Screening of extraction methods for glycoproteins from jellyfish (Rhopilema esculentum) oral-arms by high performance liquid chromatography     

Guoyan Ren  Bafang Li  Xue Zhao  Yongliang Zhuang  Mingyan Yan  Hu Hou  Xiukun Zhang  Li Chen 《中国海洋大学学报(英文版)》2009,8(1):83-88

In order to select an optimum extraction method for the target glycoprotein (TGP) from jellyfish (Rhopilema esculentum) oral-arms, a high performance liquid chromatography (HPLC)-assay for the determination of the TGP was developed. Purified target glycoprotein was taken as a standard glycoprotein. The results showed that the calibration curves for peak area plotted against concentration for TGP were linear (r = 0.9984, y = 4.5895x+47.601) over concentrations ranging from 50 to 400 mgL-1. The mean extraction recovery was 97.84% (CV2.60%). The fractions containing TGP were isolated from jellyfish (R. esculentum) oral-arms by four extraction methods: 1) water extraction (WE), 2) phosphate buffer solution (PBS) extraction (PE), 3) ultrasound-assisted water extraction (UA-WE), 4) ultrasound-assisted PBS extraction (UA-PE). The lyophilized extract was dissolved in Milli-Q water and analyzed directly on a short TSK-GEL G4000PWXL (7.8 mm×300 mm) column. Our results indicated that the UA-PE method was the optimum extraction method selected by HPLC.  相似文献   

山东梁邹矿业集团充分利用尾矿的成功实践          下载免费PDF全文

董丽丽  任昌胜  刘玉清 《山东国土资源》2009,25(4)

山东梁邹矿业集团有限公司,位于山东省邹平县境内。由邹平钼业有限公司、邹平铭兴化工有限公司、邹平铭波电源有限公司、邹平铭扬建材有限公司、邹平碑楼铜业有限公司、邹平铭远贸易进出口有限公司组成,是集铜钼采选、精细化工、充电电池、建筑材料、进出口贸易和职工现代服务等为一体的综合性企业集团。其中,钼业公司、化工公司属省二级安全标准化企业,钼业公司是山东省唯一铜钼采选企业,在2000年国家统计局公布的全国最大100家有色金属采选企业中排名第32位。  相似文献   

Distribution patterns of interstitial polychaetes in sandy beaches of southern Brazil   总被引：1，自引：0，他引：1  

Maikon Di Domenico  Paulo da Cunha Lana  & André R. S. Garraffoni 《Marine Ecology》2009,30(1):47-62

This study describes the distribution patterns of interstitial polychaetes along morphodynamic gradients on six exposed sandy beaches in Santa Catarina and Paraná (South Brazil). Three random transects were sampled at two points on each beach, one at the swash and another at the surf zone, in winter and summer conditions. Six sediment replicates were collected at each sampling point using a corer of 4.6 cm internal diameter that removed 10 cm into the sediment. Abundance and composition of interstitial polychaete were correlated to wave height, slope, grain size, CaCO₃, chlorophyll a , omega indexes, temperature and relative tide range using a canonical correspondence analysis (CCA). A factorial ANOVA showed that taxa richness, mean density and Shannon's diversity were significantly higher at the reflective beaches, but average values differ significantly between transects and these differences change according to the beach zones on both sampling dates. PERMANOVA showed that polychaete associations differ among transects according to the beach zones. The composition of interstitial polychaete associations was significantly correlated to beach morphodynamics and features (P < 0.01). Polychaete associations of reflective beaches were more diverse than in other morphodynamic states. Intermediate beaches may also sustain diverse associations due to temporal variability of the morphodynamic patterns. Beaches presenting extreme dissipative morphodynamics and compacted sediments appear to be unfavourable for the occurrence of interstitial polychaetes.  相似文献   

Effect of food quality on energy uptake     

Jeffrey S. Ren   《Journal of Sea Research》2009,62(2-3):72

The dynamic energy budget (DEB) theory assumes that the feeding of an organism in relation to food density follows the type-II functional response when food consists of purely digestible organic matter. However when faced with a food source high in inorganic content, an organism needs to adapt physiologically to maximise organic extraction. Indeed filter-feeding marine bivalves inhabiting turbid coastal waters cope with large fluctuations in food quality by selectively ingesting and pseudofaeces production. Data from feeding experiments with the New Zealand greenshell mussel Perna canaliculus have demonstrated that inorganic particles contribute some uncertainty to the estimate of the half-saturation coefficient. Incorporating inorganic matter into the functional response would improve the prediction of energy uptake.  相似文献   

Cr^3＋在改善三元复合驱中的作用实验研究     

任艳滨  卢祥国  赵兰兰  张运来 《海洋地质译丛》2008,(4):50-54

在三元复合驱矿场应用实践中,由于三元复合体系自身流度控制能力的局限性,必须通过聚合物凝胶前置段塞来改善吸液剖面,这就涉及到聚合物凝胶与三元复合体系的配伍性。通过向三元复合体系中加入有机铬,考察Cr^3＋对三元复合体系黏度、界面张力和阻力系数等性能影响,进而对Cr^3＋聚合物凝胶与三元复合体系配伍性以及Cr^3＋在改善三元复合体系性能方面的作用做出评价。结果表明,Cr^3＋的加入对三元复合体系黏度和界面张力影响不大,但阻力系数和残余阻力系数却明显增加。当采用Cr^3＋聚合物凝胶作为前置段塞或采用添加Cr^3＋的三元复合体系进行复合驱油实验时,其采收率增加幅度都要高于普通三元复合驱。这不仅显示出Cr^3＋聚合物凝胶与三元复合体系间良好的配伍性,而且为改善三元复合驱增油效果提供了新的途径。  相似文献   

Reply to comments of G. Abril on “Underwater measurements of carbon dioxide evolution in marine plant communities: A new method” by J. Silva and R. Santos [Estuarine,Coastal and Shelf Science 78(2008) 827–830]     

João Silva  Rui Santos 《Estuarine, Coastal and Shelf Science》2009

  相似文献   

2004 年夏初、秋末长江口外海区冲淡水及羽状锋的盐度特征   总被引：1，自引：1，他引：0  

郑琰明  康建成  钟巍  周尚哲  王芳  徐韧  孙瑞文  吴涛 《海洋与湖沼》2009,40(3):275-282

用国家海洋局东海环境监测中心2004年夏初（6月）和秋末（11月）对长江口外海区现场观测资料,用Matlab计算机编程语言对观测资料进行质量控制、插值网格化,绘制研究海区盐度立体分布图,显示夏初、秋末两季的盐度分布特征。将2004年夏、秋两季长江口外海区冲淡水和羽状锋的分布与以往观测研究结果对比,可得：（1）长江口外海区冲淡水的盐度值主要在5—31之间,冲淡水前缘盐度锋面的盐度值为18—28;盐度值20—28的盐度锋面基本体现该海区羽状锋的特征及变化。（2）夏初长江口外海区冲淡水范围明显大于秋末,夏初该海区以冲淡水为主,秋末冲淡水的范围收缩,紧贴沿岸一带。相应地,夏初该海区羽状锋呈倾斜锋面,占据较大的海区;秋末则为垂直锋面,介于122．50&#176;E-122．70&#176;E海区。  相似文献   

Distribution patterns of chlorophyll <Emphasis Type="Italic">a</Emphasis> in spring and autumn in association with hydrological features in the southern Yellow Sea and northern East China Sea     

张芳  李超伦  孙松  吴玉霖  任敬萍 《中国海洋湖沼学报》2009,27(4):784-792

Two field studies were conducted to measure pigments in the Southern Yellow Sea (SYS) and the northern East China Sea (NECS) in April (spring) and September (autumn) to evaluate the distribution pattern of phytoplankton stock (Chl a concentration) and the impact of hydrological features such as water mass, mixing and tidal front on these patterns. The results indicated that the Chl a concentration was 2.43±2.64 (Mean ± SD) mg m^?3 in April (range, 0.35 to 17.02 mg m^?3) and 1.75±3.10 mg m^?3 in September (from 0.07 to 36.54 mg m^?3) in 2003. Additionally, four areas with higher Chl a concentrations were observed in the surface water in April, while two were observed in September, and these areas were located within or near the point at which different water masses converged (temperature front area). The distribution pattern of Chl a was generally consistent between onshore and offshore stations at different depths in April and September. Specifically, higher Chl a concentrations were observed along the coastal line in September, which consisted of a mixing area and a tidal front area, although the distributional pattern of Chl a concentrations varied along transects in April. The maximum Chl a concentration at each station was observed in the surface and subsurface layer (0–10 m) for onshore stations and the thermocline layer (10–30 m) for offshore stations in September, while the greatest concentrations were generally observed in surface and subsurface water (0–10 m) in April. The formation of the Chl a distributional pattern in the SYS and NECS and its relationship with possible influencing factors is also discussed. Although physical forces had a close relationship with Chl a distribution, more data are required to clearly and comprehensively elucidate the spatial pattern dynamics of Chl a in the SYS and NECS.  相似文献   

利用双阳台水管观测资料检测地球自由振荡   总被引：2，自引：2，他引：0  

邓娜  刘冰冰  杨耀文  蒋翠荣  范金梅  任佳 《大地测量与地球动力学》2009,29(Z1):41-44

基于双阳台水管倾斜仪的数字化观测资料,利用功率谱密度估计方法,在没有对资料进行去固体潮处理的情况下,准确获得了2004年12月26日苏门答腊大地震激发的0S3～0S31基频球型自由振荡。并与PREM模型的理论自由振荡周期进行了对比,发现实测振荡周期与PREM预测的振荡周期相吻合,除0S3、0S4振型的观测周期和PREM模型理论周期的相对误差大于0.3%外,其他振型的观测周期和PREM模型理论周期的相对误差大都集中在0.1%左右。同时还检测到了5个谐频球型振荡和8个环型振荡。  相似文献   

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兰小机  任金铜 《大地测量与地球动力学》2009,29(6):139-143

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