《地理信息世界》2002,(4)

根据我国测绘生产单位的实际情况 ,测绘项目管理的具体实施大致包括对项目的识别、选定、启动与筹集资金、计划、成本估算、执行计划、控制、收尾等主要步骤。当项目确定后 ,需要启动和筹集资金。而计划是项目管理的基本组成部分 ,包括明确项目目标和工作范围。项目成本估算有因素估算法和利用WBS方法的详细估算。项目质量管理规划的依据有质量目标、范围说明、标准和规范等。  相似文献   

Some thoughts on pulsation and binarity     

Christiaan Sterken 《Astrophysics and Space Science》2006,304(1-4):139-143

Intensive monitoring of main-sequence stars for pulsational studies continuously reveals new binaries and multiple systems. The resulting extensive data sets lead to exact orbital parameters, and to accurate masses of the components. This is also the case higher up the main sequence, though less frequently. We discuss a few examples where intensive observational efforts are necessary in order to improve our knowledge of the pulsational behaviour in the upper main-sequence instability domains, but we emphasize that observational efforts alone that turn out more and more of the same type of data, are by far insufficient, and that there is urgent need for theoretical developments. Determination of highly accurate stellar parameters is of paramount importance and is much more needed than time-series observations.  相似文献