Cloning of cytochrome P450 1A (CYP1A) genes from the hermaphrodite fish Rivulus marmoratus and the Japanese medaka Oryzias latipes     

Kim IC  Kim YJ  Yoon YD  Kawamura S  Lee YS  Lee JS 《Marine environmental research》2004,58(2-5):125-129

To use two small fish Rivulus marmoratus (Cyprinodontiformes, Rivulidae) and the Japanese medaka Oryzias latipes (Belloniformes) as testing models in molecular ecotoxicology, we have cloned the cytochrome P450 1A (CYP1A) gene after screening of both genomic DNA libraries, and sequenced 11,863 and 7,243 bp including all the exons and introns with promoter regions, respectively. The Rivulus and the medaka CYP1A gene consisted of seven exons (including non-coding exons) with high homology to mammals. In the promoter region, Rivulus CYP1A gene has seven xenobiotic response elements (XREs) and two metal response elements (MREs), while the Japanese medaka CYP1A gene has six XREs and four MREs. Interestingly, medaka CYP1A gene has a number of MREs at the promoter, which may affect its response on metal exposure. We describe here the gene structure of both fish CYP1A genes.  相似文献   

Freely floating-body simulation by a 2D fully nonlinear numerical wave tank     

Weoncheol Koo  Moo-Hyun Kim 《Ocean Engineering》2004,31(16):221-2046

Nonlinear interactions between large waves and freely floating bodies are investigated by a 2D fully nonlinear numerical wave tank (NWT). The fully nonlinear 2D NWT is developed based on the potential theory, MEL/material-node time-marching approach, and boundary element method (BEM). A robust and stable 4th-order Runge–Kutta fully updated time-integration scheme is used with regriding (every time step) and smoothing (every five steps). A special φ_n-η type numerical beach on the free surface is developed to minimize wave reflection from end-wall and wave maker. The acceleration-potential formulation and direct mode-decomposition method are used for calculating the time derivative of velocity potential. The indirect mode-decomposition method is also independently developed for cross-checking. The present fully nonlinear simulations for a 2D freely floating barge are compared with the corresponding linear results, Nojiri and Murayama’s (Trans. West-Jpn. Soc. Nav. Archit. 51 (1975)) experimental results, and Tanizawa and Minami’s (Abstract for the 6th Symposium on Nonlinear and Free-surface Flow, 1998) fully nonlinear simulation results. It is shown that the fully nonlinear results converge to the corresponding linear results as incident wave heights decrease. A noticeable discrepancy between linear and fully nonlinear simulations is observed near the resonance area, where the second and third harmonic sway forces are even bigger than the first harmonic component causing highly nonlinear features in sway time series. The surprisingly large second harmonic heave forces in short waves are also successfully reproduced. The fully updated time-marching scheme is found to be much more robust than the frozen-coefficient method in fully nonlinear simulations with floating bodies. To compare the role of free-surface and body-surface nonlinearities, the body-nonlinear-only case with linearized free-surface condition was separately developed and simulated.  相似文献   

合浦珠母贝26S蛋白酶体S4,S7亚基基因片段的分离与分析     

姜铁民  张勇  黄洁  谢莉萍  吴移谋  张荣庆 《海洋科学》2003,27(4):28-34

26S蛋白酶体是真核生物中一种具有ATP依赖性的蛋白酶复合体,主要通过泛肽途径选择性降解细胞内与代谢调控、细胞周期有关的功能蛋白及异常蛋白,参与多种细胞活动的调控过程。26S蛋白酶体由具有催化活性的20S亚复合体和一个具有调节作用的19S亚复合体组成,其中19S亚复合体中的ATP酶亚基是调节26S蛋白酶体活性的重要组件。本通过简并引物PCR手段,从软体动物合浦珠母贝(Pinctada fucata)中扩增到参与构成19S亚复合体的S4和S7(MSS1)两个亚基的基因片段。这两个基因片段所编码的ATP酶组件包含有Gx4GKT,DEID,SAT和H／QRxGRxxR等26S蛋白酶体ATP酶亚基的共同功能基序。这是首次在软体动物中报道26S蛋白酶体的ATP酶亚基基因序列,为研究软体动物中26S蛋白酶体的结构与功能奠定了分子基础。  相似文献   

基于非期望产出的中国滨海旅游生态效率评价——以我国沿海城市为例     

狄乾斌  赵晓曼  王敏 《海洋通报》2020,39(2):160-168

旅游生态效率是反映旅游经济与旅游环境双向效应(bi-effect)的重要判断指标。本文基于非期望产出视角,采用超效率SBM模型,评价了我国东部沿海18个主要滨海旅游城市的旅游生态效率,并分析了其时空演变特征。研究结果表明:(1)中国东部沿海18市滨海旅游生态效率整体呈中等水平,平均值处于0.727～0.894,阶段特征明显,呈现前期平稳发展,中期下降,后期回升的发展趋势;(2)各城市滨海旅游生态效率水平差异显著,分为下行风险型,持续成长型,稳定型和波动型4种发展类型。平均效率值最高为天津,最低为三亚;(3)分解效率均值中纯技术效率较高,规模效率次之,技术进步是影响综合效率的主要因素;(4)投入冗余和非期望产出冗余已经成为阻碍滨海旅游生态效率进步的关键因素。  相似文献   

Some stability properties of large-scale baroclinic flows with nonlinear zonal current profile     

Jae Min Hyun 《Journal of Oceanography》1977,33(2):55-60

The stability properties of a baroclinic zonal current with nonlinear velocity profile are investigated. The integral method is applied to the governing eigenvalue equation having the vertical velocity as the dependent variable. Expressed in terms of the Rossby number and the Richardson number, stability criteria, unstable regions in the complexc plane, and the upper bound of the unstable wave growth rate are found. Some differences in the results are noted between the present model and the quasi-geostrophic streamfunction model, particularly in connection with the effect of the velocity profile curvature termU _zz . It is conjectured in the present model that, depending on extreme behaviors ofU _zz , the propagation speed of unstable waves can be greater thanU _max or smaller thanU _min .  相似文献   

Solution of an unstable axisymmetric Cauchy–Poisson problem of dispersive water waves for a spectrum with compact support     

T.S. Jang  S.H. Kwon  B.J. Kim 《Ocean Engineering》2007,34(5-6):676-684

 It has been known that the axisymmetric Cauchy–Poisson problem for dispersive water waves is well posed in the sense of stability. Thereby time evolution solutions of wave propagation depend continuously on initial conditions. However, in this paper, it is demonstrated that the axisymmetric Cauchy–Poisson problem is ill posed in the sense of stability for a certain class of initial conditions, so that the propagating solutions do not depend continuously on the initial conditions. In order to overcome the difficulty of the discontinuity, Landweber–Fridman's regularization, famous and well known in applied mathematics, are introduced and investigated to learn whether it is applicable to the present axisymmetric wave propagation problem. From the numerical experiments, it is shown that stable and accurate solutions are realized by the regularization, so that it can be applicable to the determination of the ill-posed Cauchy–Poisson problem.  相似文献   

辐射沙洲南翼“水道-沙洲”系统与西太阳沙的稳定   总被引：2，自引：0，他引：2  

侯庆志  徐敏  陆培东 《海洋科学》2006,30(6):16-22

通过对江苏岸外辐射沙洲动态和西太阳沙海域动力泥沙环境的分析,论述了西太阳沙演变的宏观背景;由实测固定断面资料、地形资料的对比得出近40年来西太阳沙表面形态和水下地形的变化;结合西太阳沙表面泥沙在波浪和波流共同作用下运动特性的水槽试验研究,分析了西太阳沙冲淤变化的动力机制。从“烂沙洋水道-西太阳沙-西太阳沙南水道”系统的角度研究了西太阳沙演变的控制因素和演变规律。  相似文献   

Remote Sensing Analysis of the Suspended Sediment Transport in Lingdingyang   总被引：1，自引：0，他引：1  

You Yuming Hou Min Senior Engineer  River  Harbour Department  Nanjing Hydraulic Research Institute    Nanjing Assistant Engineer  River  Harbour Department  Nanjing Hydraulic Research Institute    Nanjing 《中国海洋工程》1992,(3)

- The data of landsat TM of multi-temporal for Lingdingyang Estuary, Pearl River in China is firstly used with suspended sediment concentration of field measurement to establish a correlative model equation. After the ratio processing of TM data and atmospheric correction, the images of suspended sediment concentration of different temporals are exported from the image processing systems AREIS II and III. These images express the characteristics of suspended sediment distribution, the mode of sediment transport and the extent of dispersion under the actions of tidal current and wind condition of different seasons.  相似文献   

Distribution of dissolved organic carbon in and near the Prydz Bay, Antarctica     

Qiu Yusheng  Chen Min  Huang Yipu  Liu Guangshan 《海洋学报(英文版)》2003,22(4):547-556

INTRODUCTIONDissolvedorganiccarbon (DOC)makesupthesecondlargestofthebioactivepoolsofcar bonintheocean ,secondtothelargestpoolofdissolvedinorganiccarbon .Theglobaldissolvedorganiccarbonpoolisestimatedtobe 6 85Gt,avaluecomparabletothemassofCO2 intheat mosphere (Hedges,1 992 ) .Thesizeofthereservoir,aswellasitsdynamics ,indicatesthatDOCplaysacentralroleintheoceancarboncycle .AsitrelatestogreenhousegasessuchasCO2 andassociatesclimatecycle ,oceanicDOCbiogeochemicalcycleshavebeenoneoftheh…  相似文献   

Null broadening with snapshot-deficient covariance matrices in passive sonar   总被引：1，自引：0，他引：1  

Song  H. Kuperman  W.A. Hodgkiss  W.S. Gerstoft  P. Jea Soo Kim 《Oceanic Engineering, IEEE Journal of》2003,28(2):250-261

Adaptive-array beamforming achieves high resolution and sidelobe suppression by producing sharp s in the adaptive beampattern. Large-aperture sonar arrays with many elements have small resolution cells; interferers may move through many resolution cells in the time required for accumulating a full-rank sample covariance matrix. This leads to "snapshot-deficient" processing. In this paper, the -broadening technique originally developed for an ideal stationary problem is extended to the snapshot-deficient problem combined with white-noise constraint (WNC) adaptive processing. Null broadening allows the strong interferers to move through resolution cells and increases the number of degrees of freedom, thereby improving the detection of weak stationary signals.  相似文献