Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis     

Jonsson H  Sandnes KV  Schiedek D  Schneider R  Grøsvik BE  Goksøyr A 《Marine environmental research》2004,58(2-5):655-658

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of approximately 49 kDa size, and weak signals for proteins of approximately 41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of approximately 56 and 59 kDa size, and also a weak immunoreaction with a protein of approximately 43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14-40 ng/g DW in controls to 373-638 ng/g DW in exposed mussels.  相似文献   

烟台海胆体内的纤毛虫和太平洋海胆体内纤毛虫的地理分布     

A.斯特列科夫 《海洋与湖沼》1959,2(1):61-67

1957年夏,中苏海洋动物综合考察队在黄海沿岸烟台地区工作时,参加考察队的阿·阿·斯特列科夫教授采集了海胆肠内的纤毛虫,并以此作为这篇文章的材料。在本文内将提出所见到的纤毛虫名称,并分析它们在太平洋地理分布的情况。  相似文献   

海洋化学的国外发展近况     

纪明侯  李法西 《海洋科学》1979,3(S1):24-27

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Macrofauna impact on Ulva rigida C. Ag. production and relationship with environmental variables in the lagoon of Venice   总被引：2，自引：0，他引：2  

Balducci C  Sfriso A  Pavoni B 《Marine environmental research》2001,52(1):27-49

The grazing pressure of the macrofaunal invertebrates associated with the biomass of Ulva rigida in the Venice lagoon, their species composition and relationship with environmental variables have been studied. Fifteen sampling campaigns were carried out during different seasons both in the central basin of the lagoon, dominated by macroalgae (especially U. rigida C. Ag.) and in the southern basin, prevalently populated by seagrasses (especially Zostera marina L.). Replicate experiments were conducted in the field by exposing Ulva fronds in net cages of 10 and 1 mm (control) mesh-sizes to allow or prevent grazer entrance. The grazing pressure was determined as Ulva growth rate difference in the cages. In the absence of invertebrate herbivores, Ulva exhibited per cent relative growth rates (%RGRs) ranging from 1.5 to 9.5% day(-1), whereas in their presence the %RGRs were significantly lower (from -2.5 to 3.4% day(-1)) and frequently negative, especially in the station dominated by macroalgae. In this area, peak grazing rates and macrofauna biomasses of up to 8.6% day(-1) and 1,480 g m(-2) fwt (84.4 ash-free dry weight), respectively, were found. On the whole, during in field experiments in the Ulva-dominated station, herbivores removed an amount of biomass whose percentage ranged from 59 to 165% (mean: 103%) of the biomass yield (grazers excluded) found in the cages. These results suggest the possibility that grazers could act as an important factor affecting Ulva production in the Venice lagoon. Macrofauna populations were analysed by means of multivaliate techniques applied to biological variables only and biological and environmental variables together. Data of individual abundance, after a log(x + 1) transformation and the calculation of the Bray-Curtis matrix, were classified using the Cluster Analysis and ordinated by means of the Non-Metric Multidimensional Scaling (MDS) technique, in accordance with the strategies used in the study of multispecies distributions. Finally, biological and environmental variables were analysed together by means of correlation matrices and the Principal Component Analysis.  相似文献   

泛大陆的具有两种机制的两阶段分裂作用     

Hynes  A 朱起煌 《海洋地质译丛》1991,(1):37-41

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更新世末—全新世日本海南部和邻近陆地的自然介质变化     

В.  НБ 李寿田 《海洋地质译丛》1993,(4):68-74

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胶州湾贻贝苗源的发展     

张福绥  楼子康  马江虎  于硕恩  刘祥生  李淑英 《海洋科学集刊》1984,(22):115-126

贻贝(Mytilus edulis L.)是一种冷水性双壳类软体动物，它在我国沿岸分布的南限是胶州湾，但仅见于港内码头及船底上，且数量不多。 1958年青岛市水产局、原青岛市海水养殖场和中国科学院海洋研究所等单位曾一起自大连和烟台移贻贝苗至青岛试养，次年养殖架上也曾有少量幼苗附着，但未能大量繁殖起来。1971年后胶南县海水养殖试验场及青岛市第二海水养殖场等先后进行数十亩乃至数百亩移苗养殖，附苗量虽有所增长，但直至1974年仍缺乏生产意义。这种情况引起我们的思考:胶州湾贻贝苗源能否大量发展？它的限制因素是什么？ 1972年至1973年，我们与烟台地区海水养殖试验场等单位曾对烟台沿岸贻贝苗源进行调查研究，确认当时烟台苗种产量不高的主要原因是附着基不足，其次是亲贝不足，据此试验成功了“废旧草绠采苗法”。之后，随着采苗器材的增加及养殖面积的不断扩大，烟台芝罘湾已发展成为一个较好的苗场，通常可供万亩以上生产用苗。这个经验对我们开发胶州湾的苗源很有启发。 1975年胶南县在胶州湾大力推广“废旧草绠采苗法”，收割海带后保留废旧海带架两万台采贻贝苗， 1976年进一步扩大贻贝养殖面积，继续留绠采苗。我们在青岛市水产局及胶南县水产局等单位的协助下，于1975年9月及1976年8-9月，对胶州湾内及湾口(青岛前海)留绠采苗的情况进行了调查，这些调查资料即为本文的主要依据。  相似文献   

海洋复杂体系氧化还原状态的描述与确定及独立电对概念的提出     

洪家珍  李法西 《海洋学研究》1983,(1)

木文提出海水氧化还原体系状态的性质取决于其主要独立电对的氧化还原性质。并测定了某些海洋沉积物样品的Eh、pH、酸挥发性硫及其间隙水中氨等参数,以所得数据为例进行讨论、归纳和推理,得到了一些经验性的结论。图5,表3,参考文献13。  相似文献   

洋脊的岩浆房有多大？     

Nicol.  A 陶鲁 《海洋地质译丛》1993,(3):75-78

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Measurement of DNA single-strand breaks in gill and hemolymph cells of mussels, Mytilus sp., collected on the French Atlantic Coast     

Akcha F  Tanguy A  Leday G  Pelluhet L  Budzinski H  Chiffoleau JF 《Marine environmental research》2004,58(2-5):753-756

DNA single-strand breaks were measured by the comet assay in both gill and hemolymph cells of mussels collected in 3 sampling areas of the French coast (Pointe du Castelli, Pen Bron and Saint-Nazaire Harbour). Whole mussel tissue samples were also collected for the chemical determination of PAH, PCB and heavy metal concentrations. In mussel, a higher level of DNA strand breaks was measured in gill than in hemolymph cells (p < 0.01). Despite a factor of contamination from 2 to 3 between sites, no difference in the extent of mussel DNA strand breaks was shown between sampling locations (p > 0.05), questioning the sensitivity of the assays used in biomonitoring studies.  相似文献