Induction of vitellogenin in vivo and in vitro in the model teleost medaka (Oryzias latipes): comparison of gene expression and protein levels   总被引：5，自引：0，他引：5  

Scholz S  Kordes C  Hamann J  Gutzeit HO 《Marine environmental research》2004,57(3):235-244

Quantification of the egg yolk precursor vitellogenin (VTG) in fish has become a standard technique to detect estrogenic effects of known chemicals and environmental samples. In the present study, we have analysed VTG induction by estradiol, ethynylestradiol and genistein exposure in the model teleost medaka (Oryzias latipes) and demonstrate that the medaka is a suitable model system to analyse estrogenic effects. By comparing VTG gene expression and protein levels we show that in principal both techniques can be used to study VTG induction in vivo (juvenile and adult males) and in vitro (primary cultures of male liver cells). If a short term in vivo or in vitro exposure is performed, detection of mRNA might be sufficient. For long term studies with the need to detect weak estrogenic chemicals and a precise quantification, immuno-chemical detection may be favoured.  相似文献   

Measuring lysosomal stability as an effective tool for marine coastal environmental monitoring     

Castro M  Santos MM  Monteiro NM  Vieira N 《Marine environmental research》2004,58(2-5):741-745

The use of lysosomal stability in the mussel, Mytilus galloprovincialis, as a potential biomarker of environmental contamination has been evaluated along the Portuguese coast. To this end, the neutral red retention (NRR) time was measured in mussel haemocytes gathered from nine different locations reflecting different degrees of anthropogenic contamination. Mussels collected in the vicinity of industrial and urban areas showed the lowest lysosomal stability. Additionally, no significant seasonal variability (winter-spring/summer) for NRR time was observed. In order to further support the usefulness of this method as an integrated tool for monitoring marine coastal environments, we compared the levels of xenobiotics in mussel tissues with the obtained NRR values. The results highlighted a consistent pattern, with the lowest lysosomal stability intimately correlated with the higher contaminant concentrations. In summary, this integrated approach further demonstrated that the NRR assay can provide useful and objective indications of the real health status of organisms subjected to different stress agents, being a valid option for environmental monitoring.  相似文献   

前陆盆地向稳定状态的演化：来自台湾西部前陆盆地的证据   总被引：1，自引：0，他引：1  

Covey  M. 项光 《海洋地质译丛》1989,(1):36-43

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粉红色红拟抱球虫在冲绳海槽区的发现及地层意义   总被引：3，自引：1，他引：3  

苍树溪  N.J.Shackleton  秦蕴珊  阎军 《海洋地质与第四纪地质》1988,(1)

近年来,我们在冲绳海槽区进行大量工作.本文就Z14-6孔(27°07′N127°27′E)首次发现粉红色红拟抱球虫,及稳定同位素测定,进行对比研究,证明了Thompson等1979年所提出的粉红色拟抱球虫在印度-太平洋海域灭绝时代在距今120000年左右的观点.  相似文献   

碳同位素质谱法探讨现代海洋有机沉积物来源     

朱桂海  Jame M.Brooks 《海洋学研究》1986,(2)

本文用NUCLIDE RMS 6-60质谱仪测定了长江口及邻近海区(北纬30°06′至31°15′,东经122°30′至124°30′)表层沉积物中有机碳稳定同位素组成(δ~(13)C为-23.6×10~(-3)—-21.3×10~(-3))、有机碳含量(0.4—0.1％),溶剂可提取有机物浓度及有机C/N比值。结果表明,从有机地球化学角度解释并证实了其他地学家在该区域的研完成果,即东海陆架泥质沉积物主要由陆源悬浮物和海洋浮游生物体混合组成,长江入海物质随着远离长江口而递减,大部分陆源悬浮物向东南运移,并沉积在东经123°以西的长江口区。  相似文献   

Upper temperature tolerances of exotic brackish-water mussel, Mytilopsis leucophaeata (Conrad): an experimental study   总被引：1，自引：0，他引：1  

Rajagopal S  Van der Gaag M  Van der Velde G  Jenner HA 《Marine environmental research》2005,60(4):512-530

The dark false mussel, Mytilopsis leucophaeata is an important mussel colonising the brackish-water systems of temperate and subtropical regions. Of late it has earned notoriety as a biofouling species in industrial cooling water systems. However, there are no published data on the temperature tolerance of this species. This paper presents data on the upper temperature tolerance of this mussel from the view point of biofouling control using thermal methods. In addition to mortality, response of physiological activities such as oxygen consumption, filtration rate, foot activity and byssus thread production were also studied at temperatures varying from 5 to 35 degrees C. Experiments were also carried out to understand the effect of mussel size, breeding condition, nutritional status and acclimation conditions (temperature and salinity) on the mortality pattern. The physiological activities were significantly reduced at temperatures beyond 27.5 degrees C and ceased at 35 degrees C. In 20 mm size group mussels exposed to 37 degrees C, 50% mortality was observed after 85 min and 100% mortality after 113 min. The effect of mussel size on mortality at different temperatures was significant, with the larger size group mussels showing greater resistance. M. leucophaeata collected during the non-breeding season (December-April) were more tolerant to temperature than those collected during the breeding season (June-October). Nutritional status of the mussel had no significant influence on the thermal tolerance of the mussel: fed and starved (non-fed) mussels succumbed to temperature at comparable rates. The effect of acclimation temperature and acclimation salinity on M. leucophaeata mortality at different temperatures was significant. Survival time increased with increasing acclimation temperature and decreased with increasing salinity. In comparison with other co-occurring species such as Mytilus edulis and Dreissena polymorpha, M. leucophaeata appears to be more tolerant to high temperature stress.  相似文献   

美国东南部大陆边缘的天然气水合物利用宽角地震资料进行全波形约束和走时反演     

J.Korenaga  张光学 《海洋地质》2002,(1):21-44

利用BSR已完成美国东南边缘大约50000km2的地区编图，并已证实可能与大量天然气水合物有关。1992年6月，同时获得该地区的单道地震和宽角海底地震资料，主要集中在布莱克洋脊和卡罗来纳海隆，能清楚地观察到来自BSR的宽角反射偏移距（达6km）。为了了解区域性的变化和生成卡罗纳海隆高背景速度的二维平均速度模型，用宽角和垂直入射资料进行走时反演，然后做全波形反演业确定BSR的地震成因。最合适的模型显示了两处的BSR之下具相似的低速（1.4km/s），暗示圈闭了低饱和度（10％）的游离气，反演结果也显示有一薄层高速楱状体，最大速度为2.3km/s，刚好位于布莱克洋脊的BSR上方；计算了沉积物反射率，观察到卡罗来纳海隆具有较高的反射率，BSR之下的反射率增加几乎与两个站位的含气带对应。根据速度模型估算了水合物的浓度。另一方面，占沉积物总体积3%的水合物之平均浓度暗示布莱克洋脊地区水合物稳定带厚度少一半。卡罗来纳海隆水合物平均 浓度最大为20%，卡罗来纳海隆7%。这两处水合物浓度高，无法仅用原地生物活动来解释，可能与次生的聚集机制不关，因稳定域迁移引起水合物循环，可能影响两地的水合物凝聚。另外，流体向上排出的增加，也可以使 布莱克洋脊生存下去。  相似文献   

An Experi mental Study on Wave-Induced Dynamic Stresses in Seabed     

S.C.Chang  L.K.Chien  J .G.Lin  Y.F.Chiu 《中国海洋工程》2006,20(1)

1 .IntroductionEvery year ,Taiwanis subjectedtotwo or three typhoonintrusions in average which always causedisasters . Most of the disasters which occur around the coastal zone are due to seabed instability andcoastal structure destruction caused bytyphoo…  相似文献   

海洋潜在的能源一天然气水合物     

HarshK．Gupta张明  姚伯初 《海洋地质》2004,(1):48-53

天然气水合物广泛分布在海洋和极地沉积物中。在这种沉积物中具备足够低的温度和足够高的压力使得甲烷气结晶变成水合物。由于水合物的广泛分布和其潜在的能源潜力—据估计其约为地球上所有化石燃料能源的两倍．引起了全世界科学界的关注。然而。至今为止对水合物的性质和分布还了解不够，还没有合适的技术能对水合物进行定量的评价。根据地形、海底温度、沉积条件以及有机碳等资料指出在印度近海具备天然气水合物形成的条件。因此有必要在印度大陆边缘系统开展地质、地球化学和地球物理分析，以识别和估算天然气水合物的储量，评价其资源潜力。类似“气烟囱”或气体溢出持征、地震剖面上的BSR、氯化物或硫化物异常都是识别水合物的有用标志。层析成像、AVO、波形反演是定量分析水合物和游离气含量的重要手段。此外，在地震方法无法识别的地方，电阻率异常也有助干天然气水合物的识别。本文给出一些划出水合物赋存区边界和定量分析水合物及其下伏游离气含量的重要研究成果和技术。  相似文献   

海相碳酸盐岩层序内的石油生成和运移     

Fergu.  J 潘秋霞 《海洋地质译丛》1989,(3):54-61

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