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The biologically induced cementation (BioGrout) method has been used in this study to improve engineering properties of soil. Laboratory tests have been carried out to quantify the effect of BioGrout treatment on the strength of two types of sand by using Sporosarcina pasteurii bacteria. Bearing strength and other parameters, such as colony forming units (number of bacteria), pH level, temperature, and amount of CaCO3, have also been studied. Calcification (cementation) through precipitation of CaCO3 on the mineral surfaces has been clearly observed from the images of a scanning electron microscope (SEM). Finally, the effect of sand material on the effectiveness of biocementation was investigated via employement of different sand types.  相似文献   
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选用巴斯德芽孢杆菌,对微生物诱导钙离子、镁离子以及铁离子形成碳酸盐固化松散珊瑚砂颗粒的效果进行对比研究。试验结果表明,在相同试验方法、相同注入次数的条件下,微生物诱导形成的碳酸钙和碳酸镁能将珊瑚砂松散颗粒固化成为整体,加入铁离子的试样因为反应过程的原因没有成功。通过渗透性、干密度变化、无侧限抗压强度及微观结构等方面进行对比分析,钙离子试样相对于镁离子试样,具有固化过程平稳、干密度增量大、强度高、微观颗粒包裹更好的优点。综合试验结果得出,钙离子是现阶段微生物固化珊瑚砂较为理想的金属离子。本试验首次探讨了金属离子种类对珊瑚砂微生物固化效果的影响,为微生物固化技术提供了一定理论基础,对该技术的进一步应用具有一定借鉴意义。  相似文献   
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The mechanical efficiency of the biocementation process is directly related to the microstructural properties of the biocemented sand, such as the volume fraction of calcite, its distribution within the pore space, coordination number, contact surface area, and types of contact. In the present work, some of these microscopic properties are computed, from 3D images obtained by X-ray tomography of biocemented sand. These properties are then used as an input in current analytical models to estimate the elastic properties (Young and shear moduli) and the strength properties (Coulomb cohesion). For the elastic properties, the analytical estimates (contact cement theory model) are compared with classical bounds, self-consistent estimate and numerical results obtained by direct computation (FEM computation) on the same 3D images. Concerning the cohesion, an analytical model initially developed to estimate the cohesion due to suction in unsaturated soils is modified to evaluate the macroscopic cohesion of biocemented sands. Such analytical model is calibrated on experimental data obtained from triaxial tests performed on the same biocemented sand. In overall, the presented results point out the important role of some microstructural parameters, notably those related to the contact, on such effective parameters.  相似文献   
4.
Dredged or excavated soft marine clay can be improved by mixing it with cement or lime. However, these treatments are usually expensive. It is shown in this paper that soft marine clay can be strengthened through a bioencapsulation method in which the shear strength of clay aggregates can be substantially increased after the aggregates are treated with urease-producing bacteria, calcium chloride, and urea. We found that the bioencapsulation had increased the unconfined compressive strength of marine clay aggregates with a size of 5 mm from almost zero to more than 2 MPa. The strength of the bioencapsulated clay aggregates decreases with the increase in the size of the aggregate when the size is greater than 5 mm.  相似文献   
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