中国卤虫早期胚胎发育相关基因的研究--mRNA差异显示技术的三种不同染色方法比较   总被引：1，自引：0，他引：1  

林盛国  侯林  陈志娟  徐明玉  王璐 《海洋湖沼通报》2005,(1):67-72

利用mRNA差异显示技术研究中国孤雌生殖卤虫胚胎发育的相关基因，并对中国孤雌生殖卤虫差异显示中SYBR Green Ⅰ、硝酸银和溴化乙锭三种染色方法进行了比较。以三种单碱基锚定引物对发育0h，4h，8h，12h，16h和10天的中国孤雌生殖卤虫的RNA进行逆转录和26种随机引物进行PCR反应，经非变性的6％聚丙稀酰胺凝胶电泳后，分别用SYBR Green Ⅰ、硝酸银和溴化乙锭染色，最后进行差异条带的回收和再扩增。结果表明：(1)6种样本的DD-PCR产物显示长200-3．0k不等的扩增片段，条带清晰，并呈现出有无和强弱的变化(2)SYBR Green Ⅰ和硝酸银染色的灵敏度比溴化乙锭染色的灵敏度高(3)经SYBR Green Ⅰ和溴化乙锭染色的凝胶的回收和再扩增效率比硝酸银染色的高(4)水煮法和NAP-25过柱法对凝胶片段的回收效果几乎相当。利用mRNA差异显示技术研究中国孤雌生殖卤虫胚胎发育相关基因时，得到了较好的扩增片段；三种染色方法中SYBR Green Ⅰ染色灵敏度较高，并且SYBR Green Ⅰ染色有利于凝胶片段的回收和再扩增。  相似文献   

厦门港岩相潮间带多毛类的生态   总被引：2，自引：0，他引：2  

郑凤武  吴启泉 《台湾海峡》1997,16(1):85-89

本文根据１９８７年２、５、８、１１月调查资料，研究了厦门港岩相潮间带多毛类的种类组成、种类与数量的垂直分布、季节变化，并与大亚湾多毛类生态进行比较。结果表明，调查区域有多毛类４７种。平均栖息密度６０个／ｍ＾２，平均生物量１．７８ｇ／ｍ＾２，潮汐作用影响多毛类的种数、密度、生物量的垂直分布。多毛类数量的季节变化不甚明显，但冬季的栖息密度和春季的生物量较高。  相似文献   

黄海北部降水中氟的浓度变化   总被引：1，自引：0，他引：1       下载免费PDF全文

董峻  王剑  吕翔  张经 《海洋科学》1993,17(3):43-47

1988～1992年连续采集了黄海西北部的大气降水,分析样品中的氟而得到的80多个数据显示出大气降水中氟的平均浓度低于中国北方主要河流的氟平均浓度。在冬季,氟的浓度较其他季节高,这可能和降雨量减少以及人为因素等密切相关。目前还难以估算出人为因素对氟在降水中化学行为的影响程度,但数据显示出人口密集地区降水中氟的浓度明显高于人口稀疏的自然地带。  相似文献   

锌对虾夷扇贝和刺参幼体的毒性效应   总被引：11，自引：1，他引：11  

朱丽岩  吕建发  唐学玺  高象贤  徐怀恕 《海洋通报》1999,18(4):34-37

以虾夷扇贝和刺参幼体为材料,采用急性毒性试验和亚急性毒性试验的方法,研究了锌对虾夷扇贝稚贝及幼贝和刺参幼虫的毒性效应。结果显示,锌对虾夷扇贝稚贝的２４ｈＬＣ５０ ６．４０ｍｇ／Ｌ,４８ｈＬＣ５０为３．３１ｍｇ／Ｌ,７２ｈＬＣ５０为１．３９ｍｇ／Ｌ,９６ｈＬＣ５０为０．６１ｍｇ／Ｌ;而对幼贝的２４ｈＬＣ５０为３．３２ｍｇ／Ｌ,４８ｈＬＣ５０为２．６４ｍｇ／Ｌ。７２ｈＬＣ５０为１．７６ｍｇ／Ｌ,９６ｈ  相似文献   

海底油气管道保护电位检测装置的研究     

郭津年  徐斌  卢阿平  曾仁森  吴佳林 《海洋科学》1990,14(5):53-57

  相似文献   

地图误差对地图匹配质量的影响     

LIU Zhi-de  CHEN Jia-bin  DING Lu  SONG Chun-lei 《测绘学院学报》2008,(5)

高精度的数字地图是正确匹配车辆位置的基础。详细分析了地图数据的地理误差和拓扑误差的误差形式,路网数据模型的常见误差因素和改进策略,最后介绍了不同地图匹配算法对地图质量的敏感性和可行性。根据可能出现的误差对现有数字地图和匹配算法加以改进,弥补了原有数字地图带来的不精确缺陷。跑车实验证明,考虑了数字地图误差影响的匹配算法可以明显提高定位精度,减小车辆定位误差。  相似文献   

晚新生代亚洲干旱气候发展与全球变冷联系的风尘沉积证据   总被引：14，自引：8，他引：6       下载免费PDF全文

鹿化煜  王先彦  李郎平 《第四纪研究》2008,28(5):949-956

在干旱气候背景下常常有风尘物质的释放和传输,风尘沉积被认为是干旱环境变化良好的地质记录。在亚洲中部和中国北方,大面积的干旱区被沙漠戈壁覆盖,这些地区是粉尘物质的重要源地,释放的风尘物质影响局地和区域环境,并通过参与生物地球化学循环等影响更大空间尺度的气候变化。因此,亚洲中部干旱气候的形成和演化以及发展趋势一直受到重视。长期以来,关于晚新生代亚洲中部干旱气候发展的驱动机制至少有两种解释:一是青藏高原的隆升控制着亚洲中部的干旱化过程,随着高原的阶段性隆升亚洲干旱气候逐步增强;二是晚新生代全球变冷直接驱动着亚洲变干,全球变冷是主导因素。中国黄土高原及其周边地区堆积的黄土-红粘土序列是指示亚洲干旱化过程良好的地质记录,通过对新获得的风尘堆积记录的分析,发现在晚新生代中国风尘堆积的时空演化与全球变冷有较好的对应,全球变冷可以促使亚洲中部干旱气候发展并加强粉尘活动,而青藏高原隆升的幅度和时间还不清楚,进而认为是全球变冷而不是青藏高原隆升直接驱动亚洲内陆的阶段性变干。基于这些结果,我们认为以前关于青藏高原隆升影响全球气候的结论可能高估了青藏高原在地球环境演化中的作用,关于高原隆升直接驱动亚洲干旱化的结论还需要检验。  相似文献   

从地球系统科学角度浅析中国地貌若干问题研究的新进展   总被引：10，自引：3，他引：7       下载免费PDF全文

杨小平  师长兴  李炳元  朱秉启 《第四纪研究》2008,28(4):521-534

以地球系统中各圈层的相互作用为主线,分析总结了我国沙漠地貌过程、流水地貌过程、冰川地貌过程及风沙地貌过程等领域研究的部分进展。由于我国沙漠地理位置的特殊性,其形成和演变与岩石圈构造变动即青藏高原隆升有着紧密的联系,所以,对我国沙漠形成、演变的研究能为探讨青藏高原隆升历史提供重要佐证。近30年来,有关我国沙漠形成时代的认识更新较快。新的沉积记录显示,我国西北地区的沙漠在中新世时就已经出现了,但沙漠沙丘大规模扩展可能是在中更新世才开始的。既使在晚更新世以来,我国沙漠地区的气候也有过明显波动、沙漠地貌的特征也发生过显著变化。沙漠通过为沙尘暴提供物源,对全球变化产生驱动作用。从地表过程来看,风沙地貌的形成演变不仅受风力作用,而且受流水、湖泊等多种地貌动力过程的影响,地貌类型是各种动力过程共同作用的缩影。古冰川地貌曾是最早发现的第四纪气候变化的证据。随着新的测年技术的出现,学术界对我国第四纪古冰川地貌演化过程有了较系统、全面的认识。流水地貌过程应该是地球上作用区域最广的一种地貌动力过程,对流水地貌过程的认识目前正在向微观和宏观深入。  相似文献   

A sub-pixel analysis of urbanization effect on land surface temperature and its interplay with impervious surface and vegetation coverage in Indianapolis,United States     

Qihao Weng  Dengsheng Lu 《International Journal of Applied Earth Observation and Geoinformation》2008

This study developed an analytical procedure based upon a spectral unmixing model for characterizing and quantifying urban landscape changes in Indianapolis, Indiana, the United States, and for examining the environmental impact of such changes on land surface temperatures (LST). Three dates of Landsat TM/ETM+ images, acquired in 1991, 1995, and 2000, respectively, were utilized to document the historical morphological changes in impervious surface and vegetation coverage and to analyze the relationship between these changes and those occurred in LST. Three fraction endmembers, i.e., impervious surface, green vegetation, and shade, were derived with an unconstrained least-squares solution. A hybrid classification procedure, which combined maximum-likelihood and decision-tree algorithms, was developed to classify the fraction images into land use and land cover classes. Correlation analyses were conducted to investigate the changing relationships of LST with impervious surface and vegetation coverage. Results indicate that multi-temporal fraction images were effective for quantifying the dynamics of urban morphology and for deriving a reliable measurement of environmental variables such as vegetation abundance and impervious surface coverage. Urbanization created an evolved inverse relationship between impervious and vegetation coverage, and brought about new LST patterns because of LST's correlations with both impervious and vegetation coverage. Further researches should be directed to refine spectral mixture modeling by stratification, and by the use of multiple endmembers and hyperspectral imagery.  相似文献   

Application of Spectral Decomposition to Detection of Fracture-Cavity Carbonate Reservoir Beds in the Tahe Oiifieid, Tarim Basin, NW China     

Liu Xiaoping  Yang Xiaolan  Zhang Yazhong  Han Long 《《地质学报》英文版》2008,82(3):530-536

Ordovician fracture-cavity carbonate reservoir beds are the major type of producing formations in the Tahe oilfield, Tarim Basin. The seismic responses of these beds clearly changes depending on the different distance of the fracture-cavity reservoir bed from the top of the section. The seismic reflection becomes weak or is absent when the fracture-cavity reservoir beds are less than 20 ms below the top Ordovician. The effect on top Ordovician reflection became weaker with deeper burial of fracture-cavity reservoir beds but the developed deep fracture-cavity reservoir beds caused stronger reflection in the interior of the Ordovician. This interior reflection can be divided into strong long-axis, irregular and bead string reflections, and was present 80 ms below the top Ordovician. Aimed at understanding reflection characteristics, the spectral decomposition technique, which uses frequency to ＂tune-in＂ bed thickness, was used to predict Ordovician fracture-cavity carbonate formations in the Tahe oilfield. Through finely adjusting the processing parameters of spectral decomposition, it was found that the slice at 30 Hz of the tuned data cube can best represent reservoir bed development. Two large N-S-trending strong reflection belts in the mid-western part of the study area along wells TK440- TK427-TK417B and in the eastern part along wells TK404-TK409 were observed distinctly on the 30 Hz slice and 4-D time-frequency data cube carving. A small N-S trending reflection belt in the southern part along wells T403-TK446B was also clearly identified. The predicted reservoir bed development area coincides with the fracture-cavities connection area confirmed by drilling pressure testing results. Deep karst cavities occur basically in three reservoir bed-development belts identified by the Ordovician interior strong reflection. Spectral decomposition proved to be a useful technique in identifying fracture-cavity reservoir beds.  相似文献