长牡蛎碱性磷酸酶的分离纯化及部分性质研究   总被引：5，自引：1，他引：5  

李兴暖  韩雅莉  肖湘  邓瑞鹏  周小朋  管云雁  黄长江 《台湾海峡》2003,22(4):482-486

本文以长牡蛎为酶源提取材料,用正丁醇抽提,硫酸铵分级分离,DEAE-52和Sephadex G-100柱层析,得到一定纯度的碱性磷酸酶,提纯倍数为61.96,比活力达1.326U/mg.该酶的最适pH值9.6,最适温度38℃,初速度6.8μmol/(dm~3·min),米氏常数Km值为1.30mmol/dm~3.实验结果表明,Mg~(2+)对该酶有明显的激活作用;Zn~(2+)、Cu~(2+)、Hg~(2+)对该酶有明显的抑制作用.  相似文献   

合浦珠母贝26S蛋白酶体S4,S7亚基基因片段的分离与分析     

姜铁民  张勇  黄洁  谢莉萍  吴移谋  张荣庆 《海洋科学》2003,27(4):28-34

26S蛋白酶体是真核生物中一种具有ATP依赖性的蛋白酶复合体,主要通过泛肽途径选择性降解细胞内与代谢调控、细胞周期有关的功能蛋白及异常蛋白,参与多种细胞活动的调控过程。26S蛋白酶体由具有催化活性的20S亚复合体和一个具有调节作用的19S亚复合体组成,其中19S亚复合体中的ATP酶亚基是调节26S蛋白酶体活性的重要组件。本通过简并引物PCR手段,从软体动物合浦珠母贝(Pinctada fucata)中扩增到参与构成19S亚复合体的S4和S7(MSS1)两个亚基的基因片段。这两个基因片段所编码的ATP酶组件包含有Gx4GKT,DEID,SAT和H／QRxGRxxR等26S蛋白酶体ATP酶亚基的共同功能基序。这是首次在软体动物中报道26S蛋白酶体的ATP酶亚基基因序列,为研究软体动物中26S蛋白酶体的结构与功能奠定了分子基础。  相似文献   

Numerical Simulation of Dynamic Response of Supple Nets     

朱立新梁振林  黄六一赵芬芳 《中国海洋工程》2006,20(3):443-456

1 .Introduction Owing to the decline of capture fisheries andsaturation of traditional inshorefarms ,sea cage cul-ture as a newtype of aquaculture is developingrapidlyin China .However ,cage culture is a highriskoperation.The practice is vulnerable to natural hazards ,such as strong tides ,storms and typhoons .So,it is necessaryto assess the behavior of a sea cage exposedto waves and current before the designandinstallation of the structure . Computer simulation,due to its many advantages ,ha…  相似文献   

基于GIS的柘林湾生态信息系统     

黎裕成  陈志远  黄长江  谢文勇  杜虹 《台湾海峡》2005,24(2):216-222

柘林湾生态信息系统以ArcGIS为开发平台，采用客户端／服务器的体系结构，基于ArcObjects的组件式二次开发而成，实现了柘林湾的三维实景模拟、生态数学模型与GIS的有效集成、分析评价结果与地理空间数据相结合等功能，具有可视化、智能化、易扩展、安全实用等特点．该系统的建成．极大地提高了柘林湾生态信息的现代化管理水平，并为制定柘林湾的污染防治与生态环境保护策略提供了强有力的支持．  相似文献   

Action of Cnoidal Waves on Vertical Walls     

Qiu Dahong  Zang Jun  Jia Ying Academician of the Chinese Academy of Sciences  Professor  the State Key Laboratory of Coastal  Offshore Engineering 《中国海洋工程》1996,(2)

Based on the 2nd order cnoidal wave theory, the characters of shallow water standing waves and their action on vertical walls are studied in this paper. The theoretical expressions of the wave surface elevation in front of and the wave pressure on the vertical wall are obtained. In order to verify the theoretical results, model tests were made in the State Key Laboratory of Coastal and Offshore Engineering at DUT. For the wave surface elevation in front of the wall and the wave forces on the wall at the moment when the wave surface at the wall surface goes down to the bottom of the wave trough, the calculated results coincide quite well with the experimental results. For the wave forces on the wall at the moment when the wave surface at the wall surface goes up to the top of the wave crest, the theoretical expressions are modified by the experimental results. For the convenience of practical use, calculations are made for the wave conditions which usually occur in enginering practice by use of the inves  相似文献   

南海季风型海-气耦合模态的时空特征及其与中国夏季降水的关系   总被引：2，自引：0，他引：2  

黄菲  王宏  戴平 《中国海洋大学学报(自然科学版)》2007,37(3):351-356

使用NCEP/NCAR的海表温度(SST)、海面10 m风场的月平均再分析资料,用联合SVD(CSVD)的方法研究了不同季节南海的海气耦合模的时空分布特征及其与中国夏季降水的关系。通过对不同季节的海-气耦合模的年际变化特征的分析。结果发现:第一模态为最显著模态,模态协方差贡献比在四季均超过80%,空间上SST表现为与南海等深线相一致的海盆模态,风场上主要表现为弱的冬季风或弱的夏季风,各个季节的海-气耦合模态都主要反映了SST-蒸发-风反馈这样1种正反馈的海-气相互作用过程,而且冬季风期间这种相互作用要更强烈些。时间系数均主要表现为一致的上升趋势和1976年前后的年代际突变,以及与ENSO相关的年际变化特征。冬、夏季弱的季风对应暖SST的特征体现了这种耦合模态隔季相关的特征,都对应夏季华南旱(涝)、江南涝(旱),华北、山东半岛旱(涝),东北涝(旱)这样1种波列状的旱涝相间分布。  相似文献   

Test Study on Current Force on Mooring Ships     

Zhang Furan  Zhao Jun Zhang Xueqin Assistant Researcher  Tianjin Research Institute of Water Transport Engineering  Tianjin Assistant Engineer  Tianjin Research Institute of Water Transport Engineering  Tianjin 《中国海洋工程》1993,(2)

In respect of an offshore berthing pillar, the test study result of current force acting on mooring ships is described in this paper. Empirical and semi-empirical relationships of current force coefficient are given according to the angle between the flow direction and the ship's longitudinal axis, which are coincident with the result of theoretical analysis.  相似文献   

A Computational Model for Velocity Separation in Shallow Sea   总被引：1，自引：0，他引：1  

SONG  Zhiyao 《中国海洋工程》2002,16(3):407-413

Based on the hydrodynamical feature and the theoretical velocity profiles of tidal flow and vvind-induced flow in shal-low sea, a computational model is established for the first time, which can separate observed velocity into tidal velocity and wind-induced velocity by use of the least square method. With the model, not only the surface velocities of tidal flow and vvind-induced flow are obtained, but also the bed roughness height is determined and the wind velocity above the wa-ter surface is estimated. For verification of the model, the observed velocity in the Yellow River Estuary and the laborato-ry test is separated, then it is applied to the Yangtze River Estuary. All the results are satisfactory. The research results show that the model is simple in method, feasible in process and reasonable in result. The model is a valid approach to analysis and computation of field dala, and can be applied to separate the observed velocity in shallow sea; at the same time, reasonable boundary conditions of th  相似文献   

冲绳海槽中段沉积岩芯碳酸盐和烧失量的古环境意义     

吴世迎  刘焱光  王湘芹  白黎明  吴军瑞 《海洋科学进展》2001,19(2):17-24

对冲绳海槽中段 3个沉积岩芯碳酸盐和烧失量的系统分析得知 ,岩芯沉积物的CaCO3含量一般 >1 0 % ,最高含量接近 30 % ,烧失量大都在 1 0 %～ 30 %之间变化 ,反映了该海区处于溶跃面之上钙质生物极为发育的生物地球化学环境。结合δ1 8O曲线所反映的气候环境变化 ,探讨了 1 2 0kaB .P .以来碳酸盐旋回的“大西洋型”演变特点 ,同时对该区沉积地层中的火山热液活动印记作了一定的探讨。  相似文献   

PROBABILITY CHARACTERISTICS OF WAVE AND WAVE PRESSURES IN FRONT OF VERTICAL BREAKWATER     

黄培基  赵炳来 《海洋学报(英文版)》1985,4(3):490-501

The distribution of wave height in front of a vertical breakwater, the range of wave pressure fluctuation at different subsurface levels, and the wave periods have shown that they are practically invariable with depth and can be determined theoretically. The distribution of crest and trough of total pressures is the same as that of wave height. As far as different subsurface levels are concerned, the spectral constitution of wave pressure field and the regularity of attenuation of spectral components are analyzed at the vertical breakwater, and a new expression describing the equilibrium range of wave pressure spectrum obtained.  相似文献