Use of immunofluorescence technique in cultured fibroblasts from Mediterranean cetaceans as new "in vitro" tool to investigate effects of environmental contaminants     

Marsili L  Casini S  Bucalossi D  Porcelloni S  Maltese S  Fossi MC 《Marine environmental research》2008,66(1):151-153

The aim of the present study was to propose the immunofluorescence technique in cultured fibroblasts from Mediterranean cetaceans as a new "in vitro" tool to explore the susceptibility of these marine mammals to different xenobiotic compounds. The cell lines were cultured from integument biopsies of free-ranging and stranded cetaceans (dead within 12h). Using the indirect immunofluorescence assay, we detected endogenous proteins induced by different contaminants. Here we present the method used for qualitative and quantitative evaluation of cytochromes P450 (CYP1A1 and CYP2B) induced by some POPs (DDTs and PCBs) and emerging contaminants (PBDEs) in fibroblast cell cultures of striped dolphin (Stenella coeruleoalba) and bottlenose dolphin (Tursiops truncatus). Immunofluorescence was quantified with a specially designed Olympus macro, DetectIntZ. A major result was the possibility of using this "in vitro" assay to quantify induction of endogenous proteins.  相似文献   

Ka波段毫米波云雷达对青藏高原东南缘降水回波的分析     

王卫民  徐八林  雷勇  舒斌  马芳 《气象》2024,50(3):291-302

利用丽江站新建的Ka波段毫米波云雷达获得的高时间分辨率的垂直观测资料,结合同址的地面自动气象站和雨滴谱的分钟数据、常规探空数据和附近C波段天气雷达的强度回波,分析了两次降水过程前后云雷达反射率因子Z、径向速度V_r、速度谱宽S_w的垂直变化规律。分析表明:在发生弱降水时,云雷达Z在垂直方向的变化不明显;但V_r、S_w值在0℃层稍低位置有一个明显的分界层(融化层),粒子通过融化层后V_r、S_w都是快速变大,这个变化主要是粒子的相态由固态变成液态引发的,可以通过V_r、S_w突变值的位置来识别0℃层亮带的高度。从C波段天气雷达回波强度、剖面图及云雷达位置的时间-高度图看,对毛毛雨和小雨的回波,强度和高度差异比较明显,毛毛雨比小雨回波高度低、强度弱,与云雷达相比C波段雷达对高一些的云观测不到,对距离较远的弱降水回波无法观测到;由于相同粒子对不同波长电磁波的散射不一样,造成两种雷达垂直方向观测到的Z变化不同。对比弱降水回波,云雷达在...  相似文献   

Europe adapts to climate change: Comparing National Adaptation Strategies     

G. Robbert Biesbroek  Rob J. Swart  Timothy R. Carter  Caroline Cowan  Thomas Henrichs  Hanna Mela  Michael D. Morecroft  Daniela Rey 《Global Environmental Change》2010,20(3):440-450

For the last two decades, European climate policy has focused almost exclusively on mitigation of climate change. It was only well after the turn of the century, with impacts of climate change increasingly being observed, that adaptation was added to the policy agenda and EU Member States started to develop National Adaptation Strategies (NASs). This paper reviews seven National Adaptation Strategies that were either formally adopted or under development by Member States at the end of 2008. The strategies are analysed under the following six themes. Firstly, the factors motivating and facilitating the development of a national adaptation strategy. Secondly, the scientific and technical support needed for the development and implementation of such a strategy. Thirdly, the role of the strategy in information, communication and awareness-raising of the adaptation issue. Fourthly, new or existing forms of multi-level governance to implement the proposed actions. Fifthly, how the strategy addresses integration and coordination with other policy domains. Finally, how the strategy suggests the implementation and how the strategy is evaluated. The paper notes that the role of National Adaptation Strategies in the wider governance of adaptation differs between countries but clearly benchmarks a new political commitment to adaptation at national policy levels. However, we also find that in most cases approaches for implementing and evaluating the strategies are yet to be defined. The paper concludes that even though the strategies show great resemblance in terms of topics, methods and approaches, there are many institutional challenges, including multi-level governance and policy integration issues, which can act as considerable barriers in future policy implementation.  相似文献   

Crystal-chemistry of clinopyroxenes from potassic and ultrapotassic rocks in central Italy: implications on their genesis   总被引：2，自引：0，他引：2  

Daniela Cellai  Sandro Conticelli  Silvio Menchetti 《Contributions to Mineralogy and Petrology》1994,116(3):301-315

The clinopyroxenes mentioned have been investigated by single crystal X-ray diffraction combined with electron microprobe analysis. The aim of this study was to characterize the crystal-chemical variations of clinopyroxenes in order to delineate the intracrystalline constraints which are characteristic of specific magmatic environments. Clinopyroxenes (cpx) crystallized from peralkaline ultrapotassic melt with kamafugitic and lamproitic affinities are characterized by high Si contents, which are insensitive to variations in silica abundance and silica saturation of the melt. The high Si occupancy in clinopyroxenes from kamafugitic magma is coupled to large M1 (i.e. Mg and Fe²⁺) and M2 (high Ca occupancy) sites, whereas in clinopyroxenes from magmas with lamproitic affinity, high Si content is combined with large M1 but small M2 sites. Clinopyroxenes from Romantype alkaline potassic and ultrapostassic rocks are characterized by an expanded tetrahedron (high ^IVA1 content) and small M1 site which is combined with small M2 polyhedron in clinopyroxenes from the potassic rocks and large M2 site in those from the ultrapotassic rocks.  相似文献   

Comparison between Pollutant Concentration in the Samples of Fog and Rime Water Collected at Mt. Milešovka     

Fišák  Jaroslav  Řezáčová  Daniela 《Studia Geophysica et Geodaetica》2001,45(3):319-324

The article aims at showing the differences in concentration of pollutants that are contained in the samples of fog and/or low cloud water in comparison with the water from rime. The results follow from the fog and rime measurements made at Mt. Mileovka (eské Stedohoí Mountains). They are compared with the results of other studies that also report the differences in fog and rime chemistry.  相似文献   

Equilibrium and disequilibrium degassing of a phonolitic melt (Vesuvius AD 79 “white pumice”) simulated by decompression experiments     

Giada Iacono Marziano  Burkhard C. Schmidt  Daniela Dolfi 《Journal of Volcanology and Geothermal Research》2007

Equilibrium and disequilibrium degassing of a volatile phase from a magma of K-phonolitic composition was investigated to assess its behavior upon ascent. Decompression experiments were conducted in Ar-pressurized externally heated pressure vessels at superliquidus temperature (1050 °C), in the pressure range 10–200 MPa using pure water as fluid phase. All experiments were equilibrated at 200 MPa and then decompressed to lower pressures with rates varying from 0.0028 to 4.8 MPa/s. Isobaric saturation experiments were performed at the same temperature and at 900–950 °C to determine the equilibrium water solubility in the pressure range 30–250 MPa. The glasses obtained from decompression experiments were analyzed for their dissolved water content, vesicularity and bubble size distribution. All decompressed samples presented a first event of bubble nucleation at the capsule–melt interface. Homogeneous bubble nucleation in the melt only occurred in fast-decompressed experiments (4.8 and 1.7 MPa/s), for ΔP ≅ 100 MPa. For these decompression rates high water over-saturations were maintained until a rapid exsolution was triggered at ΔP > 150 MPa. For slower rates (0.0028, 0.024, 0.17 MPa/s) the degassing of the melt took place by diffusive growth of the bubbles nucleating at the capsule–melt interface. This process sensibly reduced water over-saturation in the melt, preventing homogeneous nucleation to occur. For decompression rates of 0.024 and 0.17 MPa/s low water over-saturations were attained in the melt, gradually declining toward equilibrium concentrations at low pressures. A near-equilibrium degassing path was observed for a decompression rate of 0.0028 MPa/s. Experimental data combined with natural pumice textures suggest that both homogeneous and heterogeneous bubble nucleations occurred in the phonolitic magma during the AD 79 Vesuvius plinian event. Homogeneous bubble nucleation probably occurred at a depth of ∼ 3 km, in response to a fast decompression of the magma during the ascent.  相似文献   

Geodynamic implications of deep mantle upwelling in the source of Tertiary volcanics from the Veneto region (South-Eastern Alps)     

Patrizia Macera  Daniela Gasperini  Claudia Piromallo  Janne Blichert-Toft  Delphine Bosch  Aldo Del Moro  Silvana Martin 《Journal of Geodynamics》2003,36(5):563

Major and trace element and Sr–Nd–Hf–Pb isotopic data for the most primitive Tertiary lavas from the Veneto region (South-Eastern Alps, Italy) show the typical features of HIMU hotspot volcanism, variably diluted by a depleted asthenospheric mantle component (⁸⁷Sr/⁸⁶Sr_i=0.70306–0.70378; _Ndi=+3.9 to +6.8; _Hfi=+6.4 to +8.1, ²⁰⁶Pb/²⁰⁴Pb_i=18.786–19.574). P-wave seismic tomography of the mantle below the Veneto region shows the presence of low-velocity anomalies at depth, which is consistent with possible upwellings of plume material. Between the depths of 100–250 km the velocity anomalies are approximately 2–2.5% slower than average, implying a temperature excess of about 220–280 K, in agreement with estimates for other mantle plumes in the world. In this context, the Veneto volcanics may represent the shallow expression of a mantle upflow. The presence of a HIMU-DM component in a collision environment has significant geodynamic implications. Slab detachment and ensuing rise of deep mantle material into the lithospheric gap is proposed to be a viable mechanism of hotspot magmatism in a subduction zone setting.  相似文献   

Magnetic data analysis at low latitudes using magnitude transforms   总被引：2，自引：0，他引：2  

Daniela Gerovska  Petar Stavrev 《Geophysical Prospecting》2006,54(1):89-98

Magnitude transforms include magnitude magnetic anomalies (MMA), their gradients and Laplacians. They can be calculated from the total magnetic anomalies or other component anomalies. Magnitude magnetic anomalies have a space distribution different from that of the component anomalies. Their values are non‐negative and their respective patterns are similar to the positive gravity anomalies. Magnitude transforms are an effective tool for magnetic data analysis due to their simplified pattern and direct correlation with the space location of the source. They have advantages over the traditionally used reduction‐to‐the‐pole (RTP) transform, especially at low magnetic latitudes. The calculation of magnitudes of the anomalous field requires the total field data to be transformed into the component anomalies, while the reduction‐to‐the‐pole transform also includes a rotation of the magnetization vector, the orientation of which is usually assumed. For equal latitudes, the transfer functions of component‐component transforms in the frequency domain show better stability than the component‐component‐rotation transfer function. This is illustrated by a comparison of analytical expressions, and synthetic models of magnetic fields. The Dixon seamount case shows the possibilities for an improved data analysis and more confident source recognition at low latitudes using magnetic transforms.  相似文献   

高山雷达站选址对估测降水的影响分析   总被引：1，自引：0，他引：1  

徐八林  刘黎平杨明张万诚  陈兆武 《气象科技》2006,34(3):i0003-i0004

高山雷达站天线高度对雷达的垂直探测范围影响较大,加之中小尺度灾害性天气演生、发展的关键区域在高度1500~4500 m左右,所以高山雷达站在选址时应考虑当地零度层平均高度和雷达的垂直探测范围。通过定量估测层状云降水的个例实践,发现探测高度会限制定量估测降水,探测高度对近距离降水估测影响较小,但对远距离降水估测影响较大,原因主要是雷达的垂直探测范围及当地零度层亮带高度的影响。故在高山雷达站选址中应考虑雷达的垂直探测范围及当地零度层亮带高度的影响,同时对已建设完成的高山天气雷达应考虑对高山雷达做0°仰角的观测可行性研究。  相似文献   

一次孟加拉湾风暴和冷空气影响下滇西大暴雨中尺度分析   总被引：8，自引：1，他引：8       下载免费PDF全文

鲁亚斌张腾飞  徐八林杨明周国莲  索渺清寸灿琼 《应用气象学报》2006,17(2):201-206

应用MICAPS资料, 通过天气诊断分析, 结合FY-2卫星云图及德宏CINRAD-CC雷达体扫资料, 分析了发生在2004年5月18日滇西地区的大暴雨过程。发现本次大暴雨过程天气尺度影响系统为初夏孟加拉湾风暴及南下冷锋切变; 大暴雨发生在高能高湿的水汽辐合中心、700 hPa螺旋度正值区及湿 Q 矢量散度大值辐合区内; 卫星云图上, 多个β-中尺度对流系统在大暴雨区发展; 多普勒雷达回波为絮状混合型降水回波, 强度在30~44 dBz之间, 频繁出现的逆风区、低空急流、中尺度辐合线等中小尺度系统是造成本次大暴雨的直接影响系统。  相似文献