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Rossella Baldacconi Frine Cardone Caterina Longo Maria Mercurio Carlotta Nonnis Marzano Elda Gaino Giuseppe Corriero 《Marine Ecology》2010,31(2):309-317
The present study focuses on the reproductive success of transplants of the bath sponge Spongia officinalis Linnaeus, 1759, with the aim of investigating the possibility of restocking this species, one of the most endangered organisms of the Mediterranean sessile zoobenthos. Transplants of S. officinalis, collected from a wild population along the Apulian coasts (Ionian Sea, Italy), have been moved into an area where the species was present in the past. The transplants consisted both of specimens in toto and of fragments of different sizes, obtained after having cut the mother sponge into pieces. All transplanted sponges showed complete cicatrisation of the cut surfaces within a month of the initial manipulation and had a survival rate of 100% throughout the 12 months of the study. From the present investigation, it has emerged that the reproductive effort and the larval release by the transplants do not differ significantly from those shown by the source population. This successful technical approach supports its application as a strategy for restocking the population of this endangered species. 相似文献
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建立巴戟天多糖、还原糖含量的测定方法,优选巴戟天最佳盐蒸工艺。方法：采用苯酚-硫酸法测定巴戟天中多糖含量,采用3,5-二硝基水杨酸（DNS）法测定巴戟天中还原糖含量,并进行方法学考察;以盐蒸巴戟天色泽、质、味等方面的性状评分和多糖、还原糖及总糖含量为指标,采用拉丁方试验优选巴戟天盐蒸工艺,通过GraphPad Prism 5.0软件分析工艺参数。结果：苯酚-硫酸法测定巴戟天多糖精密度、重复性、稳定性的RSD分别为0.69%、3.00%、2.39%,加样回收率为98.39%。DNS法测定巴戟天还原糖精密度、重复性、稳定性的RSD分别为0.35%、3.10%、2.85%,加样回收率为98.85%。拉丁方试验优选巴戟天盐蒸工艺最佳加盐量、闷润时间、蒸制时间分别为3、4、9 h。巴戟天盐蒸后多糖、还原糖及总糖含量均有增加。结论：通过苯酚-硫酸法、DNS法测定巴戟天中多糖及还原糖含量,获得了最佳的巴戟天盐蒸工艺参数。 相似文献
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目的：基于网络药理学技术挖掘及佐证巴戟天-牛膝药对切合骨关节炎（OA）治则的效应机制。方法：在TCMSP数据库中筛选巴戟天-牛膝药对的所有成分,借助Uniprot数据库将药对中显效物质成分所靶向关联的效用点进行筛选,对OA的疾病靶点进行联合检索、收集与分析。将筛选出的显效靶标与OA靶点进行交联分析,将其共有靶标作为巴戟天-牛膝药对治疗OA的预测靶基因。将预测靶基因通过蛋白质-蛋白质互作关系（PPI）网络显示,筛选核心基因,构建“药物-成分-疾病-靶点”网络。联合基因本体（GO）功能和京都基因与基因组百科全书（KEGG）通路富集分析,探究巴戟天-牛膝药对切合OA治则的药效基础及效应机制。结果：经筛选分析得出槲皮素可能在巴戟天-牛膝药对治疗OA中发挥主导作用,观察预测靶基因之间的PPI关系,发现网络中转录因子AP-1（JUN）、丝氨酸/苏氨酸蛋白激酶（AKT1）、丝裂原活化蛋白激酶1（MAPK1）、V-Rel网状内皮增生病毒癌基因同源物A（RELA）、白细胞介素-6（IL-6）5个核心基因度值较高。GO功能和KEGG通路富集分析提示巴戟天-牛膝药对治疗OA主要涉及抗炎、抗氧化以及白细胞介素-17（IL-17）、肿瘤坏死因子（TNF）信号通路、细胞凋亡途径。在大鼠体外实验中,模型组与正常组比较,RELA、IL-17、IL-6蛋白表达升高（P<0.01）;经过槲皮素治疗后RELA、IL-17、IL-6蛋白表达降低（P<0.01）。结论：巴戟天-牛膝药对治疗OA的潜在机制是通过槲皮素降低RELA、IL-17、IL-6蛋白表达,抑制炎症反应,从而延缓关节软骨退变。 相似文献
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