Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis     

Jonsson H  Sandnes KV  Schiedek D  Schneider R  Grøsvik BE  Goksøyr A 《Marine environmental research》2004,58(2-5):655-658

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of approximately 49 kDa size, and weak signals for proteins of approximately 41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of approximately 56 and 59 kDa size, and also a weak immunoreaction with a protein of approximately 43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14-40 ng/g DW in controls to 373-638 ng/g DW in exposed mussels.  相似文献   

烟台海胆体内的纤毛虫和太平洋海胆体内纤毛虫的地理分布     

A.斯特列科夫 《海洋与湖沼》1959,2(1):61-67

1957年夏,中苏海洋动物综合考察队在黄海沿岸烟台地区工作时,参加考察队的阿·阿·斯特列科夫教授采集了海胆肠内的纤毛虫,并以此作为这篇文章的材料。在本文内将提出所见到的纤毛虫名称,并分析它们在太平洋地理分布的情况。  相似文献   

Macrofauna impact on Ulva rigida C. Ag. production and relationship with environmental variables in the lagoon of Venice   总被引：2，自引：0，他引：2  

Balducci C  Sfriso A  Pavoni B 《Marine environmental research》2001,52(1):27-49

The grazing pressure of the macrofaunal invertebrates associated with the biomass of Ulva rigida in the Venice lagoon, their species composition and relationship with environmental variables have been studied. Fifteen sampling campaigns were carried out during different seasons both in the central basin of the lagoon, dominated by macroalgae (especially U. rigida C. Ag.) and in the southern basin, prevalently populated by seagrasses (especially Zostera marina L.). Replicate experiments were conducted in the field by exposing Ulva fronds in net cages of 10 and 1 mm (control) mesh-sizes to allow or prevent grazer entrance. The grazing pressure was determined as Ulva growth rate difference in the cages. In the absence of invertebrate herbivores, Ulva exhibited per cent relative growth rates (%RGRs) ranging from 1.5 to 9.5% day(-1), whereas in their presence the %RGRs were significantly lower (from -2.5 to 3.4% day(-1)) and frequently negative, especially in the station dominated by macroalgae. In this area, peak grazing rates and macrofauna biomasses of up to 8.6% day(-1) and 1,480 g m(-2) fwt (84.4 ash-free dry weight), respectively, were found. On the whole, during in field experiments in the Ulva-dominated station, herbivores removed an amount of biomass whose percentage ranged from 59 to 165% (mean: 103%) of the biomass yield (grazers excluded) found in the cages. These results suggest the possibility that grazers could act as an important factor affecting Ulva production in the Venice lagoon. Macrofauna populations were analysed by means of multivaliate techniques applied to biological variables only and biological and environmental variables together. Data of individual abundance, after a log(x + 1) transformation and the calculation of the Bray-Curtis matrix, were classified using the Cluster Analysis and ordinated by means of the Non-Metric Multidimensional Scaling (MDS) technique, in accordance with the strategies used in the study of multispecies distributions. Finally, biological and environmental variables were analysed together by means of correlation matrices and the Principal Component Analysis.  相似文献   

泛大陆的具有两种机制的两阶段分裂作用     

Hynes  A 朱起煌 《海洋地质译丛》1991,(1):37-41

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洋脊的岩浆房有多大？     

Nicol.  A 陶鲁 《海洋地质译丛》1993,(3):75-78

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Measurement of DNA single-strand breaks in gill and hemolymph cells of mussels, Mytilus sp., collected on the French Atlantic Coast     

Akcha F  Tanguy A  Leday G  Pelluhet L  Budzinski H  Chiffoleau JF 《Marine environmental research》2004,58(2-5):753-756

DNA single-strand breaks were measured by the comet assay in both gill and hemolymph cells of mussels collected in 3 sampling areas of the French coast (Pointe du Castelli, Pen Bron and Saint-Nazaire Harbour). Whole mussel tissue samples were also collected for the chemical determination of PAH, PCB and heavy metal concentrations. In mussel, a higher level of DNA strand breaks was measured in gill than in hemolymph cells (p < 0.01). Despite a factor of contamination from 2 to 3 between sites, no difference in the extent of mussel DNA strand breaks was shown between sampling locations (p > 0.05), questioning the sensitivity of the assays used in biomonitoring studies.  相似文献   

海洋微生物学的主要任务     

A.E.科利斯 《海洋与湖沼》1958,1(2):337-343

海——微生物栖息的特殊环境。特殊的盐类成分,低温,高压,低浓度的有机物质,植物及动物区系的稀少等组成了微生物生命活动的广阔海洋区域一些主要生态特征。 海洋中最初的微生物学研究,早在上一世纪就已进行了,但足它们局限于较狭小的任务——  相似文献   

A NEW MODEL FOR PEBBLE BEACH TRACER DISPERSAL     

A. T. WILLIAMS 《海洋学报(英文版)》1987,6(2):229-234

A series of eight tracer experiments were carried out over one year (1983) on a low energy, macro-tidal beach in South Wales, UK. Tracer movement was monitored and 30 pebbles adjacent to coloured tracer pebbles were measured. Measurements were of the A, B, and C axes, roundness, Maximum Projection Sphericity and Oblate-Prolate indices. Analysis shows that through time, the percentage rejection of these properties increased. It is theorised that the surface tracers are 'misfits' or 'rejects' which have not found their equilibrium position in the pebble facies.  相似文献   

加拿大英属哥伦比亚Wrangellia　Terrain上三叠纪地层中复杂纤维状方解石脉的形成作用     

曲高升 《海洋地质前沿》1996,(10)

加拿大英属哥伦比亚ＷｒａｎｇｅｌｌｉａＴｅｒｒａｉｎ上三叠纪地层中复杂纤维状方解石脉的形成作用Ｉ．Ｓ．Ａｌ－Ａａｓｍ等本文提出一种在中生代早期与早期火成岩密切相关的热水溶液在浅掩埋期间由于有机质和无机质相互作用完成的交代假说。英属哥伦比亚上三叠纪泥岩...  相似文献   

西南太平洋彭林海盆表层和埋藏锰结核成分和生长史     

A．Usui单连芳  刘季花 《海洋地质前沿》1994,(9):11-13

本文所研究的样品由《Hakurei—maru》号科学考察船进行的GH80—1和GH83—3两个航次所采集．这两次航行的目的是作为日本“中太平洋锰结核调查”五年计划(1979—1983)任务的一部分，在威克-塔希提横断层上进行地质调查(GSJ)。  相似文献