Molecular analysis of complete ssu to lsu rdna sequence in the harmful dinoflagellatealexandrium tamarense (korean isolate, HY970328M)     

Jang-Seu Ki  Myung-Soo Han 《Ocean Science Journal》2005,40(3):43-54

New PCR primers (N=18) were designed for the isolation of complete SSU to LSU rDNA sequences from the dinoflagellateAlexandrium tamarense. Standard PCR, employing each primer set selected for amplifications of less than 1.5 kb, successfully amplified the expected rDNA regions of A. tamarense (Korean isolate, HY970328M). Complete SSU, LSU rDNAs and ITS sequences, including 5.8S rDNA, were recorded at 1,800 bp, 520 bp and 3,393 bp, respectively. The LSU rDNA sequence was the first report inAlexandrium genus. No intron was found in the LSU rRNA coding region. Twelve D-domains within the LSU rDNA were put together into 1,879 bp (44.4% G+C), and cores into 1514 bp (42.8% G+C). The core sequence was significantly different (0.0867 of genetic distance, 91% sequence similarity) in comparison withProrocentrum micans (GenBank access. no. X16108). The D2 region was the longest in length (300 bp) and highly variable among the 12 D-domains. In a phylogenetic analysis using complete LSU rDNA sequences of a variety of phytoplankton,A tamarense was clearly separated with high resolution against other species. The result suggests that the sequence may resolve the taxonomic ambiguities ofAlexandrium genus, particularly of the tamarensis complex.  相似文献   

白沙口潮汐电站前海区泥沙冲淤规律及防淤对策的研究     

林宝荣  杨光复  李凡  周天成  韩有松  张铭汉 《海洋科学集刊》1990,(31):1-24

白沙口潮汐电站的淤沙问题在1972年就由中国科学院海洋研究所与山东省水文总站合作进行了研究,并于1974年提出了研究结果和淤沙防治措施的建议。但由于某些原因有关建议未能得以实施。近几年来淤沙又有发展,严重威胁着潮汐电站(图1)的寿命。1983年9月国家科委海洋专业组与山东省科委决定对白沙口电站的淤沙问题开展进一步的研究。解决洄淤问题,不仅是白沙口潮汐电站的迫切任务,而且对开发我国潮汐资源也有着重要的意义。本文根据此项研究的成果撰写,主要阐明电站泥沙运动的基本规律,在此基础上提出防治措施的建议。  相似文献   

东海大规模赤潮危害影响的实验研究——对黑褐新糠虾和卤虫的毒性效应     

颜天  张艺  韩刚  陈洋  周名江 《海洋科学集刊》2007,(48):166-175

The effects of Prorocentrum donghaiense,Karenia mikimotoi,Alexandrium catenella,the causative species of Harmful Algal Blooms in the East China Sea,on the survival and growth of Neomysis awatschensis and Artemia salina were investigated.And the toxicity of eleven strains of Alexandrium----A.lusitanicum(AL-1)、A.minutum(AM-1)、A.spp.(A-K)、A.tamarense(ATHK,ATCI02,ATCI03,AT5-1,AT5-3,AT-6)、A.affine(AS-1,AC-1) to the two above organisms were compared. The results showed that P.donghaiense and K.mikimotoi had no adverse effect on the survival and growth of N.awatschensis and A.salina at 96h.However,the survival rate of N.awatschensis and A.salina declined linearly with the increasing density of A.catenella,and the 96h LC₅₀ for N.awatschensis and A.salina was 4500 and 460 cells/mL,respectively, Five strains of Alexandrium----A.spp,A.lusitanicum (AL-1),A.tamarense(AT5-3,AT-6) and A.affine (AS-1)at the density of 4000cells/mL and 96h had no adverse impacts on N.awatschensis. But other six strains of Alexandrium a.Tamarense(AT5-1,ATHK,ATCI02,ATCI03),A.affine(AC-1) and A.minutum(AM-1)decreased the survival fate of N.awatschensis significantly. Two strains of Alexandrium----A.lusitanicum(AL-1) and A.tamarense(AT-6),at the density of 500cells/mL. had no adverse effects on A.salina in 96h;while the other Alexandrium strains all had adverse effeets on the survival of a.salina, A.salina could increase its length with the increasing density of P.donghaiense,K.mikimotoi and A.luxitanicum. The results indicate that P.donghaiense and K.mikimotoi have nio significant effect on the survival of crustaeean zoop lankton in a short period. But several Alexandrium specised/strains could influence the survival of crustacean zooplankton likely by non-PSP substances. A.salina is shown to be more sensitive than N.awatschensis as test organisms to harmful species.  相似文献   

Changes in the hydrography of Central California waters associated with the 1997–98 El Niño     

C. A. Collins  C. G. Castro  H. Asanuma  T. A. Rago  S. -K. Han  R. Durazo  F. P. Chavez 《Progress in Oceanography》2002,54(1-4)

Oceanographic conditions off Central California were monitored by means of a series of 13 hydrographic cruises between February 1997 and January 1999, which measured water properties along an oceanographic section perpendicular to the California Coast. The 1997–98 El Niño event was defined by higher than normal sea levels at Monterey, which began in June 1997, peaked in November 1997, and returned to normal in March 1998. The warming took place in two distinct periods. During June and July 1997, the sea level increased as a result of stronger than normal coastal warming below 200 dbars and within 100 km of the coast, which was associated with poleward flow of saltier waters. During this period, deeper (400–1000 dbar) waters between 150–200 km from shore were also warmed and became more saline. Subsequently, sea level continued to rise through January 1998, mostly as a result of the warming above 200 dbars although, after a brief period of cooling in September 1997, waters below 200 dbar were also warmer than normal during this period. This winter warm anomaly was also coastally trapped, extending 200 km from shore and was accompanied by cooler and fresher water in the offshore California current. In March and April 1998, sea level dropped quickly to normal levels and inshore waters were fresher and warmer than the previous spring and flowed southward.The warming was consistent with equatorial forcing of Central California waters via propagation of Kelvin or coastally-trapped waves. The observed change in heat content associated with the 1997–98 El Niño was the same as that observed during the previous seasonal cycle. The warming and freshening events were similar to events observed during the 1957–58 and 1982–83 El Niños.  相似文献   

3D gravity modelling for Anyongbok Seamount in the East Sea     

Moo Hee Kang  Hyun-Chul Han  Hyesu Yun  Gee Soo Kong  Kyong O. Kim  Youn Soo Lee 《Marine Geophysical Researches》2007,28(3):257-269

A seamount chain with an approximately WNW trend is observed in the northeastern Ulleung Basin. It has been argued that these seamounts, including two islands called Ulleung and Dok islands, were formed by a hotspot process or by ridge related volcanism. Many geological and geophysical studies have been done for all the seamounts and islands in the chain except Anyongbok Seamount, which is close to the proposed spreading ridge. We first report morphological characteristics, sediment distribution patterns, and the crustal thickness of Anyongbok Seamount using multibeam bathymetry data, seismic reflection profiles, and 3D gravity modeling. The morphology of Anyongbok Seamount shows a cone shaped feature and is characterized by the development of many flank cones and flank rift zones. The estimated surface volume is about 60 km³, and implies that the seamount is smaller than the other seamounts in the chain. No sediments have been observed on the seamount except the lower slope, which is covered by more than 1,000 m of strata. The crustal structure obtained from a 3D gravity modeling (GFR = 3.11, SD 3.82 = mGal) suggests that the seamount was formed around the boundary of the Ulleung Plateau and the Ulleung Basin, and the estimated crustal thickness is about 20 km, which is a little thicker than other nearby seamounts distributed along the northeastern boundary of the Ulleung Basin. This significant crustal thickness also implies that Anyongbok Seamount might not be related to ridge volcanism.  相似文献   

太平洋波高分布及变化规律研究   总被引：4，自引：0，他引：4  

韩树宗  朱大勇  郭佩芳 《中国海洋大学学报(自然科学版)》2003,33(6):825-832

使用 Topex/ Poseidon卫星高度计 1 992年 1 0月～ 1 998年 1 2月连续 75个月 ,2 30个重复周期的有效波高资料对南北太平洋的有效波高进行了统计 ,分析了太平洋有效波高的多年平均、多年各月平均和多年各季平均的空间分布特征和时间变化规律。结果表明 ,太平洋波高分布具有明显季节变化的规律 ,与太平洋的风速分布特征具有良好的对应关系  相似文献   

High Frequency Current Fluctuations and Cross-shelf Flows around the Pycnocline near the Shelf Break in the East China Sea     

In-Seong Han  Koichiro Kamio  Takeshi Matsuno  Atsuyoshi Manda  Atsuhiko Isobe 《Journal of Oceanography》2001,57(2):235-249

Relation between internal waves with short time scale and density distribution near the shelf break in the East China Sea is studied utilizing moored current meters, thermometers and conductivity-temperature-depth (CTD) casts. A well developed pycnocline was frequently observed around 150–200 m depth near the shelf break accompanied with the development of internal waves with short time scale. During the cruise in May 1998, the intensified internal wave motion with short time scale and the distinct offshore flow were observed just below the lower pycnocline, which shoaled and extended above the shelf area. It is suggested that vertical mixing generated by amplified internal waves would produce cross-shelf ageostophic density current around the pycnocline. During the cruise in May 1999, on the other hand, the lower pycnocline was located offshore below the shelf break, and the internal wave motion was amplified just above the lower pycnocline. In this case, the offshore flow should be generated above the lower pycnocline, but vertical profiles of current velocity were not obtained because acoustic Doppler current profiler (ADCP) data were not available around the lower pycnocline.  相似文献   

莱州湾东部滨海水域砂金分布特征   总被引：1，自引：0，他引：1  

谭启新  姜玉池 《海洋地质与第四纪地质》1996,16(4):79-86

通过对莱州湾东部滨海水域地球物理和地质钻综合调查，业已查明该海区地质的构造和古地理环境特征，并发现区内砂金分布较普遍，本文从砂金分布的地貌单元，沉积物类型，砂金成因类型，物质来源，古气候，构造作用，砂金成因等诸方面，研究了该海域内砂金特征。  相似文献   

一种污水排海的近远区计算与控制方法──以大亚湾水容量计算及污水排海为例     

韩保新  彭海君  曾小清  胡康萍  吕先林 《热带海洋学报》1996,(4)

针对点容量计算中混合区限制的要求，采用了动量积分法、经验公式法和有限差分法，分别对污水排放后可能引起的近区、过渡区和远区的污水浓度变化进行了模拟预测，并就一定的限制性条件对容量点近区、高浓度混合区及容量点远区影响范围进行了分析。在大亚湾水容量计算及污水排海规划中的应用表明，该法具有计算机时较省、精度相对较高等优点。  相似文献   

晚更新世末期东亚季风活动与陆架区沉积环境变迁     

韩德亮  于洪军 《中国海洋大学学报(自然科学版)》2001,31(6):911-916

从距今 18 0 0 0年的最后冰期最盛时期到距今 10 0 0 0年开始的全新世海侵 ,全球气侯发生过急剧的变化。位于亚洲东部的古季风活动也经历了盛衰变化。大量的钻孔及浅地层剖面分析资料已经证实 ,冰期最盛时期陆架区古季风活动加强 ,陆架出现了沙漠环境与黄土沉积。当冰期结束 ,气候转暖 ,冬季风减弱之际 ,南黄海陆架及长江三角洲一带的沙漠与黄土分布范围也随之缩小 ,原先的沙漠活动区变成了黄土分布区 ,也就是所谓硬粘土沉积区。随着古季风活动的进一步衰退和海面的不断升高 ,来自陆架区的东北风变为冷湿气流。长江三角洲地区和陆架上出现了有利于沼泽发育的环境 ,全新世海侵把泥炭层淹没。陆架区晚更新世末期以来的地层中出现沙层、硬粘土和泥炭层的沉积序列 ,即“三元沉积结构”,是古冬季风盛衰过程的最好记录  相似文献