Molecular identification and culture observation on <Emphasis Type="Italic">Acrochaete leptochaete</Emphasis> (Chaetophoraceae,Chlorophyta) from China     

邓蕴彦  汤晓荣  黄冰心  滕林宏  丁兰平 《中国海洋湖沼学报》2012,30(3):476-484

Acrochaete leptochaete (Huber) Nielsen (Chaetophoraceae, Chlorophyta) was isolated from the macroalgae Chaetomorpha collected from intertidal pools in Rongcheng, Shandong, China. 18S rDNA combined with ITS regions were used to ascertain the morphological identification of the isolated material. Based on the unialgal culture, asexual reproduction and growth characteristics of A. leptochaete were investigated over wide ranges of temperature and irradiance. Results revealed that asexual reproduction of A. leptochaete could be realized by biflagellate zoospores. The zoospores germinated directly to give self- replicating generations. Zoospore germination was bipolar. A temperature range from 13-21°C and a lower irradiance of 36 μmol/(m2·s) were most favorable for the growth of A. leptochaete. Thallus organization, an important taxonomic criterion for the genus Acrochaete, was affected markedly by temperature and irradiance. Our results extend the knowledge about the species’ general biology and its morphological plasticity. For classification and identification of a simple microphytic algae like A. leptochaete, which are traditionally placed in the class Chaetophoraceae, we propose that molecular tools associated with culture observations are applied.  相似文献   

Impacts of mariculture practices on the temporal distribution of macrobenthos in Sandu Bay,South China   总被引：3，自引：0，他引：3  

周进 《中国海洋湖沼学报》2012,30(3):388-396

Sandu Bay is located in the East China Sea and is characterized by high-density fish farming and kelp culture. Despite this, little is known about the impacts of these different mariculture practices on the local environment. We investigated the temporal variation in macrobenthos and environmental conditions at three sites in the bay (fish farming site, kelp culture site, and a control site). We collected water and sediment samples during nine cruises between May 2009 and February 2010. The density of macrobenthos peaked at the fish farming site in July (655 ind./m2) whereas density did not fluctuate as widely at the other two sites. Biomass varied significantly at both the control and kelp culture sites, but had only a single peak at the fish farming site in June (21.90 g/m2). The dominant species varied throughout the study period at the control and kelp culture sites, whereas a single terebellid species (Lysilla pacifica) dominated the macrobenthos at the fish farming site. The diversity index H’ increased at the control site beginning in February then decreased after May, whereas H’ was low at the other sites in December. The mean dissolved oxygen level was highest at the control site (6.59 mg/L) and lowest at the fish farming site (5.54 mg/L). DO levels were lowest at all sites in summer (July and August). The sediment acid volatile sulfide content was higher at the fish farming site (1.46 mg/g dry weight) than those at the kelp culture and control sites (1.22 and 0.14 mg/g, respectively). Our results suggest that mariculture practices have a clear impact on the benthic environment/ community in Sandu Bay.  相似文献   

Pigment characterization for the 2011 bloom in Qinhuangdao implicated "brown tide" events in China   总被引：6，自引：0，他引：6  

孔凡洲  于仁成  张清春  颜天  周名江 《中国海洋湖沼学报》2012,30(3):361-370

A large-scale bloom occurred from May to June in 2011 in sea area near Qinhuangdao of the Bohai Sea, leading to huge damage of the scallop culture industry. Similar blooms have been observed in this region for three years. The causative species of the bloom, which dominated the phytoplankton community with the maximum cell density around 109 cell/L, could not be identified with morphological features due to the small cell size (~2 mm). A pigment analytical method was then adopted to analyze the pigment profile of the phytoplankton samples collected from the blooming sea area. It was found that pico-sized (<2 mm), nano-sized (2-20 mm), and bulk phytoplankton samples had similar pigment profile, representing the pigment signature of the bloom-causative species. The major pigments detected included 19-butanoyloxyfucoxanthin (But-fuco), fucoxanthin (Fuco), diadinoxanthin (Diad) and chlorophyll a (Chl a), and high content of But-fuco was the most significant characteristics of the phytoplankton samples. Based on the pigment composition and content, the bloom-causative species could be tentatively identified as pelagophyte, "type 8" group of haptophyte, or silicoflagellate. Some unique features of the bloom, such as the extremely high cell density, small-sized and But-fuco containing cells, occurring in early summer, and the feeding-cessation effects on scallops, suggest it be a "brown tide" event similar to those reported in the east coast of the United States of America. The recurrent "brown tide" events and their dramatic impacts on the shellfish mariculture industry in Qinhuangdao need close attention in the coming years.  相似文献   

BAC end sequencing of Pacific white shrimp <Emphasis Type="Italic">Litopenaeus vannamei</Emphasis>: a glimpse into the genome of Penaeid shrimp     

赵翠  张晓军  柳承璋  郇聘  李富花  相建海  黄超 《中国海洋湖沼学报》2012,30(3):456-470

Little is known about the genome of Pacific white shrimp (Litopenaeus vannamei). To address this, we conducted BAC (bacterial artificial chromosome) end sequencing of L. vannamei. We selected and sequenced 7 812 BAC clones from the BAC library LvHE from the two ends of the inserts by Sanger sequencing. After trimming and quality filtering, 11 279 BAC end sequences (BESs) including 4 609 paired- ends BESs were obtained. The total length of the BESs was 4 340 753 bp, representing 0.18% of the L. vannamei haploid genome. The lengths of the BESs ranged from 100 bp to 660 bp with an average length of 385 bp. Analysis of the BESs indicated that the L. vannamei genome is AT-rich and that the primary repeats patterns were simple sequence repeats (SSRs) and low complexity sequences. Dinucleotide and hexanucleotide repeats were the most common SSR types in the BESs. The most abundant transposable element was gypsy, which may contribute to the generation of the large genome size of L. vannamei. We successfully annotated 4 519 BESs by BLAST searching, including genes involved in immunity and sex determination. Our results provide an important resource for functional gene studies, map construction and integration, and complete genome assembly for this species.  相似文献   

Mineral constituents in common chanterelles and soils collected from a high mountain and lowland sites in Poland   总被引：1，自引：1，他引：0  

Jerzy FALANDYSZ  Magorzata DREWNOWSKA  Grazyna JARZY SKA 《山地科学学报》2012,9(5):697-705

This paper reported the results of the determination of Ag, Al, Ba, Ca, Cd, Co, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, P, Pb, Rb, Sr and Zn in Common Chanterelles (Cantharellus cibarius) Fr. and surface soil layer (0-10 cm) underneath the fruiting bodies. Mushrooms and soils were collected from a lowland site in the Hel Peninsula (Baltic Sea coast) and a high mountain site in the Tatra Mountains. The trace elements were determined using validated method and inductively coupled plasma - atomic emission spectroscopy (ICP-AES). Common Chanterelles that emerged at sites poor in mineral nutrients podzols of the Hel Peninsula forests efficiently bioconcentrated several essential trace elements (K, P, Co, Cu, Mn, Na, Zn), while the abundance of those elements in carpophores was around half less compared to specimens from Zakopane region and which emerged in soils much richer in minerals. Common Chanterelles collected at two spatially distant background areas in Poland were only weakly contaminated with metals such as Ag, Cd, Hg and Pb. The maximum tolerable Cd and Pb contents of certain cultivated mushrooms are regulated in the European Union by law and these hazardous metals in C. cibarius were far below tolerance limits set.  相似文献   

Arctic freshwater ostracods from modern periglacial environments in the Lena River Delta (Siberian Arctic,Russia): geochemical applications for palaeoenvironmental reconstructions     

Sebastian?WetterichEmail author  Lutz?Schirrmeister  Hanno?Meyer  Finn?Andreas?Viehberg  Andreas?Mackensen 《Journal of Paleolimnology》2008,39(4):427-449

The aim of this study is to describe ostracods from freshwater habitats in the Siberian Arctic in order to estimate the present-day relationships between the environmental setting and the geochemical properties of ostracod calcite. A special focus is on the element ratios (Mg/Ca, Sr/Ca), and the stable isotope composition (δ¹⁸O, δ¹³C), in both ambient waters and ostracod calcite. The most common species are Fabaeformiscandona pedata and F. harmsworthi with the highest frequency in all studied waters. Average partition coefficients D(Sr) of F. pedata are 0.33 ± 0.06 (1σ) in females, and 0.32 ± 0.06 (1σ) in males. A near 1:1 relationship of δ¹⁸O was found, with a mean shift of Δ_mean = 2.2‰ ± 0.5 (1σ) to heavier values in ostracod calcite of F. pedata as compared to ambient waters. The shift is not dependent on δ¹⁸O_water, and is caused by metabolic (vital) and temperature effects. Temperature-dependence is reflected in the variations of this shift. For ostracod calcite of F. pedata a vital effect as compared to inorganic calcite in equilibrium was quantified with 1.4‰. Results of this study are valuable for the palaeoenvironmental interpretation of geochemical data of fossil ostracods from permafrost deposits.  相似文献   

Synthesis of coda wave envelopes in randomly inhomogeneous elastic media in a half-space: single scattering model including Rayleigh waves     

Takuto Maeda  Haruo Sato  Takeshi Nishimura 《Geophysical Journal International》2008,172(1):130-154

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Identification of changes in heavy rainfall events in Ontario,Canada     

Yi?WangEmail author  Edward?A.?McBean  Philip?Jarrett 《Stochastic Environmental Research and Risk Assessment (SERRA)》2015,29(8):1949-1962

To assess whether changes in the frequency of heavy rainfall events are occurring over time, annual maximum records from 21 rainfall gauges in Ontario are examined using frequency analysis methods. Relative RMSE and related boxplots are used to characterize assessment for selecting distributions; the Gumbel distribution is verified as one of the most suitable distributions to provide accurate quantile estimates. Records were divided into two time periods, and tested using the Mann-Kendall test and lag-1 autocorrelations to ensure that data in each period are identically distributed. The confidence intervals of design rainfalls for each return period (2, 5, 10, and 25-year) are derived by using resampling method, and compared at 90 % confidence levels. The changes in heavy rainfall intensities are tested at gauges across the Province of Ontario. Several significant decreases in heavy rainfall intensities are identified in central and southern Ontario. Increases in heavy rainfall intensities are identified in gauges at Sioux Lookout and Belleville. The sensitivity analysis of changes identified with respect to the year of splitting indicates changes are occurring during the 1980s and 1990s.  相似文献   

Deflection amplification factor for estimating seismic lateral deformations of RC frames   总被引：1，自引：1，他引：0  

Maryam Samimifar  Asghar Vatani Oskouei  Fayaz Rahimzadeh Rofooei 《地震工程与工程振动(英文版)》2015,14(2):373-384

Different values have been assigned to the ratio of the defl ection amplifi cation factor(Cd) to the response modifi cation factor(R) for a specifi ed force-resisting system in the seismic design provisions while the same application is defi ned for it. An analytical study of the seismic responses of several reinforced concrete frames subjected to a suite of earthquake records performed in this research indicate that the stories’ overstrength and stiffness distribution along the structural height can affect local defl ections more than global ones. Therefore, the Cd/R ratio is calculated based on the ratio of both maximum inelastic to maximum elastic displacements and interstory drifts. Due to damage concentration in some specifi c stories, the defl ection amplifi cation factor calculated based on inelastic interstory drifts was larger than that of the inelastic displacements. Consequently, a minimum value of 1.0 is recommended for the Cd/R ratio in order to estimate maximum inelastic drifts. The ratio of inelastic to elastic displacement was generally found to increase slightly along the structural height for the studied RC models. In addition, it was detected that the story damage indices of the studied RC frames decrease when the inverted value of inelastic interstory drift ratios are increased through a(negative) power form.  相似文献   

Full-scale tests of wind effects on a long span roof structure     

Fu Jiyang  Zheng Qingxing  Wu Jiurong  Xu An 《地震工程与工程振动(英文版)》2015,14(2):361-372

Full-scale measurements are regarded as the most reliable method to evaluate wind effects on large buildings and structures. Some selected results are presented in this paper from the full-scale measurement of wind effects on a long-span steel roof structure during the passage of Typhoon Fanapi. Some fi eld data, including wind speed and direction, acceleration responses, etc., were continuously and simultaneously recorded during the passage of the typhoon. Comprehensive analysis of the measured data is conducted to evaluate the typhoon-generated wind characteristics and its effects on a long-span steel roof. The fi rst four natural frequencies and their vibration mode shapes of the Guangzhou International Sports Arena(GISA) roof are evaluated by the stochastic subspace identifi cation(SSI) method and comparisons with those from fi nite element(FE) analysis are made. Meanwhile, damping ratios of the roof are also identifi ed by the SSI method and compared with those identifi ed by the random decrement method; the amplitude-dependent damping behaviors are also discussed. The fullscale measurement results are further compared with the corresponding wind tunnel test results to evaluate its reliability. The results obtained from this study are valuable for academic and professional engineers involved in the design of large-span roof structures.  相似文献