A comparison of different Gracilariopsis lemaneiformis (Rhodophyta) parts in biochemical characteristics,protoplast formation and regeneration     

Zhongxia Wang  Zhenghong Sui  Yiyi Hu  Si Zhang  Yulong Pan  Hongri Ju 《中国海洋大学学报(英文版)》2014,13(4):671-676

Gracilariopsis lemaneiformis is a commercially exploited alga. Its filaceous thallus can be divided into three parts, holdfast, middle segment and tip. The growth and branch forming trend and agar content of these three parts were analyzed, respectively, in this study. The results showed that the tip had the highest growth rate and branched most, although it was the last part with branch forming ability. The holdfast formed branches earliest but slowly. Holdfast had the highest agar content. We also assessed the difference in protoplast formation and regeneration among three parts. The middle segment displayed the shortest enzymolysis time and the highest protoplast yield; whereas the tip had the strongest vitality of protoplasts formation. Juvenile plants were only obtained from the protoplasts generated from the tip. These results suggested that the differentiation and function of G. lemaneiformis was different.  相似文献   

Characterizing landscape spatial heterogeneity in multisensor images with variogram models     

Bingwen Qiu  Canying Zeng  Chongcheng Cheng  Zhenghong Tang  Jianyang Gao  Yinpo Sui 《中国地理科学(英文版)》2014,24(3):317-327

Most evaluation of the consistency of multisensor images have focused on Normalized Difference Vegetation Index （NDVI） products for natural landscapes, often neglecting less vegetated urban landscapes. This gap has been filled through quantifying and evaluating spatial heterogeneity of urban and natural landscapes from QuickBird, Satellite pour l＇observation de la Terre （SPOT）, Ad- vanced Spacebome Thermal Emission and Reflection Radiometer （ASTER） and Landsat Thematic Mapper （TM） images with variogram analysis. Instead of a logarithmic relationship with pixel size observed in the corresponding aggregated images, the spatial variability decayed and the spatial structures decomposed more slowly and complexly with spatial resolution for real multisensor im- ages. As the spatial resolution increased, the proportion of spatial variability of the smaller spatial structure decreased quickly and only a larger spatial structure was observed at very coarse scales. Compared with visible band, greater spatial variability was observed in near infrared band for both densely and less densely vegetated landscapes. The influence of image size on spatial heterogeneity was highly dependent on whether the empirical sernivariogram reached its sill within the original image size. When the empirical semivariogram did not reach its sill at the original observation scale, spatial variability and mean characteristic length scale would increase with image size; otherwise they might decrease. This study could provide new insights into the knowledge of spatial heterogeneity in real multisen- sor images with consideration of their nominal spatial resolution, image size and spectral bands.  相似文献   

Discrete element modelling of material non‐coaxiality in simple shear flows     

Jun Ai  Paul A. Langston  Hai‐Sui Yu 《国际地质力学数值与分析法杂志》2014,38(6):615-635

We investigate the quasi‐static simple shear flow of a two‐dimensional assembly of cohesionless particles using discrete element method (DEM) simulations. We focus on the unsteady flow regime where the solid would experience significant evolution of stresses, mobilised shear strength and dilation. We construct the DEM model using a discretised‐wall confined granular cell where the apparent boundary is allowed to dilate or contract synchronously with the confined solid. A rather uniform simple shear field is achieved across the whole assembly, which benefits rheological studies in generalising constitutive laws for continuum methods. We examine two aspects of the simple shear behaviour: macroscopic stress and strain rate evolution, particularly the non‐coaxiality between the principal directions of the two; and micromechanics such as evolution of fabric. For an initially anisotropic specimen sheared under constant normal pressure, the direction of principal stress rotates towards that of the principal strain rate, gradually reducing the degree of non‐coaxiality from about 45° to fluctuating around 0°. The rate in approaching coaxiality is slower in samples with larger initial porosity, stress ratio and mean stress. Generally, a faster rate in approaching coaxiality in simple shear is observed in a more dilatant sample, which often shows a larger degree of mobilised fabric anisotropy, suggesting the possible important role of instantaneous internal friction angle. The evolution of principal fabric direction resembles that of the principal stress direction. © 2013 The Authors. International Journal for Numerical and Analytical Methods in Geomechanics published by John Wiley & Sons, Ltd.  相似文献   

A virtual experiment technique on the elementary behaviour of granular materials with discrete element method     

Xia Li  Hai‐Sui Yu  Xiang‐Song Li 《国际地质力学数值与分析法杂志》2013,37(1):75-96

Multi‐scale investigations aided by the discrete element method (DEM) play a vital role for current state‐of‐the‐art research on the elementary behaviour of granular materials. Similar to laboratory tests, there are three important aspects to be considered carefully, which are the proper stress/strain definition and measurement, the application of target loading paths and the designed experiment setup, to be addressed in the present paper. Considering the volume sensitive characteristics of granular materials, in the proposed technique, the deformation of the tested specimen is controlled and measured by deformation gradient tensor involving both the undeformed configuration and the current configuration. Definitions of Biot strain and Cauchy stress are adopted. The expressions of them in terms of contact forces and particle displacements, respectively, are derived. The boundary of the tested specimen consists of rigid massless planar units. It is suggested that the representative element uses a convex polyhedral (polygonal) shape to minimize possible boundary arching effects. General loading paths are described by directly specifying the changes in the stress/strain invariants or directions. Loading can be applied in the strain‐controlled mode by specifying the translations and rotations of the boundary units, or in the stress‐controlled mode by using a servo‐control mechanism, or in the combination of the two methods to realize mixed boundary conditions. Taking the simulation results as the natural consequences originated from a complex system, virtual experiments provide particle‐scale information database to conduct multi‐scale investigations for better understanding in granular material behaviours and possible development of the constitutive theories provided the qualitative similarity between the simulation results from virtual experiments and observations on real material behaviour. Copyright © 2011 John Wiley & Sons, Ltd.  相似文献   

Cloning and Characterization of Glyceraldehyde-3-phosphate Dehydrogenase Encoding Gene in Gracilaria/Gracilariopsis lemaneiformis     

REN Xueying SUI Zhenghong ZHANG Xuecheng 《中国海洋大学学报(英文版)》2006,5(2):146-150

Glyceraldehyde-3-phosphate dehydrogenase （GAPDH） plays important roles in various cellular processes. A cytosolic GAPDH encoding gene （gpd） of Gracilaria/Gracilariopsis lemaneiformis was cloned and characterized. Deduced amino acid sequence of the enzyme of G. lemaneiformis had high homology with those of seven red algae. The 5＇-untranslated regions of the GAPDHs encoding genes of these red algae varied greatly. GAPDHs of these red algae shared the highly conserved glyceraldehyde 3-phosphate dehydrogenase active site ASCTTNCL. However, such active site of Cyanidium caldarium was different from those of the other six algae at the last two residues （CL to LF）, thus the spatial structure of its GAPDH active center may be different from those of the other six. Phylogenetic analysis indicated that GAPDH of G. lemaneiformis might have undergone an evolution similar to those of Porphyra yezoensis, Chondrus crispus, and Gracilaria verrucosa. C. caldarium had a closer evolutionary relationship with Cyanidioschyzon merolae than with Cyanidium sp. Virtual Northern blot analysis revealed that gpd of G. lemaneiformis expressed constitutively, which suggested that it might be house-keeping and could be adapted as an inner control in gene expression analysis of G. lemaneiformis.  相似文献   

不等视距水准测量应用及精度分析     

何思源  范东明  谷延超  郑蓉 《测绘科学》2018,(2):121-124

在水准测量作业过程中,为减小视距差引起的系统误差,相关规范对水准测量前后视距差及视距累积差进行了严格的控制,而在实际的工作中对前后视距差的控制又极大地限制了水准测量的应用领域以及工作效率。针对此问题,该文提出将i角当作系统参数进行平差的方法,平差结束即可得到i角值以及经过i角误差改化后的高程平差值。对比分析了附加系统参数平差法与条件函数法,实例表明附加系统参数平差法具有更好的高效性和稳健性。  相似文献   

黄河三角洲浅层地下水盐分来源及咸化过程研究     

侯国华  高茂生  叶思源  赵广明 《地学前缘》2022,29(3):145-154

黄河三角洲地下水咸化已成为区域最突出的生态环境问题之一。识别地下水补给及盐分来源是有效控制和改善地下水咸化问题的关键。本研究采集了研究区浅层地下水、地表水和海水等不同类型水样,利用离子比、Piper三线图、吉布斯图等方法对八大离子浓度、δD和δ¹⁸O 组成、Br和Sr 浓度等进行地下水补给研究与盐分来源辨析。结果表明:(1)黄河三角洲浅层地下水以总溶解性固体(TDS)为338 g/L的咸水为主,地下水水化学类型较为单一,主要为Cl-Na型。(2)三角洲区域地下水以大气降水补给为主,并且在补给过程中经历了不同程度的蒸发作用的影响,黄河现行流路区域地下水主要来源于河水侧渗补给,但浅层地下水含水层水平渗透性较差限制了黄河侧渗补给范围。(3)海洋是黄河三角洲浅层地下水盐分的主要来源,黄河现行流路区域及近岸地下水盐分来源于海水混合,三角洲北部刁口河等古河道区域地下水盐分主要来源于海相蒸发盐淋滤溶解。  相似文献   

Fabric evolution and liquefaction resistance in multiple-liquefaction process: a micromechanical study using DEM-clump     

Yang  Siyuan  Huang  Duruo 《Acta Geotechnica》2022,17(12):5655-5674

Acta Geotechnica - Recent earthquake case histories in Japan and New Zealand revealed that soil sites can experience liquefaction multiple times under a sequence of earthquake shaking. Field...  相似文献   

基于SEM的湖南省暴雨气象防灾减灾服务公众满意度评估          下载免费PDF全文

邓玲  张思远  李学敏 《气象与减灾研究》2019,42(4):306-311

以湖南省暴雨气象防灾减灾服务为研究视角,首先对湖南省气象局暴雨气象防灾减灾服务效益评估研究组抽样调查得到的7 478份问卷数据进行无效问卷剔除,在此基础上,从暴雨预报的准确性、预警发布的及时性、信息发布的频次、获取信息的便捷性、内容的通俗性及防御建议的有效性六个方面,分析公众暴雨气象防灾减灾服务满意度。采用结构方程模型,评估各个因素对暴雨气象防灾减灾服务公众满意度的影响。最后利用所得到的各个路径系数建立暴雨气象防灾减灾服务公众满意度评估模型,代入调查问卷采集到的数据后得到湖南省暴雨气象防灾减灾服务公众满意度为88.02%。  相似文献   

强台风海葵登陆前后强度变化的观测分析   总被引：2，自引：2，他引：0  

钱燕珍  高拴柱  黄思源  杜坤  郭建民  郭宇光 《气象》2013,39(10):1265-1274

1211号台风海葵强度强,在近海及登陆前后中、日、美三个国家的业务定强差别较大。云图分析“海葵”强度强,近海后云顶亮温长时间在-70℃以下。雷达资料表明“海葵”登陆前后,结构对称,眼区清晰,范围增大。登陆点附近沿海测站出现了长时间的强大风,过程13级及以上阵风共持续了26 h,东矶、大陈、石浦最大风速的极大值分别是：46.9、39.4和36.8 m·s-1。风场垂直分布差别500 m以内普遍在7~8 m·s-1。这些陆基观测资料表明“海葵”可能达到了强台风标准,中央气象台定强是合理的。近海或登陆台风强度的确定在使用Dvorak技术的基础上,还应更多地参考陆基探测资料。  相似文献