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31.
Plaice, flounder and sand goby were exposed to ethynylestradiol (EE2) for 21 days and then followed for up to 31 days after removal of the oestrogen. Plasma vitellogenin (VTG) and hepatic VTG mRNA were determined in groups of fish sampled during the induction and post-exposure phases. VTG mRNA increased slightly earlier than plasma protein, but both reached maxima by 21 days. In contrast, VTG mRNA decayed much more rapidly than protein after EE2 exposure was terminated (typical values t(1/2) mRNA 3 days, protein 15-30 days). Vitellogenin and VTG mRNA thus measure different temporal events and this is illustrated by field data of male flounder in which both parameters have been determined. Few fish show co-ordinate increased VTG mRNA and vitellogenin but rather more fish have increased vitellogenin. Low level increases of VTG mRNA (5 X) is observed in some fish without increased vitellogenin and this may represent polymorphic differences between individual fish.  相似文献   
32.
The objective of this study was to validate the outcomes of a modified decision tree classifier by comparing the produced landslide susceptibility map and the actual landslide occurrence, in an area of intensive landslide manifestation, in Xanthi Perfection, Greece. The values that concerned eight landslide conditioning factors for 163 landslides and 163 non-landslide locations were extracted by using advanced spatial GIS functions. Lithological units, elevation, slope angle, slope aspect, distance from tectonic features, distance from hydrographic network, distance from geological boundaries and distance from road network were among the eight landslide conditioning factors that were included in the landslide database used in the training phase. In the present study, landslide and non-landslide locations were randomly divided into two subsets: 80 % of the data (260 instances) were used for training and 20 % of the data (66 instances) for validating the developed classifier. The outcome of the decision tree classifier was a set of rules that expressed the relationship between landslide conditioning factors and the actual landslide occurrence. The landslide susceptibility belief values were obtained by applying a statistical method, the certainty factor method, and by measuring the belief in each rule that the decision tree classifier produced, transforming the discrete type of result into a continuous value that enabled the generation of a landslide susceptibility belief map. In total, four landslide susceptibility maps were produced using the certainty factor method, the Iterative Dichotomizer version 3 algorithm, the J48 algorithm and the modified Iterative Dichotomizer version 3 model in order to evaluate the performance of the developed classifier. The validation results showed that area under the ROC curves for the models varied from 0.7936 to 0.8397 for success rate curve and 0.7766 to 0.8035 for prediction rate curves, respectively. The success rate and prediction curves showed that the modified Iterative Dichotomizer version 3 model had a slightly higher performance with 0.8397 and 0.8035, respectively. From the outcomes of the study, it was induced that the developed modified decision tree classifier could be efficiently used for landslide susceptibility analysis and in general might be used for classification and estimation purposes in spatial predictive models.  相似文献   
33.
DIAS (European Digital Upper Atmosphere Server) effective sunspot number — R12eff was recently introduced as a proxy of the ionospheric conditions over Europe for regional ionospheric mapping purposes. Although a pre-processing step for the real-time update of the Simplified Ionospheric Regional Model (SIRM) to real-time conditions, R12eff is available in real time by DIAS system (http://dias.space.noa.gr) for independent operational use. In this paper we discuss the efficiency of R12eff to specify ionospheric conditions over Europe. For this purpose, the diurnal R12eff’s reference pattern was determined on monthly basis and for different solar cycle phases. The deviation of the real-time R12eff estimates from the reference values, ΔR12eff was found to be highly correlated with the foF2 storm-time disturbances, especially during large scale effects indicating that DIAS-R12eff can provide a reliable estimator of the ionospheric activity level over a substantial part of Europe and a powerful tool for ionospheric specification applications.  相似文献   
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Effects of nanoparticles on aquatic organisms have been little studied to date and toxicological data are urgently needed for development of regulatory frameworks for these substances. Here, we report the findings of a study exposing sticklebacks to cadmium sulfide (CdS) as bulk material and quantum dots. Fish were exposed for 21 d in a flow through test system to 5, 50 or 500 microg l(-1) CdS nanoparticles (nCdS) coated in thiol terminated methyl polyethylene glycol (MPEG), bulk CdS or MPEG at 500 microg l(-1) (nominal concentrations). With the exception of the highest nCdS exposure, measured concentrations were approximately one order of magnitude below nominal. A single fish from each group (excluding MPEG) was examined using energy dispersive X-ray analysis (EDX) to localise cadmium, however, cadmium could not be detected in whole body sections. Elevated levels of oxidized glutathione were measured in the gills of fish exposed to 50 and 500 microg l(-1) nCdS. Induction of vitellogenin synthesis was not detected in any of the treatment groups. The number of males engaged in nest-building behaviour following exposure to 500 microg l(-1) nCdS was reduced and livers of 4/6 fish in the same treatment displayed hepatocellular nuclear pleomorphism. The results are discussed emphasising the fundamental importance of experimental design and the need to understand the behaviour of nanoparticles in the aqueous phase.  相似文献   
36.
The main purpose of this study is to define the main variables that contribute to the occurrence of landslides in Kimi, Euboea, Greece, and to produce a landslide susceptibility map using the weight of evidence method. For the developed model, a sensitivity analysis is carried out in order to understand the model’s behavior when small changes are introduced in the weight value of the landslide-related variables. Landslide locations were identified from field surveys and interpretation of aerial photographs which resulted in the construction of an inventory map with 132 landslide events, while eight contributing variables were identified and exploited. All landslide-related variables were converted into a 5?×?5-m float-type raster file. These input-raster layers included a lithological unit layer, an elevation layer, a slope angle layer, a slope aspect layer, a distance from tectonic features layer, a distance from hydrographic network layer, a topographic wetness index layer, and a curvature layer. The validation of the developed model was achieved by using a subset of unprocessed landslide data, showing a satisfactory agreement between the expected and existing landslide susceptibility level, with the area under the predictive rate curve estimated to be 0.808. The area under the success rate curve was estimated to be 0.828 indicating a very high classification rate for existing landslide areas. According to the results of the sensitivity analysis, the lithological unit “yellowish gray to white marls” was the most sensitive as it had the highest change in the relative frequency of observed landslides. The overall outcomes of the performed analysis provide crucial knowledge in successful land use planning and management practice and also in risk reduction projects.  相似文献   
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Two bulk samples of clayey diatomite of Upper Miocene age originated from Western Macedonia, northern Greece and Thessaly central Greece were examined for their efficiency to be used as industrial absorbents. The samples were characterized using X-Ray Diffraction, Thermo-Gravimetric and Fourier Transform Spectroscopy, Scanning Electron Microscopy and ICP-MS analytical methods. The absorption capability of the clayey samples in oil and water were also examined. The mineralogy of both samples is predominated by the presence of clay minerals and amorphous silica. The clay minerals prevailed in the Klidi (KL) bulk sample, with muscovite being the dominant phase, and kaolinite and chlorite occurring in minor amounts. In the Drimos (DR) bulk sample, vermiculite was the predominant clay phase. Smectite was not found in either sample, whereas detrital quartz and feldspars were present in significant amounts. The amorphous silica phase (opal-A) occurs mainly with the form of disck-shaped diatom frustules. The chemistry of the samples is characterized by the predominance of silica, alumina, and iron, whereas all the other major and the trace elements are in low concentrations. Both clayey diatomite rocks exhibited sufficiently good oil and water absorption capacity, ranging between 70 to 79% in the clay-rich sample KL and 64 to 70% in the opal-A-rich sample DR. Comparing the properties of the rocks studied with other commercial absorbents, it is concluded that they may find applications as absorbents in industrial uses.  相似文献   
39.
We present an analysis of the inter-annual variability of hydrography—temperature and salinity – and ice conditions at the coastal site of Tvärminne, Gulf of Finland, Baltic Sea during the period 1927–2012. The aim is to analyze the natural variability and trends of the hydrographic characteristics, freezing and breakup dates, ice thickness, and the heat content during the last century. The study also includes the inter-connections between the ice season and the surface temperature and salinity in the open water season. The results showed a significant decrease of the ice season length, by almost 30 days. The maximum annual ice thickness decreased by 8 cm in the last 40 years. The surface water temperature increased by almost 1 °C and there was also an increasing trend in the heat content. The thermal memory of the system was 2–2.5 months. The surface salinity increased by 0.5 psu in the last 85 years. The results are discussed and compared to related studies.  相似文献   
40.
Distributions and carbon isotopic compositions of microbial lipid biomarkers were investigated in sediment cores from the G11 and G12 pockmarks in the Nyegga sector of the Storegga Slide on the mid-Norwegian margin to explore differences in depth zonation, type and carbon assimilation mode of anaerobic methane-oxidizing archaea (ANMEs) and associated sulphate-reducing bacteria responsible for anaerobic oxidation of methane (AOM) in these cold seep environments. While the G11 site is characterised by black reduced sediments colonized by gastropods and Siboglinidae tubeworms, the G12 site has black reduced sediments devoid of fauna but surrounded by a peripheral occurrence of gastropods and white filamentous microbial mats. At both sites, bulk sediments contained abundant archaeal and bacterial lipid biomarkers substantially depleted in 13C, consisting mainly of isoprenoidal hydrocarbons and dialkyl glycerol diethers, fatty acids and non-isoprenoidal monoalkylglycerol ethers. At the G11 site, down-core profiles revealed that lipid biomarkers were in maximum abundance from 10 cm depth to the core bottom at 16 cm depth, associated with δ13C values of ?57 to ?136‰. At the G12 site, by contrast, lipid biomarkers were in high abundance in the upper 5 cm sediment layer, associated with δ13C values of ?43 to ?133‰. This suggests that, as expected from the benthic fauna characteristics of the sites, AOM takes place mainly at depth in the G11 pockmark but just below the seafloor in the G12 pockmark. These patterns can be explained largely by variable fluid flow rates. Furthermore, at both sites, a dominance of ANME-2 archaea accompanied by their bacterial partners is inferred based on lipid biomarker distributions and carbon isotope signatures, which is in agreement with recently published DNA analyses for the G11 pockmark. However, the present data reveal high discrepancies in the contents and δ13C values for both archaeal and bacterial lipid profiles, implying the possible involvement of at least two distinct AOM-related microbial consortia at the inferred AOM depth zonation of G11 and G12 pockmark sediments. In both sediment cores, the δ13C profiles for most archaeal lipids suggest a direct assimilation of dissolved inorganic carbon (DIC) in addition to methane by ANMEs (chemoautotrophy); constant and highly depleted δ13C profiles for PMI:3, an archaeal lipid biomarker presumably related to ANME-2, suggest a direct assimilation of 13C-depleted methane-derived carbon via AOM (methanotrophy). Evidently, the common approach of investigating lipid biomarker contents and δ13C signatures in cold seep sediments does not suffice to precisely discriminate between the carbon assimilation mode for each ANME archaeal group and associated bacteria. Rather, this needs to be combined with further specific labelling studies including different carbon sources (methane carbon, methane-derived organic intermediates and DIC) in order to unravel the metabolic pathways of each microbial consortium involved in AOM (ANME-1 vs. ANME-2 vs. ANME-3 archaeal group and associated bacteria).  相似文献   
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