Isolation of CYP17 Ⅰ, 3β-HSD and AR Genes from Spotted Sea Bass (Lateolabrax maculatus) Testis and Their Responses to Hormones and Salinity Stimulations     

CHI Meili  NI Meng  JIA Yongyi  GU Zhimin  WEN Haishen 《中国海洋大学学报(英文版)》2019,(2):420-430

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Assessing effects of “source-sink” landscape on non-point source pollution based on cell units of a small agricultural catchment     

WANG Jin-liang  CHEN Cheng-long  NI Jiu-pai  XIE De-ti  GUO Xi  LUO Zhi-jun  ZHAO Yue 《山地科学学报》2019,(9)

Ascertaining the relationship between "source-sink" landscape and non-point source(NPS) pollution is crucial for reducing NPS pollution, however, it is not easy to realize this target on cell unit scale. To reveal the relationships between "sourcesink" landscape and NPS pollution based on cell units of a small catchment in the Three Gorges Reservoir Region(TGRR), the runoff and nutrient yields were simulated first by rainfall events on a cell unit scale based on the Annualized AGricultural Non-Point Source Pollution Model(AnnAGNPS). Landscape structure and pattern were quantified with "sourcesink" landscape indicators based on cell units including landscape area indices and locationweighted landscape indices. The results showed that:the study case of small Wangjiagou catchment highlighted a good prediction capability of runoff and nutrient export by the AnnAGNPS model. Throughout the catchment, the spatial distribution trends of four location-weighted landscape indices were similar to the trends of simulated total nitrogen(TN) and total phosphorus(TP), which highlighted the importance of spatial arrangement of "source" and "sink" landscape types in a catchment when estimating pollutant loads. Results by Pearson correlation analysis indicated that the location-weighted landscape index provided a more comprehensive account of multiple factors, and can better reflect NPS-related nutrient loss than other landscape indices applied in single-factor analysis. This study provides new findings for applying the "source-sink" landscape indices based on cell units in small catchments to explain the effect of "source-sink" landscape on nutrient export based on cell unit, and helps improve the understanding of the correlation between "source-sink" landscape and NPS pollution.  相似文献   

产新琼四糖的海洋微泡菌属AG1热稳定性β-琼胶酶的过量表达与鉴定     

朱艳冰  高贺  李鹤宾  倪辉  姜泽东  李利君  肖安风 《海洋学报(英文版)》2019,38(2):96-106

从微泡菌属AG1（Microbulbifer sp. AG1）克隆得到1302 bp大小的琼胶酶基因,该基因编码产物为一个成熟蛋白（413个氨基酸残基）外加一个信号肽（20个残基）。将不含信号肽片段的琼胶酶在E. coli BL21 （DE3）中进行了异源表达和纯化。使用琼脂糖作为底物,该重组琼胶酶的最适反应温度和pH分别为60℃和7.5。该重组酶表现出优良的热稳定性,在50℃和60℃下处理1 h,重组琼胶酶仍能分别保持67%和19%的残余酶活力。除了SDS,重组琼胶酶对于其他测试的抑制剂、去垢剂和尿素变性剂有着较好的抗性。利用薄层色谱和以对硝基苯-α/β-D-吡喃半乳糖苷为底物的酶活力分析结果表明,该重组琼胶酶为β型琼胶酶,它水解琼脂糖的主要终产物为新琼四糖,而且不同聚合度的酶解产物具有抗氧化活性。  相似文献   

北斗三号系统与GPS定位性能仿真对比分析     

倪煜淮  许杰  邹永刚  李厚朴 《海洋测绘》2019,39(4):71-74

为了解北斗三号系统在国内外及南北极地区的定位性能,在STK仿真平台下建立了北斗三号系统30颗星(3GEO、3IGSO和24MEO)的星座模型,对北京、三亚、新加坡、南北极点等11个站点的平均可见星数和几何精度因子GDOP值进行了分析,并与GPS进行了对比。结果表明,北斗三号系统较GPS在国内外及南北极地区典型站点的定位性能更优。  相似文献   

多维度动态农村土地承包经营权业务权限的设计与实现     

万术海  倪慧珠  王飞龙  刘校妍  张傲敏 《测绘与空间地理信息》2019,42(3):152-155,161

为满足浙江省各级农经管理部门在农村土地承包经营权管理系统中复杂的权限管理需要,基于RBAC模型实现了以Apache Shiro安全框架为支撑的多维度动态业务权限管理。该模型从角色维度、部门维度、空间维度、时间维度上分别对用户的资源菜单、工作流、数据操作空间、数据历史回溯4个方面的权限进行细粒度控制。在浙江省农村土地承包管理信息系统中实际运行表明,设计的业务权限系统可满足省市县各级农经管理部门在管理系统的权限要求。  相似文献   

Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype, allozyme and RAPD analyses     

YOU Feng  WANG Wei  XU Dongdong  ZHU Xiangping  NI Jing  WU Zhihao  XU Yongli  WANG Xincheng  ZHANG Peijun 《中国海洋湖沼学报》2009,27(2)

The hybrid between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus was produced by artificial insemination of olive flounder eggs with stone flounder Sperm. Sinistral and dextral are two types of hybrid progeny after metamorphosis. Karyotypes of both hybrid flounders are the same as those of the two parental species. Of the 22 loci examined from 12 allozymes,12 confirmed hybridization of the paternal and matemal loci in hybrids and no difference was found in allozyme patterns of sinistral and dextral hybrid fishes. RAPD patterns of these specimens were also studied with 38 primers selected from 104 tested. Among them, the PCR products of 30 primers showed hybridization of the paternal and maternal bands. Genetic variation between hybrids and their parental stocks was analyzed by RAPD using 10 of the above 38 primers. The average heterozygosity and genetic distance were calculated. The results suggested that the filial generation could inherit a little more genetic materials from paternal fish than that from maternal fish.  相似文献   

ETM遥感图像在措勤东部第四系含水性研究中的应用     

赵银兵  何政伟  倪忠云 《测绘科学》2009,34(5)

措勤东部属于极端缺水的地区,利用遥感技术开展第四系松散堆积物含水性调查具有重要作用。本文选用ETM753与8波段融合图像作为遥感解译底图,结合地形地貌、地质和气象影响,建立残积、冲积、洪积、冲洪积、冰川永久积雪、湖积、沼泽和坡积的识别知识体系,划定第四系松散堆积物的高、中、低、差四个含水等级,利用G IS实现第四系松散堆积物及其含水性的高程、坡度和坡向分布,为区内水资源的深入调查提供有力支持。  相似文献   

矢量图形与影像图像一体化实现   总被引：3，自引：0，他引：3  

倪建华  秦佐 《四川测绘》2009,32(3):103-105

本文在阐述影像金字塔相关理论（构建方法、影像切割等）的基础上,采用VC2005编程实现了在同一GIS系统中进行矢量数据和栅格数据叠加显示的功能模块。该模块可确定是否加载影像,并根据当前矢量图显示范围自动加载不同分辨率的影像图。  相似文献   

基于PALSAR数据的DEM提取方法研究     

倪文俭  过志峰  孙国清 《国土资源遥感》2009,(3):19-23

针对PALSAR Level 1.1数据,研究使用NASA/JPL提供的开源干涉软件包ROI_PAC Version 3.0提取DEM.ROI_PAC的目前版本只能处理Level 1.0数据,因此,文章在分析了ROI_PAC软件包处理流程的基础上,提出处理Level 1.1数据的方法,并用PALSAR Level 1.1数据对该方法做了验证.干涉重建DEM与参考DEM的对比结果表明,二者的差异均值为0.27 m,标准差为±9.24 m,80%像元点的高程误差在±10 m以内.  相似文献   

基于线段的基本线/线拓扑关系计算     

倪建华  周晓光  胡克新 《测绘软科学研究》2009,(5):39-43

空间拓扑关系是空间数据建模、空间查询、分析、推理、制图综合、数据质量控制、数据更新等的基础。目前空间拓扑关系研究的主要成果仍然集中在拓扑关系的描述方面,在计算方面的阐述都非常薄弱,不足以指导系统开发人员编程实现。本文研究了IR2中两个简单线目标间拓扑关系的计算方法,重点分析了线/线拓扑关系计算的特点,提出了一种基于线段的线/线拓扑关系计算方法,并用VC＋＋采用底层开发模式实现了基本线/线拓扑关系的计算。  相似文献