Simple determination of dimethylsulfide (DMS) and dimethylsulfoniopropionate (DMSP) using solid-phase microextraction and gas chromatography-mass spectrometry     

Takushi?NikiEmail author  Taiki?Fujinaga  Mariyo?F.?Watanabe  Junji?Kinoshita 《Journal of Oceanography》2004,60(5):913-917

Solid-phase microextraction (SPME) is a simple, sensitive and less destructive method for the determination of dimethylsulfide (DMS) in seawater. Combined with detection by gas chromatography-mass spectrometry (GC-MS), the method had sufficient sensitivity (minimum detectable concentration of DMS was 0.05 nM), and practical levels of reproducibility (relative standard deviation ≤7%) and linearity (r ² > 0.995) over a wide concentration range (0.5 to 910 nM). The protocol developed was applied to a Sagami Bay water sample to determine concentrations of DMS and DMSP, and in situ DMSP-lyase activity.  相似文献   

Eddy Shedding from the Kuroshio Bend at Luzon Strait   总被引：16，自引：1，他引：16  

Yinglai?JiaEmail author  Qinyu?Liu 《Journal of Oceanography》2004,60(6):1063-1069

TOPEX/POSEDIENT-ERS satellite altimeter data along with the mean state from the Parallel Ocean Climate Model result have been used to investigate the variation of Kuroshio intrusion and eddy shedding at Luzon Strait during 1992–2001. The Kuroshio penetrates into the South China Sea and forms a bend. The Kuroshio bend varies with time, periodically shedding anticyclonic eddies. Criteria of eddy shedding are identified: 1) When the shedding event occurs, there are usually two centers of high Sea Surface Height (SSH) together with negative geostrophic vorticity in the Kuroshio Bend (KB) area. 2) Between the two centers of high SSH there usually exists positive geostrophic vorticity. These criteria have been used to determine the eddy shedding times and locations. The most frequent eddy shedding intervals are 70, 80 and 90 days. In both the winter and summer monsoon period, the most frequent locations are 119.5°E and 120°E, which means that the seasonal variation of eddy shedding location is unclear.  相似文献   

海水中一氧化氮的极谱法测定     

任春艳  张正斌  邢磊  刘春颖  刘莲生 《中国海洋大学学报(自然科学版)》2003,33(5):801-808

本文首次采用 Nafion和 Co(salen) /Nafion修饰铂电极测定海水中的 NO,对实验条件进行了选择 ,确定了最佳实验条件 ,即反应池通氮除氧 30 min,富集时间为 4min。同时用线性扫描法对海水中的 NO进行测定 ,测得海水中 NO的浓度与氧化峰电流之间有一定线性关系。对 Nafion修饰电极而言 ,线性范围 1～ 76.9μmol/L,R2 =0 .991 6,检出限为 1 μmol/L。同时对 2种方法修饰的铂电极进行了比较 ,即当 NO浓度在 1 0 -6mol/L数量级时 2种电极基本上无差别 ,而在 1 0 -7mol/L数量级时 Co(salen) /Nafion修饰电极要明显优于 Nafion修饰电极  相似文献   

CHIMEHERB人工皮使用的体会     

任永安  陈长安  刘英娥  陶谏 《中国海洋大学学报(自然科学版)》1989,(Z3)

从1989年4月～1990年10月,我科在69例病人中使用CHIMEHERB人工皮,覆盖治疗了供皮区,Ⅱ°烧伤创面、脱痂创面的残余肉芽创面.临床应用表明:CHIMEHERB人工皮能加快创面的愈合,是目前较好的一种创面覆盖物,用人工皮覆盖创面2～3天后,人工皮干燥,不需再换药,可减轻病人的痛苦.  相似文献   

Geodynamics of the deep sedimentary basin of the Caspian Sea region: paragenetic correlation of seismicity and mud volcanism     

Ibrahim?Guliev  Behrouz?PanahiEmail author 《Geo-Marine Letters》2004,24(3):169-176

In this paper we analyze seismic regime and earthquake depth distribution and correlation of seismicity and mud volcanism in the Azerbaijan and the Caspian Sea region. For the present region we have calculated accurate source locations, seismic activity, earthquake repetition and released earthquake energy parameters. It is shown that the active tectonic processes in the region are concentrated within the thick sedimentary cover that we consider as a general source of contemporary stress and a main structural element responsible for the origin of regional earthquakes. The correlation of seismicity and mud volcanism is of paragenetic character.  相似文献   

液体中光声信号吸收衰减的研究     

任新敏  戚诒让  许龙江  张德勇  周如城 《中国海洋大学学报(自然科学版)》1995,(4)

研究水的声吸收所引起的光声脉冲幅度的变化。脉冲声波由激光通过光击穿机制产生。文中给出了光声脉冲峰值声压与传播距离和声吸收系数之间的理论关系式。实验测量结果和理论曲线所描述的变化规律相符合。  相似文献   

中国花鲈细胞色素b基因片段的序列研究(英文)     

高天翔  张秀梅  陈大刚  张美昭  任一平  张亚平 《中国海洋大学学报(自然科学版)》2001,31(2)

参考鳗鲡等鱼类线粒体 DNA序列进行了中国花鲈线粒体 DNA细胞色素 b基因片断的引物设计、PCR扩增及其序列测定。得到中国花鲈的碱基序列为 4 10 bp,其 A、T、G、C含量分别为 10 1bp(2 4 .6 3% )、112 bp(2 7.32 % )、72 bp(17.56 % )、12 5bp(30 .4 9% ) ,与鳗鲡等其他鱼类相同基因片断序列碱基含量相似。  相似文献   

用Matlab中的Neural Network Toolbox仿真赤道东太平洋SST的预报模型   总被引：2，自引：0，他引：2  

张韧  蒋国荣  李妍 《海洋科学》2001,25(2):38-42

基于ＮＣＥＰ／ＮＣＡＲ再分析资料和ＣＯＡＤＳ海洋资料中的全球月平均海平面气压场、８５０hPa纬向风场及海洋温度场，利用Ｍatlab中的Ｎeural Network Toolbox仿真环境和ＢＰ模型改进算法比较准确地仿真和反演出了南方涛动指数、赤道纬向风指数和滞后的赤道东太平洋海温之间的动力结构和预报模型。该模型具有很好的拟合精度和可行的预报效果。可在一定时效内预测赤道东太平洋月平均海温的变化趋势。由于所建系统是具有直接因果关系的预报模型，因此不仅可直接用于预测，而且可有效避免类拟非线性微分方程组在积分过程中由于对初值敏感性而可能产生的对预报结果的不确定性。  相似文献   

Detection of DNA damage in mussels and sea urchins exposed to crude oil using comet assay     

Taban IC  Bechmann RK  Torgrimsen S  Baussant T  Sanni S 《Marine environmental research》2004,58(2-5):701-705

The single-cell microgel electrophoresis assay or the comet assay was used to evaluate DNA damage of dispersed crude oil on sea urchins (Strongylocentrotus droebachiensis) and mussels (Mytilus edulis L.). Sea urchins were exposed to 0.06 and 0.25 mg/L dispersed crude oil in a continuous flow system, while the mussels were exposed to 0.015, 0.06 and 0.25 mg/L dispersed crude oil. Sea urchin coelomocytes and mussel haemocytes were sampled after 4 and 5 weeks exposure, respectively. In the sea urchin coelomocytes, there was a significant concentration-related increase in the percentage of DNA in comet tail. In mussel haemocytes, there was a significantly higher percentage of DNA in comet tail for all treatments compared to the control. The responses were concentration-related up to 0.06 mg/L oil. The two highest exposure concentrations of mussels were not significantly different from each other. These results indicate that the comet assay can be used for biomonitoring of DNA damage in marine invertebrates following oil contamination.  相似文献   

Co–rich Mn crusts from the Magellan Seamount cluster: the long journey through time     

Geoffrey P. Glasby  Xiangwen Ren  Xuefa Shi  Irina A. Pulyaeva 《Geo-Marine Letters》2007,27(5):315-323

The Magellan seamounts began forming as large submarine shield volcanoes south of the equator during the Cretaceous. These volcanoes formed as a cluster on the small Pacific plate in a period when tectonic stress was absent. Thermal subsidence of the seafloor led to sinking of these volcanoes and the formation of guyots as the seamounts crossed the equatorial South Pacific (10–0°S) sequentially and ocean surface temperatures became too high for calcareous organisms to survive. Guyot formation was completed between about 59 and 45 Ma and the guyots became phosphatized at about 39–34 and 27–21 Ma. Ferromanganese crusts began formation as proto-crusts on the seamounts and guyots of the Magellan Seamount cluster towards the end of the Cretaceous up to 55 Ma after the formation of the seamounts themselves. The chemical composition of these crusts evolved over time in a series of steps in response to changes in global climate and ocean circulation. The great thickness of these crusts (up to 15–20 cm) reflects their very long period of growth. The high Co contents of the outer parts of the crusts are a consequence of the increasing deep circulation of the ocean and the resulting deepening of the oxygen minimum zone with time. Growth of the Co-rich Mn crusts in the Magellan Seamount cluster can be considered to be the culmination of a long journey through time.  相似文献