Development of a real-time PCR method for Thalassiosira rotula rapid detection     

HE Shanying  YU Zhigang  MI Tiezhu 《海洋学报(英文版)》2007,26(2):133-139

Gene specific primers and DNA probe were designed based on the sequence of 18S rDNA cloned from the red tide alga Thalassiosira rotula. A real-time fluorescent quantitative PCR (RFQ-PCR) method was developed for quantitative detection of T.rotula. The RFQ-PCR assay data showed that the results obtained with the RFQ-PCR quite good agreement with those with the light microscope (LM) counting method, which suggested that the RFQ-PCR could be a useful method for red tide alga detection.  相似文献   

南大洋普里兹湾基于234Th/238U不平衡法的POC输出通量研究   总被引：2，自引：0，他引：2       下载免费PDF全文

何建华  马豪  陈立奇  项宝强  曾宪章  尹明端  曾文义 《海洋学报》2007,29(4):69-76

中国第22次南极科学考察(2005年11月至2006年3月)期间,测定了南极普里兹湾海域5个站位的从表层至150 m水深的不同层位水样中溶解态和颗粒态234Th,238U的放射性比活度以及颗粒有机碳.利用234Th/238U在上层水体中的不平衡,计算了南极普里兹湾上层水体中234Th的平均停留时间和输出通量.结果显示,随着纬度的增加,上层水体中颗粒态和溶解态234Th的平均停留时间总体趋向减小,并在中纬度站位出现了最低值,分别为1~8和29~48 d,而颗粒态和溶解态234Th的输出通量则在中纬度站位出现了最大值,分别为21~38和26~39 dpm/(m3·d).运用箱型清除模式,利用两种不同的方法估算了各水柱中从真光层底部输出的POC通量,平均值分别达到104.7 mmol/(m2·d)(E法)和120.6 mmol/(m2·d)(B法),表明南极普里兹湾夏季存在很高的新生产力,它将会对该海域碳的生物泵过程产生重要作用.  相似文献   

区域海洋地质数据库建设纲要   总被引：5，自引：0，他引：5  

魏合龙  戴勤奋  林峰  王圣洁  何书锋  苏国辉 《海洋地质与第四纪地质》2004,24(1):139-142

区域海洋地质数据库是中国地质调查局基础数据库的一个重要组成部分，是实现地质调查基础性与公益性战略部署的依托。根据区域海洋地质数据库建设的目标与任务，在数据结构和实施步骤等方面进行了初步研究。区域海洋地质数据库及其管理信息系统适合采取基于Web系统的三层体系结构，在数据构成上亦分为区域海洋地质调查原始资料与成果资料，其中元数据库的建设对数据库的应用非常重要，用户可以通过元数据了解数据的存在性、适宜性、有效性等，确定数据的使用方法，因此，元数据库的建设应采用国际已经普遍认可的ISO/TC211发布的ISO19115空间元数据标准。  相似文献   

我国海域分等定级指标体系研究   总被引：3，自引：0，他引：3  

陈明剑  何国祥 《海洋学报》2002,24(3):18-27

长期以来,海域的无偿使用导致海洋开发活动秩序混乱、海洋环境污染加剧、海洋生物资源枯竭、海洋生态环境遭到破坏,严重阻碍和制约沿海地区的可持续发展.本文试图根据经济学的“地租级差”理论和运筹学中的“层次分析法”并结合我国沿海11个省、市和自治区的社会、经济、基础设施、自然资源等因素进行理论探讨,给出了我国管辖海域的等级.为今后我国制定海域有偿使用金的征收标准奠定基础.  相似文献   

盐碱水NaCl浓度和碱度对银鲫(Carassius auratus gibelio)幼鱼毒性的初步研究   总被引：2，自引：0，他引：2  

郑伟刚  张兆琪  张美昭  董双林 《中国海洋大学学报(自然科学版)》2001,31(4):513-517

通过对银鲫(Carassiusauratusgibelio)幼鱼(L=3.56±0.28cm)的NaCl浓度(pH=8.80±0.10)及碱度(pH=8.84±0.26)的急性毒性试验，用概率单位法求得其24，48，96h的半致死浓度(LC50值)NaCl浓度分别为11.53、10.77、8.58g/L；碱度分别为98.74、79.49、64.19mmol/L。在此基础上采用水生毒理联合效应相加指数法进行了氯化钠与碳酸氢钠浓度的联合毒性实验(浓度=11)，结果在96h内二者的关系全部为协同作用,且其协同性逐渐减弱并趋向于相加作用。  相似文献   

A study on remote sensing models of sea ice thickness by microwave radiometry     

Zheng Quan''an  Zhang Dong  Pan Jiayi 《海洋学报(英文版)》1993,12(2):197-206

Extrapolating from the propagation theories of electromagnetic waves in a layered medium, a three-layer medium model is deduced in this paper by using microwave radiometric remote sensing technology which is suitable to first-year sea ice condition of the northern part of China seas. Comparison with in situ data indicates that for microwave wavelength of 10 cm, the coherent model gives a quite good fit result for the thickness of sea ice less than 20 cm, and the incoherent model also works well for thickness within 20 to 40 cm. Based on three theoretical models, the inversion soft ware from microwave remote sensing data for calculating the thickness of sea ice can be set up. The relative complex dielectrical constants of different types of sea ice in the Liaodong Gulf calculated by using these theoretical models and measurement data are given in this paper. The extent of their values is (0. 5-4. 0)-j(0. 07~0. 19).  相似文献   

黄河口及渤海中南部沉积物工程特性及其机理   总被引：3，自引：0，他引：3  

郑继民  沈谓铨  陆念祖  冯秀丽 《中国海洋大学学报(自然科学版)》1994,(2)

采用Ｋａｓｔｅｎ取样器从黄河口及渤海湾中南部海底沉积物中取样，对样品通过土工性质、微结构研究以及粘土矿物分析，阐明本研究区三维空间展布的海底沉积物形成机理及其工程地质规律，论证水动力作用和斜坡不稳定性对工程特性和微结构的重要影响．  相似文献   

Effects of light, temperature and nutrients on photosynthesis of Biddulphia regia     

Li Wenquan  Zheng Airong  Wang Xian  Chen Qinghua 《海洋学报(英文版)》1996,15(1):127-131

Ｅｆｆｅｃｔｓｏｆｌｉｇｈｔ，ｔｅｍｐｅｒａｔｕｒｅａｎｄｎｕｔｒｉｅｎｔｓｏｎｐｈｏｔｏｓｙｎｔｈｅｓｉｓｏｆＢｉｄｄｕｌｐｈｉａｒｅｇｉａＩＮＴＲＯＤＵＣＴＩＯＮＬｉｇｈｔ，ｔｅｍｐｅｒａｔｕｒｅａｎｄｎｕｔｒｉｅｎｔｓａｒｅｉｍｐｏｒｔａｎｔｅ...  相似文献   

An ecological study on the fouling organisms in Yantai Harbour     

Li Chuanyan  Huang Zongguo  Wang Jianjun  Zheng Chengxing  Lin Sheng 《海洋学报(英文版)》1991,10(4):629-633

Yantai Harbour (37°33′15″N , 121°23′42″E) is situated at the north coast of Shandong Peninsula and is a large fishing and commercial port of North China. From June 1983 to May 1984, a yearly panel test on fouling organisms was carried out at the non-fixed jetties in the inner and outer harbour. The test method was according to "the Marine Survey  相似文献   

On the radiation and diffraction of linear water waves by an infinitely long rectangular structure submerged in oblique seas   总被引：1，自引：0，他引：1  

Y.H. Zheng  P.F. Liu  Y.M. Shen  B.J. Wu  S.W. Sheng 《Ocean Engineering》2007,34(3-4):436-450

The radiation and diffraction of linear water waves by an infinitely long rectangular structure submerged in oblique seas of finite depth is investigated. The analytical expressions for the radiated and diffracted potentials are derived as infinite series by use of the method of separation of variables. The unknown coefficients in the series are determined by the eigenfunction expansion matching method. The expressions for wave forces, hydrodynamic coefficients and reflection and transmission coefficients are given and verified by the boundary element method. Using the present analytical solution, the hydrodynamic influences of the angle of incidence, the submergence, the width and the thickness of the structure on the wave forces, hydrodynamic coefficients, and reflection and transmission coefficients are discussed in detail.  相似文献