CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES     

郭华荣  张士璀  李红岩  童裳亮  相建海 《中国海洋湖沼学报》2002,20(3):226-231

Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro.  相似文献   

南海卡氏豆荚软珊瑚化学成分研究Ⅱ     

万一千  刘青 《热带海洋学报》1997,(3)

运用光谱分析和GC－MS方法首次从卡氏豆荚软珊瑚Lobophytumcatelai中分离和鉴定出8个化合物：l，十六碳酸；2,异丁基十六碳酸酯；3，鲨肝醇；4十六酸丙酯；5，2，3一二羟基丙基十八酸酯；6，2β，3β-环氧基-5α-雄甾烷-17-醇；7，14-甲基-5α-胆甾-8-稀-3-酮；8，2α，3α一环氧基-5α-雄甾烷-17-醇。  相似文献   

Non-equilibrium alcohol flooding model for immiscible phase remediation: 1. Equation development     

Stanley Reitsma  Bernard H. Kueper 《Advances in water resources》1998,21(8):1

The equations for a compositional model for simulation of a two-phase, three-component system with inter-phase mass transfer are developed. Emphasis is placed on development of inter-phase mass transfer equations for incorporation of rate-limited inter-phase mass transfer. Due to the nature of the three-component systems considered, a single-film model may be inadequate and a two-film model must be utilized. A two-film model accounts for the simultaneous transfer of components in both directions across phase interfaces. The effect of interaction between components on diffusion is considered using a general form of Fick's Law. A Hand Plot representation of ternary phase behavior is chosen since it allows for straightforward calculation of miscibility of bulk phases under conditions of local non-equilibrium. The developed set of equations form the basis for a numerical model to simulate the enhanced removal of non-aqueous phase liquids (NAPLs) from porous media using single-component alcohol floods.  相似文献   

Facile Conversion of Alcohols to Olefins by Tosylation and Subsequent SiO2- promoted β-elimination     

RUAN Xiaohong  SONG Gaopeng  ZHANG Yichun  LI Yingxia 《中国海洋大学学报(英文版)》2007,6(2)

A convenient and effective procedure was developed for the conversion of alcohol to olefin by tosylation and subsequent β-elimination promoted by silica gel in this study. Treatment of the alcohols with p-toluenesulfonyl chloride in pyridine at 0℃ affords tosylates which undergo β-elimination with silica gel in dichloromethane or chloroform at room temperature, yielding olefinswith high productivity.  相似文献   

Cloning and Characterization of Glyceraldehyde-3-phosphate Dehydrogenase Encoding Gene in Gracilaria/Gracilariopsis lemaneiformis     

REN Xueying SUI Zhenghong ZHANG Xuecheng 《中国海洋大学学报(英文版)》2006,5(2):146-150

Glyceraldehyde-3-phosphate dehydrogenase （GAPDH） plays important roles in various cellular processes. A cytosolic GAPDH encoding gene （gpd） of Gracilaria/Gracilariopsis lemaneiformis was cloned and characterized. Deduced amino acid sequence of the enzyme of G. lemaneiformis had high homology with those of seven red algae. The 5＇-untranslated regions of the GAPDHs encoding genes of these red algae varied greatly. GAPDHs of these red algae shared the highly conserved glyceraldehyde 3-phosphate dehydrogenase active site ASCTTNCL. However, such active site of Cyanidium caldarium was different from those of the other six algae at the last two residues （CL to LF）, thus the spatial structure of its GAPDH active center may be different from those of the other six. Phylogenetic analysis indicated that GAPDH of G. lemaneiformis might have undergone an evolution similar to those of Porphyra yezoensis, Chondrus crispus, and Gracilaria verrucosa. C. caldarium had a closer evolutionary relationship with Cyanidioschyzon merolae than with Cyanidium sp. Virtual Northern blot analysis revealed that gpd of G. lemaneiformis expressed constitutively, which suggested that it might be house-keeping and could be adapted as an inner control in gene expression analysis of G. lemaneiformis.  相似文献   

Molecular Cloning of a Cytosolic G6PDH Gene from Populus Suaveolens and Its Expression to Improve the Cold Resistance of Tobacco Plants     

LIN Yuan-zhen  GUO Hai  ZHANG Zhi-yi  LIN Shan-zhi 《冰川冻土》2009,31(6)

Glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) is the first and main regulated enzyme of oxidative pentose phosphate pathway (OPPP), catalyzing the conversion of glucose-6-phosphate to 6-phospho-gluconolactone and playing important roles in the growth and development of plants. It is preciously reported that the enhancement of freezing resistance of Populus suaveolens cuttings is clear related to the distinct increase in cytosolic G6PDH activity. Here, a 1697 bp cDNA fragment (PsG6PDH) is amplified by RT-PCR from cold-induced total RNA of the freezing-tolerant P. suaveolens . A sequence analysis showed that PsG6PDH coding region had 1 530 bp and encoded 510 predicted amino acid residues. Genomic Southern analysis revealed that the isoform is encoded by a few copies of the gene in the poplar genome. The cloned gene PsG6PDH is cloned into binary vector pBI121 and used to transform tobacco. PCR and Southern blotting results verified integration of this gene into the genome of tobacco. Moreover, cold treatment experiments and membrane defense enzyme activity analysis confirmed that overexpression of the PsG6PDH gene could enhance the tolerance to cold or frigid stresses in transgenic plants.  相似文献   

海洋脂肪酶ADM47601固定化方法的研究   总被引：1，自引：1，他引：0  

段晓琛  盛军  徐甲坤  郝建华  孙谧 《海洋与湖沼》2013,44(5):1311-1317

采用海藻酸钠、壳聚糖、聚乙烯醇和明胶等材料, 进行对脂肪酶ADM47601的固定化研究。结果表明, 使用壳聚糖固定化脂肪酶, 在最优条件为2% (W/V)壳聚糖, 10% NaOH, 1%乙酸, 0.25%戊二醛, 每克载体添加840U脂肪酶时, 最大固定化酶活力回收率为87.06%。使用海藻酸钠-明胶固定化脂肪, 在最优固定化条件下, 最大固定化酶活力回收率为54.45%。使用聚乙烯醇固定化脂肪酶, 在最优固定化条件下, 最大固定化酶活力回收率为33.22%。使用海藻酸钠固定化脂肪酶, 在最优固定化条件下, 最大固定化酶活力回收率为17.11%。对比四种不同固定化酶方法, 脂肪酶活力回收率高度高低顺序为: 壳聚糖吸附交联法>海藻酸钠明胶协同包埋法>聚乙烯醇-硼酸法>海藻酸钠包埋法。  相似文献   

Biodegradation Studies on Acid Violet 19, a Triphenylmethane Dye,by Pseudomonas aeruginosa BCH     

Shekhar B. Jadhav  Snehal M. Yedurkar  Swapnil S. Phugare  Jyoti P. Jadhav 《洁净——土壤、空气、水》2012,40(5):551-558

Acid violet 19 (AV) belongs to the triphenylmethane (TPM) class of dyes which are potentially mutagenic or carcinogenic. However, very little studies on biodegradation of AV were reported as compared to other TPM dyes such as malachite green and crystal violet. In this study, AV was decolorized up to 98% within 30 min by Pseudomonas aeruginosa BCH. The decolorization depends on the initial dye concentration, pH, and temperature. However, the dye was decolorized under wide pH and temperature ranges with an optimum of pH 7 and 30°C. Up to 250 mg L^?1 of dye was found to be tolerated and decolorized by this strain. It showed decolorization ability for seven repeated dye addition cycles. The effect of additional carbon sources on dye decolorization was studied in which mannitol containing medium showed decolorization in 15 min. Induction in the enzyme activities of laccase, NADH‐DCIP reductase, and veratryl alcohol oxidase (VAO) indicates their involvement in AV degradation. Various analytical studies viz. UV–VIS, HPTLC, HPLC, and FTIR confirmed the biodegradation of AV by the bacterium. Based on GC‐MS analysis, a possible degradation pathway for AV was proposed. The phytotoxicity studies using Phaseolus mungo and Sorghum vulgare revealed the less toxic nature of metabolites formed after AV degradation.  相似文献   

酒依赖致慢性酒精中毒性脑病的临床及CT/MRI分析     

余红胜  武宁强  沈又利 《CT理论与应用研究》2010,19(3):107-116

目的:分析酒依赖致慢性酒精中毒性脑病(CAE)的临床及CT/MRI影像表现特征,探讨CT/MRI在该病临床诊断中的价值。方法:回顾性分析32例资料完整CAE患者的临床及CT/MRI影像学资料,总结其影像学表现特征。结果:CAE临床表现主要为不同程度的神经系统损害(尤为中枢神经系统)和精神障碍,其颅脑损害CT/MRI主要表现为:脑萎缩27例;脑梗塞7例;白质脱髓鞘18例;韦尼克脑病3例;胼胝体变性1例;多病变并存24例。结论:①CAE的病理机制为饮酒导致的脑血管损害和营养代谢障碍,以及脑组织缺血缺氧、神经纤维脱髓鞘改变;②CAE影像学表现有其特征性,尤以小脑蚓部萎缩以及韦尼克脑病、胼胝体变性更具特征性,CT/MRI检查对了解脑损害的性质、范围及程度有重要意义,可为临床诊断、治疗及评估预后提供依据。  相似文献   

Ice- and cryogel-soil composites in water-retaining elements in embankment dams constructed in cold regions     

N. K. Vasiliev  A. A. Ivanov  I. N. Shatalin  V. V. Sokurov 《寒旱区科学》2013,5(4):0444-0450

There are a number of serious problems connected with building and repairing water-retaining elements in embankment dams in cold regions. One of them is the difficulty in finding inexpensive clay materials with the necessary structural properties and using them in the winter. Another is the cracks that appear in the upper part of a dam when the core freezes to the banks, and leakage along the cracks threatens to destroy the dam. Still another is the process of erosion at the bottom of the core which may occur through fissures in the rock foundation of a dam and in transport constructions. Also, the behaviour of water-retaining elements during earthquakes is unreliable. All of these problems can be solved by using ice- and cryogel-soil composites created by cryotropic gel formation (CGF). Our laboratory investigations demonstrate that the materials proposed for water-retaining elements have the necessary permeable, plastic, thermophysical, and strength properties to solve all of these problems. Certain constructions of water-retaining elements which may prove to be both safe and cost-effective are proposed. However, these ice- and cryogel-soil composites need to be field-validated before they are used in dams and transport structures in cold regions.  相似文献