用于海洋沉积物中厌氧氨氧化细菌分子生态学研究的引物比较     

付璐璐  李思琦  甄毓  米铁柱 《中国海洋大学学报(自然科学版)》2019,(9):77-88

为比较厌氧氨氧化细菌16SrRNA基因的2对特异性引物(Amx368F/Amx820R、Brod541F/Amx820R)在其分子生态学研究方面的优点与不足,本研究采用高通量测序和qPCR技术对长江口及其邻近海域沉积物中厌氧氨氧化细菌细菌的群落组成、多样性和丰度进行比较分析,结果表明,引物Amx368F/Amx820R特异性较高,能够较为完整地反映沉积物中厌氧氨氧化细菌的多样性信息,且其覆盖的厌氧氨氧化细菌丰度与功能基因hzo丰度正相关(P<0.01);而引物Brod541F/Amx820R特异性较低,覆盖部分厌氧氨氧化细菌及其他多个门的细菌,其量化的厌氧氨氧化细菌与功能基因hzo丰度无明显相关关系(P>0.05)。因此,引物Amx368F/Amx820R能够更加真实地反映厌氧氨氧化细菌在海洋沉积物中的群落特征,在其分子生态学研究中更具优势。  相似文献   

Succession of the Nanoplankton Community during a Brown Tide in a Scallop Culture Area in China     

Qiao  Ling  Yu  Jie  Li  Ying  Guo  Haobing  Zhen  Yu  Zhang  Lingling  Mi  Tiezhu  Zhang  Fuchong  Bao  Zhenmin 《Ocean Science Journal》2019,54(3):375-392

Ocean Science Journal - Brown tides caused by Aureococcus anophagefferens have occurred frequently in the coastal waters of Qinhuangdao since 2009. The Changli coast is the area most strongly...  相似文献   

柔肝通络汤治疗脑梗死急性期40例临床观察          下载免费PDF全文

及晓梦  刘雨  周慎 《应用海洋学学报》2019,35(2):6-8

目的：观察柔肝通络汤治疗脑梗死急性期（阴虚络瘀证）的临床疗效。方法：将脑梗死急性期（阴虚络瘀证）患者80例,随机分为2组,每组各40例。治疗组采用柔肝通络汤治疗,对照组采用西医常规治疗。结果：综合疗效总有效率治疗组为87.5%,对照组为72.5%,组间比较,差异有统计学意义（P＜0.05）;中医证候疗效总有效率治疗组为90.0%,对照组为75.0%,组间比较,差异有统计学意义（P＜0.05）;2组血液流变学指标、NIHSS评分治疗前后组内比较及治疗后组间比较,差异均有统计学意义（P＜0.01）。结论：柔肝通络汤治疗脑梗死急性期（阴虚络瘀证）有较好疗效,优于西医常规治疗。  相似文献   

结合MST划分和RHMRF-FCM算法的高分辨率遥感图像分割     

林文杰  李玉  赵泉华 《测绘学报》2019,48(1):64-74

针对基于像素的HMRF-FCM算法抗噪性差以及对地物复杂边界分割精度低的问题,提出一种结合形状信息的静态MST区域划分和RHMRF-FCM算法的高分辨率遥感图像分割方法。该方法定义一种静态MST同质区域划分准则,借助MST能较好表达边界和形状信息、能较好抑制几何噪声的特点,解决地物复杂边界的表达和降低分割结果中几何噪声问题。首先,利用MST静态划分将图像域划分成若干个均质区域,假设每个均质区域内光谱测度服从独立同一的多元高斯分布。然后,在此基础上构建了区域隐马尔可夫随机场模型,以及建立基于信息熵和KL信息正则化项的模糊聚类目标函数。最后,采用偏微分方法对分割模型参数进行求解,从而得到全局最优分割结果。为验证本文方法,对WorldView-3高分遥感图像进行分割试验。定性、定量分析了尺度参数、光谱相似性参数和区域紧致度参数对最优分割结果的影响,并对比分析本文算法和eCognition软件中的多分辨率分割算法、分水岭算法。  相似文献   

Optical Identification of Four Hard X-ray Sources from the INTEGRAL Sky Surveys     

Karasev  D. I.  Sazonov  S. Yu.  Tkachenko  A. Yu.  Khorunzhev  G. A.  Krivonos  R. A.  Medvedev  P. S.  Zaznobin  I. A.  Mereminskiy  I. A.  Burenin  R. A.  Pavlinsky  M. N.  Eselevich  M. V. 《Astronomy Letters》2019,45(12):836-846

Astronomy Letters - We continue the study begun in Karasev et al. (2018) and present the results of our optical identifications of four hard X-ray sources from the INTEGRAL sky surveys. Having...  相似文献   

Evolution and Pulsations of Population I Post-AGB Stars     

Fadeyev  Yu. A. 《Astronomy Letters》2019,45(8):521-527

Astronomy Letters - Evolutionary calculations of population I stars with initial masses M0 = 1 M⊙, 1.5 M⊙ and 2 M⊙ were carried out up to the stage of the proto-planetary nebula....  相似文献   

一种高光谱遥感图像快速谱聚类算法     

张亚平  张宇  杨楠  罗晓  罗谦 《测绘通报》2019,(12):60-64

为获得分类效果更优良的遥感图像分类方式并解决高光谱遥感图像分类运算速度缓慢的问题,集成Lanczos算法与谱聚类算法,探讨了高光谱遥感图像谱聚类算法应用于遥感图像分类的可行性,提出了一种面向高光谱遥感图像的快速谱聚类算法;通过对比美国圣地亚哥机场高光谱遥感图像K-均值算法与谱聚类算法的分类结果,发现面向高光谱遥感图像的谱聚类算法易于识别线性地物,且分类的速度能得到较大提升。  相似文献   

An improved building detection approach using L-band POLSAR two-dimensional time-frequency decomposition over oriented built-up areas     

Lei Deng  Ying He  Zhi-hui Mao  Jie Yu 《地理信息系统科学与遥感》2019,56(1):1-21

The objective of this study is to efficiently extract detailed information about various man-made targets in oriented built-up areas using polarimetric synthetic aperture radar (POLSAR) images. This paper develops an improved approach for building detection by utilizing Two-Dimensional Time-Frequency (2-D TF) decomposition. This method performs outstandingly in distinguishing between man-made and natural targets based on the isotropic behaviors, frequency-sensitive responses, and scattering mechanisms of objects. The proposed method can preserve the spatial resolution and exploit the advantages of TF decomposition; specifically, the exact outlines of buildings can be effectively located, and more types of features (e.g., flat roofs, roads, and walls that are oblique to the radar illumination) can be distinguished from forests in complex built-up areas by 2-D TF decomposition. The coarser-resolution subaperture images that are produced in the azimuth direction, which correspond to different looking angles, are beneficial for detecting man-made structures with main scattering centers oriented at oblique angles with respect to the radar illumination. In the range direction, the obtained subaperture images, which correspond to various observation frequencies, can be helpful in distinguishing flat roofs and roads from forests. This method was successfully implemented to analyze both NASA/JPL L-band AIRSAR and L-band EMISAR data sets. The building detection results of the proposed method exhibit a significant improvement over those of other methods and reach an overall accuracy over 80%, with approximately 20% higher than the accuracies of K-means clustering and the entropy/alpha-Wishart classifier and approximately 10% higher than the accuracy of the support vector machine method. Moreover, building details can be precisely detected, obliquely oriented buildings can be identified, and the distinction between buildings and forests is significantly improved, as both visually and statistically indicated. This method is highly adaptable and has substantial application value.  相似文献   

自动化监测系统在深基坑监测中的可靠性分析     

王宇  王鹏  李铭  于峰 《测绘与空间地理信息》2019,42(3):222-224

深基坑监测对于城市工程的安全建设具有重要的指导意义。为了探究自动化监测系统在城市深基坑监测中的可行性,根据全站仪的测量原理利用Matlab进行三维仿真,从理论上证明了自动化监测系统的平面精度能够达到规范要求。采用尺度变形分析及水平位移对比分析,论证了在城市深基坑监测中利用自动化监测系统进行水平位移监测的可靠性。  相似文献   

A method for aligning the plastic scintillator detector on DAMPE     

Peng-Xiong Ma  Yong-Jie Zhang  Ya-Peng Zhang  Yao Li  Jing-Jing Zang  Xiang Li  Tie-Kuang Dong  Yi-Zhong Fan  Shi-Jun Lei  Jian Wu  Yu-Hong Yu  Qiang Yuan  Chuan Yue  Zhi-Yu Sun 《天文和天体物理学研究(英文版)》2019,(6):63-70

The Plastic Scintillator Detector(PSD) onboard the DArk Matter Particle Explorer(DAMPE)is designed to measure cosmic ray charge(Z) and to act as a veto detector for gamma ray identification.To fully exploit the charge identification potential of PSD and to enhance its capability to identify gamma ray events, we develop an alignment method for the PSD. The path length of a given track in the volume of a PSD bar is derived taking into account the shift and rotation alignment corrections. By examining energy spectra of corner-passing events and fully contained events, position shifts and rotations of all PSD bars are obtained, and are found to be on average about 1 mm and 0.0015 radian respectively. To validate the alignment method, we introduce artificial shifts and rotations of PSD bars into the detector simulation.These shift and rotation parameters can be recovered successfully by the alignment procedure. As a result of the PSD alignment procedure, the charge resolution of the PSD is improved from 4% to 8%, depending on the nuclei.  相似文献