耳鲍(Haliotis asinina)核糖体小亚基(18S rRNA)编码基因的克隆与序列分析   总被引：1，自引：0，他引：1       下载免费PDF全文

黄勃  方再光  刘均玲  周智  王小兵 《海洋与湖沼》2007,38(3):212-216

采用分子生物学的方法,对南海不同海区的两个地理群体耳鲍(Haliotis asinina)18S rRNA基因全长进行了克隆和序列分析,并将耳鲍18S rRNA基因的序列与NCBI数据库中已收录鲍的18SrRNA基因进行了比较。结果发现,南海耳鲍核糖体18S rRNA基因与耳鲍H.asinina isolate H11核糖体18S rRNA基因序列的相同率高达98%;同一地理群体内耳鲍核糖体18S rRNA基因序列完全一致;不同地理群体间耳鲍核糖体18S rRNA基因在碱基组成上的相似率为99%,仅在某些位点处发生了碱基替换,即腺嘌呤(T)被鸟嘌呤(G)替换;同时,将这两个不同群体中耳鲍的18S rRNA基因与泰国耳鲍18S rRNA基因序列进行比较分析发现,它们之间也只是发生了碱基替换。  相似文献   

棉粕蛋白酶解物对异育银鲫(Carassius auratus gibelio)消化、生长和胰蛋白酶mRNA表达量的影响     

刘文斌  王恬 《海洋与湖沼》2006,37(6):568-574

以棉粕蛋白为酶解底物,用枯草杆菌蛋白酶对其进行酶解,以酶解产物1.5%和3.0%两个梯度等量替代鱼饲料配方中棉粕,在室内流水养殖系统中喂养异育银鲫鱼种[体重为(30±2)g]65天。测定鱼的生长、营养物质表观消化率、消化蛋白酶活性及肝胰脏中胰蛋白酶mRNA表达水平等指标。结果表明,添加1.5%和3.0%棉粕酶解产物的鱼在饲养35天后的特定增长率(SGR)分别比对照组高32.5%和56.7%,且差异显著(P<0.05);在饲养65天后,两组特定增长率分别比对照组高8.0%和21.0%,且差异极显著(P<0.01),肝胰脏中胰蛋白酶mRNA表达水平也随棉粕酶解产物添加梯度提高而相应提高,表明鱼的生长与消化蛋白酶mRNA表达水平相关。同时棉粕蛋白酶解物对肠道蛋白酶的活性和营养物质表观消化率都有促进作用,而棉粕蛋白酶解物对鱼肌肉成分并没有改变,这也表明棉粕蛋白酶解物在促进鱼生长、内源酶活性同时并未降低鱼的品质。  相似文献   

刀额新对虾染色体核型及细胞核DNA含量   总被引：9，自引：0，他引：9  

张晓军  周岭华  相建海 《海洋与湖沼》2002,33(3):225-231

以刀额新对虾胚胎、无节幼体、卵巢、精巢等为材料 ,空气干燥法制备染色体 ,并初步进行核型分析。结果表明 ,刀额新对虾的染色体数目为 2n=78,n =39,核型为 2n=78=40M 1 0SM 1 4ST 1 4T。以刀额新对虾血淋巴、卵巢、肌肉、鳃为材料 ,以鸡血细胞为DNA标准 (2 50pg/ 2c) ,使用PartecCCAⅡ型流式细胞仪测定了刀额新对虾细胞的基因组DNA含量 ,其值为鸡血细胞的 1 75倍 ,绝对含量为 4 375pg/ 2c。  相似文献   

基于DSP的水下图像传输系统研究     

程恩  许茹  苏运东 《海洋技术学报》2001,20(3):44-48

本文研究了基于高性能的DSP芯片TMS320C549的水下图像传输系统，并采用Goertzel算法进行信源编码和MFSK调制方式、运用Turbo码进行信道的编、解码，来实现水下视频图像高速数据传输的目的。  相似文献   

Summer Arctic sea fog     

Xie Simei  Xue Zhenhe  Jiang Dezhong  Zou Bin  Qu Shaohou 《海洋学报(英文版)》2001,20(2):183-196

I~IOXSea fog is a kind of dangerous weather. Chinese sea fog experts, Wang Binhua (1983),Hu Ruijin and Zhou Faxiu (1998) and Hu Jifu et al. (1996) studied sea fog rather Systematically. FOreign Experts also Paid great attention to sea fog. Ernlnons and Montgomery(1974), chipper (1994) and Rayrnond et al. (1989) have studied sea fog thorOUghly.HOwever, studies on Arctic sea ice have rarely been carried Out becauSe of the sever environment and less htnnan activity in the region. There …  相似文献   

日本鳗鲡早期阶段耳石日生长轮形成的周期   总被引：7，自引：0，他引：7  

李城华  沙学绅 《海洋与湖沼》1995,26(4):408-413

于1990年4月从江苏太湖搜集日本鳗鲡亲鱼暂养在天津滨海虾场8t玻璃钢水槽中，经人工催熟催产后孵出仔鳗，对孵出仔鳗连续取样；于1989年4月在长江口采集白仔鳗，观察和比较二者的耳石生长轮形成。结果表明，（1）人工繁殖仔鳗耳石第一个生长轮是在孵出后第一天形成的，轮纹形成具有24h周期性；仔鳗孵出后生长天数与生长轮数关系的回归方程以y=0．23＋0．91x表示，其中，x为孵出后的天数，y为生长轮数;在人工繁殖仔鳗耳石上没有观察到“孵化标记”轮。（2）白仔鳗耳石中心核与“孵化标记”轮之间存在日生长轮。（3）人工繁殖仔鳗与白仔鳗耳石中心核同第一个生长轮比较表明，前者小于后者。  相似文献   

Three-dimensional numerical experiments on tidal exchange through a narrow strait in a homogeneous and a stratified sea     

Norihisa Imasato  Shinzou Fujio  Qian Zhang  Toshiyuki Awaji  Kazunori Akitomo 《Journal of Oceanography》1994,50(2):119-139

We have investigated the three-dimensional Lagrangian motion of water particles related with tidal exchange between two basins with a constant depth connected through a narrow strait and the effects of density stratification on the exchange processes by tracking a number of the labeled particles. Tide-induced transient eddies (TITEs), which are similar to those in two-dimensional basin, are generated behind the headlands. Upwelling appears around the center of the eddy and sinking around the boundary. When the basins are filled with homogeneous water, a pair of vortices are produced in the vertical cross section of the strait due to bottom stress, with upwellings along the side walls of the strait and sinking in the center of the strait. These circulations form the horizontally convergent field in the cross-strait direction in the upper layers while the horizontal divergence takes place in the bottom layer. These vertical water-motions produce the three-dimensional distribution of velocity shear and phase lag of the tidal current around the strait, and the Lagrangian drifts of water particles become large. As a result, water exchange through the strait is greatly enhanced: The water exchange rate reaches 94.1% which is much larger than that obtained in the vertically integrated two-dimensional model. When the basins are stratified, the stable stratification suppresses the vertical motion so that a pair of vertical vortices are confined in the lower layers. This leads to a decrease in the exchange rate, down to 88.6%. Our numerical results show that the three-dimensional structure of tidal currents should be taken into account in tidal exchange through a narrow strait.  相似文献   

A new type of Shipboard Meteorological Satellite Receiving-processing System   总被引：1，自引：0，他引：1  

Xie Simei  Hao Chunjiang  Zou Bin  Jing Qiyi  Liao Xiaohong  Sheng Xiaowen  Zhang Canrong 《海洋学报(英文版)》2000,19(1):35-46

INTRODUCTIONTheSMSRPSisstalledontheshipnavigatingovertheseaandreceivessatellitecloudmapsatanytimetoprovidereliablereal-timedataofmeteorologyandocean.Itisanimportantequipmentforsafeguardofshipnavigation.Chinahasthousandsofoceanicships.Butalmostallships,withonlyveryfewexception,arenotstalledbySMSRPS.Thecausesareasfollows:1.Thetechniqueisverycomplicated.2.Thedevelopmentcostisveryhigh.3.Ifweintroduceforeignequipment,thecostistooexpensive.4.Foreignequipmentanditssoftwarearecompletelyclo…  相似文献   

南大洋深海嗜冷菌2-5-10-1及其低温脂肪酶的研究   总被引：14，自引：0，他引：14  

林学政  杨秀霞  边际  黄晓航 《海洋学报》2005,27(3):154-158

从南大洋普里兹湾的水样中筛选到一株产低温脂肪酶的深海嗜冷菌2-5-10-1,对其生长及产酶情况和酶性质做了初步研究.该菌株的最适生长温度为5℃,此时分泌的胞外脂肪酶最多;添加Tween80,橄榄油可显著促进脂肪酶的产生.该脂肪酶的最适作用温度为35℃,在0～20℃均保持较高的酶活性,在0℃可保持37%的相对酶活性;酶的最适作用的pH值为7.5,在pH6～9的范围内均存在较高酶活性;对热较敏感,在60℃保温15min可丧失50%以上的酶活性.该脂肪酶的催化作用不需要金属离子的参与,Cu2+和Zn2+对酶活有着强烈的抑制作用.  相似文献   

Rapid detection of virulent protease secreted by Vibrio anguillarum by dot enzyme-linked immunosorbent assay     

ZHANG Zhendong  ZHANG Peijun  MO Zhaolan  WANG Chunling  YU Yang 《海洋学报(英文版)》2005,24(4):155-161

Dot enzyme-linked immunosorbent assay （dot-ELISA）, indirect ELISA and Westem blot were performed to detect the virulent protease secreted by Vibrio anguillarum which was isolated from the diseased left-eyed flounder, Paralichthys olivaceous. Sensitivity results showed that dot-ELISA is a more sensitive, rapid and simple technique for the protease detection. The minimal detectable amount of protease is about 7 pg in the dot-ELISA test, while 7.8 ng in the indirect ELISA and 6.25 ng in the Westem blot respectively. Protease could be detected 2 h after incubation of V. anguillarum in the 2216E liquid medium but enzyme activity was very low at that period. From 6 to 12 h, the amount and enzyme activity of protease increased markedly and reached maximum at stationary phase. Analysis of serum samples periodically collected from the infected flounders showed that after 2 h of infection by V. anguillarum, the pathogenic bacteria could be detected in the blood of the infected flounders but no protease was found. It was 5-6 h after infection that the protease was detected in blood and then the amount increased as infection advanced. Quantitative detection of protease either incubation in the medium or from the blood of infected flounders could be accomplished in virtue of positive controls of quantificational protease standards （＂marker＂） so that the alterations ofprotease secretion both in vitro and in vivo could be understood generally. In addition, the indirect ELISA and dot-ELISA were also performed to detect V. anguillarum cells. Results indicated that the sensitivity of indirect ELISA to bacteria cells is higher than that of the dot-ELISA, and that the minimal detectable amount is approximately 10^4 cell/mL in the indirect ELISA, while 10^5 cell/mL in the dot-ELISA.  相似文献