Glutathione-mediated chlorothalonil detoxification in channel catfish gills     

Evan P. Gallagher  Richard T. Di Giulio 《Marine environmental research》1992,34(1-4)

Maintenance of the glutathione (GSH) pathway is critical in guarding against the toxic effects of a variety of pollutants. However, despite their importance as an initial line of defense against waterborne toxicants, little is known regarding the role of fish gills in GSH-mediated xenobiotic metabolism. In these studies, the role of channel catfish gills in the detoxification of 2,4,5,6-tetrachloroisophthalonitrile (chlorothalonil), an electrophilic fungicide, was examined. Chlorothalonil was metabolized in vitro by gill cytosolic and microsomal glutathione S-transferases (GSTs). Chlorothalonil metabolism by cytosolic fructions was reduced markedly when GSH was omitted from reaction mixtures. Microsomal metabolism of chlorothalonil did not occur in the absence of GSH and in the presence of an NADPH-regenerating system lacking GSH. Channel catfish exposed in vivo to chlorothalonil had increased gill GSH and cysteine concentrations after 72 h exposure, and increased gill gamma-glutamylcysteine synthetase (GCS) activity at 144 h of exposure. Our results show that the gills play an important role in the metabolism and detoxification of chlorothalonil in the channel catfish. Catfish can maintain elevated gill GSH concentrations in the presence of chlorothalonil by increasing gill cysteine concentrations.  相似文献   

The State of the Adriatic Sea Centered on the Small Pelagic Fish Populations   总被引：1，自引：0，他引：1  

Massimo Azzali  rea De  Felice  Manlio Luna  Giulio Cosimi  Flavio Parmiggiani 《Marine Ecology》2002,23(S1):78-91

Abstract. The paper provides an overview of the state of the small pelagic fish in the Adriatic Sea. The North Adriatic is reviewed for the 1976–1998 period and the South Adriatic for the 1987–1998 period. First the fluctuations in time and in space of the pelagic biomass, as a whole and per species, are presented. Then the dramatic collapse of anchovy stock and its apparent association with the decrease of surface temperature is discussed. Finally the changes of the anchovy and sardine catches are compared and analysed with respect to their abundances in the sea. The conclusion is that acoustic and satellite methods are able to provide a large amount of information on the variability in the pelagic populations; this is essential for an appropriate management of these resources. There is still a need, however, to refine the methods and to integrate this kind of information with further environmental data (including natural predators).  相似文献   

CYP1B1 knockdown does not alter synergistic developmental toxicity of polycyclic aromatic hydrocarbons in zebrafish (Danio rerio)     

Timme-Laragy AR  Noyes PD  Buhler DR  Di Giulio RT 《Marine environmental research》2008,66(1):85-87

Polycyclic aromatic hydrocarbons (PAHs) are contaminants increasing in the environment largely due to burning of fossil fuels. Our previous work identified a synergistic toxicity interaction in zebrafish embryos occurring when PAHs that are agonists for the aryl hydrocarbon receptor (AHR) co-occur with PAHs that are CYP1A inhibitors. This toxicity is mediated by the AHR2, and morpholino knockdown of CYP1A exacerbated toxicity. This study tested two hypotheses: (1) in the absence of functional CYP1A, metabolism of PAHs is shunted towards CYP1B1, which has been shown in mammals to produce more reactive metabolites of PAHs; alternatively, (2) CYP1B1 serves a protective role similar to CYP1A. We used a morpholino approach to knockdown CYP1B1 alone and in co-knockdown with CYP1A to determine whether we could alter deformities caused by synergistic toxicity of PAHs. CYP1B1 knockdown was not different from non-injected controls; nor were CYP1B1+CYP1A co-knockdown deformities different from CYP1A knockdown alone. These data suggest that CYP1B1 is not a significant factor in causing synergistic toxicity of PAHs, nor, in contrast to CYP1A, in providing protection.  相似文献   

The Central Main Ethiopian Rift is younger than 8 Ma: confirmation through apatite fission‐track thermochronology     

Tsegaye Abebe  Maria Laura Balestrieri  Giulio Bigazzi 《地学学报》2010,22(6):470-476

An isolated block of Precambrian basement rocks and Mesozoic sediments is exposed at Kella along the western margin of the Central Main Ethiopian Rift (MER), surrounded by Tertiary to Quaternary volcanic rocks. Apatite fission‐track thermochronology on two basement samples yielded ages of 7.2 ± 1.0 Ma and 6.7 ± 3.0 Ma and a long mean track length (>14.5 μm). Rapid Late Miocene cooling is attributed to denudation related to rifting. Despite the paucity of data, due to the absence of suitable lithologies in the area, our data confirm that the Central MER is younger than 8 Ma as recently proposed on the basis of field evidence and radiometric dating of volcanics. This implies that the Central MER formed after the Northern MER, indicating a diachronous development of this third arm of the Red Sea–Gulf of Aden–Ethiopian Rift system. Terra Nova, 00, 000–000, 2010  相似文献   

The potassic feldspar in metamorphic rocks from the western Hohe Tauern area,eastern Alps     

Raase  Peter  Morteani  Giulio 《International Journal of Earth Sciences》1976,65(1):422-436

Zusammenfassung Es wurden Kalifeldspäte aus Orthogneisen, Paragneisen und Glimmerschiefern des westlichen Tauemfensters (Hohe Tauern, Tirol) mit optischen und röntgenographischen Methoden im Hinblick auf ihren Strukturzustand untersucht.Es zeigte sich, daß in den Randbereichen des Tauemfensters in Gesteinen der Grünschieferfazies weitgehend geordneter Mikroklin mit einem optischen Achsenwinkel 2 V_x 80°, Auslöschungswinkel Z (010)=15–20°, Doppelbrechung n_z-n_x= 0,0065, Triklinität=0,90-–0,95, ca. 0,88 Al auf T₁O-Position und einem Ab-Gehalt von ca. 5 Gew.% auftritt. Dagegen wurden in Gesteinen der schwachtemperierten Amphibolitfazies des zentralen Bereichs Orthoklase bis intermediäre Mikrokline mit stark schwankenden optischen Eigenschaften, mit Triklinitäten zwischen 0 und 0,7, 0,8 bis 0,9 Al in T₁-Positionen, 0,45–0,8 Al auf T₁O und Ab-Gehalten von ca. 10 Gew.% festgestellt. Die Kalifeldspatkristalle zeigen ungleichmäßige Auslöschung mit Winkeln von Z (010) zwischen 0 und 15°, 2 V_x zwischen 50 und 75° und n_z-n_x=0,005 bis 0,006. Teilweise ist Zonarbau mit geringerer Triklinität am Kornrande erkennbar. Diese undulös auslöschenden Kalifeldspäte enthalten einzelne schmale Lamellen von maximal triklinem Mikroklin mit Z (010)=15–20° und n_z-n_x= 0,006–0,007.Die Grenze zwischen dem Verbreitungsgebiet von maximalem Mikroklin einerseits und Orthoklas bis intermediärem Mikroklin andererseits verläuft in den westlichen Hohen Tauern ungefähr konform mit der Albit-Oligoklas-Grenze (Morteani &Raase, 1974) und mit der aus Sauerstoff-Isotopen-Untersuchungen bestimmten 500° C-Isotherme (Hoernes &Friedrichsen, 1974).

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The potassic feldspar in orthogneisses, paragneisses, and mica schists from the western Tauernfenster (Hohe Tauern, Tyrol) were studied by optical and X-ray methods with regard to their structural state and in relation to grade of metamorphism.In the peripheral region of the Tauernfenster in greenschist-facies rocks highly triclinic microcline occurs with an optic angle 2 V_x of about 80°, extinction angle Z (010) =15–20°, birefringence n_z-n_x=0.0065, triclinicity=0.90–0.95, approx. 0.88 Al in T₁O-site, and an Ab-content of about 5 wt.%. In the central area with rocks of the low-grade amphibolite facies, on the other hand, orthoclase to intermediate microcline with highly variable optical properties, triclinicities in the range of 0–0.7, 0.8–0.9 Al in T₁-sites, 0.45–0.8 Al in T₁O-site, and an Ab-content of about 10 wt.% was recognized. The K-feldspar grains have nonuniform extinction Z (010) scattering in the range of 0–15°, 2 V_x in the range of 50–75°, and n_z-n_x= 0.005–0.006. Sometimes a zonal structure with lower triclinicity exists at the rim. These undulatory K-feldspar grains usually contain small lamellae of highly triclinic microcline with Z (010) =15–20° and n_z-n_x=0.006–0.007.From the occurrence of maximum microcline and of orthoclase to intermediate microcline in the Hohe Tauern area an isograd was defined that is approximately conformable with the albite-oligoclase isograd (Morteani &Raase, 1974) and with the 500° C isotherm based on oxygen isotope analyses (Hoernes &Friedrïchsen, 1974).

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Résumé Les feldspaths potassiques d'orthogneiss, de paragneiss et de micaschistes de la Fenêtre des Tauern occidental (Hohe Tauern, Tirol) ont été étudiés du point de vue de leur état structural.Dans les roches du faciès schistes verts de la région périphérique de la Fenêtre des Tauern les microclines sont caractérisés par un angle des axes optiques 2 V_p =80°, un angle d'extinction n_g (010)=15–20°, une biréfringence n_g-n_p=0,0065, une triclinicité= 0,90–0,95, 0,88 Al dans le site T₁O et par une teneur en albite d'environ 5%. Au contraire, dans les roches du faciès amphibolite à faible degré de la région centrale, ont été observées des orthoses allant à des microclines intermédiaires avec des propriétés optiques très variables, un degré de triclinicité de 0 à 0,7, 0,8–0,9 Al dans les sites T₁, 0,45–0,8 Al dans T₁O et avec une teneur en albite d'environ 10%.Les cristaux de feldspath montrent une extinction irrégulière avec des angles Z (010) variable de 0 à 15°, 2 V_p de 50 à 75° et n_g-n_p=0,005–0,006.On peut voir quelquefois une structure zonée avec une triclinicité moindre au bord des cristaux. Ces feldspaths potassiques contiennent de minces lamelles de microcline à triclinicité maximum, avec n_g (010)=15–20° et n_g-n_p 0,006–0,007.La limite entre les régions à microcline maximum d'une part, et à orthose ou microcline intermédiaire d'autre part, suit, dans les Hohe Tauern, sensiblement l'isograde albite-oligoclase (Morteani &Raase, 1974) et, l'isotherme de 500° C telle qu'elle fut determinée les isotopes de l'oxygène (Hoernes &Friedrichsen, 1974).

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, ( , ) ., 2 V_x80°, Z (010)=15–20°, n_z–n_x=0,0065, =0,90–0,95, 0,88 l T₁O Ab 5 .-%. , 0 0,7, 0,8-0,9 1 T₁ l 10 .-%. Z V (010) 0 15°, 2 V_x 50 75° n_z–n_x=0,005–0,006. . Z V (010) =15-20° n_z–n_x=0,006–0,007. , Morteani & Raase (1974) 500° , Hoernes & Friedrichsen (1974).

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Linear or non-linear rheology in the Earth's mantle: the prevalence of power-law creep in the postglacial isostatic readjustment of Laurentia   总被引：1，自引：0，他引：1  

Paolo Gasperini  Giulio Dal Forno  Enzo Boschi 《Geophysical Journal International》2004,157(3):1297-1302

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Fault plane processes and mesoscopic structure of a strong-type seismogenic fault in tonalites (Adamello batholith, Southern Alps)   总被引：3，自引：3，他引：3  

Giulio Di Toro  Giorgio Pennacchioni   《Tectonophysics》2005,402(1-4):55

The Gole Larghe Fault is an exhumed paleoseismic fault crosscutting the Adamello tonalites (Italian Southern Alps). Ambient conditions of faulting were 9–11 km in depth and 250–300 °C. In the study area the fault accommodates 1100 m of dextral strike-slip over a fault thickness of 550 m. Displacement is partitioned into three hierarchically different sets of discrete subparallel cataclastic horizons (faults_1–2–3). Fault displacement is in the range of few centimeters (faults₃) to a maximum of a few tens of meters in major faults₁. Faults_1–2 nucleated on pre-existing joints, whereas faults₃ are newly generated fractures produced during slip along faults_1–2. Each fault within the Gole Larghe Fault records the same evolution with development of indurated cataclasites precursory to pseudotachylyte production. Pseudotachylytes are usually generated at the host rock/cataclasite boundary and within cataclasites the mean clast size decreases getting closer to pseudotachylyte fault veins. Pseudotachylytes and cataclasites have a similar chemical composition which is enriched in Loss On Ignition, K, Rb, Ba, U and Fe³⁺ compared to host rock.We envision two models for the evolution of the Gole Larghe Fault. In both models synkinematic fluid–rock interaction along a fault causes fault hardening by precipitation of abundant K-feldspar+epidote (and minor chlorite) in the cataclasite matrix conducive to final production of pseudotachylyte. In the first model, induration occurs progressively by differential precipitation related to fabric evolution in cataclasites. In the second model, induration occurs abruptly dependent on the development of full connectivity within the fault network and to fluid reservoir. Whatever the model, the Gole Larghe Fault represents a strong fault, where hardening processes resulted in a low displacement/fault thickness ratio and contrast with many mature weak faults where localized repeated seismic slip along the same weak horizons yields high displacement/fault thickness ratios.  相似文献   

Ab-initio structure, energy and stable Cr isotopes equilibrium fractionation of some geochemically relevant H-O-Cr-Cl complexes     

Giulio Ottonello 《Geochimica et cosmochimica acta》2005,69(4):851-874

Twelve Cr(III) molecules and six Cr(VI) molecules of geochemical interest in the system Cr-H-O-Cl were investigated by ab initio Density Functional Theory in the aim of determining stationary state geometries, electronic energies and vibrational properties. The vibrational analysis conducted on the ⁵⁰Cr, ⁵²Cr, ⁵³Cr and ⁵⁴Cr isotopomers indicates important separative effects which result in mass spectrometric measurable fractionation factors in a wide thermal range. The effect of the central cation on the computed vibrational modes is interpreted in terms of Redlich’s product rule with the introduction of an isotopic ergodicity factor related to the effect of bulk molecular masses and momenta of inertia about the principal rotational axes. After the initial gas phase optimization, some relevant molecules were subjected to the effects of reaction field through Onsager’s Model, Tomasi’s continuum Model and Conductor-like Screening Model procedures. Structures and vibrational properties prove in all cases not to be severely affected by solvation, although the effect of the reaction field is to reduce somewhat the isotopic fractionation in Cr(III)-Cr(VI) aqueous redox equilibria, with respect to the gaseous state. Some of the computed separative effects are applicable to geochemical investigations.  相似文献   

A climatic control on the accretion of meteoric and super-chondritic iridium–platinum to the Antarctic ice cap     

Paolo Gabrielli  John M.C. Plane  Claude F. Boutron  Sungmin Hong  Giulio Cozzi  Paolo Cescon  Christophe Ferrari  Paul J. Crutzen  Jean Robert Petit  Vladimir Y. Lipenkov  Carlo Barbante   《Earth and Planetary Science Letters》2006,250(3-4):459-469

Meteoric smoke particles (MSPs) form through the vaporization of meteoroids and the subsequent re-condensation of metallic species in the mesosphere. Recently, iridium and platinum enrichments have been identified in Greenland ice layers and attributed to the fallout of MSPs supplying polar latitudes with cosmic matter during the Holocene. However, the MSP fallout to Antarctica during the Earth's climatic history remains essentially unknown.

We have determined iridium and platinum in deep Antarctic ice from Dome C and Vostok dated back to 240 kyrs BP. We find high super-chondritic fluxes during warm periods and low meteoric accretion during glacial times, a pattern that is opposite to any known climatic variation in dust fallout to polar regions. The proposed explanation of this accretion regime is a weaker polar vortex during warm periods, allowing peripheral air masses enriched in volcanic iridium and platinum to penetrate inland to Antarctica. The MSP signal emerges only during cold phases and is four times lower than in the Greenland ice cap where more snow accumulates. This suggests that wet deposition is an important route of cosmic material to the Earth's surface.  相似文献   

Energetics of multiple oxides with spinel structure     

Giulio Ottonello 《Physics and Chemistry of Minerals》1986,13(2):79-90

A thermodynamic model for multiple oxides with spinel structure based on the atomistic approach (lattice energy, enthalpy, bulk modulus) and semiempirical estimates (heat capacity functions, entropy, thermal expansion) is presented. The model fits the experimental high temperature free energy values of the reference compounds, with a mean absolute error of 0.65 percent (19 values). The standard state stable configuration of most reference compounds is shown to be achieved at a local minimum in the free energy vs. degree of inversion function. This is interpreted as evidence of internal consistency of the model.  相似文献