CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES     

郭华荣  张士璀  李红岩  童裳亮  相建海 《中国海洋湖沼学报》2002,20(3):226-231

Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro.  相似文献   

Geochemistry of porewater and sulfate-reducing bacteria in sediment cores from the Chianan Plain, Taiwan     

Lichun LIU Yuting CHEN Kuanyu CHEN Jyhyih LEU Tsungkwei LIU 《中国地球化学学报》2006,25(B08):67-67

High concentrations of arsenic and humic substances in groundwater from the southwestern coastal plain of Taiwan were well known for their probable relationships with black-foot disease. In order to realize the relationships between the concentrations of humic substances and arsenic in this area, 24 well water samples were analyzed. After filtered through 0.45 μm glass fiber membrane in the field, samples were kept in the dark at 4℃ and then separated into six fractions with varying range of molecular weight （〈0.5, 0.5-1, 1-5, 5-10, 10-50 and 〉50 k Da） by ultrafiltration apparatus （Molecular/Por Stirred Cell system） in the lab. Concentrations of humic substances were measured by fluorescence spectrometer （HITACHI F-2000, ex=370, em=445） and arsenic by FIAS-AA （Perkin Elmer AAnalyst 100, FIAS-400）. On average, only 6.2% of the total arsenic in water existed in the fraction of 〉0.5 k Da. and the others were complexed with humic substances （including humic acid and fulvic acid）. The results demonstrated a distinct positive correlation between the concentrations of humic substances and arsenic.  相似文献   

Assessment of marine pollution in Daya Bay, South China： Nutrients, heavy metals and persistent organic pollutants     

《中国地球化学学报》2006,25(B08):166-166

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Effect of particles on the photodegradation of PAHs in natural waters-A case study for the Yellow River     

Xinghui XIA 《中国地球化学学报》2006,25(B08):263-263

Photodegradation of chrysene, benzo （a） pyrene and benzo （g, h, i） perylene in natural water of the Yellow River was studied using simulation sunlight. The effects of particulates on the photodegradation were explored. Several results arose from this study. （1） The photodegradation of PAHs can be fitted with first-order kinetics when no particulate exists in water system, and the first-order constant increases with decreasing of initial concentration of PAHs. The photodegradation rates of the three PAHs are related to their molecule absorption spectrum. （2） The existence of loess exerts two kinds of effects on the photodegradation of PAHs, including the inner filter effects and the photosensitizing effects of humic substance in loess. These two contrary effects lead to the difference of net effects among different contents of loess. When the loess contents are 0.1 and 5.0 g/L, the existence of loess stimulates the photodegradation of chrysene, benzo （a） pyrene. When the loess content is 5.0 g/L, the existence of loess stimulates the photodegradation of benzo （g, h, i） perylene. In addition, the photodegradation of PAHs can be fitted with the second-order kinetics when there is loess in the water system. （3） The soluble humic substances in loess can accelerate the photodegradation of PAHs while the in soluble part cannot. （4） Since the soluble humic substances in the suspended solids of the river have been dissolved in water,  相似文献   

Dual simulations of fluid flow and seismic wave propagation in a fractured network: effects of pore pressure on seismic signature   总被引：1，自引：0，他引：1  

S. Vlastos  E. Liu  I. G. Main  M. Schoenberg  C. Narteau  X. Y. Li  B. Maillot 《Geophysical Journal International》2006,166(2):825-838

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大别山东部花岗岩类的稀土元素地球化学及其地质意义   总被引：1，自引：0，他引：1  

胡雄星  马东升 《矿产与地质》2002,16(6):321-326

在研究大别山东部主要花岗岩体的稀土元素地球化学特征、稀土元素配分型式的基础上 ,探讨了花岗岩体的物质来源、成岩模式及其构造意义。研究表明 :(1)大别山东部出露的主要花岗岩类岩体的物质来源基本相同 ,且源区具有古岛弧的特征 ;(2 )各岩体的成岩模式呈现以分离结晶作用为主的特征 ;(3)花岗质岩浆上侵的构造环境与中国东部燕山期构造转折的大背景有关。伸展拉张过程造成的地幔上涌不但是花岗岩类形成的构造条件 ,也对超高压变质岩的快速折返有重要影响。  相似文献   

MODELING THE FATE AND TRANSPORT OF HYDROPHOBIC ORGANIC COMPOUNDS IN AN UNSTEADY RIVER-ESTUARINE SYSTEM     

Su-Chin CHEN  Jan-Tai KUO 《国际泥沙研究》2002,17(3)

1 INTRODUCTION Extensive literature (Brown et al., 1985; Sawhney et al., 1981; Bierman and Swain, 1982; Connolly, 1980; Lopez-Avila and Hites, 1980; O扖onnor, 1988) described lots of sorbed pollutants or toxic substances in bed sediments of rivers, even after the effluent was halted for a long time. This is particularly true for hydrophobic organic compounds that can be sorbed on the particles and accumulated in the river bed sediments (Karickhoff et al., 1979). Pollution events of…  相似文献   

A cellular model of Holocene upland river basin and alluvial fan evolution   总被引：1，自引：0，他引：1  

T. J. Coulthard  M. G. Macklin  M. J. Kirkby 《地球表面变化过程与地形》2002,27(3):269-288

The CAESAR (Cellular Automaton Evolutionary Slope And River) model is used to simulate the Holocene development of a small upland catchment (4·2 km²) and the alluvial fan at its base. The model operates at a 3 m grid scale and simulates every flood over the last 9200 years, using a rainfall record reconstructed from peat bog wetness indices and land cover history derived from palynological sources. Model results show that the simulated catchment sediment discharge above the alluvial fan closely follows the climate signal, but with an increase in the amplitude of response after deforestation. The important effects of sediment storage and remobilization are shown, and findings suggest that soil creep rates may be an important control on long term (>1000 years) temperate catchment sediment yield. The simulated alluvial fan shows a complex and episodic behaviour, with frequent avulsions across the fan surface. However, there appears to be no clear link between fan response and climate or land use changes suggesting that Holocene alluvial fan dynamics may be the result of phases of sediment storage and remobilization, or instabilities and thresholds within the fan itself. Copyright © 2002 John Wiley & Sons, Ltd.  相似文献   

数字化射线照相技术对轻物质的判断     

韩裕生  罗娅  丁厚本  邵立康  廖南  朱平安 《CT理论与应用研究》2002,11(1):10-12

本文介绍了数字化射线照相技术对轻物质进行判断的方法。经实践证明，该方法是合理和有效的，可大大增强对轻物质的识别能力，是一种非常可行的识别技术。  相似文献   

基于神经网络的单元自动机CA及真实和忧化的城市模拟   总被引：6，自引：0，他引：6  

黎夏  叶嘉安 《地理学报》2002,(2)

提出了一种基于神经网络的单元自动机（ＣＡ）。ＣＡ已被越来越多地应用在城市及其它地理现象的模拟中。ＣＡ模拟所碰到的最大问题是如何确定模型的结构和参数。模拟真实的城市涉及到使用许多空间变量和参数。当模型较复杂时，很难确定模型的参数值。本模型的结构较简单，模型的参数能通过对神经网络的训练来自动获取。分析表明，所提出的方法能获得更高的模拟精度，并能大大缩短寻找参数所需要的时间。通过筛选训练数据，本模型还可以进行优化的城市模拟，为城市规划提供参考依据。  相似文献