Enhanced a novel β-agarase production in recombinant Escherichia coli BL21(DE3) through induction mode optimization and glycerol feeding strategy     

CHAN Zhuhua  CHEN Xinglin  HOU Yanping  GAO Boliang  ZHAO Chungui  YANG Suping  ZENG Runying 《海洋学报(英文版)》2018,(2)

Agarases are hydrolytic enzymes that act on the hydrolysis of agar and have a broad range of applications in food,cosmetics and pharmaceutical industries. In this study, a glycerol feeding strategy based on induction mode optimization for high cell density and β-agarase production was established, which could effectively control acetate yield. First, exponential feeding strategy of glycerol with different overall specific growth rates(μ) was applied in the pre-induction phase. The results showed that the low μ(μ=0.2) was suggested to be the optimal for cell growth and β-agarase production. Second, the effects of induction temperature and the inducer concentration on cell growth and β-agarase production were investigated in the post-induction phase. When induced by isopropyl-β-d-thiogalactoside(IPTG), the strategy of 0.8 mmol/L IPTG induction at 20℃ was found to be optimal for β-agarase production. When cultivation was induced by continuous lactose feeding strategy of 1.0 g/(L·h), the β-agarase activity reached 112.5 U/mL, which represented the highest β-agarase production to date.Furthermore, the β-agarase was capable of degrading G. lemaneiformis powder directly to produce neoagarooligosaccharide, and the hydrolysates were neoagarotetraose(NA4) and neoagarohexaose(NA6). The overall research may be useful for the industrial production and application of β-agarase.  相似文献   

前陆盆地挠曲沉降正演模型的设计与实现——以库车古近纪陆内前陆坳陷沉降分析为例   总被引：2，自引：0，他引：2  

张燕梅  刘景彦  陈清清  张冰 《地球学报》2007,28(1):92-96

本文以二维薄板弹性挠曲模型为基础,对模型进行简化和参数优化选取,并在VC++6.0环境下采用Windows视窗设计,实现了前陆盆地挠曲沉降的正演模拟系统.应用这一系统模拟分析了库车古近纪-新近纪前陆盆地的挠曲沉降过程.模拟结果表明,库车古近纪-新近纪前陆盆地的沉降曲线总体呈上凸型,具有典型前陆盆地沉降的特点,根据沉降速率变化可以把整个沉降过程分为四个部分,分别对应盆地的四个构造层序发育阶段,每一阶段呈不同的沉降特征,相邻两阶段之间存在一盆地回弹隆升、逆冲构造活动终止期,盆地遭受广泛剥蚀形成两个构造层序之间的不整合界面.正演模拟通过将沉降过程时间离散,精细设定不同时间段的构造负载,能够精细恢复前陆盆地的沉降过程和演化.  相似文献   

On the Occurrence of Neuropteris gigantea Sternb. in Kiangsi     

H. C. SZE  KOUTA CHAN 《地质学报》1942,(Z2):195-202

正 In studying the Carboniferous stratigraphy, one is always struck by the fact that those Namurian and Westphalian plants, abundant in other Arcto-  相似文献   

香港与珠三角地区海陆联合地震探测的数据处理   总被引：8，自引：4，他引：8  

夏少红  丘学林  赵明辉  叶春明  陈营华  徐辉龙  王平 《热带海洋学报》2007,26(1):35-38

介绍了香港与珠三角地区海陆联测地震数据处理的一般流程及方法,讨论了导航数据记录时间存在误差的问题及其解决方法,对数据处理程序进行了改进,使之更加通用化。提出了数据处理中需要改进之处,对较远的地震台站采用滤波、均衡、剪切等处理手段,突出弱震相,压制强噪音。广东和香港地区的大部分固定台站均可清晰识别出有效信号,这是首次利用固定台站记录到远距离海上气枪信号,最远接收距离达到240多公里,获得了较好的观测结果。  相似文献   

AN OPERATIONAL STATISTICAL SCHEME FOR TROPICAL CYCLONE INDUCED RAINFALL FORECAST     

李晴岚  兰红平  陈仲良  曹春燕  李程  王兴宝 《热带气象学报(英文版)》2015,21(2):101-110

A non-parametric method is used in this study to analyze and predict short-term rainfall due to tropical cyclones(TCs) in a coastal meteorological station. All 427 TCs during 1953-2011 which made landfall along the Southeast China coast with a distance less than 700 km to a certain meteorological station- Shenzhen are analyzed and grouped according to their landfalling direction, distance and intensity. The corresponding daily rainfall records at Shenzhen Meteorological Station(SMS) during TCs landfalling period(a couple of days before and after TC landfall) are collected. The maximum daily rainfall(R-24) and maximum 3-day accumulative rainfall(R-72) records at SMS for each TC category are analyzed by a non-parametric statistical method, percentile estimation. The results are plotted by statistical boxplots, expressing in probability of precipitation. The performance of the statistical boxplots is evaluated to forecast the short-term rainfall at SMS during the TC seasons in 2012 and 2013. Results show that the boxplot scheme can be used as a valuable reference to predict the short-term rainfall at SMS due to TCs landfalling along the Southeast China coast.  相似文献   

The effects of Southeast Asia fire activities on tropospheric ozone, trace gases and aerosols at a remote site over the Tibetan Plateau of Southwest China   总被引：1，自引：0，他引：1  

C. Y. CHAN  K. H. WONG  Y. S. LI  L. Y. CHAN  X. D. ZHENG 《Tellus. Series B, Chemical and physical meteorology》2006,58(4):310-318

  相似文献   

Fluvial-aeolian interactions: Part II, ancient systems     

R. P. LANGFORD  M. A. CHAN 《Sedimentology》1989,36(6):1037-1051

An understanding of fluvial-aeolian deposition derived from modern case-examples in a previous study is applied to the Permian Cutler Formation and Cedar Mesa Sandstone on the Colorado Plateau. These formations supply an excellent three-dimensional exposure of intertonguing fluvial and aeolian strata. Four distinct facies associations form the bulk of the Cutler Formation and Cedar Mesa Sandstone: (1) aeolian dune deposits; (2) wet interdune deposits; (3) fluvial channel deposits; and (4) overbank-interdune deposits. In addition, two distinctive types of erosion surfaces are found within the Cutler Formation and Cedar Mesa Sandstone: pebble- to granule-rich erosion surfaces (aeolian deflation surfaces) and flood surfaces. Fluvial and aeolian intertonguing result in extensive tabular sheets of aeolian sandstone separated by flood surfaces and overbank-interdune deposits. Fluvial channels are associated with the deposits overlying flood surfaces and are incised into the underlying aeolian sandstones. Overbank-interdune deposits and wet interdune deposits cover flood surfaces and intertongue with overlying aeolian sandstones. The primary characteristics of ancient fluvial-aeolian deposition are overbank-interdune deposits and pronounced extensive erosion surfaces (flood surfaces), which are parallel to underlying fluvial sandstones and thus trend parallel to the palaeoslope and palaeohydrological gradient.  相似文献   

A SENSITIVE AUTOMATED METHOD FOR DETERMINATION OF MERCURY IN GEOLOGICAL MATERIALS BY COLD VAPOR ATOMIC ABSORPTION     

Chris C.Y. CHAN  Sasan BINA 《Geostandards and Geoanalytical Research》1989,13(1):181-186

An improved sensitive automated method for the determination of mercury in geological materials by cold vapor atomic absorption has been developed. Sample is digested with nitric and hydrochloric acids at low heat. Mercuric ions in the digested sample solution are reduced to elemental mercury by stannous chloride. The gaseous mercury separated from the solution is swept by a stream of argon into the absorption cell of an LDC/Milton Roy Mercury Monitor where the atomic absorption at 253.7 nm is measured. The precision of the method is 2.7% r.s.d., and the detection limit is 1 ppb. The results on 53 international geochemical reference samples are presented.  相似文献   

在特殊疫情背景下《城市地理资讯学》MOOC课程教学实施效果分析     

廖萱  陈家朗  郭彦彤  甘振扬  黄文声 《测绘地理信息》2021,46(2):123-126

在新冠肺炎疫情期间,线上教学大规模开展,相关线上教学问题逐渐成为研究热点。传统的测绘地理类课程的教学方式主要是上课时以老师传授知识为主,课后以学生完成作业为辅的方式进行。但是,这种教学方式比较单一,并且在科学化考核和促进学生学习主动性等方面存在诸多问题。然而,线上网络课程是通过互联网以电子设备为教学平台,具有不受教学时空限制、教学方法灵活多样和丰富学生的学习方式等优点。因此,对《城市地理资讯学》MOOC(massive online open courses)课程实施效果进行分析,为其他测绘地理类网络课程设计提供优化思路,同时为无法进行面授教学的特殊时期提供一种有效的教学方案。  相似文献   

NUMERICAL SIMULATION ON THE WIND FIELD STRUCTURE OF A MOUNTAINOUS AREA BESIDE SOUTH CHINA SEA DURING THE LANDFALL OF TYPHOON MOLAVE     

李磊  陈柏纬  胡非  张立杰  柳艳香 《热带气象学报(英文版)》2014,20(1):66-73

Leveraging the commercial CFD software FLUENT, the fine-scale three-dimensional wind structure over the Paiya Mountains on the Dapeng Peninsula near Shenzhen, a city on the seashore of South China Sea, during the landfall of Typhoon Molave has been simulated and analyzed. Through the study, a conceptual wind structure model for mountainous areas under strong wind condition is established and the following conclusions are obtained as follows： （1） FLUENT can reasonably simulate a three-dimensional wind structure over mountainous areas under strong wind conditions; （2） the kinetic effect of a mountain can intensify wind speed in the windward side of the mountain and the area over the mountain peak; and （3） in the leeward side of the mountain, wind speed is relatively lower with relatively stronger wind shear and turbulence.  相似文献