利用RAPD和AFLP标记初步构建太平洋牡蛎的遗传连锁图谱   总被引：24，自引：0，他引：24       下载免费PDF全文

李莉  郭希明 《海洋与湖沼》2003,34(5):541-551

利用RAPD和AFLP标记 ,以一回交家系 [(Miyagi×Hiroshima)×Miyagi]为作图群体 ,构建了太平洋牡蛎的遗传连锁图谱。用经过筛选的 3 3个RAPD引物和 1 1个AFLP引物组合 ,对父母本和 80个子代个体进行了遗传分析。共得到母本分离标记 1 93个 ,其中 1 44个符合1∶1孟德尔分离规律。父本分离标记 1 5 6个 ,其中 1 1 1个符合 1∶1孟德尔分离规律。雌性框架图包括 99个遗传标记 ,定位在 1 2个连锁群中 ,覆盖 985 2cM ,标记间平均间隔 1 1 3cM。另外有 3个三联体 ,7个连锁对 ,图谱共覆盖 1 1 6 5 7cM。雄性框架图包括 72个遗传标记 ,分布在 8个连锁群 ,覆盖 81 1cM ,标记间平均间隔 1 2 7cM。另外有 4个三联体 ,3个连锁对 ,图谱共覆盖93 1 8cM。  相似文献   

Diagnosis of iridovirus in large yellow croaker by PCR   总被引：1，自引：0，他引：1  

Chen Xinhu  Wang Xiaowen  Wu Wenzhong 《海洋学报(英文版)》2003,22(4):635-642

A rapid and sensitive PCR-based method for the detection of the large yellow croaker iridovirus(LYCIV) is described,which involves the amplification of a 295 bp fragment of the LYCIV ATPase gene from DNA isolated from naturally infected fish spleen.Sequencing of LYCIV ATPase gene fragment showed it shared 100% nucleotide sequence homology with the corresponding region of the ATPase gene of red sea bream iridovirus(RSIV) and sea bass iridovirus(SBIV),suggesting that LYCIV was homologous with RSIV and SBIV at least in part of the gemone.The specificity and sensitivity of the PCR procedure were tested on the iridovirus-infected fishes,the expected fragment was detected from spleen DNA samples of infected fishes,whereas no fragments were amplified from healthy fish spleen DNA,white spot syndrome baculoviruses(WSBV) DNA and pseudorabies virus(PRV) DNA.Detection limit of this method was 10^-7 ng positive plasmid DNA containing target sequence,equal to about 100 virions.In the infected experiment,first positive detection(1/4) appeared at Day 3 post-infection,all fish(4/4) tested positive at Day 7,however obvious symptoms were observed at Day 8,so LYCIV infection could be detected prior to the appearance of obvious symptoms.These results indicate that this PCR method could be used for early,rapid and specific detection of LYCIV infection.  相似文献   

雌雄比对海带雌配子体发育的影响     

罗世菊  丛沂周  杨迎霞  张全胜  李晓捷  朱龙  张壮志 《海洋湖沼通报》2005,(1):33-36

本实验进行了不同雌雄比对海带雌配子体发育的影响。结果表明：雌雄比(鲜重)为1：1及2：1，实验组出现排卵时间最早，且发育率高，实验第20天，发育率超过70％，孢子体正常。5：1，10：1，20：1实验组随着雄性比例的减少，发育率逐渐降低，随着培养天数的增加，三个实验组均出现未受精卵及畸形孢子体，雌雄比越大，未受精卵数目越多。  相似文献   

In-Situ Observation of Fluid Mud in the North Passage of Yangtze Estuary, China   总被引：1，自引：0，他引：1  

李九发  万新宁何青应铭  时连强S.M.Hutchinson 《中国海洋工程》2004,18(1):149-156

Observations of fluid mud were made in the lower North Passage of the Yangtze Estuary in February 2000, on 10 -11 August 2000, on 30 - 31 August 2000 (after two strong typhoons), on 21 - 24 August 2000 (neap tide) and on 3 -6 September 2000 (mean tide) respectively. In situ data show that the fluid mud in this area consists of fine cohesive sediment (median size 7.23 μm). The formation and movement of fluid mud varied during the neap-spring and flood-ebb tidal cycle. Observations suggest that fluid mud phenomena in this area may be categorised in a three-fold manner as slack water, storm and saltwedge features. The thickness of the fluid mud layer of slack water during the neap tide ranged from 0.2 to 0.96 m, whereas during the mean tide, the thickness ranged from 0.17 to 0.73 m, and the thickness of the fluid mud layer was larger during slack water than at the flood peak. Shoals cover an area of 800 km^2 with a water depth smaller than 5 m. Erosion of these extensive intertidal mudflats due to storm action provides an abundant sediment source. This is particularly significant in this estuary when the tidal level is lower than 5 m. The lower North Passage is a typical zone of saltwater wedging, so the saltwedge fluid mud has the most extensive spatial range in the estuary.  相似文献   

海洋重力测量数据处理系统MGDPS及其应用     

杨金玉  张训华  张志珣  杨慧良 《中国海洋大学学报(自然科学版)》2007,37(1):116-120

利用Delphi结合MapX开发的可视化船测海洋重力测量数据处理软件系统MGDPS,从专业角度详细论述了软件各模块的功能。使用实测数据对该系统进行了测试,实际应用表明,该系统具有准确可靠,易掌握,方便使用等特点,对我国海洋重力测量具有重要意义和使用价值。  相似文献   

黄河三角洲埕岛海域浅地层剖面结构与灾害地质     

李俊杰  李广雪  文世鹏  杨荣民  郝坤 《海洋地质前沿》2007,23(12):8-13

通过钻孔和高分辨率浅地层剖面的综合对比,对黄河三角洲北部与埕岛油田海底工程密切相关的浅地层剖面结构进行细分,划分出末次冰期低海面以来的5套沉积相层和6个地震相层.分析表明,本区广泛分布的地质灾害是由其特殊的沉积结构和复杂的动力环境造成的,尤其是工程软弱层的存在造成三角洲前缘的不稳定性.  相似文献   

双壳类软体动物精子发生及其在系统演化研究中的应用前景   总被引：11，自引：0，他引：11  

竺俊全  杨万喜 《海洋湖沼通报》2002,(4):25-31

综述了双壳类软体动物精子发生及精子形态结构方面的研究进展，指出双壳类软体动物精子发生过程中精细胞分化具有种、属特异性，精细胞分化的细节是区别个体外部形态很近的物种的依据；精子的形态结构具有种的特征，精子结构的比较研究将成为双壳类软体动物系统演化研究的重要方法之一。  相似文献   

Sediment transport pattern at the Barra Nova inlet, south Portugal: a conceptual model     

Yann Balouin  Hélène Howa 《Geo-Marine Letters》2002,21(4):226-235

The Barra Nova inlet, in south Portugal, is known to migrate progressively southeastwards under wave action. The morphodynamics of this system during a representative year suggests that this long-term evolution is dependent on a seasonal behavior of the tidal inlet which can be described through a three-stage model of post-storm, transition and extended fair-weather conditions. Processes involved in this evolution indicate that the historical migration of the Barra Nova is not dependent on the longshore drift forcing constricting the channel on the updrift coast, but rather on the adjustment of the system to a major erosion of the downdrift coast during short storm events.  相似文献   

冲绳海槽轴线地质特征   总被引：10，自引：2，他引：10  

杨文达  王振宇  曾久岭 《海洋地质与第四纪地质》2001,21(2):1-6

冲绳海槽位于东海大陆架与琉球岛弧之间，海槽中发育着一系列平行轴向延伸的地堑型正断层。穿越海槽的地震剖面证实了海槽扩张中心的存在，扩张轴沿海槽轴线方向延伸。由于地堑构造中心的扩张速率较大，由某引起的海槽中心下陷的速率大于沉积作用的补偿速率，使海槽中心海底出现明显的下陷。在轴线的大陆坡一侧，陆源沉积占绝对优势；轴线的岛坡一侧，以火山坡碎屑、浮岩、有孔虫软泥为沉积特征。  相似文献   

耳鲍(Haliotis asinina)核糖体小亚基(18S rRNA)编码基因的克隆与序列分析   总被引：1，自引：0，他引：1       下载免费PDF全文

黄勃  方再光  刘均玲  周智  王小兵 《海洋与湖沼》2007,38(3):212-216

采用分子生物学的方法,对南海不同海区的两个地理群体耳鲍(Haliotis asinina)18S rRNA基因全长进行了克隆和序列分析,并将耳鲍18S rRNA基因的序列与NCBI数据库中已收录鲍的18SrRNA基因进行了比较。结果发现,南海耳鲍核糖体18S rRNA基因与耳鲍H.asinina isolate H11核糖体18S rRNA基因序列的相同率高达98%;同一地理群体内耳鲍核糖体18S rRNA基因序列完全一致;不同地理群体间耳鲍核糖体18S rRNA基因在碱基组成上的相似率为99%,仅在某些位点处发生了碱基替换,即腺嘌呤(T)被鸟嘌呤(G)替换;同时,将这两个不同群体中耳鲍的18S rRNA基因与泰国耳鲍18S rRNA基因序列进行比较分析发现,它们之间也只是发生了碱基替换。  相似文献