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1.
参对光照变化非常敏感,研究刺参对光照的分子响应非常重要。本研究应用RNA测序获取了刺参暴露于强光(“强光”)、正常光照(“对照”)和完全黑暗(“黑暗”)环境下体壁的基因表达谱情况,通过“对照”与“黑暗”,“对照”与“强光”和“黑暗”与“强光”的比较,在|log2 ratio|≥1和FDR≤0.001的标准下分别发现了1161、113和1705个差异表达基因(DEGs)。基因本体分析表明,“cellular process”和“binding”在“生物过程”和“分子功能”类别中的DEGs富集最多,而“cell”和“cell part”在“细胞组分”类别中的DEGs富集最多。将DEGs与Kyoto Encyclopedia基因和基因组数据库上的于214、41和229条通路进行比对,发现了51、2和57条通路分别显著富集。本研究发现的光特异性DEGs可作为深入研究刺参对光照变化的生化适应机制的重要目标基因。 相似文献
2.
蛤蜊科3种贝类16SrRNA基因片段及ITS2核苷酸序列分析 总被引:4,自引:0,他引:4
利用PCR技术分别扩增连云港及启东沿海蛤蜊科的西施舌(Coelomactra antiquata)、中国蛤蜊(Mactra chinensis)和四角蛤蜊(Mactra veneriformis)3种双壳贝的16SrRNA基因片段和ITS2核苷酸序列.测序后用DNAstar软件分析了核苷酸差异。结果显示:三种贝类16SrRNA基因片段长度相同,均为306bp(去除引物).核苷酸存在多态性。共有45个变异位点,54个核苷酸发生了变异。全部为碱基置换。西施舌与中国蛤蜊此片段核苷酸的同源性为88.9%.与四角蛤蜊的同源性为88.6%.中国蛤蜊与四角蛤蜊的同源性为90.6%。三种蛤蜊ITS2序列分别为390bp(西施舌)、441bp(四角蛤蜊)和466bp(中国蛤蜊)。存在长度多态性.ITS2核苷酸差异分析结果显示.西施舌与中国蛤蜊的同源性为70.9%-71.1%,西施舌与四角蛤蜊的为70.5%-71.0%。中国蛤蜊与四角蛤蜊的同源性为88.1%-88.8%。ITS2序列分析结果与16SrRNA基因片段分析结果一致.2种分子分析法均显示中国蛤蜊与四角蛤蜊的亲缘关系近。 相似文献
3.
Larisa A. Yakovina Yakiv V. Pavlenko Carlos Abia 《Astrophysics and Space Science》2003,288(3):279-286
Lithium abundances in the atmospheres of the super Li-rich C-giants WZ Cas and WX Cyg are derived by the spectral synthesis
technique using the Li I resonance line at λ670.8 nm and three subordinate lines at λλ 812.6, 610.4 and 497.2 nm. The differences
between the Li abundances derived from the λ670.8 nm line and the λλ 497.2, 812.6 nm lines do not exceed ±0.5 dex. The lithium
line at λ610.4 nm provides typically lower abundances than the resonance line (by ≈ 1 dex). The mean LTE and NLTE Li abundances
from three Li I lines (excluding λ610.4 nm) are 4.7, 4.9 for WZ Cas, and 4.6, 4.8 for WX Cyg, respectively.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
4.
Nucleosynthetic yields and production rates of helium and heavy elements are derived using new initial mass functions which
take into account the recent revisions in O star counts and the stellar models of Maeder (1981a, b) which incorporate the
effects of massloss on evolution. The current production rates are significantly higher than the earlier results due to Chiosi
& Caimmi (1979) and Chiosi (1979), and a near-uniform birthrate operating over the history of the galactic disc explains the
currently observed abundances. However, the yields are incompatibly high, and to obtain agreement it is necessary to assume
that stars above a certain mass do not explode but proceed to total collapse. Further confirmation of this idea comes from
the consideration of the specific yields and production rates of oxygen, carbon and iron and the constraints imposed by the
observational enrichment history in the disc as discussed by Twarog & Wheeler (1982). Substantial amounts of4He and14C, amongst the primary synthesis species, are contributed by the intermediate mass stars in their wind phases. If substantial
numbers of them exploded as Type I SN, their contribution to the yields of12C and56Fe would be far in excess of the requirements of galactic nucleosynthesis. Either efficient massloss precludes such catastrophic
ends for these stars, or the current stellar models are sufficiently in error to leave room for substantial revisions in the
specific yields. The proposed upward revision of the12C (α,γ)16O rate may produce the necessary changes in stellar yields to provide a solution to this problem. Stars that produce most
of the metals in the Galaxy are the same ones that contribute most to the observed supernova rate. 相似文献
5.
6.
分析了普通番茄(Lycopersicumesculentum)在NaCl胁迫下酯酶(EST)和过氧化物酶(PRX)同工酶的变化,考察了盐胁迫对10个同工酶位点21个等位基因在特定组织中表达的影响。发现许多EST和PRX等位基因在盐胁迫下表达,产生盐诱导同工酶;另有些等位基因的表达受盐抑制,它们在盐胁迫下的表达活性显著减弱或消失。实验表明,EST和PRX同工酶表达的变化与番茄对盐胁迫的遗传适应性有密切关系。 相似文献
7.
采用PCR扩增和序列测定等技术,对远洋梭子蟹Portunus pelagicus核rDNA的第一内转录间隔区(internal transcribed spacer 1,ITS-1)的基因片段进行了初步研究。经PCR扩增和序列测定,得到ITS-1基因片段的碱基序列。该物种ITS-1基因片段的大小为552 bp,碱基A、T、G和C的含量分别为23.00%、25.54%、23.91%和27.54%。同时还对近年来ITS-1在十足目动物分子系统学研究中的应用作一概述并列举了已测出ITS-1序列的十足目动物种类。 相似文献
8.
日本(虫寻)和底栖短桨蟹线粒体DNA 12SrRNA基因序列的初步研究 总被引:6,自引:1,他引:6
以相应引物对日本和底栖短桨蟹的线粒体 DNA1 2 S r RNA基因片段进行了 PCR扩增和序列测定 ,分析比较了 2种间序列差异。结果表明 :日本 1 2 S r RNA基因片段长度为 41 4 bp,底栖短桨蟹为 41 5bp,2种的 A,T,G,C含量分别为 1 51 bp(36.47% ) ,1 53bp(36.96% ) ,43bp(1 0 .39% ) ,67bp(1 6.1 8% )和 1 50 bp(36.1 4 % ) ,1 55bp(37.35% ) ,44bp(1 0 .60 % ) ,66bp(1 5.90 % )。 2种间共出现了 3个碱基的缺失 /插入和 38bp的序列差异 ,其碱基转换与碱基颠换比约为 2 .1 7。 相似文献
9.
采用荧光显微技术,对2006年长江口及近海水域20个站点的表层及10m层或潜水体冬、春两季的浮游病毒丰度进行了检测,对浮游病毒丰度在季节(冬、春两季)、水平分布和垂直分布上的变化进行了探讨.调查区浮游病毒丰度在冬、春季节上并无明显差异,但在水平分布上存在很大差异,河口区浮游病毒直接检测量(Virus Direct Count, VDC)达到10^7个/ml,近海水域VDC为10^6个/ml,河口区的浮游病毒丰度都明显高于近海水域病毒丰度 (P<0.01).在垂直分布上,冬、春两季长江口水域水深小于10m的站位,表层浮游病毒丰度与底层病毒丰度无明显差别,水深大于10m的站位,表层水样的浮游病毒丰度都高于10m水层病毒丰度,说明长江口浮游病毒的垂直分布与站位总水深有关.还通过比较各站点VDC与叶绿素a含量的数据,分析了二者之间的相关性:冬季浮游病毒丰度与叶绿素a含量成正相关性;春季浮游病毒丰度与叶绿素a含量成负相关性,但病毒丰度受叶绿素a含量的影响仅为10%-11%. 相似文献
10.
Lina Li Jean Guenzennec Peter Nichols Pierre Henry Miki Yanagibayashi Chiaki Kato 《Journal of Oceanography》1999,55(5):635-642
Dense populations of bivalves, primarily Calyptogena sp., were observed at cold seeps of the Nankai Trough. Bacterial input to the sediment was estimated through determination of phospholipid ester-linked fatty acid (PLFA) and DNA profiles. Results indicated a bacterial biomass of 109 cells (g dry wt)-1 while individual fatty acid profiles revealed a predominance of monounsaturated fatty acids, mainly 18:1 isomers. The presence of these fatty acids can be interpreted to reflect a response to low temperature and a predominance of psychrophilic bacteria. DNA fragments encoding bacterial ribosomal RNA small-subunit sequences (16S rDNA) were amplified by the polymerase chain reaction method using DNA extracted directly from the sediment samples. From the sequencing results, at least 19 kinds of bacterial 16S rDNAs related to mostly the Proteobacteria and a few gram-positive bacteria were identified. These results suggest that the bacterial community in the Nankai Trough sediments consists of mainly bacteria belonging to the Proteobacteria , , and subdivisions. Bacteria belonging to the and subdivisions, which are known to include epibiont and sulfate reducing bacteria, respectively, were mostly detected in the sediment obtained from inside the area of the Calyptogena community, and the -Proteobacteria may function to supply reduced sulfur to bacterial endosymbionts of Calyptogena. 相似文献